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The virulence regulator AbsR in avian pathogenic Escherichia coli has pleiotropic effects on bacterial physiology

Journal of Integrative Agriculture 2024年第2期23卷 649-668页

作者： Dongfang Zhao Haobo Zhang Xinyang Zhang Fengwei Jiang Yijing Li Wentong Cai Ganwu Li State Key laboratory for animal Disease Control and Prevention Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150069China College of Veterinary Medicine Northeast Agricultural UniversityHarbin 150038China National animal Tuberculosis Reference laboratory Division of Zoonoses SurveillanceChina Animal Health and Epidemiology CenterQingdao 266032China Department of Veterinary Diagnostic and Production animal Medicine College of Veterinary MedicineIowa State UniversityAmesIowa 50011-1134the United States

Avian pathogenic Escherichia coli(APEC)belonging to extraintestinal pathogenic E.coli(ExPEC)can cause severe infections in extraintestinal tissues in birds and humans,such as the lungs and blood.MprA(microcin production regulation,locus A,herein renamed AbsR,a blood survival regulator),a member of the MarR(multiple antibiotic resistance regulator)transcriptional regulator family,governs the expression of capsule biosynthetic genes in human ExPEC and represents a promising druggable target for antimicrobials.However,a deep understanding of the AbsR regulatory mechanism as well as its regulon is lacking.In this study,we present a systems-level analysis of the APEC AbsR regulon using ChIP-Seq(chromatin immunoprecipitation sequencing)and RNA-Seq(RNA sequencing)methods.We found that AbsR directly regulates 99 genes and indirectly regulates 667 genes.Furthermore,we showed that:1)AbsR contributes to antiphagocytotic effects by macrophages and virulence in a mouse model for systemic infection by directly activating the capsular gene cluster;2)AbsR positively impacts biofilm formation via direct regulation of the T2SS(type II secretion system)but plays a marginal role in virulence;and 3)AbsR directly upregulates the acid tolerance signaling system EvgAS to withstand acid stress but is dispensable in ExPEC virulence.Finally,our data indicate that the role of AbsR in virulence gene regulation is relatively conserved in ExPEC strains.Altogether,this study provides a comprehensive analysis of the AbsR regulon and regulatory mechanism,and our data suggest that AbsR likely influences virulence primarily through the control of capsule production.Interestingly,we found that AbsR severely represses the expression of the type I-F CRISPR(clustered regularly interspaced short palindromic repeats)-Cas(CRISPR associated)systems,which could have implications in CRISPR biology and application.

关键词： avian pathogenic Escherichia coli(APEC) extraintestinal pathogenic Escherichia coli(ExPEC) AbsR RNA-Seq ChIP-Seq gene regulation

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Transmission of ceftazidime-avibactam-resistant Escherichia coli among pets, veterinarians and animal hospital environment

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生物安全与健康(英文) 2024年第3期6卷 191-198页

作者： Dai Hegen Shao Dongyan Song Yu An Qi Zhang Zhenbiao Zhang Haixia Chen Siyu Wu Congming Shen Jianzhong Lyu Yanli Wang Yang Ma Shizhen Xia Zhaofei National Key laboratory of Veterinary Public Health Safety College of Veterinary Medicine China Agricultural University Beijing China Key laboratory of animal Antimicrobial Resistance surveillance Ministry of Agriculture and Rural Affairs College of Veterinary Medicine China Agricultural University Beijing China Department of Clinical Veterinary Medicine College of Veterinary Medicine China Agricultural University Beijing China Shanxi Key laboratory for Modernization of TCVM College of Veterinary Medicine Shanxi Agricultural University Taiyuan China Ruichen Pet Hospital Group Co. Ltd Xiamen China

Ceftazidime-avibactam (CZA) is a recently approved combination synthetic β-lactamase inhibitor used in human clinical medicine. Cases of CZA resistance in humans have already been reported, but limited research has investigated CZA resistance in pets. This study explored the prevalence and transmission of CZA-resistantEscherichia coli (CZAREC) among pets, their owners, veterinarians, and the environment in animal hospitals. A total of 5,419 clinical samples were collected from dogs and cats, along with samples from the environment (n = 5,843), veterinarians (n = 557), and pet owners (n = 368) in animal hospitals. From these samples, 760Escherichia coli (E. coli) isolates were obtained, out of which 60 were identified as CZAREC. These included 34 isolates from the environment (9.14 %, n = 372), three from veterinarians (8.11 %, n = 37), and 23 from animals (6.82 %, n = 337). No CZAREC isolates were found in pet owners. The predominant sequence types of CZARECs were ST156 (n = 20), ST410 (n = 19) and ST101 (n = 7). Bayesian analysis revealed six clusters comprising 47 isolates from the hospital environment, pets, and veterinaries, displaying genetic relatedness of less than 100 core genome single nucleotide polymorphisms (cgSNPs) between any two isolates in each cluster. Some CZAREC isolates with high genetic similarity persisted in the same animal hospital for four to six months. Moreover, discriminant analysis of principal components indicated that most isolates from different hosts shared a genetic source in the human/dog/cat merged cluster. Overall, evidence of CZARECs transmission was found among pets, the environment, and veterinarians in animal hospitals. The findings emphasize the importance of monitoring CZARECs in the veterinary clinical setting to ensure the health of both pets and humans.

关键词： Ceftazidime-avibactam (CZA) Escherichia coli (E. coli) Pets animal hospital environment Veterinarians

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The evolution,pathogenicity and transmissibility of quadruple reassortant H1N2 swine influenza virus in China:A potential threat to public health

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Virologica Sinica 2024年第2期39卷 205-217页

作者： Xinxin Cui Jinhuan Ma Zifeng Pang Lingzhi Chi Cuishan Mai Hanlin Liu Ming Liao Hailiang Sun College of Veterinary Medicine South China Agricultural UniversityGuangzhou510642China Key laboratory of Zoonosis Control and Prevention of Guangdong Province South China Agricultural UniversityGuangzhou510642China National and Regional Joint Engineering laboratory for Medicament of Zoonosis Prevention and Control South China Agricultural UniversityGuangzhou510642China Shandong Vocational animal Science and Veterinary College Weifang261061China Institute of animal Health Guangdong Academy of Agricultural SciencesGuangzhou510640China

Swine are regarded as“intermediate hosts”or“mixing vessels”of influenza viruses,capable of generating strains with pandemic potential.From 2020 to 2021,we conducted surveillance on swine H1N2 influenza(swH1N2)viruses in swine farms located in Guangdong,Yunnan,and Guizhou provinces in southern China,as well as Henan and Shandong provinces in northern China.We systematically analyzed the evolution and pathogenicity of swH1N2 isolates,and characterized their replication and transmission abilities.The isolated viruses are quadruple reassortant H1N2 viruses containing genes from pdm/09 H1N1(PB2,PB1,PA and NP genes),triple-reassortant swine(NS gene),Eurasian Avian-like(HA and M genes),and recent human H3N2(NA gene)lineages.The NA,PB2,and NP of SW/188/20 and SW/198/20 show high gene similarities to A/Guangdong/Yue Fang277/2017(H3N2).The HA gene of swH1N2 exhibits a high evolutionary rate.The five swH1N2 isolates replicate efficiently in human,canine,and swine cells,as well as in the turbinate,trachea,and lungs of mice.A/swine/Shandong/198/2020 strain efficiently replicates in the respiratory tract of pigs and effectively transmitted among them.Collectively,these current swH1N2 viruses possess zoonotic potential,highlighting the need for strengthened surveillance of swH1N2 viruses.

关键词： Swine influenza virus(SIV) H1N2 Evolution Replication Pathogenicity Zoonotic potential

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Characterization of a pangolin SARS-CoV-2-related virus isolate that uses the human ACE2 receptor

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Science China(Life Sciences) 2024年第7期67卷 1502-1513页

作者： Luo-Yuan Xia Xue-Feng Wang Xiao-Ming Cui Yi-Ming Zhang Zhen-Fei Wang En-Tao Li Chang-Fa Fan Ke Song Yuan-Guo Li Run-Ze Ye Fang-Xu Li Dai-Yun Zhu Jie Zhang Zhuang-Zhuang Shi Ming-Zhu Zhang Liang-Jing Li Shi-Jing Shen Song Jin Ya-Wei Zhang Wei-Guang Fu Lin Zhao Wen-Hao Wang Tie-Cheng Wang You-Chun Wang Jia-Fu Jiang Yan-Ling Hu Na Jia Yu-Wei Gao Wu-Chun Cao Institute of EcoHealth School of Public HealthCheeloo College of MedicineShandong UniversityJinan 250012China Changchun Veterinary Research Institute Changchun 130122China State Key laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and EpidemiologyBeijing 100071China Division of animal Model Research Institute for Laboratory Animal ResourcesNational Institutes for Food and Drug ControlBeijing 102629China National Institutes for Food and Drug Control Beijing 102629China Life Sciences Institute Guangxi Medical UniversityNanning 530020China College of Life Sciences Shandong Normal UniversityJinan 250014China Research Unit of Discovery and Tracing of Natural Focus Diseases Chinese Academy of Medical SciencesBeijing 100071China

Various SARS-CoV-2-related coronaviruses have been increasingly identified in pangolins,showing a potential threat to humans.Here we report the infectivity and pathogenicity of the SARS-CoV-2-related virus,PCoV-GX/P2V,which was isolated from a Malayan pangolin(Manis javanica).PCoV-GX/P2V could grow in human hepatoma,colorectal adenocarcinoma cells,and human primary nasal epithelial cells.It replicated more efficiently in cells expressing human angiotensin-converting enzyme 2(hACE2)as SARS-CoV-2 did.After intranasal inoculation to the hACE2-transgenic mice,PCoV-GX/P2V not only replicated in nasal turbinate and lungs,but also caused interstitial pneumonia,characterized by infiltration of mixed inflammatory cells and multifocal alveolar hemorrhage.Existing population immunity established by SARS-CoV-2 infection and vaccination may not protect people from PCoV-GX/P2V infection.These findings further verify the hACE2 utility of PCoV-GX/P2V by in vivo experiments using authentic viruses and highlight the importance for intensive surveillance to prevent possible cross-species transmission.

关键词： pangolin SARS-CoV-2-related virus infectivity pathogenicity human cell lines human ACE2 receptor hACE2-transgenic mice

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A novel low-cost visual ear tag based identification system for precision beef cattle livestock farming

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Information Processing in Agriculture 2024年第1期11卷 117-126页

作者： Andrea Pretto Gianpaolo Savio Flaviana Gottardo Francesca Uccheddu Gianmaria Concheri Department of Civil Environmental and Architectural EngineeringUniversity of PaduaItaly Department of animal Medicine Production and HealthUniversity of PadovaItaly Department of Industrial Engineering University of PadovaItaly

The precision livestock farming(PLF)has the objective to maximize each animal's performance while reducing the environmental impact and maintaining the quality and safety of meat production.Among the PLF techniques,the personalised management of each individual animal based on sensors systems,represents a viable option.It is worth noting that the implementation of an effective PLF approach can be still expensive,especially for small and medium-sized farms;for this reason,to guarantee the sustainability of a customized livestock management system and encourage its use,plug and play and cost-effective systems are needed.Within this context,we present a novel low-cost method for identifying beef cattle and recognizing their basic activities by a single surveillance camera.By leveraging the current state-of-the-art methods for real-time object detection,(i.e.,YOLOv3)cattle's face areas,we propose a novel mechanism able to detect the ear tag as well as the water ingestion state when the cattle is close to the drinker.The cow IDs are read by an Optical Character Recognition(OCR)algorithm for which,an ad hoc error correction algorithm is here presented to avoid numbers misreading and correctly match the IDs to only actually present IDs.Thanks to the detection of the tag position,the OCR algorithm can be applied only to a specific region of interest reducing the computational cost and the time needed.Activity times for the areas are outputted as cattle activity recognition results.Evaluation results demonstrate the effectiveness of our proposed method,showing a mAP@0.50 of 89%.

关键词： Precision livestock farming Deep learning Cattlei dentification Low-cost sensors Computer vision

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Genetic and biological properties of H9N2 avian influenza viruses isolated in central China from 2020 to 2022

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Journal of Integrative Agriculture 2024年第8期 2778-2791页

作者： Libin Liang Yaning Bai Wenyan Huang Pengfei Ren Xing Li Dou Wang Yuhan Yang Zhen Gao Jiao Tang Xingchen Wu Shimin Gao Yanna Guo Mingming Hu Zhiwei Wang Zhongbing Wang Haili Ma Junping Li College of Veterinary Medicine Shanxi Agricultural University Shanxi Provincial animal Disease Prevention and Control Center

TheH9N2subtypeofavianinfluenzavirus(AIV)iswidelyprevalentinpoultryandwildbirdsglobally,andhas become the predominant subtype circulating in poultry in China.The H9N2 AIV can directly or indirectly (by serving asa“donorvirus”)infecthumans,posingasignificantthreattopublichealth.Currently,thereisalackofindepth research on the prevalence of H9N2 viruses in Shanxi Province, central China.In this study, we isolated 14H9N2 AIVs from October 2020 to April 2022 in Shanxi Province, and genetic analysis revealed that these viruses belonged to 7 different genotypes.Our study on animals revealed that the H9N2 strains we identified displayed hightransmissionefficiencyamongchickenpopulations,andexhibiteddiversereplicationabilitieswithinthese birds.These viruses could replicate efficiently in the lungs of mice, with one strain also demonstrating the capacity to reproduce in organs like the brain and kidneys.At the cellular level, the replication ability of different H9N2 strains was evaluated using plaque formation assays and multi-step growth curve assays, revealing significant differences inthereplicationandproliferationefficiencyofthevariousH9N2virusesatthecellularlevel.Theantigenicity analysis suggested that these isolates could be classified into 2 separate antigenic clusters.Our research provides crucial data to help understand the prevalence and biological characteristics of H9N2 AIVs in central China.It also highlights the necessity of enhancing the surveillance of H9N2 AIVs.

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Simple procedure for assessing diffuse subarachnoid hemorrhage successfully created using filament perforation method in mice

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animal Models and Experimental Medicine 2024年第1期7卷 77-81页

作者： Tatsushi Mutoh Ryota Tochinai Hiroaki Aono Masayoshi Kuwahara Yasuyuki Taki Tatsuya Ishikawa Department of Surgical Neurology Research Institute for Brain and Blood VesselsAkita Cerebrospinal and Cardiovascular CenterAkitaJapan Department of Aging Research and Geriatric Medicine Institute of DevelopmentAging and CancerTohoku UniversitySendaiJapan Department of Veterinary Pathophysiology and animal Health Graduate School of Agriculture and Life SciencesThe University of TokyoTokyoJapan

The murine model of subarachnoid hemorrhage(SAH)is a valuable experimental tool for investigating molecular and cellular mechanisms,and the endovascular filament perforation technique can be used to simulate prominent pathophysiological features observed after human SAH;however,current validation methods for assessing an appropriate SAH model are limited.Here,we introduce a simple procedure for se-lecting a mouse model of diffuse SAH.SAH was induced in 24 mice using a standard filament perforation method.After confirming survival at 24 h,SAH was scored 0-1 based on T2*-weighted images on whole-brain magnetic resonance imaging(MRI)and visual surveillance of the cisterna magna(CM)through the dura mater.The CM-based SAH grading correlated well with a reference parameter defined by extracted brain(r^(2)=0.53,p<0.0001).The receiver operating characteristic curve revealed a sensi-tivity of 85%and a specificity of 91%for detecting diffuse SAH,with a similar area under the curve(0.89±0.06[standard error of the mean])as the MRI-based grading(0.72±0.10,p=0.12).Our data suggest that confirming an SAH clot in the CM is a valuable way to select a clinically relevant diffuse SAH model that can be used in future experimental studies.

关键词： cisterna magna clot distribution filament perforation mouse model subarachnoid hemorrhage

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Towards an actionable One Health approach

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贫困所致传染病(英文) 2024年第2期13卷 1-6页

作者： Zhang Xiao-Xi Lederman Zohar Han Le-Fei Schurer Janna M. Xiao Li-Hua Zhang Zhi-Bing Chen Qiu-Lan Pfeiffer Dirk Ward Michael P. Sripa Banchob Gabri?l Sarah Dhama Kuldeep Acharya Krishna Prasad Robertson Lucy J. Deem Sharon L. Aenishaenslin Cécile Dantas-Torres Filipe Otranto Domenico Grace Delia Wang Yang Li Peng Fu Chao Poeta Patrícia Rahman Md. Tanvir Kassegne Kokouvi Zhu Yong-Zhang Yin Kun Liu Jiming Wang Zhao-Jun Guo Xiao-Kui Gong Wen-Feng Schwartl?nder Bernhard Ren Ming-Hui Zhou Xiao-Nong Sydney School of Veterinary Science The University of Sydney Camden Australia Tropical Disease Research Center Department of Tropical Medicine Faculty of Medicine Khon Kaen University Khon Kaen Thailand laboratory of foodborne parasitic zoonoses Department of translational physiology infectiology and public health Chair Faculty Committee on Internationalisation Faculty of Veterinary Medicine Ghent University Merelbeke Belgium Division of Pathology ICAR-Indian Veterinary Research Institute (IVRI) Bareilly Uttar Pradesh India Department of Livestock Services Animal Quarantine Office-Kathmandu Budhanilkantha Kathmandu Nepal Parasitology Department of Paraclinical Sciences Faculty of Veterinary Medicine Norwegian University of Life Sciences ?s Norway One Government Drive Saint Louis Zoo Institute for Conservation Medicine St. Louis USA Groupe de Recherche en épidémiologie des Zoonoses et Santé Publique (GREZOSP) Faculté de médecine vétérinaire Université de Montréal Saint-Hyacinthe Québec Canada Centre de recherche en santé publique de l Université de Montréal et du CIUSSS du Centre-Sud-de-l’?le-de-Montréal Montréal Québec Canada Department of Immunology Aggeu Magalh?es Institute Oswaldo Cruz Foundation (Fiocruz) Recife Brazil School of Global Health Chinese Center for Tropical Diseases Research Shanghai Jiao Tong University School of Medicine Shanghai People’s Republic of China Department of Veterinary Medicine University of Bari Valenzano Italy Department of Pathobiology Faculty of Veterinary Science Bu-Ali Sina University Hamedan Iran Natural Resources Institute University of Greenwich Chatham Maritime UK International Livestock Research Institute Nairobi Kenya Director of Key laboratory of animal Antimicrobial Resistance surveillance Ministry of Agriculture and Rural Affairs Beijing People’s Republic of China Institute of animal Science Chinese Academy of Agricultural Sciences Beijing People’s Republic of China Key laboratory of Ecosystem Network O

Background Despite the increasing focus on strengthening One Health capacity building on global level, challenges remain in devising and implementing real-world interventions particularly in the Asia-Pacific region. Recognizing these gaps, the One Health Action Commission (OHAC) was established as an academic community for One Health action with an emphasis on research agenda setting to identify actions for highest impact.Main text This viewpoint describes the agenda of, and motivation for, the recently formed OHAC. Recognizing the urgent need for evidence to support the formulation of necessary action plans, OHAC advocates the adoption of both bottom-up and top-down approaches to identify the current gaps in combating zoonoses, antimicrobial resistance, addressing food safety, and to enhance capacity building for context-sensitive One Health implementation.Conclusions By promoting broader engagement and connection of multidisciplinary stakeholders, OHAC envisions a collaborative global platform for the generation of innovative One Health knowledge, distilled practical experience and actionable policy advice, guided by strong ethical principles of One Health.

关键词： One Health Global Health One Health Action Commission Research agenda

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Assessment of immunization procedures for foot-and-mouth disease in large-scale pig farms in China based on actual data and dynamics

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animal Diseases 2022年第1期2卷 45-60页

作者： Huarong Ren Zhen Jin Xin Pei Mingtao Li You ming Wang Juan Zhang Complex Systems Research Center Shanxi University030006 ShanxiChina Shanxi Key laboratory of Mathematical Techniques and Big Data Analysis on Disease Control and Prevention 030006 ShanxiChina School of Mathematical Sciences Shanxi University030006 ShanxiChina College of Mathematics Taiyuan University ofTechnology030024 ShanxiChina the laboratory of animal epidemiological surveillance China Animal Health&Epidemiology Center266032 ShandongChina.

Foot-and-mouth disease(FMD)is an acute,highly infectious and pathogenic animal disease.In recent years,with the rapid development of the swine breeding industry in China,pig farms have shown a trend of larger-scale development.Large-scale pig farms employ standardized management,a high level of automation,and a strict_system.However,these farms have a large trading volume,and increased transmission intensity of FMD is noted inside the farm.At present,the main control measure against FMD is pig vaccination.However,a standard for immunization procedures is not available,and currently adopted immunization procedures have not been effectively and systematically evaluated.Taking a typical large-scale pig farm in China as the research subject and considering the breeding pattern,piggery structure,age structure and immunization procedures,an individual-based state probability model is established to evaluate the effectiveness of the immune procedure.Based on numerical simulation,it is concluded that the optimal immunization program involves primary immunization at 40 days of age and secondary immunization at 80 days of age for commercial pigs.Breeding boars and breeding sows are immunized 4 times a year,and reserve pigs are immunized at 169 and 259 days of age.According to the theoretical analysis,the average control reproduction number of individuals under the optimal immunization procedure in the farm is 0.4927.In the absence of immunization,the average is 1.7498,indicating that the epidemic cannot be controlled without immunization procedures.

关键词： Pig farm Foot-and-mouth disease Individual-based state probability model Immunization procedure Infection probability Individual-based control reproduction number

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A serologic investigation of epizootic hemorrhagic disease virus in China between 2014 and 2019

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Virologica Sinica 2022年第4期37卷 513-520页

作者： Yingliang Duan Zhenxing Yang Pei Zhu Lei Xiao Zhanhong Li Zhuoran Li Le Li Jianbo Zhu Yunnan Tropical and Subtropical animal Virus Diseases laboratory Yunnan Animal Science and Veterinary InstituteKunming650000China

Epizootic hemorrhagic disease virus(EHDV)is a member of the genus Orbivirus,family Sedoreoviridae.It was firstly recognized in 1955 to cause a highly fatal disease of wild white-tailed deer in America.So far,EHDV was detected and isolated in many wild or domestic ruminants,and widely distributed all over the world.Although the domestic cattle and sheep infected by EHDV were usually asymptomatic or subclinical,several outbreaks of epizootic hemorrhagic disease(EHD)in deer and cattle had been reported.Many EHDV strains were isolated and sequenced in last two decades in China,which promoted a general serologic investigation of EHDV in China.In this study,18,122 sera were collected from asymptomatic or subclinical domestic ruminants(cattle,cow,yaks,sheep,goats,and deer)in 116 regions belonging to 15 provinces in China.All the sera were tested by EHDV CELISA,and the results were obtained by big data analysis.EHDV infections were detected in the 14 of 15provinces,and only Tibet(average altitude≥4000 m)which was the highest province in China was free of EHDV.The numbers of seropositive collections in both bovine and goat/sheep were in an inverse proportion to the latitude.However,the seropositive rates in bovine were ranged from 0%to 100%,while the seropositive rates in goat/sheep were no more than 50%.The results suggested that bovine was obviously more susceptive for EHDV infection than goat and sheep,therefore might be a major reservoir of EHDV in China.The prevalence of EHDV was consistent with the distribution of Culicoides which were known as the sole insect vectors of EHDV.In particular,the seropositive rates of EHDV were very high in the southern provinces,which required the enhanced surveillance in the future.

关键词： Epizootic hemorrhagic disease virus(EHDV) Prevalence Ruminant China

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