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电热损伤法建立新西兰大白兔宫腔粘连模型（英文）

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2018年第5期19卷 383-389页

作者： Xin-xin XU Lian-bao CAO Zhe WANG Zhen XU Bing-qian ZHANG She-ling WU Sha-sha QI Lei YAN Zi-jiang CHEN Center for reproductive Medicine Shandong Provincial Hospital Affiliated to Shandong University/National Research Center for Assisted Reproductive Technology and Reproductive Genetics/MOE Key Laboratory of Reproductive Endocrinology (Shandong University)/Shandong Provincial Key Laboratory of Reproductive Medicine Center for reproductive Medicine the Fifth People’s Hospital of Jinan

目的:目前宫腔粘连(Intrauterine adhesion,IUA)发病机制及治疗方法仍未明确和统一,建立一个稳定有效的宫腔粘连动物模型是开展相关研究的前提和基础。本文旨在利用电热损伤法构建兔IUA模型,观察和评估该方法的建模效果。创新点:首次提... 详细信息

目的:目前宫腔粘连(Intrauterine adhesion,IUA)发病机制及治疗方法仍未明确和统一,建立一个稳定有效的宫腔粘连动物模型是开展相关研究的前提和基础。本文旨在利用电热损伤法构建兔IUA模型,观察和评估该方法的建模效果。创新点:首次提出利用电热损伤法建立兔宫腔粘连模型,并得出在损伤后7~14天内建立的兔IUA模型是有效的结论。方法:将21只成年雌性新西兰大白兔一侧子宫内膜用医用多功能高频电刀电灼损伤模拟宫腔粘连形成(A组,n=21),另一侧子宫不做处理作为自身对照(B组,n=21)。分别在损伤后7、14和28天收集兔双侧子宫组织,行苏木精-伊红染色法(HE)和Masson染色观察两侧子宫内膜病理改变,并对两侧子宫内膜的腺体个数和内膜纤维化面积比进行统计学分析和比较。另外,将损伤后7天的雌兔与成年雄兔合笼,14天后观察比较两侧子宫胚胎个数。结论:病理组织学观察显示,损伤后7和14天,A组子宫内膜腺体数量较B组减少,差异均有统计学意义(P<0.05);而损伤后30天,A组子宫内膜腺体数量与B组相比差异无统计学意义。损伤后7天,A组内膜纤维化面积较B组增大,差异有统计学意义(P<0.01);A组子宫胚胎个数较B组减少,差异有统计学意义(P<0.01)。综上所述,采用电热损伤法建立的兔宫腔粘连模型在损伤后7~14天是稳定有效的。

关键词：宫腔粘连电热损伤兔模型

The second polar body contributes to the fate asymmetry in the mouse embryo

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National Science Review 2022年第7期9卷 32-42页

作者： Hongbin Jin Yang Han Huasong Wang J.Xiao He Li Weimin Shen Lin Zhang Luxi Chen Shunji Jia Ping Yuan Hui Chen Anming Meng laboratory of Molecular Developmental Biology State Key Laboratory of Membrane BiologyTsinghua-Peking Center for Life Sciences School of Life SciencesTsinghua University Center for reproductive genetics and reproductive Medicine Sun Yat-sen Memorial HospitalSun Yat-sen University Guangzhou laboratory

The polar bodies(PBs) are extruded microcells during oocyte meiosis and generally regarded as inessentials for embryonic development.Therefore,PBs have been widelyused as important materials for pre-implantation genetic diagnosis in human.Here we report that the second PB(PB2) in the mouse zygote may play roles in cell-fate specification and post-implantation development.A subset of mRNAs encoding pluripotency-related factors are enriched in PB2.Nascent proteins maybe synthesized in PB2 after fertilization and transport from PB2 to the zygote before the two-cell stage.The PB2-attached blastomere(pbB) at the two-cell stage,compared to the other blastomere(npbB),likely contributes more descendants to the inner cell mass(ICM) lineage in the blastocyst.Removal of PB2 from the zygote or transient blockage of material exchange between PB2 and the zygote by nocodazole treatment appears to cause a loss of the ICM fate bias of pbB.PB2 removal or nocodazole treatment also results in abnormal post-implantation development.Injection of PB2 lysate into pbB of PB2-removed two-cell-stage embryos may reset the cell-fate preference and rescue post-implantation development.Our data collectively suggest that PB2 would demarcate the earliest cell-fate asymmetry of the mouse zygote and be required for post-implantation development.

关键词： mouse embryo polar body cell fate asymmetry

The effect of SARS-CoV-2 infection on human embryo early development:a multicenter prospective cohort study

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Science China(Life Sciences) 2023年第7期66卷 1697-1700页

作者： Xiaolei Chen Huangcong Shi Cheng Li Wanxia Zhong Linlin Cui Wenjun Zhang Ling Geng Kuona Hu Mei Fang Daimin Wei Junhao Yan Yun Sun Keliang Wu Han Zhao Zi-Jiang Chen Center for reproductive Medicine Shandong UniversityJinan 250012China Research Unit of Gametogenesis and Health of ART-Offspring Chinese Academy of Medical SciencesJinan 250012China key laboratory of reproductive Endocrinology of Ministry of Education Shandong UniversityShandong Key Laboratory of Reproductive MedicineShandong Provincial Clinical Research Center for Reproductive HealthJinan 250012China National Research Center of Assisted reproductive Technology and reproductive genetics Shandong UniversityJinan 250012China Center for reproductive Medicine The Second HospitalCheeloo College of MedicineShandong UniversityJinan 250012China Center for reproductive medicine Ren Ji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai Key Laboratory for Assisted Reproduction and Reproductive GeneticsShanghai 200127China

Dear Editor,Severe acute respiratory syndrome coronavirus 2(SARSCo V-2)infection has swept the globe for 3 years(Zhou et al.,2020).With the nationwide relaxation of controls on the coronavirus disease 2019(COVID-19)epidemic since December 2022 in China,fertility and in vitro fertilization(IVF)centers are receiving increasing numbers of infected patients.

关键词： infection acute

DNA错配修复系统的改变对人类生育能力及相关治疗的影响（英文）

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2016年第1期17卷 10-20页

作者： Min-hao HU Shu-yuan LIU Ning WANG Yan WU Fan JIN key laboratory of reproductive genetics (Zhejiang) Ministry of Educationand Centre of Reproductive MedicineWomen's HospitalSchool of MedicineZhejiang University

简要概括DNA损伤修复系统在人体中的作用和机制,并探讨其改变与人类生殖能力以及通过辅助生殖技术诞生的子代之间的相互影响。希望更多相关工作的进行能够为人类不孕症的预防、诊断和治疗工作建立一个更好的医疗体系。

关键词： DNA错配修复不孕辅助生殖技术后代

Noninvasive preimplantation genetic testing in assisted reproductive technology:current state and future perspectives

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Journal of genetics and Genomics 2020年第12期47卷 723-726页

作者： Jingyi Li Yifeng Liu Yuli Qian Dan Zhang key laboratory of reproductive genetics(Ministry of Education)and Department of reproductive Endocrinology Women's HospitalZhejiang University School of MedicineHangzhouZhejiang 310006China key laboratory of Women's reproductive Health of Zhejiang Province HangzhouZhejiang 310006China

Invasive genetic screening of pre-implantation embryos via biopsied trophectoderm(TE)cells has been in use for more than 20 years,while its benefits in selecting euploid embryos remain controversial.Recent advances in the ability to process embryonic cell-free DNA(cfDNA)from blastocoel fluid(BF)and spent culture media(SCM)of blastocysts in a manner similar to that of a biopsied TE sample provide a potential alternative holding great promise for obtaining cytogenetic information of the embryos without intrusive biopsy of traditional biopsy-based pre-implantation genetic testing(PGT).Several studies have reported even higher diagnostic accuracy in non-invasive PGT(ni-PGT)than conventional PGT.However,there are still several technical challenges to be overcome before ni-PGT can be accepted as a reliable genomic information source of embryo.In this review,we have summarized the emergence and current state of ni-PGT,and discussed our own perspectives on their limitations and future prospect.There is still a long way to go before truly wide clinical application of ni-PGT.

关键词： Pre-implantation genetic testing Spent culture media Blastocoel fluid Cell-free DNA Non-invasive PGT

Comparison of the Developmental Potential and Clinical Results of In Vivo Matured Oocytes Cryopreserved with Different Vitrification Media

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Chinese Medical Journal 2015年第22期128卷 3029-3034页

作者： Mei Li Miao-Miao Wang Hui Liu Ke-Liang Wu Shui-Ying Ma Cheng Li Hai-Bin Zhao Zi-Jiang Chen Center for reproductive Medicine Provincial Hospital Affiliated to Shandong University National Research Center for Assisted Reproductive Technology andReproductive Genetics Key Laboratory for Reproductive Endocrinology of Ministry of EducatLon Shandong Provincial Key Laboratory of Reproductive Medicine Jinan Shandong 250021 China

Background： Oocyte vitrification is widely used throughout the world, but its clinical efficacy is inconsistent and depends on the vitrification media. This study compared the developmental potential and clinical results of in vivo matured oocytes cryopreserved with different vitrification media. Methods： This retrospective study involved vitrified-warmed oocytes at one in vitro fertilization laboratory. Vitrification media kits comprised the MC kit （ethylene glycol （EG] plus 1,2-propanediol [PROH]）, the KT kit （EG plus dimethyl sulphoxide [DMSO]）, and the Modified kit （EG plus DMSO and PROH kit）. Rates of oocyte survival and subsequent developmental potential were recorded and analyzed. The t-test and the Chi-square test were used to evaluate each method＇s efficacy. Results： Oocyte survival rate was significantly higher for the Modified kit （92.0%） than for the MC kit （88.2%）（P 〈 0.05） and the KT kit （77.3%）（P 〈 0.001）. The rate of high-quality embryo development in the Modified kit group （35.8%） was significantly higher than in the MC kit group （29.0%） and the KT kit group （28.3%）（P 〈 0.001 ）. No significant differences were observed in the clinical pregnancy and implantation rates among the MC, KT, and Modified kit groups （37.2% vs. 30.2% vs. 39.6%; 21.9% vs. 18.8% vs. 27.4%, respectively）（P 〉 0.05）. The high-quality embryo rate per warmed oocyte was significantly higher （23.4%） in the Modified kit group than in the other groups （P 〈 0.001）. The embryo utilization and live birth rates per warmed oocyte were the highest in the Modified kit group, but not significantly （P 〉 0.05）. Conclusions： Modified vitrification media are efficient for oocyte vitrification and. with further verification, may be able to replace commercially available media in future clinical applications.

关键词： Fertilization Live Birth Oocytes Pregnancy Vitrification Media

RBM46 is essential for gametogenesis and functions in post-transcriptional roles affecting meiotic cohesin subunits

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Protein & Cell 2023年第1期14卷 51-63页

作者： Yue Lv Gang Lu Yuling Cai Ruibao Su Liang Liang Xin Wang Wenyu Mu Xiuqing He Tao Huang Jinlong Ma Yueran Zhao Zi-Jiang Chen Yuanchao Xue Hongbin Liu Wai-Yee Chan Shandong key laboratory of reproductive Medicine Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityJinan 250012China Center for reproductive Medicine Shandong UniversityJinan 250012China CUHK-SDU Joint laboratory on reproductive genetics School of Biomedical SciencesFaculty of MedicineThe Chinese University of Hong KongHong KongChina key laboratory of reproductive Endocrinology of Ministry of Education Shandong UniversityJinan 250012China key laboratory of RNA Biology Institute of BiophysicsChinese Academy of SciencesBeijing 100101China Fertility Preservation Lab Reproductive Medicine CenterGuangdong Second Provincial General HospitalGuangzhou 510062China key laboratory of Systems Health Science of Zhejiang Province School of Life ScienceHangzhou Institute for Advanced StudyUniversity of Chinese Academy of SciencesHangzhou 310024China Shanghai key laboratory for Assisted Reproduction and reproductive genetics Shanghai 200135China Center for reproductive Medicine Ren Ji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200135China

RBM46 is a germ cell-specific RNA-binding protein required for gametogenesis,but the targets and molecular functions of RBM46 remain unknown.Here,we demonstrate that RBM46 binds at specific motifs in the 3'UTRs of mRNAs encoding multiple meiotic cohesin subunits and show that RBM46 is required for normal synaptonemal complex formation during meiosis initiation.Using a recently reported,high-resolution technique known as LACE-seq and working with low-input cells,we profiled the targets of RBM46 at single-nucleotide resolution in leptotene and zygotene stage gametes.We found that RBM46 preferentially binds target mRNAs containing GCCUAU/GUUCGA motifs in their 3'UTRs regions.In Rbm46 knockout mice,the RBM46-target cohesin subunits displayed unaltered mRNA levels but had reduced translation,resulting in the failed assembly of axial elements,synapsis disruption,and meiotic arrest.Our study thus provides mechanistic insights into the molecular functions of RBM46 in gametogenesis and illustrates the power of LACE-seq for investigations of RNA-binding protein functions when working with low-abundance input materials.

关键词： RBM46 LACE-seq RNA-binding protein meiosis cohesin

Factors of Fetal Origin in the Regulation of Labor Initiation and Preterm Birth

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Maternal-Fetal Medicine 2023年第4期5卷 238-243页

作者： Longkun Ding Lu Gao Department of Physiology Naval Medical UniversityShanghai 200433China Shanghai key laboratory for Assisted Reproduction and reproductive genetics Shanghai 200135China

Preterm birth is the leading cause of mortality and morbidity in newborns and children under 5 years-of-age.In order to improve the survival rate and quality of preterm infants,there is critical need to identify the specific mechanisms underlying the initiation of labor.Pregnancy represents a period of constant interactive dialog between mother and fetus.A disturbance in the pattern of maternal-fetal communication can induce physiological or pathological labor.Although a number of studies have investigated the contributions of maternal factors to the initiation of labor,the concept that fetal organ development and maternal adaptation are coordinated has emerged over recent years,thus emphasizing that factors of fetal origin may serve as hormonal signals for the initiation of labor.In this review,we summarize and discuss several specific mechanisms by which factors of fetal origin may influence parturition during term or preterm labor,including the specific regulation of fetal organs,including the lungs and accessory organs during pregnancy.Future research may focus on the specific pathways by which signals from the fetal lungs and other fetal organs interact with the maternal system to initiate eventual labor.

关键词： Premature birth Fetal origin factor Placenta Fetal membrane Parturition Term labor

Low anti-mullerian hormone decreased clinical pregnancy and increased risk of poor ovarian response in women over 35 years of age

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Chinese Medical Journal 2023年第4期136卷 499-501页

作者： Jiaqi Chang Lihong Xu Yiming Qin Ran Liu Chenxi Li Shanshan Gao Yujie Dang Center for reproductive Medicine Key Laboratory of Reproductive Endocrinology of Ministry of EducationShandong Key Laboratory of Reproductive MedicineShandong Provincial Clinical Research Center for Reproductive HealthNational Research Center for Assisted Reproductive Technology and Reproductive GeneticsCheeloo College of MedicineShandong UniversityJinanShandong 250012China Department of Gynaecology and Obstetrics Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityJinanShandong 250021China Shandong First Medical University JinanShandong 250021China

To the Editor:Infertility affects 15%to 20%of couples worldwide,and assisted reproductive technology(ART)is the most effective treatment strategy.In addition to female age,many other factors,such as antral follicle count(AFC),basal follicle-stimulating hormone(FSH)level,and anti-Mullerian hormone(AMH)level,collectively known as biomarkers for ovarian reserve tests(ORTs),are widely used to predict ovarian responses and assisted-pregnancy outcomes.[1]Among them,AMH and FSH levels were discordant in 20%to 43%of women undergoing ART treatment,and AMH was proven to be superior in predicting live birth rate(LBR).[2]

关键词： pregnancy collective treatment