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Chemical Composition, Proapoptotic and Antiosteoporosis Activities of the Essential oil from the Aerial Part of <i>Alpinia officinarum</i>Hance

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Chinese Medicine 2018年第3期9卷 144-164页

作者： Yang Wang Ji Chen Keda Zhang Weigang Wu Lanlan Ge Haoqiang Wan Jiemei Li Jun Tian Xiaobin Zeng Key Lab for New Drug Research of TCM and Shenzhen Branch State R&D Centre for Viro-Biotech Research Institute of Tsinghua University in Shenzhen Shenzhen China Center Lab of Longhua Branch Shenzhen People’s Hospital Second Clinical Medical College of Jinan University Shenzhen China Shenzhen Hanyu Pharmaceutical Co. Ltd Shenzhen China Department of Orthopedics Shenzhen People’s Hospital 2nd Clinical Medical College of Jinan University Shenzhen China Department of Infectious Disease Shenzhen People’s Hospital 2nd Clinical Medical College of Jinan University Shenzhen China College of Life Science Jiangsu Normal University Xuzhou China Guangdong Key Laboratory for Research and Development of Natural Drugs Guangdong Medical University Zhanjiang China

Background: Alpinia officinarum Hance is valued as an edible medicinal plant. The rhizome is widely reported to have anticancer activity whereas little information is available on the aerial part. This study investigates chemical composition, proapoptotic and anti-osteoporosis activities of essential oil from aerial parts of A. officinarum (APEO). Methods: In this study, APEO was extracted by hydrodistillation and analyzed using GC-MS. The inhibitive activity of 0 - 2.5 μL/mL. APEO was investigated using MTT assay, while in vivo effect was evaluated in nude mice. The cell cycle, apoptosis, Δψm and expression of proteins analyses influenced by 0 - 0.313 μL/mL APEO were detected by PI, Annexin V/PI, JC-1, and Western blot, respectively. Alkaline phosphatase activity and mineralized nodules formation of rat osteoblasts with 0 - 0.156 μL/mL APEO were assayed using colorimetric method and alizarin red staining, respectively. Results: Total 45 constituents were identified accounting for 91.1% of APEO (sesquiterpene hydrocarbons for 44.4%). APEO significantly inhibited cancer cells growth in a dose-dependent manner. APEO also inhibited cancer growth in vivo. The percentage of S phase cells is up to 64.846% with 0.313 μL/mL APEO. The proportion of total apoptotic cells significantly increased to 79.6% at 0.313 μL/mL concentration. APEO treated cells accompanied with Bcl-2 and Δψm decrease, and caspase-3 and p53 upregulation. Furthermore, addition of APEO in rat osteoblasts led to a dose-dependent increase in ALP activity and formation of mineralized bone nodules. Conclusions: Our data suggest that APEO could be developed as an agent against human lung cancer and osteoporosis, especially cancer-induced bone loss.

关键词： Alpinia officinarum Hance Essential oil Cancer Apoptosis Anti-Osteoporosis

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Chemical composition and antimicrobial activities of essential oil extracted from Tithonia diversifolia(Asteraceae)flower

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Journal of Bioresources and Bioproducts 2016年第4期1卷 169-176页

作者： Oludare O.Agboola Stephen Oyedeji Joshua O Olowoyo Abdulwakeel Ajao Oladipo Aregbesola Department of Biology Sefako Makgatho Health Sciences UniversityPretoriaSouth Africa. Department of Botany University of LagosAkokaYabaLagos StateNigeria. Department of plant Biology University of IlorinIlorin240003 Nigeria. Department of Botany Obafemi Awolowo UniversityIle-IfeOsun StateNigeria Department of Microbiology Obafemi Awolowo UniversityIle-IfeOsun StateNigeria

The upsurge in the prevalence of side effects of many synthetic antimicrobial agents and incidence of multidrug resistant bacteria has spurred scientists on the search for plant based antimicrobial of therapeutic *** study extracted essential oils from the flowers of Tithonia diversifolia(Hemsl)***(Mexican sunflower)and assayed it for antimicrobial *** oils were extracted(hydro-distillation),characterized(GC-MS)and tested for antibacterial and antifungal activities.α-Pinene(34.42%),β-Caryophyllene(22.34%),β-Pinene(11.14%),Germacrene-D(11.13%)and 1,8-Cineole(8.76%)were the major constituents of the forty-five compounds *** characterized compounds were general monoterpenes(44.44%),sesquiterpenes(26.67%),including alcohols and aldehydes which accounted for 28.89%.The extract concentrations of 8-120 mg/ml in Potato Dextrose Agar(PDA)medium effectively inhibited the tested fungi in *** 5 mg/ml,only Bacillus megaterium and Bacillus cereus were inhibited of all gram-positive bacteria while Streptococcus pyrogens was *** gram-negative bacteria were *** inhibition of the gram-positive and gram-negative species increases with increased concentration of the essential *** 40 mg/ml,all the tested bacteria species were inhibited and the growth inhibition for the species followed the order;***>Proteus mirabi>Bacillus megaterium>Klebsiella pneumonia>Bacillus cereus>*** oil concentration of 72 mg/ml totally inhibited the growth of all three fungal species-Cochliobolus lunatus,Fusarium solani,Fusarium *** secondary metabolites in the oil of *** proved effective against the tested bacteria and fungi *** finding of this study unlocks the potentials of this essential oil of Mexican sunflower for bio-pesticide production.

关键词： Essential oils Flower Gas chromatography Monoterpenes Sesquiterpenes

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