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Fingerprinting Analysis of Saposhnikovia divaricata using ~1H Nuclear Magnetic Resonance Spectroscopy and High Performance Liquid Chromatography

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Journal of Integrative Plant Biology 2010年第9期52卷 782-792页

作者： Yue-Yang Xin An-Jun Deng Guan-Hua Du Jin-Lan Zhang Hai-Lin Qin key laboratory of bioactive substances and resources utilization of chinese herbal medicine Ministry of Education & Institute of Materia Medica Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100050 China

The 1H nuclear magnetic resonance （1H NMR） fingerprints of fractionated non-polar and polar extracts （control substance for plant drug [CSPD] A and B） from the roots of 12 specimens of Saposhnikovia divaricata （Turcz.） Schischk were achieved with Fourier Transform （FT）-NMR spectrometer and assigned by comparison to each other and to the 1H NMR spectra of the isolated individual compounds. These fingerprints were found to be uniform in terms of the specificity for the implication of all 12 specimens being systematically of the same origin. The uniformity was further affirmed by high performance liquid chromatography （HPLC）, which also revealed exactly identical specificity for the identified S. divaricata species with the 1H NMR appearances of corresponding CSPD on the part of the composition of characteristic constituents when comparing to corresponding individual compounds. This investigation unambiguously shows that the specific signals from the chemotaxonomically significant compounds of chromones and coumarins in S. divaricata are exhibited distinctively in the composite features of both 1H NMR fingerprints and HPLC profiles. The 1H NMR and HPLC profiles established can successfully be used as reference for the authentication of the origin of S. divaricata species as well as for chemotaxonomic studies.

关键词： FINGERPRINTS NUCLEAR magnetic resonance spectroscopy HIGH performance liquid chromatography COUMARINS CHEMOTAXONOMY

A New Triterpene from Cynanchum forrestii

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chinese Chemical Letters 2006年第12期17卷 1569-1572页

作者： Yue LIU Jing QU Shi Shan YU Jing LIU You Cai HU key laboratory of bioactive substances and resources utilization of chinese herbal medicine Ministry of Education ＆ Institute of Materia Medica Peking Union Medical College and Chinese Academy of Medical Sciences Beijing 100050

A new triterpene, named cynanotriterpene A, with a ring-A-fission （3, 4-seco） and 13, 27-cyclours-3-oic acid methyl ester skeleton was isolated from the roots of Cynanchum forrestii Schlechter. The structure of the ... 详细信息

关键词： Cynanchumforrestii triterpene X-ray diffraction.

Two New Flavonol Glycosides from Bridelia tomentosa

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chinese Chemical Letters 2006年第10期17卷 1339-1342页

作者： Shi Hui SHU Jin Lan ZHANG Ying Hong WANG Hai Lin QIN Institute of Materia Medica Chinese Academy of Medical Sciences and Peking Union Medical College Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine Ministry of Education Beijing 100050

Two new flavonol glycosides, tamarixetin 3-O-β-D-xylopyranosyl-（1-2）-α-L-ribopyranoside （1） and tamarixetin 3-O-α-L-ribopyranoside （2） were isolated from the leaves of Bridelia tomentosa. Their structures wer... 详细信息

关键词： Bridelia tomentosa flavonol glycosides tamarixetin 3-O-β-D-xylopyranosyl-(1-2)-α-L-ribopyranoside tamarixetin 3-O-α-L-ribopyranoside.

Phylogenetic constrains on mycorrhizal specificity in eight Dendrobium(Orchidaceae) species

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Science China(Life Sciences) 2017年第5期60卷 536-544页

作者： Xiaoke Xing Xueting Ma Jinxin Men Yanhong Chen Shunxing Guo key laboratory of bioactive substances and resources utilization of chinese herbal medicine Ministry of Education Institute of Medicinal Plant Development Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100193 China

Plant phylogeny constrains orchid mycorrhizal(OrM) fungal community composition in some orchids. Here, we investigated the structures of the OrM fungal communities of eight Dendrobium species in one niche to determine whether similarities in the OrM fungal communities correlated with the phylogeny of the host plants and whether the Dendrobium-OrM fungal interactions are phylogenetically conserved. A phylogeny based on DNA data was constructed for the eight coexisting Dendrobium species,and the OrM fungal communities were characterized by their roots. There were 31 different fungal lineages associated with the eight Dendrobium species. In total, 82.98% of the identified associations belonging to Tulasnellaceae, and a smaller proportion involved members of the unknown Basidiomycota(9.67%). Community analyses revealed that phylogenetically related Dendrobium tended to interact with a similar set of Tulasnellaceae fungi. The interactions between Dendrobium and Tulasnellaceae fungi were significantly influenced by the phylogenetic relationships among the Dendrobium species. Our results provide evidence that the mycorrhizal specificity in the eight coexisting Dendrobium species was phylogenetically conserved.

关键词： orchid mycorrhiza mycorrhizal network fungal community composition phylogenetic conservatism

New anthraquinone derivatives from the roots of Berchemia floribunda

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chinese Chemical Letters 2007年第4期18卷 412-414页

作者： Xin Wei Jian Shuang Jiang Zi Ming Feng Pei Cheng Zhang key laboratory of bioactive substances and resources utilization of chinese herbal medicine （Ministry of Education） Institute of Materia Medica Chinese Academy of Medical Sciences and Peking Union Medical College Beifing 100050 China

A new naphthoquinone-anthraquinone dimer named floribundiquinone E （1） and a new anthraquinone 2-acetylphyscion （2） were isolated from the roots of Berchemia floribunda Brongn. Their structures were elucidated on ... 详细信息

关键词： Berchemia floribunda Anthraquinone Floribundiquinone E 2-Acetylphyscion

Triterpenoids from the Roots of Craibiodendron henryi ***

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Journal of Integrative Plant Biology 2007年第11期49卷 1615-1618页

作者： Xiang-Zhong Huang Yue Liu Shi-Shan Yu You-Cai Hu Jing Qu key laboratory of bioactive substances and resources utilization of chinese herbal medicine Ministry of Education & Institute of Matena Medica Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100050 China

A new triterpenoid, ***-p-coumaroyltaraxerol （1）, along with 11 known triterpenoids, taraxerone （2）, taraxerol （3）, 2α,3β,23,24-tetrahydroxyolean-12-en-28-oic acid （4）, oleanolic acid （5）, β-amyrin （6）, 3β,23-dihydroxylursan-12-en- 28-oic acid （7）, 2α,3β-dihydroxyursan-12-en-28-oic acid （8）, 2α,3β,23-trihydroxyursan-12-en-28-oic acid （9）, 2α,3β,24- trihydroxyursan-12-en-28-oic acid （10）, u rsolic acid （11）, and 3-O-acetylursolic acid （12）, was isolated from Craibiodendron henryi W. W. Smith （Ericaceae）. The structures of these compounds were elucidated on the basis of spectral evidence. Antioxidant activity and vasodilator effect of compound 1 were assessed.

关键词： Craibiodendron henryi W. W. Smith Ericaceae triterpenoids.

A new benzofuranolignan and a new flavonol derivative from the stem of Morus australis

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chinese Chemical Letters 2008年第2期19卷 196-198页

作者： Qing Jian Zhang Di Zao Li Ruo Yun Chen De Quan Yu the key laboratory of bioactive substances and resources utilization of chinese herbal medicine Ministry of Education Institute of Materia Medica Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100050 China

A new benzofuranolignan austrafuran A （1） and a new flavonol derivative 5,7,2′,4′-tetrahydroxy-3-methoxyflavone （2） were isolated from the stem of Morus australis. Their structures were determined on the basis o... 详细信息

关键词： Morus australis Austrafuran A Flavonol Stilbenoid

A new benzophenone glycoside from the leaves of Psidium guajava L.

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chinese Chemical Letters 2011年第2期22卷 178-180页

作者： Hui Zheng Fu Jing Zhi Yang Chuang Jun Li Dong Ming Zhang Institute of Materia Medica Chinese Academy of Medical Sciences and Peking Union Medical College(Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal MedicineMinistry of Education)Beijing 100050China

A new benzophenone glycoside,2,6-dihydroxy-4-O-β-D-glucopyranosylbenzophenone（1）,was isolated from the leaves of guajava *** structure was elucidated by spectral and chemical methods.1 showed significant activities... 详细信息

关键词： Psidium guajava L. Benzophenone glycoside 2,6-Dihydroxy-4-O-β-D-glucopyranosylbenzophenone NO

Two new prenylflavonoids from Morus nigra L.

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chinese Chemical Letters 2009年第12期20卷 1469-1471页

作者： Lei Wang Ting Gong Ruo Yun Chen Institute of Materia Medica Chinese Academy of Medical Sciences and Peking Union Medical College The Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine Ministry of Education Beijing 100050 China

Two new prenylflavonoids, momigrol E（1） and mornigrol F（2） were isolated from Morus nigra L. Their structures were elucidated on the basis of spectroscopic methods, especially 2D-NMR techniques.

关键词： Morus nigra Moraceae Mornigrols E and F Prenylflavonoid