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Gene-modified leucoconcentrate for personalized ex vivo gene therapy in a mini pig model of moderate spinal cord injury

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Neural Regeneration Research 2021年第2期16卷 357-361页

作者： Rustem R.Islamov Farid V.Bashirov Mikhail E.Sokolov Andrei A.Izmailov Filip O.Fadeev Vage A.Markosyan Maria A.Davleeva Olga V.Zubkova Maxim M.Smarov Denis Yu.Logunov Boris S.Naroditskyi Ilnur I.Salafutdinov Albert A.Rizvanov Ramil G.Turaev Kazan State Medical University KazanRussia Gamaleya Research Institute of Epidemiology and Microbiology MoscowRussia Kazan Federal University KazanRussia the republican blood center of the ministry of health of the republic of tatarstan KazanRussia

We previously demonstrated that gene-modified umbilical cord blood mononuclear cells overexpressing a combination of recombinant neurotrophic factors are a promising therapeutic approach for cell-mediated gene therapy for neurodegenerative diseases,neurotrauma,and *** this study,using a mini pig model of spinal cord injury,we proposed for the first time the use of gene-modified leucoconcentrate prepared from peripheral blood in the plastic blood bag for personalized ex vivo gene *** obtained from mini pig peripheral blood was transduced with a chimeric adenoviral vector(Ad5/35 F)that carried an enhanced green fluorescent protein(EGFP)reporter gene in the plastic blood *** day after blood donation,the mini pigs were subjected to moderate SCI and four hours post-surgery they were intravenously autoinfused with gene-modified leucoconcentrate.A week after gene-modified leucoconcentrate therapy,fluorescent microscopy revealed EGFP-expressing leucocytes in spinal cord at the site of contusion *** the spleen the groups of EGFP-positive cells located in the lymphoid follicles were *** vitro flow cytometry and fluorescent microscopy studies of the gene-modified leucoconcentrate samples also confirmed the production of EGFP by ***,the efficacy of leucocytes transduction in the plastic blood bag and their migratory potential suggest their use for temporary production of recombinant biologically active molecules to correct certain pathological *** paper presents a proof-of-concept of simple,safe and effective approach for personalized ex vivo gene therapy based on gene-modified leucoconcentrate *** animal protocols were approved by the Kazan State Medical University Animal Care and Use Committee(approval No.5)on May 27,2014.

关键词： chimeric Ad5/35F virus enhanced green fluorescent protein gene-modified leucoconcentrate mini pig peripheral blood personalized ex vivo gene therapy plastic blood bag spinal cord injury

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Features of Cellular,Humoral and Activation Markers of Immune System and Biophysical Parameters of Superficial Membranes of Lymphocytes of Periphery blood at Patients with a Breast Cancer

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海外英语 2010年第4X期 202-207页

作者： Alimhodzhaeva L. T. The republican Research center of Oncology under the ministry of health of the republic of Uzbekistan Tashkent

Studied features of cellular and humoral factors of immune system and activation markers of lymphocytes at breast cancer *** biophysical parameters of lymphocyte superficial membranes of periphery blood of breast cancer *** of immune system reaction were:expressed Т - cellular immunodeficiency,deficiency of CD4+Т-helpers/inducers against raised expression of СD8+Т-lymphocyte,suppression of immunoregulatory index,imbalance of immunity humoral link,activity of expression CD16 + and CD56 + on natural killers and rising of expression CD38 + and CD95 + on *** of biophysical parameters of lymphocytes superficial membranes has allowed to reveal statistically significant changes characterized by intensifying of electric field and rising ofmicroviscosity,obviously leading to disappearance or weakening of intercellular interactions.

关键词： cellular immunity humoral immunity innovation markers of lymphocytes microviscosity of membranes a superficial charge of membranes of lymphocytes breast cancer.

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The Diagnostic Value of the Investigation of the Proinflammatory and Anti-inflammatory Cytokines Levels in the Immune System of Patients with Breast Cancer

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海外英语 2010年第4X期 208-211页

作者： L. T. Alimhojaeva The republican Research center of Oncology under the ministry of health of the republic of Uzbekistan Tashkent

Diagnostic value of serumal levels of proinflammatory(TNF-α,IL-1β,IL-6 and IFN-γ) and anti-inflammatory(IL-4 and IL-10) cytokines of immune system is investigated at 54 mammary glands sick by a *** analysis carried out has allowed to tap the raised concentration of such proinflammatory cytokines,as IL-1β,TNF-α and IL-6 in serum of peripheric blood of MGC *** analysis of antiinflammatory cytokines in Serum of peripheric blood of MGC patients also has taped authentically raised value of IL-4 and *** obtained data testify to presence imbalance cellular and humoral factors of the immune system which studying will serve as the important diagnostic criterion of breast cancer.

关键词： proinflammatory and antiinflammatory cytokines immunity immunopatogenezis breast cancer

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Luminal androgen receptor(LAR)subtype of triple-negative breast cancer:molecular,morphological,and clinical features

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2022年第8期23卷 617-624页

作者： Sergey VTORUSHIN Anastasia DULESOVA Nadezhda KRAKHMAL Department of Pathology Siberian State Medical University Ministry of Health of RussiaTomsk 634050Russia Department of General and Molecular Pathology Cancer Research InstituteTomsk National Research Medical Center of the Russian Academy of SciencesTomsk 634009Russia Department of Pathology Republican Clinical Oncological Dispensary Ministry of HealthTatarstan RepublicKazan 420029Russia

According to the classification presented by Lehmann BD(2016),triple-negative breast cancer(TNBC)is a heterogeneous group of malignant tumors with four specific subtypes:basal-like(subtype 1 and subtype 2),mesenchymal,and luminal androgen receptor(LAR)*** basal-like subtypes of carcinomas predominate in this group,accounting for up to 80%of all *** the significantly lower proportions of mesenchymal and LAR variants in the group of breast carcinomas with a TNBC profile,such tumors are characterized by aggressive biological *** this end,the LAR subtype is of particular interest,since the literature on such tumors presents different and even contradictory data concerning the disease course and *** review is devoted to the analysis of the relevant literature,reflecting the main results of studies on the molecular properties and clinical features of the disease course of LAR-type TNBC carcinomas.

关键词： Luminal androgen receptor(LAR)subtype Triple-negative breast cancer(TNBC) Androgen receptor(AR) Prognosis

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IgG Anti-Body Titers among Patients with Amoebic Liver Abscess in Bangui, Central African republic

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Open Journal of Immunology 2022年第4期12卷 77-85页

作者： Wilfrid Sylvain Nambei Edwige Régina Kodia-Lenguetama Junior Nguerenam-Ouefio Department of Biomedical Sciences Faculty of Health Sciences Bangui Central African Republic (CAR) National center of blood Donors’ Ministry of Public Health Bangui Central African Republic (CAR)

Entamoeba histolytica (Eh) is a protozoan parasite that causes amoebiasis characterized by intestinal damage and amoebic liver development and is an important cause of hospitalization in low-middle income countries. The aims of this study were to determine the prevalence and the titer of IgG anti Eh in ALA (Amoebic Liver Abscess) patients’ in Bangui according sex, age and other risk factors. This was a cross sectional study where data was collected between January 2018 and October 2020. The diagnosis of ALA was suspected based on clinical symptoms of;fever, abdominal pain (usually in the right hypochondrium or epigastrium) and clinical signs of hepatomegaly and/or tender liver with or without jaundice and abdominal ultra-sonography. ALA patients’ IgG antibody titers were measured by Indirect Hemmaglutination Assay and Chi-square test was used. A total of 1249 ALA patients were included, among whom 570 (45.64%) were positive. Of these, 244 (42.08%) had titer 1:160 or less, 223 patients’ (39.13%) had titer ranging from 1:320 to 1:640 and 103 patients (18.07%) had strong titer ranging from 1:1280 to 1:2560. The association between antibody titer results, age and sex was no significant (p = 1.0000). Our findings indicate a high prevalence of ALA and show no significant difference between the sex and age ALA patients.

关键词： Amoebic Liver Abscess Antibody Sex Difference Central African republic

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Detection of piroplasms infection in sheep, dogs and hedgehogs in Central China

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Infectious Diseases of Poverty 2014年第1期3卷 167-173页

作者： Zhuo Chen Qin Liu Feng-ChaoJiao Bian-LiXu Xiao-NongZhou National Institute of Parasitic Diseases Chinese Center for Disease Control and PreventionShanghai 200025People’s Republic of China WHO Collaborative center for Malaria Schistosomiasis and FilariasisKey Laboratory of Parasite and Vector BiologyMinistry of HealthShanghai 200025People’s Republic of China Xinyang College of Agriculture and Forestry Xinyang 464000People’s Republic of China Henan center for Disease Control and Prevention Zhengzhou 450016People’s Republic of China

Background:Piroplasms are kinds of tick-borne parasitic apicomplexan protozoa,which are detrimental to humans and animals in tropical and subtropical areas around the *** until now,there has been a limited amount of reliable information available about the prevalence of piroplasms infections in wild animals in ***,we have investigated the infections of Babesia and Theileria species in both domestic and wild animals in Xinyang city,Henan province,where tick-borne diseases have recently been *** study aims to analyze the distribution patterns of piroplasms infections in animals,and assess their potential threat to humans in Central ***:blood samples were collected from sheep,dogs and hedgehogs in two regions,including Shihe District and Luoshan County,of Xinyang city,Henan province from August to December *** *** Theileria *** detected by polymerase chain reaction(PCR)and identified by sequencing and phylogenetic ***,the characteristics of detected piroplasms in different animal hosts were compared between the two study ***:A total of 227 blood samples were collected from 73 sheep,two dogs and 152 *** *** only detected in the two *** *** detected both in the sheep and the hedgehogs,and the total positive rate of Theileria *** the sheep and the hedgehogs was 57.53%and 13.82%,*** and phylogenetic analysis revealed that the Theileria *** in the sheep and the hedgehogs were very close to *** cloned from a small ruminant and Theileria *** from a febrile hospitalized patient in ***:Babesia and Theileria infections were detected in both domestic and wild animals in Xinyang city,Henan province in Central China,thus warranting further studies in these regions.

关键词： Babesia spp Theileria spp Dogs Sheep Hedgehogs China

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GATA binding protein 2 mediated ankyrin repeat domain containing 26 high expression in myeloid-derived cell lines

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World Journal of Stem Cells 2024年第5期16卷 538-550页

作者： Yang-Zhou Jiang Lan-Yue Hu Mao-Shan Chen Xiao-Jie Wang Cheng-Ning Tan Pei-Pei Xue Teng Yu Xiao-Yan He Li-Xin Xiang Yan-Ni Xiao Xiao-Liang Li Qian Ran Zhong-Jun Li Li Chen Laboratory of Radiation Biology Department of Blood TransfusionLaboratory Medicine CenterThe Second Affiliated HospitalThird Military Medical UniversityChongqing 400037China Hematopoietic Acute Radiation Syndrome Medical and Pharmaceutical Basic Research Innovation center Ministry of Education of the People’s Republic of ChinaChongqing 400037China

BACKGROUND Thrombocytopenia 2,an autosomal dominant inherited disease characterized by moderate thrombocytopenia,predisposition to myeloid malignancies and normal platelet size and function,can be caused by 5’-untranslated region(UTR)point mutations in ankyrin repeat domain containing 26(ANKRD26).Runt related transcription factor 1(RUNX1)and friend leukemia integration 1(FLI1)have been identified as negative regulators of ***,the positive regulators of ANKRD26 are still *** To prove the positive regulatory effect of GATA binding protein 2(GATA2)on ANKRD26 *** Human induced pluripotent stem cells derived from bone marrow(hiPSC-BM)INTRODUCTION Ankyrin repeat domain containing protein 26(ANKRD26)acts as a regulator of adipogenesis and is involved in the regulation of feeding behavior[1-3].The ANKRD26 gene is located on chromosome 10 and shares regions of homology with the primate-specific gene family *** to the Human Protein Atlas database,the ANKRD26 protein is localized to the Golgi apparatus and vesicles,and its expression can be detected in nearly all human tissues[4].Moreover,UniProt annotation revealed that ANKRD26 is localized in the centrosome and contains coiled-coil domains formed by spectrin helices and ankyrin repeats[5,6].The most common disease related to ANKRD26 is thrombocytopenia 2(THC2),which is a rare autosomal dominant inherited disease characterized by lifelong mild-to-moderate thrombocytopenia and mild bleeding[7-9].Caused by the variants in the 5’-untranslated region(UTR)of ANKRD26,THC2 is defined by a decrease in the number of platelets in circulating blood and results in increased bleeding and decreased clotting ability[8,10].Due to the point mutations that occur in the 5’-UTR of ANKRD26,its negative transcription factors(TFs),Runt related transcription factor 1(RUNX1)and friend leukemia integration 1(FLI1),lose their repression effect[11].The persistent expression of ANKRD26 increases the activi

关键词： Ankyrin repeat domain containing 26 GATA binding protein 2 Thrombocytopenia 2 Transcriptional regulation Myeloid-derived cell lines

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Rubella Immunity among Pregnant Women in Bangui, Central African republic

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Open Journal of Immunology 2022年第4期12卷 130-136页

作者： Wilfrid Sylvain Nambei Junior Nguerenam-Ouefio Arsène Gbamonza Edwige Régine Kodia-Lenguetama Department of Biomedical Sciences Faculty of Health Sciences University of Bangui Bangui Central African Republic Communautaire Hospital of Bangui Bangui Central African Republic National center of blood Donors’ Ministry of Public Health Bangui Central African Republic

Sero-positivity rates of the rubella virus among pregnant women vary from country to country widely throughout the world. In the Central African republic, rubella vaccination is not included in the national immunization schedule. Thus, we propose to evaluate the immune status of pregnant women. This was an analytical retrospective study that consulted the records of pregnant women received in prenatal consultations at the Bangui Community Hospital maternity ward from January to December 2020. Socio-demographic and laboratory data (IgM, IgG) were collected from January to June 2021. Chi2 test was used. A total of 289 pregnant women were analyzed. Women with an IgM+ response accounted for 4.15%. Those with an IgG+ were 14.87%. The distribution by age group shows that patients aged 20 - 24 and those aged 25 - 29 had a rubella profile suggesting persistent infection (p = 0.010). The average age of women included was 28 (±6) years. The average parity for the entire sample was 2.18 (1.93). At any age pregnant women were not significantly exposed to rubella infection (p = 0.96), (ORbrut = 1.03;CI95% = [0.32 - 3.34]). Both immunized and non-immunized individuals are similarly exposed [OR = 0.86;95% IC95% = 0.44 - 1.68] with no difference (p = 0.96). Rubella infection remains particularly severe when it occurs during pregnancy. It would be wise to seek immunity in all girls of childbearing age in order to rule out any risk of rubella embryopathy.

关键词： Rubella Immunity Pregnant Women Central African republic

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Antibiotic Resistance Profile of Serotypes of Streptococcus pneumoniae Strains in Bangui, from 2017 to 2022: Case of Serotype 1

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Open Journal of Medical Microbiology 2024年第2期14卷 131-145页

作者： Zéphirin Dalengat Vogbia Ernest Lango Yaya Marceline Djeintote Stéphanie Judith N’Yetobouko Jean de Dieu Longo Clotaire Donatien Rafaï Christian Diamant Mossoro-Kpindet Gérard Gresenguet Epidemiology and Research Service National Reference Center for Sexually Transmitted Infections and Anti-Retroviral Therapy Ministry of Health Bangui Central African Republic Faculty of health Sciences Doctoral School of Human and Veterinary Health Sciences University of Bangui Bangui Central African Republic National Laboratory of Clinical Biology and Public health Bangui Central African Republic National blood Transfusion center Bangui Central African Republic Public Department Ministry of Health Bangui Central African Republic

Goals: The aim of this study was to determine the antibiotic resistance profile of serotypes of Streptococcus pneumoniae strains circulating in Bangui. Methodology: A prospective and analytical analysis was carried out at the National Laboratory of Clinical Biology and Public health from 2017 to 2022. The strains came from our study on the contribution to the study of antibiotic sensitivity of Streptococcus pneumoniae strains. The multiplex PCR test was used for its cost-effectiveness in terms of amplifiers which can be purified in order to be sequenced. It also makes it possible to detect several germs as well as their serotypes. For a PCR reaction, several elements are involved in the reaction medium or Master Mix. These are the desoxyribonucleotides (dNTPs), the magnesium ions (MgCl2) and the primers. A set of 14 primers divided into 3 classes were used. Class 1 primers served as an internal control by targeting the cpsA gene. It is a highly conserved gene found in capsular loci characterized to date. The primers of the second class were used to target specific serotypes by specific reactions (out of six possibilities). The group reaction was carried out using the primers of the third class in order to carry out an initial screening of the samples and to classify the pneumococcal isolates. Related serotypes were grouped based on the amplification of common genes. Using the technique of electrophoresis on agarose gel and an ultraviolet radiation device, the migration bands are then visualized and analyzed. The data collected had been entered into Excel 2010 and analyzed with Epi info 7. The exact Fischer chi2 test at the 5% threshold, the relative risk and its 95% confidence interval were used to compare the proportions and determine the associations. Results: 187 antibiotic-resistant strains of Streptococcus pneumoniae were collected. The average frequency of serotypes 1, 9A, 4 and untypeable identified were 43.59%, 18.18%, 18.27% and 39.57% respectively. The fre

关键词： Streptococcus pneumonia Serotype Antibiotic Resistance Bangui

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Humoral Response to Toxoplasma gondii in Pregnant Women in Bangui Central African republic

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Open Journal of Immunology 2022年第4期12卷 122-129页

作者： Wilfrid Sylvain Nambei Junior Ouefio Nguerenam Edwige Régina Kodia-Lenguetama Arsène Gbamonza Department of Science Biomedical Faculty of Health Science University of Bangui Bangui Central African Republic National center of blood Donor’s Ministry of Public Health Bangui Central African Republic Community Hospital of Bangui Bangui Central African Republic

Toxoplasmosis is a cosmopolitan antrhropozoonosis widespread in mammals and birds. Normally asymptomatic in the subject health, it can have serious consequences for the fetus in the first trimester of pregnancy in the pregnant woman. It is in this context that we propose to assess the immune response to T. gondii in pregnant women in Bangui. This was a retrospective analytical study that consulted the records of pregnant women received in prenatal consultations at the Bangui Community Hospital Maternity ward from January 2019 to December 2019. Socio-demographic and laboratory data (IgM, IgG response to T. gondii) and results of HIV serology were collected from January to June 2021. Chi2 test was used. A total of 307 pregnant women were analyzed. The average age of the women included was 28 (±6) years. The average parity of the entire sample was 2.18 (±1.93). Toxoplasmosis infectious was 14.65%. Women with a positive IgM response accounted for 17.58% and those with an IgG-positive response for 42.99%. Patients with a positive HIV were 5.86%. Patients aged 20 - 29 had a serological profile suggesting a probable ongoing infection (p = 0.010). The paucipares were more represented with no statistically significant difference (p = 0.23). Pregnant women were not significantly exposed to toxoplasmosis infectious (p = 0.96). Immunized and non-immunized subjects were similarly exposed [OR = 0.97;CI 95% 0.4 = 6 - 2.05]. Toxoplasmosis remains particularly serious during pregnancy. Seroprevalence was significantly higher in the 20 - 24 year age group. Women were similarly exposed depending on whether they were immunized or not. This requires the establishment of a specific prevention program against this disease.

关键词： T. gondii Humoral Response HIV Pregnant Women Central African republic

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