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Effects of hydrogen peroxide on in vitro infection of Cryptosporidium parvum

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Progress in Natural Science:Materials International 2001年第12期11卷 68-73页

作者：黄克和,杨世广,MarkC.HEALEY college of veterinary medicine Nanjing Agricultural university Nanjing 210095 China Department of Animal Dairy and veterinary Sciences Utah state university Utah 84322-5600 USA Department of Animal Dairy and veterinary Sciences Utah state university Utah 84322-5600 USA

The effect of hydrogen peroxide (H2O2) on Cryptosporidium parvum infection and the protective function of free radical scavengers were studied in cultured bovine fallopian tubes epithelial cells. H2O2 treatment at 500 μmol/L and 1 mmol/L significantly inhibited excystation of bleach-treated oocysts (p < 0.01) . At 48 h postinoculation, H2O2 at 500 and 750 μmol/L resulted in a significant (p < 0.01) decrease of C. parvum infection by 35 .77% and 58.16% respectively in the cultured cells, when compared with the untreated control. Surprisingly, the infection was significantly increased from 22.21% to 39.33% (p<0.05) with lower concentration of H202( 50 - 200 μmol/L) . The inhibitory and stimulatory effects of H2O2 treatment on C. parvum infection were, to a certain extent, abolished in the presence of reduced glutathione or mannitol. These observations indicate that reactive oxygen species, such as H2O2, may play an important role in C. parvum infection.

关键词： Cryptosporidium parvum hydrogen peroxide free radical scavengers excystation cell culture.

PROJECT MANAGEMENT:RECENT DEVELOPMENTS AND RESEARCH OPPORTUNITIES

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Journal of Systems Science and Systems Engineering 2012年第2期21卷 129-143页

作者： Nicholas G.HALL Department of Management Sciences Fisher college of Business The Ohio State UniversityUSA

This paper studies the business process known as project management. This process has exhibited a remarkable growth in business interest over the last 15 years, as demonstrated by a 1000% increase in membership in the Project Management Institute since 1996. This growth is largely attributable to the emergence of many new diverse business applications that can be successfully managed as projects. The new applications for project management include IT implementations, research and development, new product and service development, corporate change management, and software development. The characteristics of modem projects are typically very different from those of traditional projects such as construction and engineering, which necessitates the development of new project management techniques. We discuss these recent practical developments. The history of project management methodology is reviewed, from CPM and PERT to the influential modem directions of critical chain project management and agile methods. We identify one important application area for future methodological change as new product and service development. A list of specific research topics within project management is discussed. The conclusions suggest the existence of significant research opportunities within project management.

关键词： Project management overview recent practical developments opportunities for research

Adipose tissue lipolysis and remodeling during the transition period of dairy cows

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Journal of Animal Science and Biotechnology 2017年第4期8卷 787-798页

作者： G.Andres Contreras Clarissa Strieder-Barboza William Raphael Department of Large Animal Clinical Sciences College of Veterinary MedicineMichigan State UniversityEast LansingMI 48824USA

Elevated concentrations of plasma fatty acids in transition dairy cows are significantly associated with increased disease susceptibility and poor lactation performance. The main source of plasma fatty acids throughout the transition period is lipolysis from adipose tissue depots. During this time, plasma fatty acids serve as a source of calories mitigating the negative energy balance prompted by copious milk synthesis and limited dry matter *** research has demonstrated that lipolysis in the adipose organ is a complex process that includes not only the activation of lipolytic pathways in response to neural, hormonal, or paracrine stimuli, but also important changes in the structure and cellular distribution of the tissue in a process known as adipose tissue remodeling. This process involves an inflammatory response with immune cell migration, proliferation of the cellular components of the stromal vascular fraction, and changes in the extracellular matrix. This review summarizes current knowledge on lipolysis in dairy cattle, expands on the new field of adipose tissue remodeling, and discusses how these biological processes affect transition cow health and productivity.

关键词： Adipose tissue macrophages Adipose tissue remodeling Lipolysis Transition dairy cows

Nsp2 and GP5-M of Porcine Reproductive and Respiratory Syndrome Virus Contribute to Targets for Neutralizing Antibodies

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Virologica Sinica 2019年第6期34卷 631-640页

作者： Jia Su Lei Zhou Bicheng He Xinhui Zhang Xinna Ge Jun Han Xin Guo Hanchun Yang Key Laboratory of Animal Epidemiology of the Ministry of Agriculture College of Veterinary Medicine and State Key Laboratory of Agrobiotechnology China Agricultural University

Porcine reproductive and respiratory syndrome virus(PRRSV)is characterized by its genetic variation and limited cross protection among heterologous *** though several viral structural proteins have been regarded as inducers of neutralizing antibodies(NAs)against PRRSV,the mechanism underlying limited cross-neutralization among heterologous strains is still *** the present study,examinations of NA cross reaction between a highly pathogenic PRRSV(HP-PRRSV)strain,JXwn06,and a low pathogenic PRRSV(LP-PRRSV)strain,HB-1/3.9,were conducted with viral neutralization assays in MARC-145 *** of the JXwn06-hyperimmuned pigs’sera could neutralize HB-1/3.9 in vitro and vice *** address the genetic variation between these two viruses that are associated with limited crossneutralization,chimeric viruses with coding regions swapped between these two strains were *** neutralization assays indicated that variations in nonstructural protein 2(nsp2)and structural proteins together contribute to weak cross-neutralization activity between JXwn06 and HB-1/***,we substituted the nsp2-,glycoprotein2(GP2)-,GP3-,and GP4-coding regions together,or nsp2-,GP5-,and membrane(M)protein-coding regions simultaneously between these two viruses to construct chimeric viruses to test cross-neutralization reactivity with hyperimmunized sera induced by their parental *** results indicated that the swapped nsp2 and GP5-M viruses increased the neutralization reactivity with the donor strain antisera in MARC-145 *** together,these results show that variations in nsp2 and GP5-M correlate with the limited neutralization reactivity between the heterologous strains HP-PRRSV JXwn06 and LP-PRRSV HB-1/3.9.

关键词： Porcine reproductive and respiratory syndrome virus(PRRSV) Neutralizing antibody(NA) Non-structural protein 2(nsp2) Structural proteins(SPs)

Identification of Nonstructural Protein 8 as the N-Terminus of the RNA-Dependent RNA Polymerase of Porcine Reproductive and Respiratory Syndrome Virus

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Virologica Sinica 2018年第5期33卷 429-439页

作者： Yuanyuan Liu Yunhao Hu Yue Chai Liping Liu Jiangwei Song Shaochuan Zhou Jia Su Lei Zhou Xinna Ge Xin Guo Jun Han Hanchun Yang Key Laboratory of Animal Epidemiology of the Ministry of Agriculture College of Veterinary Medicine and State Key Laboratory of Agrobiotechnology China Agricultural University

Porcine reproductive and respiratory syndrome virus (PRRSV) is a member within the family Arteriviridae of the order Nidovirales. Replication of this positive-stranded RNA virus within the host cell involves expression of viral replicase proteins encoded by two ORFs, namely ORF1 a and ORF1 b. In particular, translation of ORF1 b depends on a-1-ribosomal frameshift strategy. Thus, nonstructural protein 9 (nsp9), the first protein within ORF1 b that specifies the function of the viral RNA-dependent RNA polymerase, is expressed as the C-terminal extension of nsp8, a small nsp that is encoded by ORF1 a. However, it has remained unclear whether the mature form of nsp9 in virus-infected cells still retains nsp8,addressing which is clearly critical to understand the biological function of nsp9. By taking advantage of specific antibodies to both nsp8 and nsp9, we report the following findings. (1) In infected cells, PRRSV nsp9 was identified as a major product with a size between 72 and 95 k Da (72–95 KDa form), which exhibited the similar mobility on the gel to the in vitro expressed nsp8–9 ORF1 b, but not the ORF1 b-coded portion (nsp9 ORF1 b). (2) The antibodies to nsp8, but not to nsp7 or nsp10, could detect a major product that had the similar mobility to the 72–95 KDa form of nsp9. Moreover, nsp9 could be co-immunoprecipitated by antibodies to nsp8, and vice versa. (3) Neither nsp4 nor nsp2 PLP2 was able to cleave nsp8–nsp9 in vitro. Together, our studies provide experimental evidence to suggest that nsp8 is an N-terminal extension of *** findings here paves way for further charactering the biological function of PRRSV nsp9.

关键词： Porcine reproductive and respiratory syndrome virus (PRRSV) Nsp8 Nsp9

转录因子IRF3充分活化在调节先天抗病毒免疫反应中的作用机制解析

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中国兽医科学 2024年第2期54卷 282-282页

作者： Wei-Wei Li Xu-Xu Fan Zi-Xiang Zhu Xue-Jing Cao Zhao-Yu Zhu Dan-Shi Pei Yi-Zhuo Wang Ji-Yan Zhang Yan-Yi Wang Hai-Xue Zheng state Key Laboratory for Animal Disease Control and Prevention College of Veterinary Medicine Lanzhou University Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Sciences Lanzhou China Key Laboratory of Special Pathogens and Biosafety Wuhan Institute of Virology Center for Biosafety Mega-Science Chinese Academy of Sciences Wuhan China Gansu Province Research Center for Basic Disciplines of Pathogen Biology Lanzhou China

翻译后修饰在调节Ⅰ型干扰素分泌过程中发挥关键作用。转录因子IRF3作为关键接头分子,其活性受到多种翻译后修饰的严格调控,包括磷酸化、de SUMOylation、泛素化和乙酰化等。目前,关于TBK1介导的IRF3磷酸化如何驱动IRF3完全活化的文献... 详细信息

翻译后修饰在调节Ⅰ型干扰素分泌过程中发挥关键作用。转录因子IRF3作为关键接头分子,其活性受到多种翻译后修饰的严格调控,包括磷酸化、de SUMOylation、泛素化和乙酰化等。目前,关于TBK1介导的IRF3磷酸化如何驱动IRF3完全活化的文献报道非常有限。因此,了解IRF3磷酸化和活化状态之间的关联性具有重要的生理学意义。

关键词： Ⅰ型干扰素生理学意义翻译后修饰转录因子活化状态分泌过程文献报道泛素化

Metabolism of minor isoforms of prion proteins Cytosolic prion protein and transmembrane prion protein

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Neural Regeneration Research 2013年第30期8卷 2868-2878页

作者： Zhiqi Song Deming Zhao Lifeng Yang state Key Laboratory for Agrobiotechnology National Animal Transmissible Spongiform Encephalopathy Laboratory College of Veterinary Medicine China Agricultural University

Transmissible spongiform encephalopathy or prion disease is triggered by the conversion from cellular prion protein to pathogenic prion protein. Growing evidence has concentrated on prion protein configuration changes and their correlation with prion disease transmissibility and patho- genicity. In vivo and in vitro studies have shown that several cytosolic forms of prion protein with specific topological structure can destroy intracellular stability and contribute to prion protein pathogenicity. In this study, the latest molecular chaperone system associated with endoplasmic re- ticulum-associated protein degradation, the endoplasmic reticulum resident protein quality-control system and the ubiquitination proteasome system, is outlined. The molecular chaperone system directly correlates with the prion protein degradation pathway. Understanding the molecular mechanisms will help provide a fascinating avenue for further investigations on prion disease treatment and prion protein-induced neurodegenerative diseases.

关键词： neural regeneration neurodegeneration prion protein cytosolic form of prion protein transmem-brane form of prion protein metabolism protein degeneration ubiquitination molecular chaperone molecular mechanism neuroregeneration

Synthesis and biological evaluation of novel 1,2,4-triazole containing 1,2,3-thiadiazole derivatives

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Chinese Chemical Letters 2013年第12期24卷 1134-1136页

作者： Yue-Dong Li Wu-Tao Mao Zhi-Jin Fan Juan-Juan Li Zhen Fang Xiao-Tian Ji Xue-Wen Hua Guang-Ning Zong Feng-Yun Li Chao-Lun Liu Jian-Hua Yu state Key Laboratory of Elemento-Organic Chemistry Nankai University Division of Hematology College of Medicine and School of Public HealthThe Ohio State University

A series of novel 1,2,4-triazoles containing 1,2,3-thiadiazole derivatives were designed and synthesized. Their structures were confirmed by melting points, IR, 1H NMR, and elemental analysis and ESI-MS or HRMS. Preliminary bioassays indicated that these compounds exhibited very good insecticidal activity against Aphis laburni at 100 μg/mL, with mortality no less than 95%. Compounds 6a, 6c, 6f, 61 showed higher curative activity against TMV and compound 6h showed a higher induction effects against TMV in vivo at 100 μg/mL. Collectively, our data demonstrate a new strategy for control of insects and viruses.

关键词： 1 2 3-Thiadiazole 1 2 4-Triazole Insecticidal activity Anti-TMV activity

光驱动Rh/InGaN_(1-x)O_(x)纳米组装体甲烷干重整制合成气

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Science Bulletin 2024年第10期69卷 1400-1409页

作者：李亦昕李景林俞天奇邱亮 Syed M.Najib Hasan 姚琳潘虎 Shamsul Arafin Sharif Md.Sadaf 朱磊周宝文 Key Laboratory for Power Machinery and Engineering of Ministry of Education Research Center for Renewable Synthetic FuelSchool of Mechanical EngineeringShanghai Jiao Tong UniversityShanghai 200240China China-UK Low Carbon college Shanghai Jiao Tong UniversityShanghai 201306China Centre Energie Matériaux et TélécommunicationsInstitut National de la Recherche Scientifique(INRS)-Universitédu QuébecVarennes J3X 1E4Canada Department of Electrical and Computer Engineering The Ohio State UniversityColumbusOH43210USA

Light-driven dry reforming of methane toward syngas presents a proper solution for alleviating climate change and for the sustainable supply of transportation fuels and ***,Rh/InGaN_(1-x)O_(x) nanowires supported by silicon wafer are explored as an ideal platform for loading Rh nanoparticles,thus assembling a new nanoarchitecture for this grand *** combination with the remarkable photothermal synergy,the O atoms in Rh/InGaN_(1-x)O_(x) can significantly lower the apparent activation energy of dry reforming of methane from 2.96 eV downward to 1.70 *** as-designed Rh/InGaN_(1-x)O_(x) NWs nanoarchitecture thus demonstrates a measurable syngas evolution rate of 180.9 mmol g_(cat)^(-1) h^(-1) with a marked selectivity of 96.3% under concentrated light illumination of 6 W cm^(-2).What is more,a high turnover number(TON)of 4182 mol syngas per mole Rh has been realized after six reuse cycles without obvious activity *** correlative 18O isotope labeling experiments,in-situ irradiated X-ray photoelectron spectroscopy(ISI-XPS)and in-situ diffuse reflectance Fourier transform infrared spectroscopy characterizations,as well as density functional theory calculations reveal that under light illumination,Rh/InGaN_(1-x)O_(x) NWs facilitate releasing^(*)CH_(3) and H^(+)from CH_(4) by holes,followed by H_(2) evolution from H^(+)reduction with ***,the O atoms in Rh/InGaN_(1-x)O_(x) can directly participate in CO generation by reacting with the ^(*)C species from CH_(4) dehydrogenation and contributes to the coke elimination,in concurrent formation of O *** resultant O vacancies are then replenished by CO_(2),showing an ideal chemical *** work presents a green strategy for syngas production via light-driven dry reforming of methane.

关键词： Dry reforming of methane Photo-thermal catalysis Rh/InGaN_(1-x)O_(x)nanowires