检索结果-南通市图书馆

Expression of cyclin-dependent kinase 9 is positively correlated with the autophagy level in colon cancer

World Journal of Gastrointestinal Oncology 2024年第2期16卷 314-330页

作者： Lei Zheng Jia Lu Da-Lu Kong Department of Colorectal Cancer Surgery National Clinical Research Center for CancerKey Laboratory of Cancer Immunology and Biotherapy of TianjinTianjin Key Laboratory of Digestive CancerKey Laboratory of Cancer Prevention and Therapy of TianjinTianjin’s Clinical Research Center for CancerTianjin Medical University Cancer Institute and HospitalTianjin 300060China Department of Infection Management National Clinical Research Center for CancerKey Laboratory of Cancer Immunology and Biotherapy of TianjinTianjin Key Laboratory of Digestive CancerKey Laboratory of Cancer Prevention and Therapy of TianjinTianjin’s Clinical Research Center for CancerTianjin Medical University Cancer Institute and HospitalTianjin 300060China

BACKGROUND Cyclin-dependent kinase 9(CDK9)expression and autophagy in colorectal cancer(CRC)tissues has not been widely ***9,a key regulator of transcription,may influence the occurrence and progression of *** expression of auto-phagy-related genes BECN1 and drug resistance factor ABCG2 may also play a role in *** normal physiological conditions,autophagy can inhibit tumorigenesis,but once a tumor forms,autophagy may promote tumor ***,understanding the relationship between autophagy and cancer,partic-ularly how autophagy promotes tumor growth after its formation,is a key motivation for this *** To investigate the relationship between CDK9 expression and autophagy in CRC,assess differences in autophagy between left and right colon cancer,and analyze the associations of autophagy-related genes with clinical features and *** We collected tumor tissues and paracarcinoma tissues from colon cancer patients with liver metastasis to observe the level of autophagy in tissues with high levels of CDK9 and low levels of *** also collected primary tissue from left and right colon cancer patients with liver metastasis to compare the autophagy levels and the expression of BECN1 and ABCG2 in the tumor and paracarcinoma *** The incidence of autophagy and the expression of BECN1 and ABCG2 were different in left and right colon cancer,and autophagy might be involved in the occurrence of chemotherapy *** analysis of the rela-tionship between the expression of autophagy-related genes CDK9,ABCG2,and BECN1 and the clinical features and prognosis of colorectal cancer showed that the high expression of CDK9 indicated a poor prognosis in colorectal *** This study laid the foundation for further research on the combination of CDK9 inhibitors and autophagy inhibitors in the treatment of patients with CRC.

关键词： Autophagy Tumorigenesis Tumor tissue Paracarcinoma tissue Expression Left/right colon cancer

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Structural and functional insights into the 20-O-methyltransferase of SARS-CoV-2

引用

Virologica Sinica 2024年第4期39卷 619-631页

作者： Jikai Deng Feiyu Gong Yingjian Li Xue Tan Xuemei Liu Shimin Yang Xianying Chen Hongyun Wang Qianyun Liu Chao Shen Li Zhou Yu Chen State Key laboratory of Virology RNA InstituteCollege of Life Sciences and Frontier Science Center for Immunology and MetabolismWuhan UniversityWuhan430072China Animal Bio-Safety level III laboratory/Institute for Vaccine Research Wuhan University School of MedicineWuhan430071China

A unique feature of coronaviruses is their utilization of self-encoded nonstructural protein 16(nsp16),20-Omethyltransferase(20-O-MTase),to cap their RNAs through ribose 20-O-methylation *** process is crucial for maintaining viral genome stability,facilitating efficient translation,and enabling immune *** considerable advances in the ultrastructure of SARS-CoV-2 nsp16/nsp10,insights into its molecular mechanism have so far been *** this study,we systematically characterized the 20-O-MTase activity of nsp16 in SARS-CoV-2,focusing on its dependence on nsp10 *** observed cross-reactivity between nsp16 and nsp10 in various coronaviruses due to a conserved interaction ***,a single residue substitution(K58T)in SARS-CoV-2 nsp10 restricted the functional activation of MERS-CoV ***,the cofactor nsp10 effectively enhanced the binding of nsp16 to the substrate RNA and the methyl donor Sadenosyl-L-methionine(SAM).Mechanistically,His-80,Lys-93,and Gly-94 of nsp10 interacted with Asp-102,Ser-105,and Asp-106 of nsp16,respectively,thereby effectively stabilizing the SAM binding ***-43 of nsp10 interacted with Lys-38 and Gly-39 of nsp16 to dynamically regulate the RNA binding pocket and facilitate precise binding of RNA to the nsp16/nsp10 *** assessing the conformational epitopes of nsp16/nsp10 complex,we further determined the critical residues involved in 20-O-MTase ***,we utilized an in vitro biochemical platform to screen potential inhibitors targeting 20-O-MTase ***,our results significantly enhance the understanding of viral 20-O methylation process and mechanism,providing valuable targets for antiviral drug development.

关键词： SARS-CoV-2 nsp16/nsp10 20-O-Methyltransferase 3-Dimensional structure Molecular mechanism Inhibitors

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

An optimized high-throughput SARS-CoV-2 dual reporter trans-complementation system for antiviral screening in vitro and in vivo

引用

Virologica Sinica 2024年第3期39卷 447-458页

作者： Yingjian Li Xue Tan Jikai Deng Xuemei Liu Qianyun Liu Zhen Zhang Xiaoya Huang Chao Shen Ke Xu Li Zhou Yu Chen State Key laboratory of Virology RNA InstituteCollege of Life Sciences and Frontier Science Center for Immunology and MetabolismWuhan UniversityWuhan430072China Institute for Vaccine Research at Animal Bio-safety level III laboratory Wuhan University School of MedicineWuhan430071China

The severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is still epidemic around the *** manipulation of SARS-CoV-2 is restricted to biosafety level 3 laboratories(BSL-3).In this study,we developed a SARS-CoV-2ΔN-GFP-HiBiT replicon delivery particles(RDPs)encoding a dual reporter gene,GFP-HiBiT,capable of producing both GFP signal and luciferase *** optimal selection of the reporter gene,GFP-HiBiT demonstrated superior stability and convenience for antiviral ***,we established a RDP infection mouse model by delivering the N gene into K18-hACE2 KI mouse through *** mouse model supports RDP replication and can be utilized for in vivo antiviral *** summary,the RDP system serves as a valuable tool for efficient antiviral screening and studying the gene function of ***,this system can be manipulated in BSL-2 laboratories,decreasing the threshold of experimental requirements.

关键词： SARS-CoV-2 RDP Antiviral evaluation Mouse model BSL-2

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Protective Effect of Silibinin on Lipopolysaccharide-Induced Endotoxemia by Inhibiting Caspase-11-Dependent Cell Pyroptosis

引用

Chinese Journal of Integrative Medicine 2024年第10期30卷 917-926页

作者： OU Jin-ying LIU Shan-hong TANG Dong-kai SHI Ling-zhu YAN Li-jun HUANG Jing-yan ZOU Li-fang QUAN Jing-yu YOU Yan-ting CHEN Yu-yao YU Lin-zhong LU Zi-bin Third level Research laboratory of State Administration of Traditional Chinese Medicine School of Traditional Chinese MedicineSouthern Medical UniversityGuangzhou510515China Guangdong Provincial Key laboratory of Chinese Medicine Pharmaceutics Guangzhou510515China School of Traditional Chinese Medicine Southern Medical UniversityGuangzhou510515China

Objective:To explore the protective effect and the underlying mechanism of silibinin(SIB),one of the active compounds from Silybum marianum(L.)Gaertn in ***:Mouse peritoneal macrophage were isolated via intraperitoneally injection of BALB/c mice with thioglycolate *** viability was assessed using the cell counting kit-8,while cytotoxicity was determined through lactate dehydrogenase cytotoxicity *** protein expressions of interleukin(IL)-1α,IL-1β,and IL-18 were determined by enzyme-linked immunosorbent *** lipopolysaccharide(LPS)levels were measured by employing both the limulus amoebocyte lysate assay and flow ***,proximity ligation assay was employed for the LPS and caspase-11 *** were divided into 4 groups:the control,LPS,high-dose-SIB(100 mg/kg),and low-dose-SIB(100 mg/kg)groups(n=8).Zebrafish were divided into 4 groups:the control,LPS,high-dose-SIB(200μmol/L),and low-dose-SIB(100μmol/L)groups(n=30 for survival experiment and n=10 for gene expression analysis).The expression of caspase-11,gasdermin D(GSDMD),and N-GSDMD was determined by Western blot and the expressions of caspy2,gsdmeb,and IL-1βwere detected using quantitative real-time *** observation was performed through hematoxylineosin staining,and protein levels in bronchoalveolar lavage fluid were quantified using the bicinchoninicacid protein ***:SIB noticeably decreased caspase-11 and GSDMD-mediated pyroptosis and suppressed the secretion of IL-1α,IL-1β,and IL-18 induced by LPS(P<0.05).Moreover,SIB inhibited the translocation of LPS into the cytoplasm and the binding of caspase-11 and intracellular LPS(P<0.05).SIB also attenuated the expression of caspase-11 and N-terminal fragments of GSDMD,inhibited the relative cytokines,prolonged the survival time,and up-regulated the survival rate in the endotoxemia models(P<0.05).Conclusions:SIB can inhibit pyroptosis in the LPS-mediated endotoxemia model,at l

关键词： silibinin caspase-11 endotoxemia pyroptosis inflammation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Hepatic arterial infusion chemotherapy with anti-angiogenesis agents and immune checkpoint inhibitors for unresectable hepatocellular carcinoma and meta-analysis

引用

World Journal of Gastroenterology 2024年第4期30卷 318-331页

作者： Yu-Zhe Cao Guang-Lei Zheng Tian-Qi Zhang Hong-Yan Shao Jia-Yu Pan Zi-Lin Huang Meng-Xuan Zuo Department of Minimally Invasive Interventional therapy Sun Yat-sen University Cancer CenterGuangzhou 510060Guangdong ProvinceChina Department of Minimally Invasive Interventional therapy State Key Laboratory of Oncology in South ChinaGuangzhou 510060Guangdong ProvinceChina Department of Minimally Invasive Interventional therapy Guangdong Provincial Clinical Research Center for CancerGuangzhou 510060Guangdong ProvinceChina

BACKGROUND Hepatic arterial infusion chemotherapy(HAIC)has been proven to be an ideal choice for treating unresectable hepatocellular carcinoma(uHCC).HAIC-based treatment showed great potential for treating ***,large-scale studies on HAIC-based treatments and meta-analyses of first-line treatments for uHCC are *** To investigate better first-line treatment options for uHCC and to assess the safety and efficacy of HAIC combined with angiogenesis inhibitors,programmed cell death of protein 1(PD-1)and its ligand(PD-L1)blockers(triple therapy)under real-world *** Several electronic databases were searched to identify eligible randomized controlled trials for this ***-level pooled analyses of hazard ratios(HRs)and odds ratios(ORs)were *** was a retrospective single-center study involving 442 patients with uHCC who received triple therapy or angiogenesis inhibitors plus PD-1/PD-L1 blockades(AIPB)at Sun Yat-sen University Cancer Center from January 2018 to April *** score matching(PSM)was performed to balance the bias between the *** Kaplan-Meier method and cox regression were used to analyse the survival data,and the log-rank test was used to compare the suvival time between the *** A total of 13 randomized controlled trials were *** alone and in combination with sorafenib were found to be effective treatments(P values for ORs:HAIC,0.95;for HRs:HAIC+sorafenib,0.04).After PSM,176 HCC patients were included in the *** triple therapy group(n=88)had a longer median overall survival than the AIPB group(n=88)(31.6 months vs 14.6 months,Ptherapy is more effective for uHCC patients than AIPB.

关键词： Unresectable hepatocellular carcinoma Hepatic arterial infusion chemotherapy Angiogenesis inhibitors Programmed cell death protein 1 Programmed death ligand 1

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Highly efficient and stable 2D/3D perovskite solar cells based on surface reconstruction and energy level alignment

引用

Science China Chemistry 2024年第9期67卷 3083-3090页

作者： Qiaohui Li Hang Liu Tong Zhou Key laboratory of Rubber-Plastics Ministry of Education/Shandong Provincial Key Laboratory of Rubber-PlasticsQingdao University of Science and TechnologyQingdao 266042China The Centre of Nanoscale Science and Technology and Key laboratory of Functional Polymer Materials Institute of Polymer ChemistryCollege of ChemistryNankai UniversityTianjin 300071China

Realizing simultaneous adjustment of energy levels and work functions in two-dimensional/three-dimensional(2D/3D)perovskite solar cells(PSCs)is a ***,a pseudohalide 3,5-bis(trifluoromethyl)benzylammonium tetrafluoroborate(TFPMABF_(4))was used to react with unreacted Pb I2on the surface of 3D bulky perovskite to form a mixed halide of 2D perovskite denoted(TF-PMA)_(2)FA_(2)Pb_(3)I_(8)(BF_(4))_(2).This novel 2D/3D perovskite enables the simultaneous adjustment of energy levels and work functions on the surface of active *** to the significantly enhanced quality of 2D/3D perovskite film,decreased surface defects and increased charge carrier lifetime,the 2D/3D PSCs exhibit an outstanding power conversion efficiency(PCE)of 25.15%and a high V_(OC)of 1.194 ***,2D/3D PSCs exhibit remarkable enhancements in environmental stability,unencapsulated devices retaining more than 90%of their initial PCE at 50%humidity for 2,280 h.

关键词： 2D/3D perovskite perovskite solar cells organic spacers energy levels alignment

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The risk factors and threshold level of subchronic inhalation exposure of reclaimed water

引用

Journal of Environmental Sciences 2024年第3期137卷 639-650页

作者： Jiayang Kong Yun Lu Yunru Ren Menghao Chen Gang Liu Liangliang Shi State Key Joint laboratory of Environment Simulation and Pollution Control State Environment Protection Key Laboratory of Microorganism Application and Risk ControlSchool of EnvironmentTsinghua UniversityBeijing 100084China

Inhalation of reclaimed water is known to cause lung infammation,and free endotoxins have been shown to be a major risk factor for acute *** exposure has also been shown to induce infammatory responses with visible tissue ***,subchronic risk factors have yet to be identified,and a threshold for the protection of occupational populations during urban reuse is *** this study,potential risk factors in reclaimed water were examined by subchronic exposure with fractionated reclaimed water,and the health risk threshold was tested with a series of diluted reclaimed ***,following a 12-week exposure,macromolecules and microorganisms were found to be two major risk factors in reclaimed water that could cause pulmonary infammation,including increased proportion of polymorphonuclear leukocytes in bronchoalveolar fuid,formation of inducible bronchus-associated lymphoid tissue,and elevation of Immunoglobulin A ***,infammation persisted after a 4-week recovery *** calculated threshold of reclaimed water exposure for mice was 31.8 Endotoxin Unit(EU)/(kg·day)under when exposed to 50%additional relative humidity from reclaimed water at 25℃ for 2 hr/***,the subchronic threshold estimate for humans under the same exposure conditions was found to be 12.2 EU/(kg·day),corresponding to endotoxin levels of 61.7 EU/mL in reclaimed *** threshold level of endotoxin was lower than that in most non-potable reclaimed *** findings of this study suggest that occupational exposure of reclaimed water can serve as a potential risk to workers.

关键词： Reclaimed water Inhalation exposure Risk factor Threshold Infammation Endotoxin

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Natural evidence of coronaviral 2′-O-methyltransferase activity affecting viral pathogenesis via improved substrate RNA binding

引用

Signal Transduction and Targeted therapy 2024年第6期9卷 2819-2834页

作者： Jikai Deng Shimin Yang Yingjian Li Xue Tan Jiejie Liu Yanying Yu Qiang Ding Chengpeng Fan Hongyun Wang Xianyin Chen Qianyun Liu Xiao Guo Feiyu Gong Li Zhou Yu Chen State Key laboratory of Virology RNA InstituteCollege of Life Sciences and Frontier Science Center for Immunology and MetabolismWuhan UniversityWuhanChina School of Medicine Tsinghua UniversityBeijingChina School of Basic Medical Sciences Wuhan UniversityWuhanChina Animal Bio-Safety level Ⅲ laboratory/Institute for Vaccine Research Wuhan University School of MedicineWuhanChina

Previous studies through targeted mutagenesis of K-D-K-E motif have demonstrated that 2′-O-MTase activity is essential for efficient viral replication and immune ***,the K-D-K-E catalytic motif of 2′-O-MTase is highly conserved across numerous viruses,including flaviviruses,vaccinia viruses,coronaviruses,and extends even to ***,we observed a stronger 2′-O-MTase activity in SARS-CoV-2 compared to SARS-CoV,despite the presence of a consistently active catalytic *** further identified critical residues(Leu-36,Asn-138 and Ile-153)which served as determinants of discrepancy in 2′-O-MTase activity between SARS-CoV-2 and *** residues significantly enhanced the RNA binding affinity of 2′-O-MTase and boosted its versatility toward RNA *** interest,a triple substitution(Leu^(36)→Ile^(36),Asn^(138)→His^(138),Ile^(153)→Leu^(153),from SARS-CoV-2 to SARS-CoV)within nsp16 resulted in a proportional reduction in viral 2′-O-methylation and impaired viral ***,it led to a significant upregulation of type I interferon(IFN-I)and proinflammatory cytokines both in vitro and vivo,relying on the cooperative sensing of melanoma differentiation-associated protein 5(MDA5)and laboratory of genetics and physiology 2(LGP2).In conclusion,our findings demonstrated that alterations in residues other than K-D-K-E of 2′-O-MTase may affect viral replication and subsequently influence *** changes in nsp16 residues is crucial as it may aid in identifying and assessing future alteration in viral pathogenicity resulting from natural mutations occurring in nsp16.

关键词： pathogenesis impaired alterations

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Reassessing the contributions of terrestrial waters to sea level variations in the South China Sea and its response to alternating ENSO events

引用

Science China Earth Sciences 2024年第7期67卷 2253-2267页

作者： Pengfei YANG Hok Sum FOK Zhongtian MA MOE Key laboratory of Geospace Environment and Geodesy&MNR Key laboratory of Geophysical Geodesy School of Geodesy and GeomaticsWuhan UniversityWuhan 430079China Hubei Luojia laboratory Wuhan 730079China Department of Land Surveying and Geo-Informatics The Hong Kong Polytechnic UniversityHong Kong 999077China

Regional sea level variability is linked to regional terrestrial water and the El Ni?o-Southern Oscillation(ENSO).This study assessed the relationships between the sea level variations in the South China Sea(SCS)and ENSO,the impact of terrestrial water storage(TWS)on non-steric sea level(NSSL),and the contributions of steric sea level(SSL)and NSSL to sea level anomaly(SLA),*** 2003 to 2015,the SLAs exhibited a long-term trend of 6.65±0.78 mm/yr,which was primarily attributed to the ***,during 2003-2015,ENSO events alternating with varying intensities might also be responsible for the unusually high SLA *** to the SSLs,the NSSLs contributed the seasonal signals to the SLAs,while the NSSLs changes were largely explained by the TWS in the Mekong River Basin at the seasonal scale and in the Pearl River Basin and Red River Basin at other time *** contrast to the TWS,the contributions of precipitation and evapotranspiration were relatively minor.A negative correlation between the sea level variations and ENSO was also found,with cross-correlation coefficients between the oceanic Ni?o index and SLAs/SSLs/NSSLs of -0.36/-0.37/-0.62 with lags of 2/3/2 months,*** findings systematically reassessed the contributions of different components to the sea level *** study provided a benchmark for in-depth analysis of the impacts of terrestrial water and other potential causes on sea level rise in the SCS.

关键词： South China Sea Sea level variations Terrestrial water storage ENSO

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Creep constitutive model considering nonlinear creep degradation of fractured rock

引用

International Journal of Mining Science and Technology 2024年第1期34卷 105-116页

作者： Wang Chunping Liu Jianfeng Chen Liang Liu Jian Wang Lu Liao Yilin Beijing Research Institute of Uranium Geology Beijing 100029China CAEA Innovation Center for Geological Disposal of High-level Radioactive Waste Beijing 100029China State Key laboratory of Hydraulics and Mountain River Engineering Sichuan UniversityChengdu 610065China School of Architecture and Civil Engineering Xihua UniversityChengdu 610039China

Stability analysis of underground constructions requires a model study of rock masses’ long-term performance. Creep tests under different stress conditions was conducted on intact granite and granite samples fractured at 30° and 45° angles. The experimental results indicate that the steady creep strain rates of intact and fractured rock present an exponential increase trend with the increase of stress level. A nonlinear creep model is developed based on the experimental results, in which the initial damage caused by fracture together with the damage caused by constant load have been taken into consideration. The fitting analysis results indicated that the model proposed is more accurate at identifying the full creep regions in fractured granite, especially the accelerated stage of creep deformation. The least-square fit error of the proposed creep model is significantly lower than that of Nishihara model by almost an order of magnitude. An analysis of the effects of elastic modulus, viscosity coefficient, and damage factors on fractured rock strain rate and creep strain is conducted. If no consideration is given to the effects of the damage, the proposed nonlinear creep model can degenerate into to the classical Nishihara model.

关键词： Fractured rock Damage Creep Beishan granite Geological disposal

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：