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Targeting CXCR4 and EDN1 for the treatment of recurrent miscarriage using stearic acid from traditional Chinese medicine

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Traditional Medicine Research 2024年第11期9卷 65-75页

作者： Fang Liu Dong-Mei Shi Wen-Ye Ma Da-Wei Tang Gang Bai Xin-You Yu Reproductive Center of Yinchuan Maternal and Child Health Hospital Yinchuan 750001China prenatal diagnosis Center of Ningxia medical University General Hospital Yinchuan 750004China

Background:Recurrent miscarriage(RM)affects an estimated 1-3%of couples attempting to conceive,and its molecular components stay ineffectively caught *** study aims to explore potential therapeutic targets for RM by examining gene expression patterns and biological pathways in both mouse and human RM ***,explore relevant traditional Chinese medicine(TCM)components targeting potential therapeutic ***:We utilized the GSE211251 mouse and the GSE26787 human datasets,employing gene set enrichment analysis and gene metaphysics analysis to examine differentially expressed genes and enriched ***-cell RNA analysis uncovered cellular heterogeneity and arranged pharmacology-mapped potential drug-target *** employed molecular docking strategies to assess the affinity of TCM components for key ***:In the mouse model,genes such as Ly6f1 and Gpr26 were upregulated,while Stc5a and Galca exhibited *** set enrichment analysis identified key pathways,including the tumor necrosis factor-mediated signaling *** human samples,Gene Ontology analysis highlighted processes such as apoptosis and cell ***-cell RNA analysis revealed distinct cellular populations between normal and RM *** pharmacology identified C-X-C motif chemokine receptor 4(CXCR4)and endothelin 1(EDN1)as potential key targets,and molecular docking confirmed that stearic acid from TCM appears to regulate these ***:This study presents a comprehensive analysis of the genetic and cellular underpinnings of RM,identifying CXCR4 and EDN1 as promising therapeutic *** acid from TCM could provide targeted treatment by modulating these key proteins,paving the way for new RM treatment strategies.

关键词： RM gene expression single-cell RNA analysis CXCR4 EDN1 stearic acid

Role of lysosomal trafficking regulator in autophagic lysosome reformation in neurons:a disease perspective

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Neural Regeneration Research 2024年第5期19卷 957-958页

作者： Prashant Sharma Jenny Serra-Vinardell Wendy J.Introne May Christine V.Malicdan NIH Undiagnosed Diseases program Common FundOffice of the DirectorNational Institutes of HealthBethesdaMDUSA Human Biochemical genetics Section Medical Genetics BranchNational Human Genome Research InstituteNational Institutes of HealthBethesdaMDUSA(Serra-Vinardell JIntrone)

Lysosomes are discrete organelles that act as recycling centers for extracellular and intracellular materials,playing a pivotal role in maintaining cellular *** acidic environment,maintained by numerous hydrolytic enzymes,facilitates substrate *** in lysosomal processes can lead to abnormal substrate degradation,significantly impacting cellular *** energy-demanding cells,such as post-mitotic neurons,are especially vulnerable to these changes,often resulting in neurological ***,a conserved catabolic process,requires extensive lysosomal *** plays a key role in removing unnecessary intracellular components,ensuring cellular homeostasis,and promoting cell survival during stress conditions such as starvation,infection,or cellular damage.

关键词： homeostasis lysosomal removing

MAD2L2 overexpression attenuates the effects of TNF-α-induced migration and invasion capabilities in colorectal cancer cells

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BIOCELL 2024年第9期48卷 1311-1322页

作者： HAOTONG SUN HEYING WANG YANJIE HAO XIN LI JUN LING HUAN WANG FEIMIAO WANG FANG XU School of Basic Medicine Medical Genetics and Cell BiologyNingxia Medical UniversityYinchuan750004China Key Laboratory of Reproduction and genetics Ningxia Medical UniversityYinchuan750004China

Background:Colorectal cancer is a major global health concern,exacerbated by tumor necrosis factor-alpha(TNF-α)and its role in inflammation,with the effects of Mitotic Arrest Deficient 2 Like 2(MAD2L2)in this context still ***:The colorectal carcinoma cell lines HCT116 and SW620 were exposed to TNF-αfor a period of 24 h to instigate an inflammatory *** assessments were conducted to measure the expression of inflammatory cytokines,the activity within the p38 mitogen-activated protein kinase(p38 MAPK)and Phosphoinositide 3-Kinase/AKT Serine/Threonine Kinase pathway(PI3K/AKT)signaling *** sequencing and subsequent integrative analysis with the Cancer Genome Atlas(TCGA)program database revealed a significant downregulation of the key factor *** of MAD2L2 expression was facilitated via lentiviral vector-mediated *** influence of this overexpression on TNF-α-prompted inflammation,intracellular signaling pathways,and the migratory and invasive behaviors of the colorectal cancer cells was then ***:TNF-αtreatment significantly increased the expression of Interleukin-1 beta(IL-1β)and Interleukin-6(IL-6),activated the MAPK p38 and PI3K/AKT signaling pathways,and enhanced cell migration and invasion.A decrease in MAD2L2 expression was observed following TNF-α***,overexpression of MAD2L2 reversed the effects of TNF-α,reducing IL-1βand IL-6 levels,attenuating PI3K/AKT pathway activation,and inhibiting cell migration and ***:Overexpression of MAD2L2 attenuates the pro-inflammatory effects of TNF-α,suggesting that MAD2L2 plays a protective role against TNF-α-induced migration and invasion of colorectal carcinoma ***,MAD2L2 holds potential as a therapeutic target in the treatment of colorectal cancer.

关键词： Colorectal cancer TNF-α MAD2L2 Migration Invasion

Resident immune responses to spinal cord injury:role of astrocytes and microglia

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Neural Regeneration Research 2024年第8期19卷 1678-1685页

作者： Sydney Brockie Cindy Zhou Michael G.Fehlings Division of genetics and Development Krembil Research InstituteUniversity Health NetworkTorontoONCanada Institute of medical Science University of TorontoTorontoONCanada Division of Neurosurgery and Spine program Department of SurgeryUniversity of TorontoTorontoONCanada

Spinal cord injury can be traumatic or non-traumatic in origin,with the latter rising in incidence and prevalence with the aging demographics of our *** r,as the global population ages,individuals with co-existent degenerative spinal pathology comprise a growing number of traumatic spinal cord injury cases,especially involving the cervical spinal *** makes recovery and treatment approaches particula rly challenging as age and comorbidities may limit regenerative *** these reasons,it is critical to better understand the complex milieu of spinal cord injury lesion pathobiology and the ensuing inflammatory *** review discusses microglia-specific purinergic and cytokine signaling pathways,as well as microglial modulation of synaptic stability and plasticity after ***,we evaluate the role of astrocytes in neurotransmission and calcium signaling,as well as their border-forming response to neural *** the inflammatory and reparative roles of these cells have eluded our complete understanding and remain key therapeutic targets due to their extensive structural and functional roles in the nervous *** advances have shed light on the roles of glia in neurotransmission and reparative injury responses that will change how interventions are *** key processes and existing knowledge gaps will allow future research to effectively target these cells and harness their regenerative potential.

关键词： astrocytes glial signaling microglia spinal cord injury synaptic transmission

Brassinosteroid biosynthesis and signaling:Conserved and diversified functions of core genes across multiple plant species

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Plant Communications 2024年第9期5卷 72-100页

作者： Brian Zebosi Erik Vollbrecht Norman B.Best Department of genetics Development and Cell BiologyIowa State UniversityAmesIA 50011USA Interdepartmental genetics and Genomics Graduate program Iowa State UniversityAmesIA 50011USA USDA-ARS Plant Genetics Research UnitColumbiaMO 65201USA

Brassinosteroids(BRs)are important regulators that control myriad aspects of plant growth and development,including biotic and abiotic stress responses,such that modulating BR homeostasis and signaling presents abundant opportunities for plant breeding and crop *** and other proteins involved in the biosynthesis and signaling of BRs are well understood from molecular genetics and phenotypic analysis in Arabidopsis thaliana;however,knowledge of the molecular functions of these genes in other plant species,especially cereal crop plants,is *** this manuscript,we comprehensively review functional studies of BR genes in Arabidopsis,maize,rice,Setaria,Brachypodium,and soybean to identify conserved and diversified functions across plant species and to highlight cases for which additional research is in *** performed phylogenetic analysis of gene families involved in the biosynthesis and signaling of BRs and re-analyzed publicly available transcriptomic *** trees coupled with expression data provide a valuable guide to supplement future research on BRs in these important crop species,enabling researchers to identify gene-editing targets for BR-related functional studies.

关键词： brassinosteroids maize rice Setaria soybean Brachypodium Arabidopsis

Effect of male HBV infection on the outcomes of IVF/ICSI cycles:a retrospective cohort study based on propensity score matching

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Asian Journal of Andrology 2024年第4期26卷 415-420页

作者： Su-Qin Zhu Xiu-Hua Liao Wen-Wen Jiang Yan Sun Hui-Ling Xu Xiao-Jing Chen Bei-Hong Zheng Fujian Provincial Reproductive Medicine Center Fujian Maternity and Child Health HospitalCollege of Clinical Medicine for Obstetrics and Gynecology and PediatricsFujian Medical UniversityFuzhou 350001China Fujian Key Laboratory of prenatal diagnosis and Birth Defect Fuzhou 350001China Fujian Maternal-Fetal Clinical Medicine Research Center Fuzhou 350001China.

This study aimed to investigate the effects of male hepatitis B virus(HBV)infection on male fertility,embryonic development,and in vitro fertilization/intracytoplasmic sperm injection(IVF/ICSI)*** performed a retrospective cohort study that included 3965 infertile couples who received fresh embryo transfer cycles for the first time at the Fujian Maternity and Child Health Hospital(Fuzhou,China)from January 2018 to January *** couples were categorized based on their HBV infection status into the HBV group(HBV-positive men and HBV-negative women)and the control group(HBV-negative couples).A 1:1 propensity score matching was performed with relatively balanced *** characteristics,semen parameters,laboratory outcomes,clinical outcomes,and obstetric and neonatal outcomes were compared between *** propensity score matching,821 couples were included in each *** groups had similar semen parameters and obstetric and neonatal *** HBV group showed a significantly lower live birth rate than the control group(P<0.05).The HBV group had a significantly higher abortion rate than the control group(P<0.05).The rates of high-quality embryos and blastocyst formation were significantly lower in the HBV group than those in the control group(both P<0.05).In conclusion,in couples who undergo IVF/ICSI,male HBV infection reduces the live birth rate and increases the risk of ***,the incidence of low birth weight in women with IVF/ICSI does not increase with male HBV infection.

关键词： hepatitis B virus in vitro fertilization intracytoplasmic sperm injection live birth rate propensity score matching

Polygalacturonase-inhibiting proteins as an exogenously applied natural solution for prevention of postharvest fungal infections

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Synthetic and Systems Biotechnology 2024年第3期9卷 481-493页

作者： Tiffany Chiu Yanran Li Graduate program in genetics Genomicsand Bioinformatics1140 Batchelor HallUniversity of California RiversideCalifornia92521USA program of Chemical Engineering Department of NanoengineeringUniversity of CaliforniaSan DiegoCA92521USA

Polygalacturonase inhibiting proteins(PGIPs)are plant proteins involved in the inhibition of polygalacturonases(PGs),cell-wall degrading enzymes often secreted by phytopathogenic ***,we confirmed that PGIP2 from Phaseolus vulgaris(PvPGIP2)can inhibit the growth of Aspergillus niger and Botrytis cinerea on agar *** this study,we further validated the feasibility of using PGIP as an environmental and ecological friendly agent to prevent fungal infection *** found that application of either purified PGIP(full length PvPGIP2 or truncated tPvPGIP2_5-8),or PGIP-secreting Saccharomyces cerevisiae strains can effectively inhibit fungal growth and necrotic lesions on tobacco *** also examined the effective amount and thermostability of PGIP when applied on plants.A concentration of 0.75 mg/mL or higher can significantly reduce the area of *** *** activity of full-length PvPGIPs is not affected after incubation at various temperatures ranging from􀀀20 to 42◦C for 24 h,while truncated tPvPGIP2_5-8 lost some efficacy after incubation at 42◦***,we have also examined the efficacy of PGIP on tomato *** the purified PvPGIP2 proteins were applied to tomato fruit inoculated with *** at a concentration of roughly 1.0 mg/mL,disease inci-dence and area of disease had reduced by more than half compared to the controls without PGIP *** study explores the potential of PGIPs as exogenously applied,eco-friendly fungal control agents on fruit and vegetables post-harvest.

关键词： PGIP Antifungal agent Pest control Postharvest treatment

Meiotic nuclear divisions 1 suppresses the proliferation and invasion of pancreatic cancer cells via regulating H2A.X variant histone

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BIOCELL 2024年第1期48卷 111-122页

作者： DONGQIN WANG YAN SHI ZHIQIANG WANG JING ZHANG LUYAO WANG HONGYU MA SHUHUA SHI XIAOFU LIAN HUA HUANG XIAOJING WANG CHAOQUN LIAN Anhui Province Key Laboratory of Clinical and Preclinical Research in Respiratory Disease Molecular Diagnosis CenterThe Department of Pulmonary Critical Care MedicineFirst Affiliated Hospital of Bengbu Medical CollegeBengbu233030China Department of genetics School of Life SciencesBengbu Medical CollegeBengbu233000China Department of Clinical Medicine Bengbu Medical CollegeBengbu233030China Research Center of Clinical Laboratory Science Bengbu Medical CollegeBengbu233030China

Introduction:Among all malignant tumors of the digestive system,pancreatic carcinoma exhibits the highest mortality ***,prevention and effective treatment are urgent issues that need to be ***:The study focused on meiotic nuclear divisions 1(MND1),integrating data from the Gene Expression Profiling Interactive Analysis(GEPIA)database with prognostic survival ***,experiments at cellular level were employed to demonstrate the effect of MND1 on the proliferation and migration of *** small-molecule inhibitor of MND1 was used to suppress the migration of PC cells by knocking down MND1 using small interfering RNA(siRNA)in Patu-8988 and Panc1 cell ***:The results of Cell Counting Kit-8 indicated that the suppression of MND1 resulted in a decrease in cell *** healing and Transwell assays revealed that MND1 knockdown reduced cell migration and *** cytometry revealed that inhibiting MND1 hindered the cell ***,MND1 could stimulate the proliferation,migration,and invasion of Patu-8988 and Panc1 cells by increasing the expression of ***,MND1 had a positive effect on H2AFX expression in PC *** MND1 expression suggests the low overall survival rate of individuals diagnosed with ***:These findings suggest that MND1 has the potential to be a gene with the ability to accurately diagnose and treat PC.

关键词： Pancreatic carcinoma MND1 H2AFX Cell cycle

Nrf2-mediated ferroptosis of spermatogenic cells involved in male reproductive toxicity induced by polystyrene nanoplastics in mice

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2024年第4期25卷 307-323页

作者： Xufeng FU Hang HAN Hong YANG Bo XU Wenjie DAI Ling LIU Tiantian HE Xing DU Xiuying PEI Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education School of Basic Medical SciencesNingxia Medical UniversityYinchuan 750004China Key Laboratory of Reproduction and genetics in Ningxia Ningxia Medical UniversityYinchuan 750004China

Microplastics(MPs)and nanoplastics(NPs)have become hazardous materials due to the massive amount of plastic waste and disposable masks,but their specific health effects remain *** this study,fluorescence-labeled polystyrene NPs(PS-NPs)were injected into the circulatory systems of mice to determine the distribution and potential toxic effects of NPs in ***,whole-body imaging found that PS-NPs accumulated in the testes of ***,the toxic effects of PS-NPs on the reproduction systems and the spermatocytes cell line of male mice,and their mechanisms,were *** oral exposure to PS-NPs,their spermatogenesis was affected and the spermatogenic cells were *** spermatocyte cell line GC-2 was exposed to PS-NPs and analyzed using RNA sequencing(RNA-seq)to determine the toxic mechanisms;a ferroptosis pathway was found after PS-NP *** phenomena and indicators of ferroptosis were then determined and verified by ferroptosis inhibitor ferrostatin-1(Fer-1),and it was also found that nuclear factor erythroid 2-related factor 2(Nrf2)played an important role in spermatogenic cell ferroptosis induced by ***,it was confirmed in vivo that this mechanism of Nrf2 played a protective role in PS-NPs-induced male reproductive *** study demonstrated that PS-NPs induce male reproductive dysfunction in mice by causing spermatogenic cell ferroptosis dependent on Nrf2.

关键词： Polvstyrene nanoplastics(PS-NPs) Reproductive toxicity Ferroptosis Nuclear factor ervthroid 2-related factor 2(Nrf2)