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Uncovering key salt-tolerant regulators through a combined eQTL and GWAS analysis using the super pan-genome in rice

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National Science Review 2024年第4期11卷 55-69页

作者： Hua Wei Xianmeng Wang Zhipeng Zhang Longbo Yang Qianqian Zhang Yilin Li Huiying He Dandan Chen Bin Zhang Chongke Zheng Yue Leng Xinglan Cao Yan Cui Chuanlin Shi Yifan Liu Yang Lv Jie Ma Wenchuang He Xiangpei Liu Qiang Xu Qiaoling Yuan Xiaoman Yu Tianyi Wang Hongge Qian Xiaoxia Li Bintao Zhang Hong Zhang Wu Chen Mingliang Guo Xiaofan Dai Yuexing Wang Xiaoming Zheng Longbiao Guo Xianzhi Xie Qian Qian Lianguang Shang Shenzhen Branch Guangdong Laboratory of Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural Sciences Institute of Wetland Agriculture and Ecology Shandong Academy of Agricultural Sciences State key laboratory of Rice Biology China National Rice Research Institute National key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop Science Chinese Academy of Agricultural Sciences Yazhouwan National laboratory

For sessile plants, gene expression plays a pivotal role in responding to salinity stress by activating or suppressing specific genes. However, our knowledge of genetic variations governing gene expression in response to salt stress remains limited in natural germplasm. Through transcriptome analysis of the Global Mini-Core Rice Collection consisting of a panel of 202 accessions, we identified 22 345 and 27 610expression quantitative trait loci associated with the expression of 7787 and 9361 e Genes under normal and salt-stress conditions, respectively, leveraging the super pan-genome map. Notably, combined with genome-wide association studies, we swiftly pinpointed the potential candidate gene STG5—a major salt-tolerant locus known as q STS5. Intriguingly, STG5 is required for maintaining Na+/K+homeostasis by directly regulating the transcription of multiple members of the Os HKT gene family. Our study sheds light on how genetic variants influence the dynamic changes in gene expression responding to salinity stress and provides a valuable resource for the mining of salt-tolerant genes in the future.

关键词： super pan-genome expression quantitative trait loci GWAS salt tolerance rice

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Rapid and controllable in-situ self-assembly of main-group metal nanofilms for highly efficient CO_(2)electroreduction to liquid fuel in flow cells

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Nano Research 2024年第6期17卷 5718-5725页

作者： Miao Wang Huaizhu Wang Yaoda Wang Junchuan Liang Mengfei Zhu Jiarui Li Zuoxiu Tie Zhong Jin State key laboratory of Coordination Chemistry MOE Key Laboratory of Mesoscopic ChemistryMOE Key Laboratory of High Performance Polymer Materials and TechnologyJiangsu Key Laboratory of Advanced Organic MaterialsTianchang New Materials and Energy Technology Researc

The electrocatalytic reduction of CO_(2)is a promising pathway to generate renewable fuels and ***,its advancement is impeded by the absence of electrocatalysts with both high selectivity and ***,we present a scalable in-situ thermal evaporation technique for synthesizing series of Bi,In,and Sn nanofilms on carbon felt(CF)substrates with a high-aspect-ratio *** resulting main-group metal nanofilms exhibit a homogeneously distributed and highly exposed catalyst surface with ample active sites,thereby promoting mass transport and ad-/desorption of reaction *** from the unique fractal morphology,the Bi nanofilms deposited on CF exhibit optimal catalytic activities for CO_(2)electroreduction among the designed metal nanofilms electrodes,with the highest Faradaic efficiency of 96.9%for formate production at−1.3 V *** hydrogen electrode(RHE)in *** an industrially relevant current density of 221.4 mA·cm−2 in flow cells,the Bi nanofilms retain a high Faradaic efficiency of 81.7%at−1.1 V(***)and a good long-term stability for formate ***,a techno-economic analysis(TEA)model shows the potential commercial viability of electrocatalytic CO_(2)conversion into formate using the Bi nanofilms *** results offer a green and convenient approach for in-situ fabrication of stable and inexpensive thin-film catalysts with a fractal structure applicable to various industrial settings.

关键词： CO_(2)electroreduction non-noble metal nanofilms flow cells efficiency and selectivity techno-economic analysis

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Finite-time bearing-only formation of first-order multi-agent systems under pinning control

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Science China(Information Sciences) 2024年第5期67卷 331-332页

作者： Chenjun LIU Wei ZHU Fenglan SUN key laboratory of Intelligent analysis and Decision on Complex Systems Chongqing University of Posts and Telecommunications

Formation control of multi-agent systems(MASs) has been applied in various fields, such as autonomous navigation and target encirclement, due to its ability to facilitate coordination and achieve the desired formation or space configuration. Bearing-based formation control offers greater practicality than position-based or distance-based approaches since bearing information is inexpensive and simple to acquire, such as utilizing optical cameras.

关键词： Multi agent systems

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Topology Optimization of Metamaterial Microstructures for Negative Poisson’s Ratio under Large Deformation Using a Gradient-Free Method

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Computer Modeling in Engineering & Sciences 2024年第5期139卷 2001-2026页

作者： Weida Wu Yiqiang Wang Zhonghao Gao Pai Liu State key laboratory of Structural analysis for Industrial Equipment Dalian University of TechnologyDalian116024China

Negative Poisson’s ratio(NPR)metamaterials are attractive for their unique mechanical behaviors and potential applications in deformation control and energy ***,when subjected to significant stretching,NPR metamaterials designed under small strain assumption may experience a rapid degradation in NPR *** address this issue,this study aims to design metamaterials maintaining a targeted NPR under large deformation by taking advantage of the geometry nonlinearity mechanism.A representative periodic unit cell is modeled considering geometry nonlinearity,and its topology is designed using a gradient-free *** unit cell microstructural topologies are described with the material-field series-expansion(MFSE)*** MFSE method assumes spatial correlation of the material distribution,which greatly reduces the number of required design *** conveniently design metamaterials with desired NPR under large deformation,we propose a two-stage gradient-free metamaterial topology optimization method,which fully takes advantage of the dimension reduction benefits of the MFSE method and the Kriging surrogate model ***,we use homogenization to find a preliminary NPR design under a small deformation *** the second stage,we begin with this preliminary design and minimize deviations in NPR from a targeted value under large *** this strategy and solution technique,we successfully obtain a group of NPR metamaterials that can sustain different desired NPRs in the range of[−0.8,−0.1]under uniaxial stretching up to 20% ***,typical microstructure designs are fabricated and tested through *** experimental results show good consistency with our numerical results,demonstrating the effectiveness of the present gradientfree NPR metamaterial design strategy.

关键词： Topology optimization microstructural design negative Poisson’s ratio large deformation

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Enhanced Natural Convection for Accelerating Melting of Phase Change Material in Cellular Structure through Inserting Fin

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Journal of Thermal Science 2024年第2期33卷 548-563页

作者： KONG Dekui ZHANG Yongcun LIU Shutian State key laboratory of Structural analysis Optimization and CAE Software for Industrial EquipmentDalian University of TechnologyDalian116024China

The design of thermal conductivity enhancers(TCE) is quite critical to overcoming the disadvantage of the poor thermal conductivity of phase change materials(PCM).The main contribution of this study is firstly to discuss how to actively enhance natural convection of the melted PCM in cellular structure by the fin formed in the structure under the condition of the same metal mass,apart from simultaneously improving heat conduction,which can boost the heat transfer ***,a tailored hybrid fin-lattice structure(HFS) as TCE is designed and fabricated by additive manufacturing(AM).A two-equation numerical method is applied to study the heat transfer of the PCM,and its feasibility is validated with the experimental *** numerical results indicate that enhanced natural convection and improved heat conduction can be obtained simultaneously when a well-designed fin is embedded into a lattice *** enhanced natural convection results in the improved melting rate and the decreased wall temperature;e.g.,the complete melting time and the wall temperature are reduced by 11.6% and 19.7%,respectively,because of the fin for metal ***,the parameters of HFS including the porosity,pore density,and fin dimension have a great impact on the heat *** enhancement effect of the fin for HFS on the melting rate of the PCM increases as the thermal conductivity of the base material *** example,when the fin is introduced into the lattice structure,the complete melting time is reduced by 24.1% for metal *** summary,this study enables us to obtain a good understanding of the mechanism of the heat transfer and provides necessary experimental data for the structural design of HFS fabricated by AM.

关键词： natural convection heat transfer enhancement phase change material additive manufacturing hybrid fin-lattice structure

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Topology Optimization Method for Microscale Structures Described with Integral Nonlocal Theory

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Acta Mechanica Solida Sinica 2024年第1期37卷 63-71页

作者： Jiayu Li Quhao Li Shutian Liu State key laboratory of Structural analysis Optimization and CAE Software for Industrial EquipmentDalian University of TechnologyDalian 116024China

The integration of additive manufacturing and topology optimization makes it possible to fabricate complex configurations,especially for microscale structures,which can guarantee the realization of high-performance structural ***,topology results often contain microstructures(several multicellular scales)similar to the characteristic length of local macrostructures,leading to errors in structural performance analysis based on classical ***,it is necessary to consider the size effect in topology *** this paper,we establish a novel topology optimization model utilizing the integral nonlocal theory to account for the size *** approach consists of an integral constitutive model that incorporates a kernel function,enabling the description of stress at a specific point in relation to strain in a distant *** optimization structures based on nonlocal theory are presented for some benchmark examples,and the results are compared with those based on classical medium *** material layout exhibits significant differences between the two approaches,highlighting the necessity of topology optimization based on nonlocal theory and the effectiveness of the proposed method.

关键词： Topology optimization Microstructures Integral nonlocal theory Size effect

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CoFe_(2)O_(4) decorated graphene/C_(18)-functionalized mesoporous silica nanocomposites prepared for magnetic enrichment and electrochemical detection of promethazine in beef

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Chinese Chemical Letters 2024年第3期35卷 208-213页

作者： Xingdong Yang Shi-Ming Ying Sen Zhang Jinxia Dai Wei Gao Tian-Qi Wang Jun-Qin Qiao Hong-Zhen Lian Li Mao State key laboratory of Analytical Chemistry for life Science School of Chemistry&Chemical Engineering and Centre of Materials AnalysisNanjing UniversityNanjing 210023China Ministry of Education(MOE)key laboratory of Modern Toxicology School of Public HealthNanjing Medical UniversityNanjing 211166China

Promethazine(PHZ)is used as a sedative in veterinary medicine,and its residue can threaten the health of *** electrochemical detection of PHZ is suitable method for application in the ***,the traditional electroanalysis is difficult to perform directly in meat samples due to matrix *** work integrates magnetic solid-phase extraction and differential pulse voltammetry for highly sensitive and selective determination of PHZ in beef and beef liver for the first ***_(2)O_(4)/graphene coated with C_(18)-functionalized mesoporous silica(MG@mSiO_(2)-C_(18))is synthesized as dispersed magnetic adsorbent to extract *** glassy carbon electrode modified with nitrogen-doped hollow carbon microspheres(HCM)attracts the MG@mSiO_(2)-C_(18)with PHZ,and directly detects the PHZ without elution ***@mSiO_(2)-C_(18)can separate PHZ to avoid the interference of impurities on following detection,and also concentrate PHZ on magnetic ***,the electrode modification with HCM can amplify the electrochemical signal of ***,the integrated PHZ determination method exhibits a wide linear range from 0.08μmol/L to 300μmol/L with a low limit of detection of 9.8 nmol/*** beef sample analysis presents excellent recovery,demonstrating that this protocol is promising for the rapid and onsite detection of PHZ in real meat samples。

关键词： Promethazine(PHZ) Drug residue Magnetic solid phase extraction Electrochemical detection Beef

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Genome mining of sulfonated lanthipeptides reveals unique cyclic peptide sulfotransferases

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Acta Pharmaceutica Sinica B 2024年第6期14卷 2773-2785页

作者： Meng Wang Wen-Wei Li Zhe Cao Jianong Sun Jiang Xiong Si-Qin Tao Tinghong Lv Kun Gao Shangwen Luo Shi-Hui Dong State key laboratory of Applied organic Chemistry College of Chemistry and Chemical EngineeringLanzhou UniversityLanzhou 730000China

Although sulfonation plays crucial roles in various biological processes and is frequently utilized in medicinal chemistry to improve water solubility and chemical diversity of drug leads,it is rare and underexplored in ribosomally synthesized and post-translationally modified peptides(RiPPs).Biosynthesis of RiPPs typically entails modification of hydrophilic residues,which substantially increases their chemical stability and bioactivity,albeit at the expense of reducing water *** explore sulfonated RiPPs that may have improved solubility,we conducted co-occurrence analysis of RiPP class-defining enzymes and sulfotransferase(ST),and discovered two distinctive biosynthetic gene clusters(BGCs)encoding both lanthipeptide synthetase(LanM)and *** expressing these BGCs,we characterized the structures of novel sulfonated lanthipeptides and determined the catalytic details of LanM and *** demonstrate that SslST-catalyzed sulfonation is leader-independent but relies on the presence of A ring formed by *** LanM and ST are promiscuous towards residues in the A ring,but ST displays strict regioselectivity toward *** recognition of cyclic peptide by ST was further *** evaluation underscores the significance of the ST-catalyzed *** study sets up the starting point to engineering the novel lanthipeptide STs as biocatalysts for hydrophobic lanthipeptides improvement.

关键词： Lanthipeptides Sulfotransferases Sulfonation Biosynthesis Genome mining

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Application of "Three-in-One" Strategy Based on MOF-199 in Determination of Phenylenediamine Isomers and Cr(Ⅵ)

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Journal of analysis and Testing 2024年第1期8卷 28-39页

作者： Yueci Xiang Jiantao Wei Huipeng Sun Ruiyi Zhao Zehua Han Shuangtong Wu Xiaoyan Liu Haixia Zhang State key laboratory of Applied organic Chemistry and College of Chemistry and Chemical Engineering Lanzhou UniversityLanzhou 730000China

Industry progressing caused the pollutants into the surface and groundwater,including organic pollutants such as phenylenediamine(PD)and heavy metals such as Cr(Ⅵ).Herein,a simple"three-in-one"strategy based on MOF-199 was *** MOF-199 catalytic system could be used for the rapid detection of Cr(Ⅵ)and o-phenylenediamine(OPD)in linear ranges of 0.5-50.0μmol/L and 7.0-250.0μmol/L,with the limits of detection of 0.1 and 0.5μmol/L,*** method can also efficiently discriminate PD isomers in just 15 ***,Cr(Ⅵ)was reduced to Cr(Ⅲ)and the elimination of OPD was carried *** simple synthesis,high sensitivity,easy operation,and low cost,this method presented a potential platform for on-site detection of pollutants in water samples.

关键词： On-site detection “Three-in-one”strategy Phenylenediamine isomers

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On Klein tunneling of low-frequency elastic waves in hexagonal topological plates

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Applied Mathematics and Mechanics(English Edition) 2024年第7期45卷 1139-1154页

作者： Yuxin YAO Yuansheng MA Fang HONG Kai ZHANG Tingting WANG Haijun PENG Zichen DENG School of Mechanics Civil Engineering and Architecture Northwestern Polytechnical University MIIT key laboratory of Dynamics and Control of Complex Systems Northwestern Polytechnical University State key laboratory of Structural analysis for Industrial Equipment Dalian University of Technology

Incident particles in the Klein tunnel phenomenon in quantum mechanics can pass a very high potential barrier. Introducing the concept of tunneling into the analysis of phononic crystals can broaden the application prospects. In this study, the structure of the unit cell is designed, and the low frequency(< 1 k Hz) valley locked waveguide is realized through the creation of a phononic crystal plate with a topological phase transition interface. The defect immunity of the topological waveguide is verified,that is, the wave can propagate along the original path in the cases of impurities and disorder. Then, the tunneling phenomenon is introduced into the topological valley-locked waveguide to analyze the wave propagation, and its potential applications(such as signal separators and logic gates) are further explored by designing phononic crystal plates. This research has broad application prospects in information processing and vibration control,and potential applications in other directions are also worth exploring.

关键词： topological metamaterial elastic wave Klein tunneling valley-locked waveguide O347.4 + 1 74J05

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