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Cytochrome P450 monooxygenase specific activity reduction in wheat Triticum aestivum induced by soil roxithromycin stress

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Frontiers of environmental Science & engineering 2016年第2期10卷 270-275页

作者： Kangxin HE Qixing ZHOU MOE Key Laboratory of Pollution Processes and environmental Criteria/tianjin engineering center of environmental diagnosis and contamination remediation College of Environmental Science and Engineering Nankai University Tianjin 300071 China

Roxithromycin, livestock growth promoter, as widely used medicine and arouses concern because its occurrence and persistence in soil environments. However, effects of roxithromycin in higher plants are still vague. Accordingly, we hypothesized that roxithromycin-con- taminated soil may exhibits ecotoxicological effects in wheat （Triticum aestivum）. In this study, effects induced by a gradient concentration of roxithromycin stress （0.01, 0.1, 1, 10, and 100 ***-1） was investigated in a 7-d soil test in T. aestivum. Results indicated that the specific activity of cytochrome P450 （CYP450） monooxygenase was decreased dramatically with the concentration of roxithro- mycin in soil. The IC50 value was ***-1 of roxithromycin. On the contrary, the growth related endpoints （i.e., the germination percentage, the biomass and the height）, the content related endpoints （i.e., soluble protein content and CYP450 content）, and the superoxide dismutase （SOD） activity failed to reveal the roxithromy- cin-induced effects. Further analysis revealed that the CYP450 monooxygenase specific activity reduction was enzymatic mechanism mediated, other than oxidative stress induced. We conclude that the soil roxithromycin declined the CYP450 monooxygenase activity in T. aestivum by the inhibition of the enzymatic mechanism. Further efforts can include, but are not limited to, investigation of joint effects induced by combined exposure of roxithromycin and the pesticides and evalua- tion of the similar effects in other higher plants.

关键词： roxithromycin toxic effect cytochrome P450monooxygenase soil environment Triticum aestivum,biomarker

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Electrochemical filtration for drinking water purification:A review on membrane materials,mechanisms and roles

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Journal of environmental Sciences 2024年第7期141卷 102-128页

作者： Yuying Qi Donghao Li Shixuan Zhang Fengxiang Li Tao Hua College of environmental Science and engineering Nankai UniversityTianjin 300350China Key Laboratory of Pollution Process and environmental Criteria Ministry of EducationTianjin 300350China tianjin engineering center of environmental diagnosis and contamination remediation Tianjin 300350China

Electrochemical filtration can not only enrich low concentrations of pollutants but also produce reactive oxygen species to interact with toxic pollutants with the assistance of a power supply,making it an effective strategy for drinking water *** addition,the application of electrochemical filtration facilitates the reduction of pretreatment procedures and the use of chemicals,which has outstanding potential for maximizing process simplicity and reducing operating costs,enabling the production of safe drinking water in smaller *** recent years,the research on electrochemical filtration has gradually increased,but there has been a lack of attention on its application in the removal of low concentrations of pollutants from low conductivity *** this review,membrane substrates and electrocatalysts used to improve the performance of electrochemical membranes are briefly ***,the application prospects of emerging single-atom catalysts in electrochemical filtration are also ***,several electrochemical advanced oxidation processes coupled with membrane filtration are described,and the related working mechanisms and their advantages and shortcomings used in drinking water purification are ***,the roles of electrochemical filtration in drinking water purification are presented,and the main problems and future perspectives of electrochemical filtration in the removal of low concentration pollutants are discussed.

关键词： Electrochemical filtration Drinking water Conductive membrane Low concentration Electrocatalyst

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Biochar-mediated regulation of greenhouse gas emission and toxicity reduction in bioremediation of organophosphorus pesticide-contaminated soils

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Chinese Journal of Chemical engineering 2018年第12期26卷 2592-2600页

作者： Meinan Zhen Benru Song Xiaomei Liu Radhika Chandankere Jingchun Tang Key Laboratory of Pollution Processes and environmental Criteria (Ministry of Education) Tianjin Engineering Center of Environmental Diagnosis and Contamination Remediation College of Environmental Science and Engineering Nankai University Department of Building Civil and Environmental Engineering Concordia University

Organophosphorus pesticides(OPPs) are a set of toxic persistent organic pollutants(POPs) present in the environment. Recently, biochar-mediated bioremediation has exhibited many advantages over conventional methods for the remediation of pesticide-contaminated soil. In the present study, biochar and nitrogen fertilizer(NH_4NO_3)were employed to remediate OPP-contaminated soil and the greenhouse gas(GHG) emission during 90 days of incubation was investigated. After thermal desorption treatment, the content of organophosphorus pesticides reduced from 175.61 μg·kg^(-1) to 62.68 μg·kg^(-1). The addition of NH_4NO_3 in the following bioremediation led to larger reduction(34.35%) of the pesticide concentration than that of biochar(31.90%) for the contaminated soils with thermal desorption treatment, while the simultaneous addition of biochar and NH_4NO_3 led to the largest reduction of pesticide concentration(11.07%) for the soil without thermal desorption treatment. The addition of biochar and NH_4NO_3 only slightly increased the emission rate of GHGs from the soil without thermal treatment,but remarkably increased the emission rate of GHGs from the soil after thermal treatment. In most cases, the addition of NH_4NO_3 is more effective than biochar to promote the degradation of pesticide, but also exhibited higher GHG emission. The microbial community analysis suggests that the enhanced degradation of pesticide is mainly owing to the increased activity of microorganism.

关键词： Organophosphorus pesticides Biochar NH3NO4 remediation Greenhouse gas Microbial community

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Carbon Fibers for Bioelectrochemical:Precursors,Bioelectrochemical System,and Biosensors

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Advanced Fiber Materials 2023年第3期5卷 699-730页

作者： Donghao Li Yimeng Feng Fengxiang Li Jingchun Tang Tao Hua College of environmental Science and engineering Nankai University38 Tongyan RoadTianjin 300350China Key Laboratory of Pollution Process and environmental Criteria Tianjin Engineering Center of Environmental Diagnosis and Contamination RemediationTianjin 300350China

Carbon fibers(CFs)demonstrate a range of excellent properties including(but not limited to)microscale diameter,high hardness,high strength,light weight,high chemical resistance,and high temperature ***,it is necessary to summarize the application market of *** with good physical and chemical properties stand out among many *** is believed that highly fibrotic CFs will play a crucial *** review first introduces the precursors of CFs,such as polyacrylonitrile,bitumen,and *** this review introduces CFs used in BESs,such as electrode materials and modification strategies of MFC,MEC,MDC,and other cells in a large ***,CFs in biosensors including enzyme sensor,DNA sensor,immune sensor and implantable sensor are ***,we discuss briefly the challenges and research directions of CFs application in BESs,biosensors and more fields.

关键词： Carbon fiber material Bioelectrochemistry Microbial fuel cell Microbial electrolysis cell Modified Biosensors

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Vertical response of microbial community and degrading genes to petroleum hydrocarbon contamination in saline alkaline soil

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Journal of environmental Sciences 2019年第7期31卷 80-92页

作者： Qinglong Liu Jingchun Tang Xiaomei Liu Benru Song Meinan Zhen Nicholas J.Ashbolt College of environmental Science and engineering Nankai University Key Laboratory of Pollution Processes and environmental Criteria (Ministry of Education) tianjin engineering Research center of environmental diagnosis and contamination remediation School of Public Health University of Alberta

A column microcosm was conducted by amending crude oil into Dagang Oilfield soil to simulate the bioremediation process. The dynamic change of microbial communities and metabolic genes in vertical depth soil from 0 to 80 cm were characterized to evaluate the petroleum degradation potential of indigenous microorganism. The influence of environmental variables on the microbial responds to petroleum contamination were analyzed. Degradation extent of 42.45% of n-alkanes(C8–C40) and 34.61% of 16ΣPAH were reached after 22 weeks. Relative abundance of alkB, nah, and phe gene showed about 10-fold increment in different depth of soil layers. Result of HTS profiles demonstrated that Pseudomonas, Marinobacter and Lactococcus were the major petroleum-degrading bacteria in0–30 and 30–60 cm depth of soils. Fusarium and Aspergillus were the dominant oil-degrading fungi in the 0–60 cm depth of soils. In 60–80 cm deep soil, anaerobic bacteria such as Bacteroidetes, Lactococcus, and Alcanivorax played important roles in petroleum *** analysis(RDA) and correlation analysis demonstrated that petroleum hydrocarbons(PHs) as well as soil salinity, clay content, and anaerobic conditions were the dominant effect factors on microbial community compositions in 0–30, 30–60, and 60–80 cm depth of soils, respectively.

关键词： Column microcosm Molecular analyses Petroleum hydrocarbons Microbial community Catabolic genes Saline alkaline soil

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High pyrolysis temperature biochar reduced the transport of petroleum degradation bacteria Corynebacterium variabile HRJ4 in porous media

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Journal of environmental Sciences 2021年第2期33卷 228-239页

作者： Saisai Guo Xiaomei Liu Hang Zhao Lan Wang Jingchun Tang College of environmental Science and engineering Nankai UniversityTianjin 300350China Key Laboratory of Pollution Processes and environmental Criteria(Ministry of Education) Tianijin 300350China tianjin engineering Research center of Enuironmental diagnosis and contamination remediation Tianjin 300350China

Biochar has been widely applied for the remediation of petroleum-contaminated ***,the effect of biochar on the transport of petroleum degradation bacteria has not been studied.A typical Gram-positive petroleum degradation bacteria-Corynebacterium variabile HRJ4 was used to study the effect of different biochars on bacterial transport and *** indicated that the addition of biochar in sand was effective for reducing the transport of bacteria and poplar sawdust biochar(PSBC)had a stronger hinder effect than corn straw biochar(CSBC).The hindrance was more evident with pyrolysis temperature of biochar raised from 300℃ to 600℃,which was attributed to the increase of specific surface area(309 times).The hindrance effect also enhanced with higher application rate of ***,the reduction of HRJ4 transport was more obvious in higher(25 mmol/L)concentration of Na Cl solution owing to electrostatic attraction *** adsorption of biochar to HRJ4 was defined to contribute to the hindrance of HRJ4 transport *** the influence of feedstocks and pyrolysis temperature on HRJ4 transport,it suggested that specific surface area had the greatest effect on HRJ4 transport,and pore-filling,electrostatic force also contributed to HRJ4 retained in quartz sand *** last,phenol transportation experiment indicated that the restriction of biochar on HRJ4 enhanced the phenol removal rate in the *** study provides a theoretical basis for the interaction of biochar and bacteria,which is vital for the remediation of oil-contaminated soil and groundwater in the field.

关键词： Gram-positive bacteria Feedstocks HRJ4 Transport Retention

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Biochar-derived dissolved organic matters influencing bacterium characteristics during biodegradation of sulfamethoxazole and chloramphenicol under alternation of visible and avoiding light

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Biochar 2023年第1期5卷 160-174页

作者： Fang Yang Yanan Xue Yue Gao Qing Zhu Cuiping Wang Hongwen Sun Key Laboratory of Pollution Processes and environmental Criteria Ministry of EducationTianjin Key Laboratory of Environmental Remediation and Pollution ControlCollege of Environmental Science and EngineeringNankai UniversityTianjin 300071People’s Republic of China tianjin engineering center of environmental diagnosis and contamination remediation Tianjin 300071People’s Republic of China

The influence of biochar-released dissolved organic matter(BDOM)on the transcription of gene(DEG)in Pseudomonas stutzeri and Shewanella putrefacien during sulfamethoxazole(SMX)and chloramphenicol(CAP)biodegradation under visible light was investigated in this *** results indicated that BDOM components would be nutrients for bacterial amplification and growth under the culture conditions of xenon lamp irradiation and avoiding light,especially BDOM from low ***,visible light irradiation would improve the saturated fatty acid by stimulating the cell membrane of the microorganism,thus promoting the biodegradation of antibiotics through altering *** and *** reoxidative and catabolism processes and significantly inhabiting the copy number of their ***,the upregulated genes and enzymes related to SMX and CAP-metabolic and catabolic processes were enriched,which were involved in the pathways of biodegradation,further improving biodegradation *** particular,interaction network analysis between the top 100 dominant functional genes from *** and *** and the molecular types of BDOM,e.g.,CHO,CHON,and CHOS(p<0.05),indicated that the genes of molecular function showed a high positive or negative correlation with the CHO type of *** results revealed that the CHO type of BDOM affected the functional genes of molecular function,cellular component,and biological process from *** and ***,influencing the biodegradation of SMX and *** study provided an basis for BDOM playing a role in antibiotic removal from the aqueous solution using biochar combined with photobiodegradation.

关键词： Biochar-derived dissolved organic matters Molecular characteristics Antibiotics degradation Transcriptome Functional genes

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Graphitic carbon nitride/biochar composite synthesized by a facile ball-milling method for the adsorption and photocatalytic degradation of enrofloxacin

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Journal of environmental Sciences 2021年第5期33卷 93-107页

作者： Yao Xiao Honghong Lyu Chengliang Yang Beibei Zhao Lan Wang Jingchun Tang School of Energy and environmental engineering Hebei University of TechnologyTianjin 300401China School of Civil and Transportation engineering Hebei University of TechnologyTianjin 300401China Key Laboratory of Pollution Processes and environmental Criteria(Ministry of Education) Tianjin Engineering Center of Environmental Diagnosis and Contamination RemediationCollege of Environmental Science and EngineeringNankai UniversityTianjin 300350China BCIG environmental remediation Co. LtdTianjin 300042China

In order to enhance the removal performance of graphitic carbon nitride(g-C_(3)N_(4)) on organic pollutant, a simultaneous process of adsorption and photocatalysis was achieved via the compounding of biochar and g-C_(3)N_(4). In this study, g-C_(3)N_(4) was obtained by a condensation reaction of melamine at 550 °C. Then the g-C_(3)N_(4)/biochar composites were synthesized by ball milling biochar and g-C_(3)N_(4) together, which was considered as a simple, economical, and green strategy. The characterization of resulting g-C_(3)N_(4)/biochar suggested that biochar and g-C_(3)N_(4) achieved effective linkage. The adsorption and photocatalytic performance of the composites were evaluated with enrofloxacin(EFA) as a model pollutant. The result showed that all the g-C_(3)N_(4)/biochar composites displayed higher adsorption and photocatalytic performance to EFA than that of pure g-C_(3)N_(4). The 50% g-C_(3)N_(4)/biochar performed best and removed 45.2% and 81.1% of EFA(10 mg/L) under darkness and light with a dosage of 1 mg/mL, while g-C_(3)N_(4) were 19.0% and 27.3%, respectively. Besides, 50% g-C_(3)N_(4)/biochar showed the highest total organic carbon(TOC) removal efficiency(65.9%). Radical trapping experiments suggested that superoxide radical( ·O_(2)^(-)) and hole(h~+) were the main active species in the photocatalytic process. After 4 cycles, the composite still exhibited activity for catalytic removal of EFA.

关键词： Biochar g-C_(3)N_(4) Ball milling Adsorption Photocatalysis

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A novel bioremediation strategy for petroleum hydrocarbon pollutants using salt tolerant Corynebacterium variabile HRJ4 and biochar

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Journal of environmental Sciences 2016年第9期28卷 7-13页

作者： Hairong Zhang Jingchun Tang Lin Wang Juncheng Liu Ranjit Gajanan Gurav Kejing Sun College of environmental Science and engineering Nankai University Tianjin 300071 China tianjin engineering center of environmental diagnosis and contamination remediation Tianjin 300071 China Key Laboratory of Pollution Processes and environmental Criteria (Ministry of Education) Tianjin 300071 China State Key Laboratory of Enuironmental Criteria and Risk Assessment Chinese Research Academy of Environmental SciencesBeijing 100012 China

The present work aimed to develop a novel strategy to bioremediate the petroleum hydrocarbon contaminants in the *** tolerant bacterium was isolated from Dagang oilfield,China and identified as Corynebacterium variabile HRJ4 based on 16 S r RNA gene sequence *** bacterium had a high salt tolerant capability and biochar was developed as carrier for the *** bacteria with biochar were most effective in degradation of n-alkanes（C16,C18,C19,C26,C28） and polycyclic aromatic hydrocarbons（NAP,PYR） *** result demonstrated that immobilization of *** HRJ4 with biochar showed higher degradation of total petroleum hydrocarbons（THPs） up to 78.9%after 7-day of incubation as compared to the free leaving *** approach of this study will be helpful in clean-up of petroleum-contamination in the environments through bioremediation process using eco-friendly and cost effective materials like biochar.

关键词： Bioremediation Biochar Corynebacterium variabile HRJ4 Immobilization Petroleum hydrocarbons

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Assessment of potential soybean cadmium excluder cultivars at different concentrations of Cd in soils

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Journal of environmental Sciences 2015年第9期27卷 108-114页

作者： Yang Zhi Kangxin He Ting Sun Yongqiang Zhu Qixing Zhou College of Sciences Northeastern University School of Medical Devices Shenyang Pharmaceutical University MOE Key Laboratory of Pollution Processes and environmental Criteria Tianjin Engineering Center of Environmental Diagnosis and Contamination Remediation College of Environmental Science and Engineering Nankai University Techcomp Scientific Instrument Company Limited

The selection of cadmium-excluding cultivars has been used to minimize the transfer of cadmium into the human food chain. In this experiment, five Chinese soybean plants were grown in three soils with different concentrations of Cd（0.15, 0.75 and 1.12 mg/kg）.Variations in uptake, enrichment, and translocation of Cd among these soybean cultivars were studied. The results indicated that the concentration of Cd in seeds that grew at1.12 mg/kg Cd in soils exceeded the permitted maximum levels in soybeans. Therefore, our results indicated that even some soybean cultivars grown on soils with permitted levels of Cd might accumulate higher concentrations of Cd in seeds that are hazardous to human health. The seeds of these five cultivars were further assessed for interactions between Cd and other mineral nutrient elements such as Ca, Cu, Fe, Mg, Mn and Zn. High Cd concentration in soil was found to inhibit the uptake of Mn. Furthermore, Fe and Zn accumulations were found to be enhanced in the seeds of all of the five soybean cultivars in response to high Cd concentration. Cultivar Tiefeng 31 was found to fit the criteria for a Cd-excluding cultivar under different concentrations of Cd in soils.

关键词： Glycine max L. Cd-excluding cultivar Food safety Mineral nutrient element

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