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PICRUSt2 functionally predicts organic compounds degradation and sulfate reduction pathways in an acidogenic bioreactor

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Frontiers of Environmental Science & Engineering 2022年第4期16卷 81-93页

作者： Jun Li Aimin Li Yan Li Minhui Cai Gan Luo Yaping Wu Yechao Tian Liqun Xing Quanxing Zhang State key laboratory of Pollution Control and resources Reuse School of the EnvironmentNanjing UniversityNanjing 210023China Jiangsu key laboratory of Chemical Pollution Control and resources Reuse School of Environmental and Biological EngineeringNanjing University of Science and TechnologyNanjing 210094China

For comprehensive insights into the influences of sulfate on performance,microbial community and metabolic pathways in the acidification phase of a two-phase anaerobic system,a laboratory-scale acidogenic bioreactor was continuously operated to treat wastewater with elevated sulfate concentrations from 2000 to 14000 mg/*** results showed that the acidogenic bioreactor could achieve sulfate reduction efficiency of greater than 70%for influent sulfate content less than 12000 mg/*** sulfate induced the accumulation of volatile fatty acids(VPAs),especially propionate and butyrate,which was the primary negative effects to system performance under the high-sulfate ***-throughput sequencing coupled with PICRUSt2 uncovered that the accumulation of VFAs was triggered by the decreasing of genes encoding short-chain acyl-CoA dehydrogenase(EC:1.***.8.1),regulating the transformation of propanoyl-CoA to propenoyl-CoA and butanoyl-CoA to crotonyl-CoA of propionate and butyrate oxidation pathways,which made these two process hardly ***,genes encoding(EC:1.***.8.1)were mainly carried by order *** was the most abundant sulfate-reducing bacteria and identified as the primary host of dissimilatory sulfate reduction ftinctional *** analysis indicated the dissimilatory sulfate reduction process predominated under a low sulfate environment,but was not favored under the circumstance of *** the increase of sulfate,the assimilatory sulfate reduction process finally overwhelmed dissimilatory as the dominant sulfate reduction pathway in acidogenic bioreactor.

关键词： Acidogenic phase reactor High-sulfate wastewater Sulfate reduction Acidogenic fermentation PICRUSt2

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Effects of the heavy metal cadmium on photosynthetic activity and the xanthophyll cycle in Phaeodactylum tricornutum

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Journal of Oceanology and Limnology 2018年第6期36卷 2194-2201页

作者： JI Yan XIE Xiujun WANG Guangce School of biological and Chemical Engineering Qingdao Technical College key laboratory of Experimental Marine biology Institute of OceanologyChinese Academy of Sciences laboratory for Marine biology and Biotechnology Qingdao National Laboratory for Marine Science and Technology

A bstract Cadmium(Cd) is one of the most common and widespread heavy metals in the environment. Cd has adverse effects on photosynthesis that are countered by photosystem I(PSI) and photosystem II(PSII); however, the protective responses of these photosystems to heavy metal stress remain unclear. Using the model diatom P haeodactylum tricornutum, a biological indicator that is widely used to assess the impact of environmental toxins, we simultaneously measured the effects of Cd on PSI and PSII and examined the levels of pigments in response to high light treatments before and after Cd exposure. Cd significantly reduced the quantum yield and electron transport rates of PSI and PSII. The quantum yield of non-photochemical energy dissipation in PSI due to donor side limitation increased faster than the quantum yield due to acceptor side limitation. The Cd treatment activated the P. tricornutum xanthophyll cycle under artificial light conditions, as indicated by an increased diatoxanthin content. Xanthophyll is important for photoprotection; therefore, the accumulation of diatoxanthin may down-regulate PSII activities to reduce oxidative damage. Together, our results suggest that the rapid reduction in PSII activities following Cd exposure is an adaptive response to heavy metal stress that reflects the variable exposure to external stressors in the native P. tricornutum environment.

关键词： heavy metal cadmium photosystemⅠ photosystemⅡ Phaeodactylum tricornutum

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Synthesis, Crystal Structure and biological Properties of One Zinc(Ⅱ) Complex Based on 4-Acyl Pyrazolone Derivative

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Chinese Journal of Structural Chemistry 2015年第7期34卷 1019-1027页

作者：何川川李玥赵晶许贯诚孙素荣 key laboratory of Material and technology for Clean Energy Ministry of EducationKey Laboratory of Advanced Functional Materials Autonomous RegionInstitute of Applied ChemistryXinjiang University Xinjiang key laboratory of biological resources and Genetic Engineering College of Life Science and Technology Xinjiang University

[Zn（L）（CH3OH）3]（H2L = N-（1-phenyl-3-methyl-4-propenylidene-5-pyrazolone）-salicylidene hydrazide） has been synthesized by the reaction of zinc nitrate and ligand H2 L. The complex crystallizes in orthorhombic system, space group Pbca with a = 5.888（15）, b = 14.564（14）, c = 22.20（2） , V = 5137（8） 3, Z = 8 and F（000） = 2184. The ligand serves as a negative bivalent tridentate chelating agent to coordinate with the central zinc（II） atom. DNA-binding was studied by UV-Vis spectral analysis and ethidium bromide（EB） displacement experiments. The results showed that the DNA-binding constant of the complex is 5.1×104 M–1. Antitumor activity of [Zn（L）（CH3OH）3] and the ligand have been investigated by MTT assay, which indicated that the complex has better cytotoxicity to Eca-109 and He La than free ligand.

关键词： zinc(Ⅱ) complex 4-acyl pyrazolone derivative crystal structure DNA-binding antitumor activity

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Biomolecular Preservation of Miocene Fossil Liquidambar from Tiantai,China and Clarkia,USA:Implications for Palaeoenvironmental Study

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Acta Geologica Sinica(English Edition) 2015年第4期89卷 1076-1087页

作者： XIAO Liang YANG Hong HE Yuli HE Wenlong LI Xiangchuan GUO Junfeng HUI Kaixuan College of Earth Science and resources and Key Laboratory of Western Mineral Resources and Geological Engineering Ministry of EducationChang’an University laboratory of Terrestrial Environments Department of Science and TechnologyCollege of Arts and SciencesBryant University School of Earth Sciences and key laboratory of Mineral resources in western China (Gansu Province) Lanzhou University College of Geology and Environment Xi’an University of Science and Technology

Biomolecular characteristics of fossil Liquidambar from the Miocene Shengxian Formation, Tiantai, Zhejiang province in eastern China and Miocene Clarkia sediment, Idaho, U.S.A., are obtained by means of Pyrolysis-Gas chromatography-Mass spectrometry analysis （Py-GC-MS）. Their modern equivalent （Liquidambarformosana） collected from Nanjing, eastern China, is also analyzed for comparison. The total ion chromatograms （TIC） of modern L. formosana leaves shows abundant pyrolysates of iignin, polysaccharide, aliphatic acid, and amino acid. However, only one polysaccharide molecule, i.e., levoglucosan, is retained in fossil Liquidambar leaves from the two Miocene sites with the Tiantai specimen possessing higher intensity. As expected, some lignin pyrolysates including benzene, phenol, guaiacol, vinylphenol and their derivatives, are detected in Miocene Tiantai L. miosinica. However, the lignin components with large molecular weight other than benzene and phenol compounds, are absent in Miocene Clarkia L. pachyphyllum. Abundant pristene and homologous series of n-alk-l-ene/n-alkane pairs with a long retention time are shown in pyrograms of both Tiantai and Clarkia fossils. Based upon our Py-GC-MS data, the molecular preservation in these Liquidambar leaf samples can be ranked as the following （from high to low quality respectively）： modern sample 〉Tiantai sample 〉Clarkia sample. Vinyl phenol, an important compound, is present in modern and Tiantai Liquidambar leaves, while absent in Clarkia sample, implying that vinyl phenol may be sourced from lignin for Liquidambar leaves. Our data support the hypothesis of in situ polymerization for the origin of long-chain homologous series of n-alk-l-ene/n- alkane pairs as leaf alteration products. Our data have implications of the impact of leaf degradation toward biases for palaeoenvironmental reconstruction using stable carbon isotope of fossil plants, and suggest the importance of evaluating molecular preservation of fossil leaf t

关键词： Liquidambar Py-GC-MS Miocene Biomolecules palaeoenvironment

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Thermodynamic Properties and Relative Stability of Polyhydroxylated Dibenzo-p-dioxins Calculated by Density Functional Theory

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Chinese Journal of Structural Chemistry 2011年第3期30卷 354-361页

作者：戴勇石佳奇郑清王遵尧 Department of Chemical and biological Engineering Yancheng Institute of Technology State key laboratory of Pollution Control and resources Reuse Department of EnvironmentNanjing University

In this work, partial thermodynamic properties of polyhydroxylated dibenzo-p-dioxins （PHODDs） are calculated by density functional theory （DFT） with the Gaussian 03 program at the B3LYP/6-311G^＊＊ level. By comparing the total energy Eθ values, it is found that two types of hydrogen bonds exist in PHODDs, one between a hydroxyl and the parent compound （dibenzop-dioxin） with bond energy of approximate 15.7 kJ/mol and the other between two ortho hydroxyl groups with higher bond energy of about 18.3 kJ/mol. Hydrogen bonds have an effect on the conformation stability. On the basis of evaluating the strength of these two types of hydrogen bonds, 75 most stable congeners are ascertained. The relations of calculated thermodynamic parameters （total energy E^θ, zero-point vibrational energy ZPE, correction value of thermal energy Ethθ, heat capacity at constant volume CV^θ） with the number and position of hydroxyl substitution （NPHOS） are also discussed. The results show that the NPHOS models can be used to predict the thermodynamic properties for PHODD congeners. In addition, the values of molar heat capacities at constant pressure （Cp,m） from 200 to 1000 K for PHODD congeners are calculated, and the temperature dependence relation of Cp,m is obtained with the least-squares method.

关键词： PHODDs DFT thermodynamic property NPHOS Cp,m

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A new horizon for neuroscience:terahertz biotechnology in brain research

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Neural Regeneration Research 2025年第2期20卷 309-325页

作者： Zhengping Pu Yu Wu Zhongjie Zhu Hongwei Zhao Donghong Cui Shanghai key laboratory of Psychotic Disorders Shanghai Mental Health CenterShanghai Jiao Tong University School of MedicineShanghaiChina Department of Psychiatry Kangci Hospital of JiaxingTongxiangZhejiang ProvinceChina Shanghai Synchrotron Radiation Facility Shanghai Advanced Research InstituteChinese Academy of SciencesShanghaiChina key laboratory of Interfacial Physics and technology Shanghai Institute of Applied PhysicsChinese Academy of ScienceShanghaiChina National Facility for Protein Science in Shanghai Shanghai Advanced Research InstituteChinese Academy of SciencesShanghaiChina

Terahertz biotechnology has been increasingly applied in various biomedical fields and has especially shown great potential for application in brain *** this article,we review the development of terahertz biotechnology and its applications in the field of *** evidence indicates promising prospects for the use of terahertz spectroscopy and terahertz imaging techniques in the diagnosis of amyloid disease,cerebrovascular disease,glioma,psychiatric disease,traumatic brain injury,and myelin *** vitro and animal experiments have also demonstrated the potential therapeutic value of terahertz technology in some neuropsychiatric *** the precise underlying mechanism of the interactions between terahertz electromagnetic waves and the biosystem is not yet fully understood,the research progress in this field shows great potential for biomedical noninvasive diagnostic and therapeutic ***,the biosafety of terahertz radiation requires further exploration regarding its two-sided efficacy in practical *** review demonstrates that terahertz biotechnology has the potential to be a promising method in the field of neuropsychiatry based on its unique advantages.

关键词： biological effect brain neuron neuropsychiatry neuroscience non-thermal effect terahertz imaging terahertz radiation terahertz spectroscopy terahertz technology

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SYNTHESIS AND PROPERTIES OF ORGANO-FLY ASH/POLY ANILINE WITH CORE/SHELL STRUCTURE BASED ON EMULSION POLYMERIZATION

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Chinese Journal of Polymer Science 2012年第6期30卷 808-814页

作者：雷西萍 key laboratory of western Architecture and technology School of Materials Science and EngineeringXi'an University of Architecture and Technology

Organo-fly ash （OFA） was prepared with pretreated fly ash （FA） and hexadecyltrimethyl ammonium bromide （HDTMAB）, and the composites composed of OFA and polyaniline were obtained by emulsion polymerization at different OFA weight ratios （2.0 wt%, 5.0 wt%, 10.0 wt%, 15.0 wt% and 20.0 wt%） in the presence of dodecylbenzenesulfonic acid as dopant and emulsifier. A polymerization procedure was supposed. The electrical conductivities of the composites were tested by the four-probe technique. The chemical structure and crystallinity of the composites were confirmed by FT-IR and X-ray diffraction, respectively. Morphologies of FA, OFA and the composites were observed by SEM. The element analysis was performed by energy dispersive spectrometry. The thermal stability of the composites was analyzed by TGA. The results showed that the electrical conductivity of the composites decreased with increasing the feed weight ratio of OFA, and the lowest value was 0.62 S/cm. HDTMAB and PAn were just adsorbed on the surface of FA and OFA, respectively according to the physical adsorption without destroying the crystalline structure of FA or OFA. The surface became smoother after organification of FA by using HDTMAB, and its content on FA surfaces was about 26.9 wt%. The core/shell structure of the composite was observed by SEM analysis. The composites showed a higher thermal stability than pure PAn by introduction of OFA into this polymerization system, the heat stability of PAn was increased by decreasing 31.8 wt% of weight loss after introducing 20 wt% of OFA.

关键词： Fly ash Polyaniline Hexadecyltrimethyl ammonium bromide Core/shell Emulsion polymerization.

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The Potassium Transporter AtKUP12 Enhances Tolerance to Salt Stress through the Maintenance of the K^(+)/Na^(+)Ratio in Arabidopsis

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Phyton-International Journal of Experimental Botany 2021年第2期90卷 389-402页

作者： Hua Zhang Zhongmin Yang Xilong You Youqiang Heng Yan Wang Xinjiang key laboratory of biological resources and Genetic Engineering College of Life Science and TechnologyXinjiang UniversityUrumqi830046China

Potassium(K^(+))is a necessary nutrient for plant growth and crop *** K^(+)transporter plays crucial roles in the absorption and transport of K^(+)in *** K^(+)transporters in Arabidopsis have been reported,but AtKUP12,which is a member of the KT/KUP/HAK family,has not yet been the subject of relevant in-depth *** the present study,we demonstrated that AtKUP12 plays a crucial role in K^(+)uptake in Arabidopsis under 100μM low-K^(+)and 125 mM salt stress ***12 transcripts were induced by K^(+)deficiency and salt *** analyzed the K^(+)uptake of AtKUP12 using the K^(+)uptake-deficient yeast R5421 and Arabidopsis mutant *** with AtKUP12 rescued the growth defect of mutant yeast and atkup12 mutant plants at the low-K^(+)concentration,which suggested that AtKUP12 might be involved in high-affinity K^(+)uptake in low-K^(+)*** comparison to the wild-type(WT)and atkup12-AtKUP12 complementation lines,atkup12 showed a dramatic reduction in potassium concentration,K^(+)/Na^(+)ratio,and root and shoot growth on 12-day-old seedlings under the salt conditions;however,there was no significant difference between the complementation and WT *** together,these results demonstrate that AtKUP12 might participate in salt tolerance in Arabidopsis through K^(+)uptake and K^(+)/Na^(+)homeostasis.

关键词： AtKUP12 atkup12 mutant K+uptake K^(+)/Na^(+)homeostasis salt tolerance

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Paliurus(Paliureae,Rhamnaceae) from the Miocene of East China and its Macrofossil-based Phylogenetic and Phytogeographical History

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Acta Geologica Sinica(English Edition) 2014年第5期88卷 1364-1377页

作者： LI Xiangchuan WANG Haofei LENG Qin XIAO Liang GUO Junfeng HE Wenlong College of Earth Sciences and resources & key laboratory of western Mineral resources and Geological Engineering of the Ministry of Education Chang'an University School of Earth Sciences & key laboratory of Mineral resources of western China Lanzhou University laboratory of Terrestrial Environments Department of Science and TechnologyCollege of Arts and SciencesBryant UniversitySmithfieldRI 02917USA College of Geology and Environment Xian University of Science and Technology

The extant genus Paliurus Miller is divided into P. ramosissimus and P. spina-christi groups and is diagnostically characterized by distinctive orbicular-winged fruits with a trilocular （or often bilocular） ovary and basally triveined leaves with a subentire to serrate margin. Here, structurally preserved winged fruits and associated compressed leaves of Paliurus （Paliureae, Rhamnaceae） are reported from the Middle-Upper Miocene Shengxian Formation of Tiantai, Zhejiang, eastern China. The fruits can be identified as Paliurus microcarpa Xiang-Chuan Lisp. nov., the diagnostic characters of external morphology and anatomy of which differ from those of both extant and fossil generic members; the leaves can be designated as Paliurus sp. The species based on winged fruits, P. microcarpa, might represent an ancient intermediate link exclusively known across the P. ramosissimus and P. spina-christi groups, showing a closer affinity to the P. ramosissimus group based on the resemblant smaller fruits with a narrower wing, and to the P. spina-christi group based on the similar wing structure and texture, respectively. Morphological analyses of the present and previous fossil fruit records around the world indicate that diversification of Paliurus had begun in northern transpacific regions by the Late Eocene. The macrofossil records, i.e., fruits and leaves of Paliurus globally when projected on paleoenvironmental maps in view of changing continental positions through time （from Eocene to Miocene） display that the past distribution of the genus was much wider than the present and belongs to a tropical and warm temperate realm of the Northern Hemisphere. Therefore, the modern restricted disjunction in tropical and warm temperate regions of East Asia and South Europe （even eastwards West Asia） might represent glacial refugia for some of the generic ancient lineages to survive severe cold at least since the terminal Pliocene.

关键词： paleobotany systematics Paliurus microcarpa winged fruit phytogeography Middle-LateMiocene Zhejiang Province

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Expression of Hepatitis C Virus E2 Ectodomain in *** and Its Application in the Detection of Anti-E2 Antibodies in Human Sera

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Acta Biochimica et Biophysica Sinica 2004年第1期36卷 57-63页

作者： Jing LIU, Xin-Xin ZHANG, Shen-Ying ZHANG, Min LU, Yu-Ying KONG, Yuan WANG, and Guang-Di LI ( State key laboratory of Molecular biology, Institute of Biochemistry and Cell biology, Shanghai Institutes for biological Sciences, the Chinese Academy of Sciences, Shanghai 200031, China 2 Department of Infectious Diseases, Ruijin Hospital, Second Medical University of Shanghai, Shanghai 200025, China ) State key laboratory of Molecular biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences the Chinese Academy of Sciences Shanghai 200031 Chin

The second envelope glycoprotein （E2） of hepatitis C virus has been shown to bind human target cells and has become a major target for the development of anti-HCV vaccines. Anti-E2 antibodies have been suggested to be of clinical significance in diagnosis, treatment and prognosis of hepatitis C. However, large-scale expression and purification of E2 proteins in mammalian cells is difficult. As an alternative, E2 fragment （aa 385-730） with a four-amino-acid mutation （aa 568-571 PCNI to RVTS） was expressed as hexa-histidine-tagged full length protein [E2N730（m）] in E. coli and purified to over 85% purity. Purified E2N730（m） was specifically recognized by homologous hepatitis C patient serum in western blot, suggesting that it displayed E2specific antigenicity. Rabbit antiserum raised against E2N730（m） recognized E2 glyco-proteins expressed in mammalian cells in western blot. Purified E2N730（m） was used to detect anti-E2 antibodies in human sera and showed better specificity and sensitivity than previousl

关键词： hepatitis C virus envelope protein E2 expression and purification Escherichia coll

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