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Breakthro.gh o. interspecific chimerism:blo.king apo.to.is

Cell Regeneratio. 2021年第1期10卷 212-214页

作者： Yiren Qin Duancheng Wen ronald o.perelman and claudia cohen center fo. reproductive medicine Weill Cornell MedicineNew YorkNY 10065USA

o. January 28 in Nature o.line, Zheng et al. (Nature, 2021 (o.line ahead o. print)) repo.ted that they develo.ed aningenio.s metho. o. interspecies PSC co.culture system in vitro.which unfo.ded interspecific cell co.petitio.. Thisstudy paves the way fo. disco.ering the mechanism o. interspecific chimera and fo. further interspecifico.gano.enesis between evo.utio.arily distant species.

关键词： culture Nature

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A mo.ified pro.o.o. fo. <i>in vitro.lt;/i>maturatio. o. mo.se o.cytes fro. seco.dary preantral fo.licles

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Advances in Bio.cience and Bio.echno.o.y 2012年第1期3卷 57-74页

作者： Weido.g Wang Yaxu Tang Lujuan Ni Theerayut Jo.gwutiwes Hung-Ching Liu Zev Ro.enwaks the ronald o. perelman and claudia cohen center for reproductive medicine Weill Medical College of Cornell University New York USA

A 2-step culture system was designed and tested fo. the in vitro.maturatio. efficiency o. o.cytes fro. pre-puberty preantral fo.licles o. FVB/N inbred mice. The fo.lo.ing mo.ificatio.s were made: 1) The co.centratio. o. ITS was reduced by half in the basal MIF medium to.minimize unco.rdinated gro.th between o.cyte and GC cells;2) Hetero.eneo.s preantral fo.licles were cultured in gro.ps o. 3 - 5 fo.licles in hanging dro.s o. medium with reduced co.centratio. o. ITS fo. six days to.inductio. fo.licular aggregatio.. This hanging dro. metho. mimics a 3-D IVM culture system at the early stage o. cultivatio. in which the sphere structure o. each fo.licle is well maintained. It also.enables fo.licles in each aggregate to.co.municate with each o.her, synchro.ize their gro.th, and thus prevent immature fo.licular rupture. 3) Medium was further supplemented with retino.c acid to.enhance develo.mental capacity o. meio.ically arrested o.cytes. After a 14-day culture in vitro. ~37% o. the co.lected inbred preantral fo.licles co.pleted nuclear maturatio.. Appro.imately 94% o. the mature o.cytes tested were able to.be fertilized;and 77% o. them develo.ed into.healthy embryo.. These results demo.strate that o.r IVM system is reliable to.pro.uce a satisfacto.y number o. high quality o.cytes. In additio., multiple cyto.lasmic parameters, including gene expressio. o. key regulato.s, chro.o.o.e/spindle o.ganizatio., mito.ho.drial pro.iferatio. and distributio., and to.al ATP co.tent were explo.ed to.characterize the suppo.tive and limiting co.po.ents o. o.r IVM system so.that the culture system can be further o.timized.

关键词： Fo.licle o.cyte In Vitro Maturatio. In Vitro Fertilizatio. Hanging Dro.s Fertility Spindle Mito.ho.drio. and ATP Co.tent

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Dual ro.e o. lipids fo. geno.e stability and pluripo.ency facilitates full po.ency o. mo.se embryo.ic stem cells

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蛋白质与细胞:英文版 2023年第8期14卷 591-602页

作者： Liangwen Zho.g Miriam Go.dillo.Xingyi Wang Yiren Qin Yuanyuan Huang Alexey So.hnev Ritu Kumar Go.ri Nanjangud Daylo. James C.David Allis To.d Evans Bryce Carey Duancheng Wen Department o. reproductive medicine Ronald O.Perelman and Claudia Cohen Center for Reproductive MedicineWeill Cornell MedicineNew YorkNY 10065USA Department o. Surgery Weill Cornell Medical College1300 York AvenueNew YorkNY 10065USA Labo.ato.y o. Chro.atin Bio.o.y and Epigenetics The Rockefeller UniversityNew YorkNY 10065USA Co.d Spring Harbo. Labo.ato.y Cold Spring HarborNY 11724USA Mo.ecular Cyto.enetics Co.e.Memo.ial Slo.n Kettering Cancer center New YorkNY 10065USA Department o. Neuro.cience Developmental and Regenerative BiologyUniversity of Texas at San AntonioOne UTSA CircleSan AntonioTX 78249USA Gladsto.e Institutes 1650 Owens StSan FranciscoCA94158USA

While Mek1/2 and Gsk3βinhibitio.("2i")suppo.ts the maintenance o. murine embryo.ic stem cells(EsCs)in a ho.o.eno.s naive state,pro.o.ged culture in 2i results in aneuplo.dy and DNA hypo.ethylatio. that impairs develo.mental ***,2i fails to.suppo.t derivatio. and culture o. fully po.ent female *** we find that mo.se ESCs cultured in 2i/LIF supplemented with lipid-rich albumin(AlbuMAx)undergo.pluripo.ency transitio. yet maintain geno.ic stability and full po.ency o.er lo.g-term ***,lipids in AlbuMAx impact intracellular metabo.ism including nucleo.ide bio.ynthesis,lipid bio.enesis,and TCA cycle intermediates,with enhanced expressio. o. DNMT3s that prevent DNA *** induce a fo.mative-like pluripo.ent state thro.gh direct stimulatio. o. Erk2 pho.pho.ylatio.,which also.alleviates X chro.o.o.e lo.s in female ***,bo.h male and female"all-ESc"mice can be generated fro. de no.o.derived ESCs using AlbuMAXbased *** findings undersco.e the impo.tance o. lipids to.pluripo.ency and link nutrient cues to.geno.e integrity in early develo.ment.

关键词： mo.se pluripo.ent stem cells lipids pluripo.ency transitio. geno.ic stability develo.mental po.ency nucleo.ide po.l depletio. 2i medium X chro.o.o.e lo.s femaleall-EsC mice

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