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SIRT1 inhibits apoptosis of human lens epithelial cells through suppressing endoplasmic reticulum stress in vitro and in vivo

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International Journal of Ophthalmology(English edition) 2024年第7期17卷 1205-1216页

作者： Hui Cui Di Sun Sheng Meng Tian-Ju Ma Zi Ye Zhao-Hui Li Medical school of Chinese PLA Beijing 100089China senior Department of Ophthalmology the Third Medical Center of PLA General HospitalBeijing 100853China

AIM:To explore the effect of silent information regulator factor 2-related enzyme 1(SIRT1)on modulating apoptosis of human lens epithelial cells(HLECs)and alleviating lens opacification of rats through suppressing endoplasmic reticulum(ER)***:HLECs(SRA01/04)were treated with varying concentrations of tunicamycin(TM)for 24h,and the expression of SIRT1 and C/EBP homologous protein(CHOP)was assessed using real-time quantitative polymerase chain reaction(RT-PCR),Western blotting,and *** morphology and proliferation was evaluated using an inverted microscope and cell counting kit-8(CCK-8)assay,*** the SRA01/04 cell apoptosis model,which underwent siRNA transfection for SIRT1 knockdown and SRT1720 treatment for its activation,the expression levels of SIRT1,CHOP,glucose regulated protein 78(GRP78),and activating transcription factor 4(ATF4)were *** potential reversal of SIRT1 knockdown effects by 4-phenyl butyric acid(4-PBA;an ER stress inhibitor)was *** vivo,age-related cataract(ARC)rat models were induced by sodium selenite injection,and the protective role of SIRT1,activated by SRT1720 intraperitoneal injections,was evaluated through morphology observation,hematoxylin and eosin(H&E)staining,Western blotting,and ***:SIRT1 expression was downregulated in TMinduced SRA01/04 ***,in SRA01/04 cells,both cell apoptosis and CHOP expression increased with the rising doses of *** stress was stimulated by TM,as evidenced by the increased GRP78 and ATF4 in the SRA01/04 cell apoptosis *** of SIRT1 by siRNA knockdown increased ER stress activation,whereas SRT1720 treatment had opposite results.4-PBA partly reverse the adverse effect of SIRT1 knockdown on *** vivo,SRT1720 attenuated the lens opacification and weakened the ER stress activation in ARC rat ***:SIRT1 plays a protective role against TM-induced apoptosis in HLECs and slows the progression of cataract in

关键词： silent information regulator factor 2-related enzyme 1 endoplasmic reticulum stress apoptosis human lens epithelial cells cataract

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Unconditional and Optimal Pointwise Error Estimates of Finite Difference Methods for the Two-Dimensional Complex Ginzburg-Landau Equation

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Journal of Mathematical Research with Applications 2024年第2期44卷 248-268页

作者： Yue CHENG Dongsheng TANG school of Mathematics and Statistics Nantong University Jiangsu Xinhai senior High school

In this paper, we give improved error estimates for linearized and nonlinear CrankNicolson type finite difference schemes of Ginzburg-Landau equation in two dimensions. For linearized Crank-Nicolson scheme, we use mathematical induction to get unconditional error estimates in discrete L2and H1norm. However, it is not applicable for the nonlinear ***, based on a ‘cut-off ’ function and energy analysis method, we get unconditional L2and H1error estimates for the nonlinear scheme, as well as boundedness of numerical solutions. In addition, if the assumption for exact solutions is improved compared to before, unconditional and optimal pointwise error estimates can be obtained by energy analysis method and several Sobolev inequalities. Finally, some numerical examples are given to verify our theoretical analysis.

关键词： complex Ginzburg-Landau equation finite difference method unconditional convergence optimal estimates pointwise error estimates

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Minimalistic approach to left atrial appendage occlusion guided by cardiac computed tomography angiography

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Journal of Geriatric Cardiology 2024年第4期21卷 431-442页

作者： Xian-Sai MENG Qing-Song WANG Xin-Yan WANG Xu LU Yang MU Jing WANG Ting-Ting SONG Yun-Dai CHEN Tao CHEN Jun GUO Graduate school of Chinese PLA Medical College BeijingChina senior Department of Cardiology the Sixth Med-ical CenterChinese PLA General HospitalBeijingChina

OBJECTIVE To assess the feasibility and safety of the minimalistic approach to left atrial appendage occlusion(LAAO) guided by cardiac computed tomography angiography(CCTA).METHODS Ninety consecutive patients who underwent LAAO, with or without CCTA-guided, were matched(1:2). Each step of the LAAO procedure in the computed tomography(CT) guidance group(CT group) was directed by preprocedural CT planning. In the control group, LAAO was performed using the standard method. All patients were followed up for 12 months, and device surveillance was conducted using *** A total of 90 patients were included in the analysis, with 30 patients in the CT group and 60 matched patients in the control group. All patients were successfully implanted with Watchman devices. The mean ages for the CT group and the control group were 70.0 ± 9.4 years and 68.4 ± 11.9 years(P = 0.52), respectively. The procedure duration(45.6 ± 10.7 min vs. 58.8 ± 13.0 min,P < 0.001) and hospital stay(7.5 ± 2.4 day vs. 9.6 ± 2.8 day, P = 0.001) in the CT group was significantly shorter compared to the control group. However, the total radiation dose was higher in the CT group compared to the control group(904.9 ± 348.0 m Gy vs.711.9 ± 211.2 m Gy, P = 0.002). There were no significant differences in periprocedural pericardial effusion(3.3% vs. 6.3%, P = 0.8) between the two groups. The rate of postprocedural adverse events(13.3% vs. 18.3%, P = 0.55) were comparable between both groups at 12 months *** CCTA is capable of detailed LAAO procedure planning. Minimalistic LAAO with preprocedural CCTA planning was feasible and safe, with shortened procedure time and acceptable increased radiation and contras consumption. For patients with contraindications to general anesthesia and/or transesophageal echocardiography, this promising method may be an alternative to conventional LAAO.

关键词： anesthesia matched approach

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Clinical significance,molecular characterization,and immune microenvironment analysis of coagulation-related genes in clear cell renal cell carcinoma

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Cancer Innovation 2024年第1期3卷 29-49页

作者： Weihao Chen Xupeng Zhao Yongliang Lu Hanfeng Wang Xiyou Wang Yi Wang Chen Liang Zhuomin Jia Wei Ma Department of Urology The Third Medical Center of PLA General HospitalBeijingChina school of Medicine Nankai UniversityTianjinChina Medical Service Department The PLA General HospitalBeijingChina senior Department of Otolaryngology-Head&Neck Surgery The Sixth Medical Center of PLA General HospitalBeijingChina

Background:Numerous studies have revealed a tight connection between tumor development and the coagulation ***,the effects of coagulation on the prognosis and tumor microenvironment(TME)of clear cell renal cell carcinoma(ccRCC)remain poorly ***:We employed the consensus clustering method to characterize distinct molecular subtypes associated with coagulation ***quently,we examined variations in the overall survival(OS),genomic profiles,and TME characteristics between these *** develop a prognostic coagulation-related risk score(CRRS)model,we utilized the least absolute shrinkage and selection operator Cox regression and stepwise multivariate Cox regression *** also created a nomogram to aid in the clinical application of the risk score,evaluating the relationships between the CRRS and the immune microenvironment,responsiveness to immunotherapy,and targeted *** clinical significance of PLAUR and its biological function in ccRCC were also further ***:There were significant differences in clinical features,prognostic stratification,genomic variation,and TME characteristics between the two coagulation-related *** established and validated a CRRS using six coagulation-related genes that can be employed as an effective indicator of risk stratification and prognosis estimation for ccRCC *** variations in survival outcomes were observed between the high-and low-risk *** nomogram was proficient in predicting the 1-,3-,and 5-year ***,the CRRS emerged as a novel tool for evaluating the clinical effectiveness of immunotherapy and targeted treatments in ***,we confirmed upregulated PLAUR expression in ccRCC samples that was significantly correlated with poor patient *** knockdown notably inhibited ccRCC cell proliferation and ***:Our data suggested that CRRS may be employed as a reliable predictive biomarker that can pro

关键词： clear cell renal cell carcinoma coagulation immunotherapy risk score tumor immune microenvironment

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Material-SAM:Adapting SAM for Material XCT

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Computers, Materials & Continua 2024年第3期78卷 3703-3720页

作者： Xuelong Wu Junsheng Wang Zhongyao Li Yisheng Miao Chengpeng Xue Yuling Lang Decai Kong Xiaoying Ma Haibao Qiao school of Materials Science and Engineering Beijing Institute of TechnologyBeijing100081China senior Engineer at the Simulation Center Citic Dicastal Co.Ltd.Qinhuangdao066010China

X-ray Computed Tomography(XCT)enables non-destructive acquisition of the internal structure of materials,and image segmentation plays a crucial role in analyzing material XCT *** paper proposes an image segmentation method based on the Segment Anything model(SAM).We constructed a dataset of carbide in nickel-based single crystal superalloys XCT images and preprocessed the images using median filtering,histogram equalization,and gamma ***,SAM was fine-tuned to adapt to the task of material XCT image segmentation,resulting in *** compared the performance of threshold segmentation,SAM,U-Net model,and *** method achieved 88.45%Class Pixel Accuracy(CPA)and 88.77%Dice Similarity Coefficient(DSC)on the test set,outperforming SAM by 5.25%and 8.81%,respectively,and achieving the highest ***-SAM demonstrated lower input requirements compared to SAM,as it only required three reference points for completing the segmentation task,which is one-fifth of the requirement of ***-SAM exhibited promising results,highlighting its potential as a novel method for material XCT image segmentation.

关键词： Segment Anything model X-ray computed tomography U-Net Ni-based superalloys foundation models

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Flexible assistance strategy of lower limb rehabilitation exoskeleton based on admittance model

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Science China(Technological Sciences) 2024年第3期67卷 823-834页

作者： KOU JianGe WANG YiXuan CHEN ZhenLei SHI Yan GUO Qing XU Meng school of Automation Science and Electrical Engineering Beihang UniversityBeijing 100191China school of Aeronautics and Astronautics University of Electronic Science and Technology of ChinaChengdu 611731China Department of Musculoskeletal Tumor Senior Department of Orthopedicsthe Fourth Medical Centerof PLA GeneralHospitalBeijing 100853China

Exercise training based on exoskeleton is an effective rehabilitation method for stroke patients. However, some rehabilitation exoskeletons still have poor wearable performance and obvious human-robot impedance during training, which easily cause secondary injuries to the patients. In this study, a variable admittance control strategy is proposed to improve the operator's wearable comfort, which can adapt to different operators by regulating admittance parameters. The admittance controller has two feedback loops: the position inner-loop and the admittance outer-loop. Meanwhile, the exoskeleton Lagrange model is constructed with unknown friction disturbance. By using Lyapunov technique and backstepping with state observer, the joint position error of exoskeleton is convergence into a zero neighborhood. The effectiveness of the proposed admittance controller is verified by both simulation and experiment. The tracking error of hip and knee joint is less than 4 degrees while the human-robot interaction torque is constrained in a tolerable range of the operator.

关键词： human-robot interaction exoskeleton backstepping admittance control

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Solid-state atomic hydrogen as a broad-spectrum RONS scavenger for accelerated diabetic wound healing

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National Science Review 2024年第2期11卷 196-206页

作者： Man Luo Qin Wang Gang Zhao Wei Jiang Cici Zeng Qingao Zhang Ruyu Yang Wang Dong Yunxi Zhao Guozhen Zhang Jun Jiang Yucai Wang Qing Zhu Key Laboratory of Precision and Intelligent Chemistry School of Chemistry and Materials Science University of Science and Technology of China Department of Radiology The First Affiliated Hospital of University of Science and Technology of China Division of Life Sciences and Medicine University of Science and Technology of China Shenzhen senior High school Institute of Intelligent Innovation Henan Academy of Sciences

Hydrogen therapy shows great promise as a versatile treatment method for diseases associated with the overexpression of reactive oxygen and nitrogen species（RONS）. However, developing an advanced hydrogen therapy platform that integrates controllable hydrogen release, efficient RONS elimination, and biodegradability remains a giant technical challenge. In this study, we demonstrate for the first time that the tungsten bronze phase H0.53WO3（HWO） is an exceptionally ideal hydrogen carrier, with salient features including temperature-dependent highly-reductive atomic hydrogen release and broad-spectrum RONS scavenging capability distinct from that of molecular hydrogen. Moreover, its unique p H-responsive biodegradability ensures post-therapeutic clearance at pathological sites. Treatment with HWO of diabetic wounds in an animal model indicates that the solid-state atomic H promotes vascular formation by activating M2-type macrophage polarization and anti-inflammatory cytokine production, resulting in acceleration of chronic wound healing. Our findings significantly expand the basic categories of hydrogen therapeutic materials and pave the way for investigating more physical forms of hydrogen species as efficient RONS scavengers for clinical disease treatment.

关键词： atomic hydrogen RONS scavenger inflammatory regulation diabetic wound healing hydrogen therapy

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Cost-Effective Method of Gene Synthesis by Sequencing from Microchip-Derived Oligos for Droplet Cloning

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Advances in Bioscience and Biotechnology 2024年第8期15卷 474-485页

作者： Kimberly Wang Jericho senior High school Jericho NY USA

Gene synthesis has provided important contributions in various fields including genomics and medicine. Current genes are 7 - 30 cents depending on the assembly and sequencing methods performed. Demand for gene synthesis has been increasing for the past few decades, yet available methods remain expensive. A solution to this problem involves microchip-derived oligonucleotides (oligos), an oligo pool with a substantial number of oligo fragments. Microchips have been proposed as a tool for gene synthesis, but this approach has been criticized for its high error rate during sequencing. This study tests a possible cost-effective method for gene synthesis utilizing fragment assembly and golden gate assembly, which can be employed for quicker manufacturing and efficient execution of genes in the near future. The droplet method was tested in two trials to determine the viability of the method through the accuracy of the oligos sequenced. A preliminary research experiment was performed to determine the efficacy of oligo lengths ranging from two to four overlapping oligos through Gibson assembly. Of the three oligo lengths tested, only two fragment oligos were correctly sequenced. Two fragment oligos were used for the second experiment, which determined the efficacy of the droplet method in reducing gene synthesis cost and speed. The first trial utilized a high-fidelity polymerase and resulted in 3% correctly sequenced oligos, so the second trial utilized a non-high-fidelity polymerase, resulting in 8% correctly sequenced oligos. After calculating, the cost of gene synthesis lowers down to 0.8 cents/base. The final calculated cost of 0.8 cents/base is significantly cheaper than other manufacturing costs of 7 - 30 cents/base. Reducing the cost of gene synthesis provides new insight into the cost-effectiveness of present technologies and protocols and has the potential to benefit the fields of bioengineering and gene therapy.

关键词： Cost-Effective Gene Synthesis Microchip Oligo Droplet Cloning

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The metabolic profiling of Chinese yam fermented by Saccharomyces boulardii and the biological activities of its ethanol extract in vitro

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Food Science and Human Wellness 2024年第5期13卷 2718-2726页

作者： Xue Li Yiwen Shao Limin Hao Qiaozhen Kang Xiaolong Wang Jiaqing Zhu Changcheng Zhao Yanling Shi Jike Lu Juanjuan Yi school of Life Sciences Zhengzhou UniversityZhengzhou 450001China Food Laboratory of Zhongyuan Zhengzhou UniversityLuohe 462300China Systems Engineering Institute Academy of Military Sciences(AMS)Beijing 100010China Henan Yinfeng Biological Engineering Technology Co. Ltd.Zhengzhou 450000China

Chinese yam(Dioscorea opposita Thunb.),as one of the medicinal and edible homologous plants,is rich in various nutrients and functional *** this study,Chinese yam fermented by Saccharomyces boulardii was performed to investigate its bioactive components and metabolic *** then,the main bioactive components and biological activities of fermented Chinese yam ethanol extract(FCYE)were *** showed that there were 49 up-regulated metabolites and 52 down-regulated metabolites in fermented Chinese yam compared to unfermented Chinese ***,corresponding metabolic pathways analysis initially revealed that the distribution of bioactive substances was concentrated on alcoholsoluble small molecular ***,the total polyphenol content and the total flavonoid content in FCYE were significantly increased,and the corresponding antioxidant and immunomodulatory activities in vitro were also significantly *** study provided a new reference for the comprehensive utilization of Chinese yam and laid a theoretical foundation for the development and application of natural probiotic-fermented products.

关键词： Chinese yam Saccharomyces boulardii Metabolites Antioxidant Immunomodulation

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The Limbal Niche and Its Role in Maintaining Corneal Regeneration

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Open Journal of Ophthalmology 2024年第1期14卷 76-91页

作者： Jaysukh P. Singh Syosset senior High school Syosset New York USA

In recent years, stem cells have been a focal point in research designed to evaluate the efficacy of ophthalmologic therapies, specifically those for corneal conditions. The corneal epithelium is one of the few regions of the body that maintains itself using a residual stem cell population within the adjacent limbus. Stem cell movement has additionally captivated the minds of researchers due to its potential application in different body regions. The cornea is a viable model for varying methods to track stem cell migratory patterns, such as lineage tracing and live imaging from the limbus. These developments have the potential to pave the way for future therapies designed to ensure the continuous regeneration of the corneal epithelium following injury via the limbal stem cell niche. This literature review aims to analyze the various methods of imaging used to understand the limbal stem cell niche and possible future directions that might be useful to consider for the better treatment and prevention of disorders of the cornea and corneal epithelium. .

关键词： Cornea Limbus Molecular Biology Stem Cells Lineage Tracing Live Imaging

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