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In Vitro Propagation of Ardisia mamillata Hance

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Agricultural Science & Technology 2015年第10期16卷 2159-2161,2166页

作者： Bihua CHEN Juan ZHANG Zhuoxi WU Huihua FAN Qianzhen LI Fujian Academy of forestry Sciences key laboratory of timber forest breeding and cultivation for mountainous areas in southern china China Forestry Bureau key laboratory of timber forest breeding and cultivation for mountainous areas in southern china China Forestry Bureau

Ardisia mamillata Hance is a rare plant with highly ornamental and medicinal value. The traditional propagation methods for A. mamillata by seeds or cutting provided low proliferation rate. This study is to optimize the propagation technique of A. mamillata by tissue culture and set up an industrial production system to provide plenty of A. mamillata seedlings for the human demand. The optimal initiation medium for A. mamillata is MS +2.0 mg/L BA +0.1 mg/L NAA +30 g/L sugar, providing76.4% initiation rate. The optimal shoot proliferation medium for A. mamillata is MS+1.0 mg/L BA+0.1 mg/L NAA+30 g/L sugar, providing 4.56 fold proliferation rate and3.10 cm shoot in height. The optimal shoot elongation medium for A. mamillata is MS+0.5 mg/L BA+0.1 mg/L NAA+30 g/L sugar, providing 2.77 fold proliferation rate and 4.27 cm shoot in height. The optimal rooting medium for A. mamillata is 1/2MS+0.1 mg/L IBA +15 g/L sugar, providing 99.7% rooting rate, 4.0 roots per individual,7.53 cm root in length and 3.94 cm shoot in height. This provides a reliable mass propagation method for A. mamillata.

关键词： Ardisia mamillata In vitro Propagation Medium Red-core soil

Transcriptome Analysis of Auxin Drives Cone Size Regulation in Fokienia hodginsii

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Phyton-International Journal of Experimental Botany 2023年第6期92卷 1713-1723页

作者： Shunde Su Huan Chang Xiangyang Kang Renhua Zheng Beijing Advanced Innovation Center for Tree breeding by Molecular Design National Engineering Laboratory for Tree BreedingKey Laboratory of Genetics and Breeding in Forest Trees and Ornamental PlantsMinistry of EducationCollege of Biological Sciences and TechnologyBeijing Forestry UniversityBeijing100083China key laboratory of timber forest breeding and cultivation for mountainous areas in southern china Key Laboratory of Forest Culture and Forest Product Processing Utilization of Fujian ProvinceFuzhou350012China Basic forestry and Proteomics Research Center College of ForestryFujian Provincial Key Laboratory of Haixia Applied Plant Systems BiologyFujian Agriculture and Forestry UniversityFuzhou350002China

As the reproductive organ of the endangered species Fokienia hodginsii,the size of the cones is a constraint on the reproductive renewal of the *** this study,the molecular basis of the influence of cone size on *** was elucidated by comparing the phenotype,biochemistry,and transcriptome of two cultivars of ***(‘FJ431’and‘FJ415’).The two cultivars differed significantly in cone size,with FJ431 having a significantly larger cone size and weight than FJ415,1.32 and 1.90 times that of FJ415,***-Seq analysis of both cultivars retrieved 75,940 genes whose approximate functions were classified as the pathway of response to endogenous stimulus and response to hormone and showed significant differences in the auxin-activated signaling pathway,particularly the MAPK signaling ***,the endogenous IAA content was significantly higher in FJ431 than in FJ415,and 1.58 and 1.29 times more IAA was present in immature and mature cones,***,exogenous IAA treatment significantly induced the expression of the MAPK pathway-related gene TRINITY_DN10564_c0_g1 and significantly inhibited the expression of the MAPK pathwayrelated gene TRINITY_DN17056_c0_*** work suggests that IAA can affect the cone size of ***,most probably through the MAPK *** has high theoretical and practical significance for the improvement of genetic breeding and the further cultivation of quality germplasm resources of ***.

关键词： Fokienia hodginsii cone size auxin MAPK

In vitro propagation of a medicinal plant: Tripterygium wilfordii Hook f.

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forestry Studies in china 2009年第3期11卷 174-178页

作者： CHEN Bi-hua forestry Biotechnology Research and Development Center Fujian Academy of Forestry Sciences Fuzhou 350012 P. R. China key laboratory of timber forest breeding and cultivation for mountainous areas in southern china State Forestry Administration Fuzhou 350012 P. R. China

In this study a reliable protocol was developed for the establishment of commercial in vitro cultures of Tripterygium wilfordii Hook f.. Juvenile shoots from one-year-old elite plants were used as the source of explants. New axillary shoots were obtained after 30 days of culture on a MS medium supplemented with BAP （2.0 mg.L^-1） and NAA （0.1 mg.L^-1）. The optimal multiplication medium was a modified MS medium supplemented with BAP （1.0 mg.L^-1） and NAA （0.1 mg.L^-1）. This yielded a multiplication rate of 2.4 for each subculture. Slightly more than 92% of shoots rooted when cultured on a modified MS medium containing IBA （0.2 mg.L^-1） and activated charcoal （0.5 mg.L^-1）. Activated charcoal promoted both a strong and a high rooting rate during the rooting phase. Plantlets were transferred to pots for a short acclimatization stage in a greenhouse where 95% of the plantlets survived. This highly reproducible procedure can be adopted for large-scale propagation of T. wilfordii.

关键词： in vitro propagation Tripterygium wilfordii Hook f. multiplication rooting activated charcoal

Inheritance of growth and survival in two 9-year-old, open-pollinated progenies of an advanced breeding population of Chinese firs in southeastern china

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Journal of forestry Research 2016年第5期27卷 1067-1075页

作者： Renhua Zheng Zhou Hong Shunde Su Liming Bian Hui Xiao Jisen Shi Harry X. Wu Fujian Academy of forestry Fuzhou 350012 People's Republic of China the key laboratory of timber forest breeding and cultivation for mountainous areas in southern china Fuzhou 350012 People's Republic of China State forestry Administration Engineering Research Center of Chinese Fir Fuzhou 350012 People's Republic of China The key laboratory of forest Culture and forest Product Processing Utilization of Fujian Province Fuzhou 350012People's Republic of China Research Institute of Tropical forestry Chinese Academy of Forestry No. 682 Guangshan 1 Rd. Guangzhou 510520People's Republic of China The key laboratory of forest Genetics and Biotechnology Nanjing Forestry University Nanjing 210037 People's Republic of China Department of forest Genetics and Plant Physiology Umea Plant Science Centre Swedish University of Agricultural Sciences 90183 Umea Sweden CSIRO NRCA Black Mountain GPO Box 1600 CanberraACT 2601 Australia

Tree growth traits （tree height, DBH and stem volume） and survival from two 9-year-old, open-pollinated progeny tests of Chinese fir were investigated for heri- tability, genotype × environment interaction, age-age genetic correlation and selection efficiency. The 97 and 79 families planted at two sites were collected from the thirdcycle seed orchard. Individual heritability was estimated between 0.05 and 0.21 for tree height, DBH, and volume and between 0.45 and 1.0 for survival. Family heritability was between 0.20 and 1.14. Significant genotype x envi- ronment interaction was observed for the three growth traits. Type B genetic correlation was between 0.41 and 0.67 with an increasing trend as tree grows. High age-age genetic correlation was observed with correlation reaching 0.9 after age 4 for height, DBH, and volume. The genetic gains were estimated at 3.26, 3.39 and 5.98 % for tree height, DBH, and volume with 10 % selection intensity. The implication for advanced tree breeding in Chinese fir is discussed.

关键词： Chinese fir - Progeny test Heritability -Genetic gain

Site index for Chinese fir plantations varies with climatic and soil factors in southern china

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Journal of forestry Research 2022年第6期33卷 1765-1780页

作者： Xiaoyan Li Aiguo Duan Jianguo Zhang State key laboratory of Tree Genetics and breeding Key Laboratory of Tree Breeding and Cultivation of National Forestry and Grassland AdministrationResearch Institute of ForestryChinese Academy of ForestryBeijing 100091People’s Republic of China Collaborative Innovation Center of Sustainable forestry in southern china Nanjing Forestry UniversityNanjing 210037People’s Republic of China

Chinese fir[Cunninghamia lanceolata(Lamb.)Hook.]has a large native distribution range in southern ***,we tested differences in productivity of Chinese fir plantations in different climatic regions and screened the main environmental factors affecting site productivity in each *** of a Chinese fir site index with climatic factors and the soil physiochemical properties of five soil layers were examined in a long-term positioning observation trial comprising a total of 45 permanent plots in Fujian(eastern region in the middle subtropics),Guangxi(south subtropics)and Sichuan(central region in the middle subtropics)in southern *** mixed effects models were developed to predict the site index for Chinese fir,which was found to vary significantly among different climatic *** P,total N,bulk density and total K were dominant predictors of site index in three climatic *** regional linear mixed models built using these predictors in the three climatic regions fit well(R~2=0.86–0.97).For the whole study area,the available P in the 0–20-cm soil layer and total N in the 80–100-cm soil layer were the most indicative soil *** was the most important climatic variable influencing the site *** model evaluation results showed that the fitting performance and prediction accuracy of the global site index model using the climatic region as the dummy variable and random parameters and the most important soil factors of the three climatic regions as predictors was higher than that of global site index model using the climatic variable and the most indicative soil variables of the whole study *** results will help with further evaluation of site quality of Chinese fir plantations and the selection of its appropriate sites in southern china as the climatic changes.

关键词： Site productivity Site index Climate Soil Chinese fir

Genome assembly of wild loquat (Eriobotrya japonica) and resequencing provide new insights into the genomic evolution and fruit domestication in loquat

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Horticulture Research 2023年第2期10卷 147-159页

作者： Danlong Jing Xinya Liu Qiao He Jiangbo Dang Ruoqian Hu Yan Xia Di Wu Shuming Wang Yin Zhang Qingqing Xia Chi Zhang Yuanhui Yu Qigao Guo Guolu Liang key laboratory of Horticulture Science for southern Mountains Regions of Ministry of Education College of Horticulture and Landscape ArchitectureSouthwest UniversityChongqing 400715China Academy of Agricultural Sciences of Southwest University State Cultivation Base of Crop Stress Biology for Southern Mountainous LandChongqing 400715China

Wild loquats(Eriobotrya japonica Lindl.)provide remarkable genetic resources for studying domestication and breeding improved ***,we generate the first high-quality chromosome-level genome assembly of wild loquat,with 45791 predicted proteincoding *** of comparative genomics indicated that loquat shares a common ancestor with apple and pear,and a recent whole-genome duplication event occurred in loquat prior to its *** resequencing showed that the loquat germplasms can be distinctly classified into wild and cultivated groups,and the commercial cultivars have experienced allelic *** with cultivated loquats,the wild loquat genome showed very few selected genomic regions and had higher levels of genetic ***,whole-genome scans of selective sweeps were mainly related to fruit quality,size,and f lesh color during the domestication ***-scale transcriptome and metabolome analyses were further performed to identify differentially expressed genes(DEGs)and differentially accumulated metabolites(DAMs)in wild and cultivated loquats at various fruit development *** those in wild loquat,the key DEGs and DAMs involved in carbohydrate metabolism,plant hormone signal transduction,f lavonoid biosynthesis,and carotenoid biosynthesis were significantly regulated in cultivated loquats during fruit *** high-quality reference genome,resequencing,and large-scale transcriptome/metabolome data provide valuable resources for elucidating fruit domestication and molecular breeding in loquat.

关键词： process insight breeding

Selection of superior families of Pinus massoniana in southern china for large-diameter construction timber

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Journal of forestry Research 2020年第2期31卷 475-484页

作者： Zhangqi Yang Hui Xia Jianhui Tan Yuanheng Feng Yongli Huang Guangxi forestry Research Institute Nanning 530002People’s Republic of China key laboratory of Central South Fast-Growing timber cultivation of forestry Ministry of china Nanning 530002People’s Republic of China Guangxi key laboratory of Superior timber Trees Resource cultivation Nanning 530002People’s Republic of China Nanning forestry Research Institute Nanning 530107People’s Republic of China

This study addresses the increasing demand for large-diameter production timber,and considers the time and space variability of half-sib families of Pinus ***,diameter at breast height(DBH)and timber volume of 440 open-pollinated half-sib progeny families were investigated in 14 progeny trials in different years and production *** evaluation of the genetic variation of all half-sib families was carried out during the sustainable rapid growth period and individual volumes were characterized as a major *** analysis showed that there was considerable variance in the growth traits of most families in different years and on different *** variations caused by temporal and spatial changes of the mating system required three selection methods for *** results show that there were differences among the heritabilities of different growth traits by different halfsib *** heritability values of height,DBH and volume were 0.33,0.34 and 0.36,*** superior families were selected in every progeny test,12 were selected in progeny trials by different years and five in different habitat progeny *** superior families(Gui GC553A,Gui GC414A and Gui GC431A)were selected,although in different years and production *** genetic gains of timber volume of these selected r families ranged from 1.20 to 47.00%,which could provide a foundation for superior wood property selection and serve as material for seed improvement and extension in surrounding areas.

关键词： Pinus massoniana Open-pollinated progeny Large-diameter timber Superior family selection Mating system

Comparison of the Effects of Pesticide Application on Biston suppressaria Larvae in Different Damage Stages

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Plant Diseases and Pests 2021年第3期12卷 1-3,8页

作者： Hezai Lin Shouping Cai Xueyou He Liqiong Zeng Haitian Song Qingquan Wu Zhangpu County forestry Bureau Zhangpu 363200China Fujian Academy of forestry Sciences Key Laboratory of Timber Forest Breeding and Cultivation for Mountainous Areas in Southern ChinaState Forestry AdministrationFuzhou 350012China Pinghe Tianma State-owned forest Farm Pinghe363000China

[Objective] The paper was to determine the optimal control period of Biston suppressaria, the most serious defoliator in eucalyptus forests in southern Fujian Province. [Method] By spraying 2% abamectin + Bacilus thuringiensis powder in the forest, the first generation larvae of B. suppressaria was controlled in the early damage stage(May 2), middle damage stage(May 13) and late damage stage(May 30), respectively.[Result] When 500 g of pesticide was sprayed per 667 m^(2) in the early damage stage of the first generation larvae, the population reduction rate was97.5 % at 5 d post spraying. When 500 g of pesticide was sprayed per 667 m^(2) in the middle damage stage, the population reduction rate was 95.3% at 5 d post spraying. When 1 000 g of pesticide was sprayed per 667 m^(2) in the late damage stage, the population reduction rate was 83.9%, conspicuously lower than those in the early and middle damage stage. If pesticides were sprayed in the early and middle damage stage, the second gen-eration larvae would not cause harms, but the second generation larvae would cause slight harms if pesticides were sprayed in the late damage stage.[Conclusion] Control before the middle damage stage has significant effect on improving the control effect and reducing losses while reducing the usage of pesticides.

关键词： Biston suppresaria Larvae Chemical control Control effect