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Artemisia argyi extract subfraction exerts an antifungal effect against dermatophytes by disrupting mitochondrial morphology and function

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Chinese Journal of Natural Medicines 2024年第1期22卷 47-61页

作者： CHEN Le ZHU Yunyun GUO Chaowei GUO Yujie ZHAO Lu MIAO Yuhuan DU Hongzhi LIU Dahui College of Pharmacy Hubei University of Chinese MedicineWuhan 430065China national Resource Center for Chinese Materia Medica China Academy of Chinese Medical SciencesBeijing 100700China College of Pharmacy Nanjing University of Chinese MedicineNanjing 210023China

Artemisia argyi(***),a plant with a longstanding history as a raw material for traditional medicine and functional diets in Asia,has been used traditionally to bathe and soak feet for its disinfectant and itch-relieving *** its widespread use,scientific evidence validating the antifungal efficacy of *** water extract(AAWE)against dermatophytes,particularly Trichophyton rubrum,Trichophyton mentagrophytes,and Microsporum gypseum,remains *** study aimed to substantiate the scientific basis of the folkloric use of *** by evaluating the antifungal effects and the underlying molecular mechanisms of its active subfraction against *** results indicated that AAWE exhibited excellent antifungal effects against the three aforementioned dermatophyte *** subfraction AAWE6,isolated using D101 macroporous resin,emerged as the most potent *** minimum inhibitory concentrations(mics)of AAWE6 against ***,***,and *** were 312.5,312.5,and 625μg·mL−1,*** electron microscopy(TEM)results and assays of enzymes linked to cell wall integrity and cell membrane function indicated that AAWE6 could penetrate the external protective barrier of ***,creating breaches(“small holes”),and disrupt the internal mitochondrial structure(“granary”).Furthermore,transcriptome data,quantitative real-time PCR(RT-qPCR),and biochemical assays corroborated the severe disruption of mitochondrial function,evidenced by inhibited tricarboxylic acid(TCA)cycle and energy ***,chemical characterization and molecular docking analyses identified flavonoids,primarily eupatilin(131.16±4.52 mg·g^(−1))and jaceosidin(4.17±0.18 mg·g^(−1)),as the active components of *** conclusion,the subfraction AAWE6 from *** exerts antifungal effects against dermatophytes by disrupting mitochondrial morphology and *** research validates the traditional use of *** and provides scient

关键词： Artemisia argyi Dermatophyte Antifungal activity Mitochondrial structure Mitochondrial function Transcriptome analysis

Artemisia argyi extract subfraction exerts an antifungal effect against dermatophytes by disrupting mitochondrial morphology and function

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Chinese Journal of Natural Medicines 2024年第01期 47-61页

作者： CHEN Le ZHU Yunyun GUO Chaowei GUO Yujie ZHAO Lu MIAO Yuhuan DU Hongzhi LIU Dahui College of Pharmacy Hubei University of Chinese Medicine College of Pharmacy Nanjing University of Chinese Medicine national Resource Center for Chinese Materia Medica China Academy of Chinese Medical Sciences

Artemisia argyi（A. argyi）, a plant with a longstanding history as a raw material for traditional medicine and functional diets in Asia, has been used traditionally to bathe and soak feet for its disinfectant and itch-relieving properties. Despite its widespread use, scientific evidence validating the antifungal efficacy of A. argyi water extract（AAWE） against dermatophytes, particularly Trichophyton rubrum, Trichophyton mentagrophytes, and Microsporum gypseum, remains limited. This study aimed to substantiate the scientific basis of the folkloric use of A. argyi by evaluating the antifungal effects and the underlying molecular mechanisms of its active subfraction against dermatophytes. The results indicated that AAWE exhibited excellent antifungal effects against the three aforementioned dermatophyte species. The subfraction AAWE6, isolated using D101 macroporous resin, emerged as the most potent subfraction. The minimum inhibitory concentrations（mics） of AAWE6 against T. rubrum, M. gypseum, and T. mentagrophytes were 312.5,312.5, and 625 μg·m L-1, respectively. Transmission electron microscopy（TEM） results and assays of enzymes linked to cell wall integrity and cell membrane function indicated that AAWE6 could penetrate the external protective barrier of T. rubrum, creating breaches（“small holes”）, and disrupt the internal mitochondrial structure（“granary”）. Furthermore, transcriptome data, quantitative real-time PCR（RT-q PCR）, and biochemical assays corroborated the severe disruption of mitochondrial function, evidenced by inhibited tricarboxylic acid（TCA） cycle and energy metabolism. Additionally, chemical characterization and molecular docking analyses identified flavonoids, primarily eupatilin(131.16 ± 4.52 mg·g-1) and jaceosidin(4.17 ± 0.18 mg·g-1), as the active components of AAWE6. In conclusion, the subfraction AAWE6 from A. argyi exerts antifungal effects against dermatophytes by disrupting mitochondrial morphology and function. This research validates the

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ATPG is required for the accumulation and function of chloroplast ATP synthase in Arabidopsis

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Chinese Science Bulletin 2013年第26期58卷 3224-3232页

作者： KONG MengMeng WANG FenFei YANG ZhongNan MI HuaLing national Key Laboratory of plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences College of Life and Environmental Sciences Shanghai Normal University

The subunit Ⅱ of chloroplast ATP synthase is one of the two peripheral stalks, which associates the catalytic CF1 with mem-brane-spanning CFo . Although the structural and functional roles of chloroplast ATP synthase have been extensively examined, the physiological significance of subunit Ⅱ in vivo is still unclear. In this work, we identified one Arabidopsis T-DNA insertion mutant of atpG gene encoding the subunit Ⅱ of chloroplast ATP synthase. The atpg null mutant displayed an albino lethal pheno-type, as it could not grow photoautotrophically. Transmission electron microscopy analysis showed that chloroplasts of atpg lacked the organized thylakoid membranes. Loss of subunit Ⅱ affected the accumulation of CF1-CFo complex, however, it did not seem to have an effect on the CF1 assembly. The light induced ATP formation of atpg was significantly reduced compared with the wild type. Based on these results, we suggested that ATPG was essential for the accumulation and function of chloroplast ATP synthase.

关键词： ATP合酶 ATPG 叶绿体拟南芥积累透射电子显微镜分析 T-DNA插入类囊体膜

MVS-pheno:A Portable and Low-Cost phenotyping Platform for Maize Shoots Using Multiview Stereo 3D Reconstruction

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plant phenomics 2020年第1期2卷 40-56页

作者： Sheng Wu Weiliang Wen Yongjian Wang Jiangchuan Fan Chuanyu Wang Wenbo Gou Xinyu Guo Beijing Research Center for Information Technology in Agriculture Beijing 100097China national Engineering Research Center for Information Technology in Agriculture Beijing 100097China Beijing Key Lab of Digital plant Beijing 100097China

plant phenotyping technologies play important roles in plant research and *** phenotypes of individual plants can guide the optimization of shoot architecture for plant breeding and are useful to analyze the morphological differences in response to environments for crop ***,high-throughput phenotyping technologies for individual plants grown in field conditions are urgently needed,and MVS-pheno,a portable and low-cost phenotyping platform for individual plants,was *** platform is composed of four major components:a semiautomatic multiview stereo(MVS)image acquisition device,a data acquisition console,data processing and phenotype extraction software for maize shoots,and a data management *** platform’s device is detachable and adjustable according to the size of the target *** sequences for each maize shoot can be captured within 60-120 seconds,yielding 3D point clouds of shoots are reconstructed using MVS-based commercial software,and the phenotypic traits at the organ and individual plant levels are then extracted by the *** correlation coefficient(R^(2))between the extracted and manually measured plant height,leaf width,and leaf area values are 0.99,0.87,and 0.93,respectively.A data management system has also been developed to store and manage the acquired raw data,reconstructed point clouds,agronomic information,and resulting phenotypic *** platform offers an optional solution for high-throughput phenotyping of field-grown plants,which is especially useful for large populations or experiments across many different ecological regions.

关键词： breeding Stereo yielding

Development of a widely targeted volatilomics method for profiling volatilomes in plants

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Molecular plant 2022年第1期15卷 189-202页

作者： Honglun Yuan Guangping Cao Xiaodong Hou Menglan Huang Pengmeng Du Tingting Tan Youjin Zhang Haihong Zhou Xianqing Liu Ling Liu Yiding Jiangfang Yufei Li Zhenhuan Liu Chuanying Fang Liqing Zhao Alisdair R.Fernie Jie Luo College of Tropical Crops Hainan UniversityHaikou 570288China Sanya Nanfan Research Institute of Hainan University Hainan Yazhou Bay Seed LaboratorySanya572025China College of Chemistry and Environmental Engineering Shenzhen UniversityShenzhen 518060China national Key Laboratory of Crop Genetic Improvement and national Center of plant Gene Research(Wuhan) Huazhong Agricultural UniversityWuhan 430070China Department of Molecular Physiology Max-Planck-lnstitute of Molecular Plant PhysiologyAm Muhlenberg 114476 Potsdam-GolmGermany

Volatile organic compounds play essential roles in plant environment interactions as well as determining the fragrance of *** gas chromatography-mass spectrometry-based untargeted metabolo-mics is commonly used to assess plant volatiles,it suffers from high spectral convolution,low detection sensitivity,a limited number of annotated metabolites,and relatively poor ***,we report a widely targeted volatilomics(WTV)method that involves using a“targeted spectra extraction”algorithm to address spectral convolution,constructing a high-coverage MS2 spectral tag library to expand volatile annotation,adapting a multiple reaction monitoring mode to improve sensitivity,and using regression models to adjust for signal *** newly developed method was used to profile the volatilome of rice *** with the untargeted method,the newly developed WTV method shows higher sensitivity(for example,the signal-to-noise ratio of guaicol increased from 4.1 to 18.8),high annotation coverage(the number of annotated volatiles increased from 43 to 132),and better reproducibility(the number of volatiles in quality control samples with relative standard deviation value below 30.0%increased from 14 to 92 after normalization).Using the WTV method,we studied the metabolic responses of tomato to environmental stimuli and profiled the volatilomes of different rice *** results identified benzothiazole as a potential airborne signal priming tomato plants for enhanced defense and 2-nonanone and 2-heptanone as novel aromatic compounds contributing to rice *** case studies suggest that the widely targeted volatilomics method is more efficient than those currently used and may considerably promote plant volatilomics studies.

关键词： widely targeted volatilomics GC-MS MS2T library plant volatilome rice tomato

HYPOSENSITIVE TO LIGHT, an Alpha/Beta Fold Protein, Acts Downstream of ELONGATED HYPOCOTYL 5 to Regulate Seedling De-Etiolation

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Molecular plant 2011年第1期4卷 116-126页

作者： Xiao-Dong Sun Min Ni Department of plant Biology University of Minnesota St Paul MN 55108 USA

Ambient light has profound effects on early seedling de-etiolation through red and far-red light-absorbing phytochromes and blue and UV-A light-absorbing cryptochromes. Subsequent integration of various light signal trans- duction pathways leads to changes in gene expression and morphogenic responses. Here, we report the isolation of a new Arabidopsis light-signaling component, HYPOSENSITIVE TO LIGHT or HTL. Both htl-1 and htl-2 alleles displayed a long hypocotyl phenotype under red, far-red, and blue light, whereas overexpression of HTL caused a short hypocotyl pheno- type under similar light conditions. The mutants also showed other photomorphogenic defects such as elongated petioles, retarded cotyledon and leaf expansion, reduced accumulation of chlorophyll and anthocyanin pigments, and attenuated expression of light-responsive CHLOROPHYLL A/B BINDING PROTEIN 3 and CHALCONE SYNTHASE genes. HTL belongs to an alpha/beta fold protein family and is localized strongly in the nucleus and weakly in the cytosol. The expression of HTL was strongly induced by light of various wavelengths and this light induction was impaired in elongated hypocotyl 5. HY5 directly bound to both a C/G-box and a G-box in the HTL promoter but with a greater affinity toward the C/G-box. HTL, therefore, represents a new signaling step downstream of HY5 in phy- and cry-mediated de-etiolation responses.

关键词： HTL de-etiolation alpha/beta fold protein HY5.

Exploring the diversity of plant proteome

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Journal of Integrative plant Biology 2021年第7期63卷 1197-1210页

作者： Yanmei Chen Yi Wang Jun Yang Wenbin Zhou Shaojun Dai State Key Laboratory of plant Physiology and Biochemistry College of Biological SciencesChina Agricultural UniversityBeijing 100193China State Key Laboratory of Wheat and Maize Crop Science College of Resources and EnvironmentHenan Agricultural UniversityZhengzhou 450002China Shanghai Key Laboratory of plant Functional Genomics and Resources Shanghai Chenshan Plant Science Research CenterChinese Academy of SciencesShanghai Chenshan Botanical GardenShanghai 201602China Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing 100081China Development Center of plant Germplasm Resources College of Life SciencesShanghai Normal UniversityShanghai 200234China

The tremendous functional,spatial,and temporal diversity of the plant proteome is regulated by multiple factors that continuously modify protein abundance,modifications,interactions,localization,and activity to meet the dynamic needs of *** the proteome complexity and its underlying genetic variation is attracting increasing research *** spectrometry(MS)‐based proteomics has become a powerful approach in the global study of protein functions and their relation-ships on a systems ***,we review recent breakthroughs and strategies adopted to unravel the diversity of the proteome,with a specific focus on the methods used to analyze posttranslational modifications(PTMs),protein localization,and the organization of proteins into functional *** also consider PTM crosstalk and multiple PTMs temporally regulating the life cycle of ***-nally,we discuss recent quantitative studies using MS to measure protein turnover rates and examine future directions in the study of the plant proteome.

关键词： glycosylation mass spectrometry organellar proteo-mics phosphorylation SnRK kinase ubiquitination

plant responses to simulated warming and drought:a comparative study of functional plasticity between congeneric mid and high elevation species

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Journal of plant Ecology 2018年第3期11卷 364-374页

作者： Elena Hamann Halil Kesselring Jürg Stöcklin Botanical Institute Department of Environmental SciencesSection Plant EcologyUniversity of BaselSchönbeinstrasse 64056 BaselSwitzerland

Aims Effects of climate change,especially changes in temperatures and precipitation patterns,are particularly pronounced in alpine *** response,plants may exhibit phenotypic plasticity in key functional traits allowing short-term adjustment to novel ***,little is known about the degree of pheno-typic plasticity of high elevation species relative to mid elevation *** We transplanted 14 herbaceous perennial species from high eleva-tion into two common gardens(1050 and 2000 m.a.s.l.)in the Swiss Alps,and we examined plastic responses in key functional traits to changes in temperature and soil water *** design was replicated with 14 congeneric species from mid elevation to assess if the degree of phenotypic plasticity differs between mid and high elevation *** was assessed across two growing seasons,while aboveground biomass and specific leaf area(SLA)were measured after the first growing season,and biomass alloca-tion to belowground and reproductive structures after the ***,a phenotypic plasticity index was calculated for the func-tional traits to compare the degree of plasticity between mid and high elevation *** Findings Aboveground biomass was higher in mid elevation species relative to high elevation congeners in all treatments,yet decreased for both with elevation and ***,SLA decreased with elevation and *** mass fraction(RMF)was generally higher in high ele-vation species,and decreased with drought at the lower *** increased the allocation to reproductive structures,especially when plants were grown at their elevation of ***,no differ-ence was found in the degree of phenotypic plasticity averaged across mid and high elevation species for any of the studied functional *** results indicate that phenotypic plasticity in the focal traits did not depend on the elevation of origin of the *** was not related to

关键词： biomass allocation common garden climate change perennial herbaceous species phenotypic plasticity SLA Swiss Alps transplant experiment

Transcriptome-based Discovery of Fusarium graminearum Stress Responses to FgHV1 Infection

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Transcriptome-based Discovery of Fusarium graminearum Stress...