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Expression and significance of pigment epithelium-derived factor and vascular endothelial growth factor in colorectal adenoma and cancer

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World Journal of Gastrointestinal Oncology 2024年第3期16卷 670-686页

作者： Ye Yang Wu Wen Feng-Lin Chen Ying-Jie Zhang Xiao-Cong Liu Xiao-Yan Yang shan-shan Hu Ye Jiang Jing Yuan Digestive Diseases Chengdu Qingbaijiang District People's HospitalChengdu 610300Sichuan ProvinceChina Digestive Diseases Chengdu Second People’s HospitalChengdu 610000Sichuan ProvinceChina Graduate school Chengdu Medical CollegeChengdu 610000Sichuan ProvinceChina

BACKGROUND The incidence and mortality of colorectal cancer(CRC)are among the highest in the world,and its occurrence and development are closely related to tumor *** the balance between pigment epithelium-derived factors(PEDF)that inhibit angiogenesis and vascular endothelial growth factors(VEGF)that stimulate angiogenesis is broken,angiogenesis is out of control,resulting in tumor ***,it is very necessary to find more therapeutic targets for CRC for early intervention and later *** To investigate the expression and significance of PEDF,VEGF,and CD31-stained microvessel density values(CD31-MVD)in normal colorectal mucosa,adenoma,and *** In this case-control study,we collected archived wax blocks of specimens from the Digestive Endoscopy Center and the General surgery Department of Chengdu second people's hospital from April 2022 to October *** cases of specimen wax blocks were selected as normal intestinal mucosa confirmed by electronic colonoscopy and concurrent biopsy(normal control group),50 cases of specimen wax blocks were selected as colorectal adenoma confirmed by electronic colonoscopy and pathological biopsy(adenoma group),and 50 cases of specimen wax blocks were selected as CRC confirmed by postoperative pathological biopsy after inpatient operation of general surgery(CRC group).An immunohistochemical staining experiment was carried out to detect PEDF and VEGF expression in three groups of specimens,analyze their differences,study the relationship between the two and clinicopathological factors in CRC group,record CD31-MVD in the three groups,and analyze the correlation of PEDF,VEGF,and CD31-MVD in the colorectal adenoma group and the CRC *** F test or adjusted F test is used to analyze measurement data ***-Wallis rank sum test was used between groups for ranked *** chi-square test,adjusted chi-square test,or Fisher's exact test were used to compare the rates betwe

关键词： Pigment epithelium-derived factors Vascular endothelial growth factor Microvessel density Colorectal adenoma Colorectal cancer Targeted therapy

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Effective Nucleic Acid Contamination Disinfection in Laboratory settings using Ozone Gas

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Biomedical and Environmental sciences 2024年第9期37卷 1095-1100页

作者： Yingyi Long Linlin Liang Xingyan Zhou Fei Ren Lu Wang Peng Zhang Jing Wang Department of Laboratory The Second People’s Hospital of Jiulongpo DistrictChongqing 400030China Jiulongpo district Center for Disease Control and Prevention Chongqing 400030China

The Nucleic Acid Laboratory,a division of the Clinical Laboratory Department,is responsible for performing real-time PCR(qPCR)assays to rapidly detect infectious diseases,genetic disorders,and more[1].As the gold standard,qPCR has played an indispensable role in diagnosing pandemic infections,such as the coronavirus disease 2019(COVID-19)[2].

关键词： qPCR indispensable performing

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Clinical risk factors for preterm birth and evaluating maternal psychology in the postpartum period

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World Journal of Psychiatry 2024年第5期14卷 661-669页

作者： Jia-Jun Chen Xue-Jin Chen Qiu-Min she Jie-Xi Li Qiu-Hong Luo Department of Clinical Laboratory Shenzhen Bao’an District Songgang People’s HospitalShenzhen 518000Guangdong ProvinceChina Department of Otolaryngology Head and Neck surgery Outpatient Shenzhen Children’s HospitalShenzhen 518000Guangdong ProvinceChina Department of Prevention and Health Care Shenzhen Bao’an District Songgang People’s HospitalShenzhen 518000Guangdong ProvinceChina Department of Obstetrics Shenzhen Bao’an District Songgang People’s HospitalShenzhen 518000Guangdong ProvinceChina

BACKGROUND Although the specific pathogenesis of preterm birth(PTB)has not been thoroughly clarified,it is known to be related to various factors,such as pregnancy complications,maternal socioeconomic factors,lifestyle habits,reproductive history,environmental and psychological factors,prenatal care,and nutritional *** has serious implications for newborns and families and is associated with high mortality and ***,the prediction of PTB risk can facilitate early intervention and reduce its resultant adverse *** To analyze the risk factors for PTB to establish a PTB risk prediction model and to assess postpartum anxiety and depression in *** A retrospective analysis of 648 consecutive parturients who delivered at shenzhen Bao’an district songgang people’s hospital between January 2019 and January 2022 was *** to the diagnostic criteria for premature infants,the parturients were divided into a PTB group(n=60)and a full-term(FT)group(n=588).Puerperae were assessed by the self-rating Anxiety scale(sAs)and self rating Depression scale(sDs),based on which the mothers with anxiety and depression symptoms were screened for further *** factors affecting PTB were analyzed by univariate analysis,and the related risk factors were identified by logistic *** According to univariate analysis,the PTB group was older than the FT group,with a smaller weight change and greater proportions of women who underwent artificial insemination and had gestational diabetes mellitus(Ps of women with reproductive tract infections and greater white blood cell(WBC)counts(Pshorter cervical lengths in the second trimester and lower neutrophil percentages(Pstpartum sAs and sDs scores than did the FT group(Ps experiencing anxiety and depress

关键词： Preterm birth Risk factors Postpartum psychological state Risk model Prediction

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Effect of nursing on postoperative respiratory function and mental health of lung cancer patients

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World Journal of Clinical Cases 2024年第5期12卷 922-930页

作者： Xiang Yang Dan Yin shi-Qing Chen Department of Rehabilitation The First People's Hospital of Jiangxia DistrictWuhan(Union Jiangnan Hospital Huazhong University of Science and Technology)Wuhan 430200Hubei ProvinceChina Department of Intensive Care The First People's Hospital of Jiangxia DistrictWuhan(Union Jiangnan Hospital Huazhong University of Science and Technology)Wuhan 430200Hubei ProvinceChina Department of Neurology The First People's Hospital of Jiangxia DistrictWuhan(Union Jiangnan Hospital Huazhong University of Science and Technology)Wuhan 430200Hubei ProvinceChina

BACKGROUND Both pulmonary rehabilitation training and psychological care have been shown to have a positive effect on the postoperative recovery of patients with lung ***,few studies have combined the two to explore their combined ***,this study aimed to investigate the effects of pulmonary rehabil-itation training combined with psychological care on postoperative respiratory function and mental health in lung cancer *** To investigate effect of nursing on postoperative respiratory function and mental health of lung cancer *** 122 cases of lung cancer patients who underwent surgical treatment in our hospital and were treated in our department from January 2022 to April 2023 were selected and randomly divided into the control group and observation *** control group performed the routine care *** obser-vation group was given pulmonary rehabilitation training and psychological care based on conventional nursing *** expiratory volume,forced vital *** ventilatory volume(MVV)in one second was measured,and the patient's 6-min walking distance and dyspnoea index scale were used to assess the patient's respiratory *** Connor-Davidson resilience scale(CD-RIsC),self-rating anxiety scale(sAs),and self-rating depression scale(sDs)were used to evaluate the mental health of the *** There was no difference between the two groups regarding age,gender,education level,surgical procedure,type of pathology,and treatment(P>0.05).After treatment,MVV,6-min walking distance,toughness,strength,optimism,and total CD-RIsC scores were significantly higher in the observation group(Pspnoea scores,sAs,and sDs scores were substantially lower in the control group compared to the observation group(PsION Pulmonary rehabilitation training combined with psychological care for patients after lung cancer resection could improve lung function,enhance daily activities,e

关键词： Pulmonary rehabilitation training Psychological care Lung cancer Postoperative care Respiratory function

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Prognostic value of PEA3 subfamily gene expression in cholangiocarcinoma

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World Journal of Gastrointestinal Oncology 2024年第9期16卷 4014-4027页

作者： Li Wang Zhe Zhang Hai-Zhang Ma Department of Emergency The Second Affiliated Hospital of Zhejiang University School of MedicineHangzhou 310000Zhejiang ProvinceChina Department of Emergency Medicine The First People’s Hospital of Linping District HangzhouHangzhou 311100Zhejiang ProvinceChina Department of General surgery Qilu Hospital of Shandong UniversityJinan 250000Shandong ProvinceChina

BACKGROUND Cholangiocarcinoma(CCA)is a lethal malignancy with limited treatment options and poor *** PEA3 subfamily of E26 transformation specific genes:ETV1,ETV4,and ETV5 are known to play significant roles in various cancers by influencing cell proliferation,invasion,and *** To analyze PEA3 subfamily gene expression levels in CCA and their correlation with clinical parameters to determine their prognostic value for *** The expression levels of PEA3 subfamily genes in pan-cancer and CCA data in the cancer genome atlas and genotype-tissue expression project databases were analyzed with R language *** curve and receiver operating characteristic analyses were performed using the survMiner,survival,and Procr language *** gene expression profiling interactive analysis 2.0 database was used to analyze the expression levels of PEA3 subfamily genes in different subtypes and stages of *** Gestalt was used to perform the gene ontology/Kyoto encyclopedia of genes and genomes(GO/KEGG)analysis,and sTRING database analysis was used to determine the genes and proteins related to PEA3 subfamily *** ETV1,ETV4,and ETV5 expression levels were significantly increased in *** were significant differences in ETV1,ETV4,and ETV5 expression levels among the different subtypes of CCA,and predictive analysis revealed that only high ETV1 and ETV4 expression levels were significantly associated with shorter overall survival in patients with ***/KEGG analysis revealed that PEA3 subfamily genes were closely related to transcriptional misregulation in *** vitro and in vivo experiments revealed that PEA3 silencing inhibited the invasion and metastasis of CCA *** The expression level of ETV4 may be a predictive biomarker of survival in patients with CCA.

关键词： PEA3 subfamily Cholangiocarcinoma The cancer genome atlas ETV4 The prognosis Prognostic biomarker

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Residual inflammatory risk predicts long-term outcomes following stenting for symptomatic intracranial atherosclerotic stenosis

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stroke & Vascular Neurology 2024年第4期9卷 407-417页

作者： Ying Yu Yake Lou Yuesong Pan Long Yan Weilun Fu Zhikai Hou Rongrong Cui Zhongrong Miao Yongjun Wang Xin Lou Ning Ma Department of Interventional Neuroradiology Beijing Tiantan HospitalCapital Medical UniversityBeijingChina China National Clinical Research Center for Neurological Diseases Beijing Tiantan HospitalBeijingChina Department of Neurology Beijing Tiantan HospitalCapital Medical UniversityBeijingChina Department of Cardiology The Second Affiliated Hospital of Chongqing Medical UniversityChongqingChina Department of Neurology Beijing Daxing District People's HospitalBeijingChina Radiology Chinese PLA General HospitalBeijingChina

Background and purpose Residual inflammatory risk(RIR)can predict the unfavourable outcomes in patients with minor ischaemic ***,the impact of preprocedural RIR on long-term outcomes in patients with symptomatic intracranial atherosclerotic stenosis(sICAs)who underwent stenting remains *** This retrospective,single-centre cohort study evaluated consecutive patients with severe sICAs who underwent intracranial *** were categorised into four groups based on preprocedural high-sensitivity C-reactive protein(hs-CRP)and low-density lipoprotein cholesterol(LDL-C):residual cholesterol inflammatory risk(RCIR,hs-CRP≥3 mg/L and LDL-C≥2.6 mmol/L),RIR(hs-CRP≥3 mg/L and LDL-Csidual cholesterol risk(RCR,hs-CRPsidual risk(NRR,hs-CRPs included recurrent ischaemic stroke and *** long-term imaging outcomes consisted of in-stent restenosis(IsR)and symptomatic IsR(sIsR)after *** In this study,952 patients were included,with 751(78.9%)being ***-six cases were categorised into the RCIR group,211 into the RIR group,107 into the RCR group and 588 into the NRR *** with RCIR(adjusted HR 6.163;95%CI 2.603 to 14.589;psted HR 2.205;95%CI 1.294 to 3.757;p=0.004)had higher risks of recurrent ischaemic stroke than those with NRR during the 54 months of median follow-up *** with RCIR(adjusted HR 3.604;95%CI 1.431 to 9.072;p=0.007)were more likely to occur IsR,and patients in the RIR group showed a significant increase in the risk of sIsR(adjusted HR 2.402;95%CI 1.078 to 5.351;p=0.032)compared with those in the NRR group with a median follow-up time of 11.9 *** In patients with sICAs,preprocedural RIR may predict long-term recurrent ischaemic stroke,IsR and sIsR following intracranial stenting.

关键词： intracranial stenosis symptomatic

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Crohn’s disease as the intestinal manifestation of pan-lymphatic dysfunction:An exploratory proposal based on basic and clinical data

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World Journal of Gastroenterology 2024年第1期30卷 34-49页

作者： Yu-Wei Zhou Yue Ren Miao-Miao Lu Ling-Ling Xu Wei-Xin Cheng Meng-Meng Zhang Lin-Ping Ding Dong Chen Jian-Guo Gao Juan Du Ci-Liang Jin Chun-Xiao Chen Yun-Fei Li Tao Cheng Peng-Lei Jiang Yi-Da Yang Peng-Xu Qian Peng-Fei Xu Xi Jin Department of Gastroenterology The First Affiliated Hospital of Zhejiang University School of MedicineHangzhou 310003Zhejiang ProvinceChina Department of Gastroenterology The Second Hospital of JiaxingJiaxing 314000Zhejiang ProvinceChina Endoscopy Center Children’s Hospital of Zhejiang University School of MedicineHangzhou 310003Zhejiang ProvinceChina Department of Gastroenterology The Second People’s Hospital of Yuhang DistrictHangzhou 310000Zhejiang ProvinceChina Department of Gastroenterology Hangzhou Shangcheng District People’s HospitalHangzhou 310003Zhejiang ProvinceChina Department of Colorectal surgery The First Affiliated Hospital of Zhejiang University School of MedicineHangzhou 310003Zhejiang ProvinceChina Department of Gastroenterology The First Affiliated Hospital of Wenzhou Medical UniversityWenzhou 325000Zhejiang ProvinceChina Women’s hospital and Institute of Genetics Zhejiang University School of MedicineHangzhou 310000Zhejiang ProvinceChina Center of stem Cell and Regenerative Medicine and Bone Marrow Transplantation CenterThe First Affiliated Hospital of Zhejiang University School of MedicineHangzhou 310003Zhejiang ProvinceChina Department of Infectious Disease The First Affiliated Hospital of Zhejiang University School of MedicineHangzhou 310003Zhejiang ProvinceChina

Crohn’s disease(CD)is caused by immune,environmental,and genetic *** can involve the entire gastrointestinal tract,and although its prevalence is rapidly increasing its etiology remains *** biological and small-molecule drugs have advanced the treatment of CD;however,a considerable proportion of patients are non-responsive to all known *** achieve a breakthrough in this field,innovations that could guide the further development of effective therapies are of utmost *** this review,we first propose the innovative concept of pan-lymphatic dysfunction for the general distribution of lymphatic dysfunction in various diseases,and suggest that CD is the intestinal manifestation of pan-lymphatic dysfunction based on basic and clinical preliminary *** supporting evidence is fully summarized,including the existence of lymphatic system dysfunction,recognition of the inside-out model,disorders of immune cells,changes in cell plasticity,partial overlap of the underlying mechanisms,and common gut-derived fatty and bile acid *** benefit of this novel concept is that it proposes adopting the zebrafish model for studying intestinal diseases,especially CD,as this model is good at presenting and mimicking lymphatic *** importantly,the ensuing focus on improving lymphatic function may lead to novel and promising therapeutic strategies for CD.

关键词： Inflammatory bowel disease Crohn’s disease Lymphatic system Inside-out model Immune cells Zebrafish

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Promise and challenges of traditional Chinese medicine,specifically Calculus bovis,in liver cancer treatment

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World Journal of Gastroenterology 2024年第40期30卷 4380-4385页

作者： Ao-Xi Xu Zhi-Feng Zhao Li Zhu Yi-Heng Zhang Yan Li Yu-Fan Wei Bo-Ya Zhang Bin Jiang Tian-Ze Gao Meng-si Li Jia-Yu Liu school of Medicine Nankai UniversityTianjin 300071China Department of General surgery The First Medical Center of Chinese People's Liberation Army General HospitalBeijing 100853China Medical school of Chinese people's Liberation Army Medical School of Chinese People's Liberation ArmyBeijing 100853China Department of Hepatobiliary and Pancreative surgery The First Medical CenterChinese People's Liberation Army General HospitalBeijing 100853China Department of surgery Mancheng District People's HospitalBaoding 072150Hebei ProvinceChina Department of Neurosurgery First Medical Centre of Chinese People's Liberation Army General HospitalBeijing 100853China

Liver cancer,one of the most common malignancies worldwide,ranks sixth in incidence and third in *** cancer treatment options are diverse,inclu-ding surgical resection,liver transplantation,percutaneous ablation,transarterial chemoembolization,radiotherapy,chemotherapy,targeted therapy,immuno-therapy,and traditional Chinese medicine(TCM).A multidisciplinary team(MDT)is essential to customize treatment plans based on tumor staging,liver function,and performance status(Ps),ensuring individualized patient *** decisions require a MDT to tailor strategies based on tumor staging,liver function,and Ps,ensuring personalized *** approval of new first-line and second-line drugs and the establishment of standard treatments based on immune checkpoint inhibitors have significantly expanded treatment options for advanced liver cancer,improving overall ***,many patients do not respond effectively to these treatments and ultimately succumb to the *** oncology treatments,while extending patient survival,often come with severe side effects,resistance,and damage to the body,negatively impacting quality of *** et al's study published at World Journal of Gastroenterology rigorously validates the anticancer properties of Calculus bovis,enhancing our understanding of TCM and contributing to new liver cancer treatment *** over 5000 years,TCM has been used in East Asian countries like China to treat various diseases,including liver *** of real-world clinical data suggests that for patients with advanced-stage tumors lacking effective treatments,integrated TCM therapies could provide significant breakthroughs.

关键词： Traditional Chinese medicine Liver cancer Calculus bovis Treatment Promise Challenges

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Comparative study of 3D-Printed Porous Titanium Alloy with Rod Designs of Three Different Geometric structures for Orthopaedic Implantation

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Acta Metallurgica sinica(English Letters) 2024年第1期37卷 54-66页

作者： Jiaxin Li Haozhang Zhong Bojun Cao Zhaoyang Ran Jia Tan Liang Deng Yongqiang Hao Jinglong Yan Department of Orthopedics The Second Affiliated Hospital of Harbin Medical UniversityHarbin150001China shanghai Engineering Research Center of Innovative Orthopaedic Instruments and Personalized Medicine Shanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineShanghai200011China Institute of Materials Modification and Modeling Shanghai Jiao Tong UniversityShanghai200240China

Porous titanium alloy is currently widely used in clinical treatment of orthopaedic diseases for its lower elastic modulus and ability to integrate with bone *** the micro-level,cells can respond to different geometries,and at the macro-level,the geometric design of implants will also affect the biological function of *** this study,three kinds of porous scaffolds with square,triangular and circle rod shapes were designed and 3D *** study observed the proliferation and differentiation of MC3T3-E1 cells during surface culture of the three types of *** also evaluated the characteristics of the three scaffolds by means of compression tests and scanning electron microscopy to provide a reference for the design of porous titanium alloy implants for clinical *** trends of cell proliferation and gene expression between the three types of scaffolds were observed after treatment with two *** results show that the square rod porous scaffolds have the best proliferative and osteogenic activities,and these findings may be due to differences in piezo-type mechanosensitive ion channel component 1(Piezo1)and Yes-associated protein(YAP)expression caused by the macro-geometric topography.

关键词： Porous titanium scaffolds Geometric morphology Cell proliferation structural design

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Multidimensional Analgesia of Acupuncture by Increasing Expression of MD2 in Central Nervous system

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Chinese Journal of Integrative Medicine 2024年第11期30卷 1035-1044页

作者： LI Wan-rong REN Lu-lu ZHAO Tian-tian DAI Dan-qing GAO Xiao-fei LIANG Hua-zheng XIONG Li-ze shanghai Key Laboratory of Anesthesiology and Brain Functional Modulation Shanghai Fourth People's Hospital School of Medicine Tongji University Clinical Research Center for Anesthesiology and Perioperative Medicine Shanghai Fourth People's Hospital School of Medicine Tongji University Translational Research Institute of Brain and Brain-Like Intelligence Shanghai Fourth People's Hospital School of Medicine Tongji University Department of Anesthesiology and Perioperative Medicine Shanghai Fourth People's Hospital School of Medicine Tongji University school of Rehabilitation Southwest Jiaotong University Monash suzhou Research Institute

Objective:To investigate changes of myeloid differentiation factor 2（MD2） in inflammationinduced pain and acupuncture-mediated ***:Mice were randomly divided into three groups by a random number table method:saline group（n=16）,complete Freund's adjuvant（CFA） group（n=24） and CFA+electroacupuncture（EA） group（n=26）.Inflammation-induced pain was modelled by injecting CFA to the plantar surface of the hind paw of mice and EA was applied to bilateral Zusanli（sT 36） to alleviate *** mice in the CFA+EA group received EA treatment（30 min/d for 2 weeks） 24 h after *** in the saline and CFA groups received sham ***-Frey test and Hargreaves test were used to assess the pain *** and spinal tissues were collected for immunofluorescence staining or Western blotting to quantify changes of MD2 ***:CFA successfully induced plantar pain and EA significantly alleviated pain 3 days after modelling（Ps+neurons was significantly increased in the dorsal horn of the spinal cord 7 and 14 days after EA,especially in laminae Ⅰ-Ⅱ_o（Psitive cells to the number of c-fos positive cells and the mean fluorescence intensity of MD2 neurons were also significantly increased in laminae Ⅰ-Ⅱ_o（Pstern blotting showed that the level of MD2 was significantly decreased by EA only in the hippocampus on day 7 and 14（both Psignificant changes were observed in the cortex,thalamus,cerebellum,or the brainstem（P>0.05）.Fluorescence staining showed significant decrease in the level of MD2 in periagueductal gray（PAG） and locus coeruleus（LC） after CFA injection on day 7（Psignificantly reversed this decrease（Psion:The unique changes of MD2 suggest that EA may exert the analgesic effect through modulating neuronal activities of the superficial laminae of the spinal cord and certai

关键词： dorsal horn electroacupuncture inflammation-induced pain locus coeruleus myeloid differentiation factor 2 periaqueductal gray

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