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Chinese Journal of Chemical Engineering 2013年第1期21卷 31-36页

作者：张军伟王艳彭奇均 The key laboratory of Food Colloids and biotechnology Ministry of Education School of Chemical and Material Engineering Jiangnan University

Extraction of theanine from waste liquid of tea polyphenol production was studied in aqueous surfactanttwo-phase system （ASTP） with cationic surfactant （CTAB） and anionic surfactant （SDS）. Results indicate that the region of ASTP is narrow and there is only a two-phase region of cationic surfactant. The increase in concentrations of NaBr and Na2SO4 are beneficial to the formation of ASTP. Theanine concentration in the bottom phase increases with increasing concentration of theanine, whereas the Partition coefficient and extraction rate only change a littlewhen the concentration of theanine is above 0.2 g.L-＇. With the increase of SDS concentration, the phase ratio and the partition coefficient decrease, while the extraction efficiency of theanine increases and the concentration of theanine changes a little in the range from 2.4/7.5 to 2.8/7.2 for SDS/CTAB ratio. The temperature has a notable ef- fect on the concentration of theanine in the bottom phase, partition coefficient and extraction rate of theanine. The increase of waste liquid decreases the phase ratio, increases the concentration and extraction rate of theanine in the bottom ohase, since the orotein and the saccharide enter the bottom nhase with theanine.

关键词： aqueous two-phase system surfactant theanine waste liquid of tea polyphenol production extraction

Well-dispersive K2O-KCl alkaline catalyst derived from waste banana peel for biodiesel synthesis

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Green Energy & Environment 2019年第3期4卷 322-327页

作者： Mingming Fan Hao Wu Min Shi Pingbo Zhang Pingping Jiang The key laboratory of Food Colloids and biotechnology Ministry of Education School of Chemical and Material Engineering Jiangnan University

In this work, a novel alkaline catalyst was synthesized by an economical and effective method of roasting waste banana peel. From XRD, FTIR, SEM, EDS, TGA and CO2-TPD characterization, it was proved that the calcined banana peel catalyst showed the strong alkalinity and well dispersity in microstructure, and K2O-KCl as the main active contents. The calcined banana peel catalyst showed better catalytic performance than the catalysts by physical mixing of K2O and KCl, which was due to good dispersibility of K2O-KCl formed during decomposing of carbon fiber by calcination of banana peel. Furthermore, the calcined banana peel catalyst also performed well in both water-resistant ability and recyclability, indicating their potential for biodiesel production from an efficient, robust, and low-cost catalyst.

关键词： Biodiesel Banana peel Basic catalyst Transesterification

Screening of strains with potential hypoglycemic effect and its application in fermented garlic production

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Systems Microbiology and Biomanufacturing 2023年第4期3卷 602-614页

作者： Miao Ye Chunfeng Liu Shanshan Chen Chengtuo Niu Jinjing Wang Feiyun Zheng Xin Xu Qi Li key laboratory of industrial biotechnology of ministry of education School of BiotechnologyJiangnan UniversityWuxi 214122People’s Republic of China

Microbial fermentation could produce many active substances in green and pollution-free *** recent years,studies on the hypoglycemic ability of microorganisms had become a trending topic in the prevention and treatment of *** is a food containing a variety of biologically active substances and physiological functions,but it is not accepted by the general public because of its strong pungent *** our study,the microorganisms with potential hypoglycemic effect were screened from strains resistant to garlic *** a result,41 strains of Bacillus,157 strains of lactic acid bacteria,and 23 strains of yeast with tolerance of garlic juice were screened from the selected 568 *** primary screening ofα-amylase inhibitor,it was found that 15 Bacillus and 9 lactic acid bacteria had highα-amylase inhibitory ***,afterα-glucosidase inhibitor re-screening,Pediococcus acidilactici Z1 with highα-amylase inhibitory activity andα-glucosidase inhibitory activity was screened *** results of fermented garlic showed that the inhibition rate ofα-amylase andα-glucosidase of garlic from different origins were significantly increased from 59.09 to 63.99%(p<0.01)and from 52.71 to 71.93%(p<0.01)after Z1 fermentation,*** improvement rates ofα-amylase andα-glucosidase were 107.69 to 287.32%,and 197.74 to 239.86%,*** a word,this study provides a theoretical and experimental basis for the development of new functional garlic products.

关键词： Strain screening Potential hypoglycemic effect Pediococcus acidilactici Fermented garlic

Cloning,expression and improvement of catalytic activity of alginate lyase by site-directed mutation

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Systems Microbiology and Biomanufacturing 2022年第3期2卷 555-567页

作者： Yu Lu Jianli Zhou Qiuya Gu Wenhua Yang Liu Yang Xiaobin Yu The key laboratory of industrial biotechnology Ministry of EducationSchool of BiotechnologyJiangnan University1800 Lihu RoadWuxi 214122China

Alginate lyase mainly produces active alginate oligosaccharides(AOS)by degrading alginate viaβ-elimination *** this study,the Pseudoalteromonas ***6B alginate lyase-encoding gene alg6B-7 from polysaccharide lyase(PL)-7 family was successfully cloned,sequenced,expressed in Escherichia *** on rational design and amino acid sequence alignment of the alginate lyase from various sources,four positive mutants were *** specific enzyme activities of four mutants I62A,A99K,V132S,and L157T were 38.84%,42.85%,75.8%and 51.83%higher than that of the wild enzyme,*** K_(cat)/K_(m) values of the four mutants were both increased,and the catalytic efficiency of V132S was 1.92-fold higher than that of the wild enzyme,*** rational design that was employed in this study achieved the dramatic improvement of catalytic activity,which may provide the application potential in industrial production.

关键词： Alginate lyase Rational design Active center Site-directed mutation Catalytic efficiency

Self-assembly of metal-cholesterol oxidase hybrid nanostructures and application in bioconversion of steroids derivatives

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Frontiers of Chemical Science and Engineering 2021年第3期15卷 615-629页

作者： Yu Xin Qiuyue Gao Yu Gu Mengyao Hao Guangming Fan Liang Zhang key laboratory of Industry biotechnology(ministry of education) School of BiotechnologyJiangnan UniversityWuxi 214122China National Engineering laboratory for Cereal Fermentation Technology Jiangnan UniversityWuxi 214122China

A cholesterol oxidase(COD)was hybridized with Ca^(2+),Zn^(2+),Al^(3+),Fe^(2+) and Mn^(2+).After precipitation with PO_(4)^(3-) at 4℃ for 72 h,the resulting pellets were *** scanning electron microscopy assays,the metal-COD complexes revealed flower-like or granular structures after *** transform infrared spectroscopy assay revealed the characteristic peaks of both the enzyme and metal materials.X-ray diffraction analysis indicated that COD was encapsulated in CaHPO_(4)·2H_(2)O-,Zn_(3)(PO_(4))_(2)·4H_(2)O-,AlPO_(4-),FeP_(4-) and Mn_(3)(PO_(4))_(2)·3H_(2)O-based nanostructures,*** scanning calorimetry assay indicated significant increases in thermo-denaturation temperatures from 60.5℃ to 167.02℃,167.02℃,137.70℃,172.85℃ and 160.99℃,*** steroid derivatives as substrates,this enzyme could convert cholesterol,pregnenolone,dehydroepiandrosterone,ergosterol,b-sitosterol and stigmasterol to related single *** in metal-based nanostructures could significantly enhance the initial conversion ratio and reaction stability of the *** addition,substrate selectivity could be affected by various metal ***,using Ca^(2+),Zn^(2+),Al^(3+),Fe^(2+) and Mn^(2+) as hybrid raw materials could help to encapsulate COD in related metal-enzyme nanostructures,and could help to promote the stability and tolerant properties of the enzyme,while also enhancing its catalytic characteristics.

关键词： cholesterol oxidase metal-enzyme hybridization nanostructures sterol derivatives bioconversion

Food synthetic biology-driven protein supply transition: From animal-derived production to microbial fermentation

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Chinese Journal of Chemical Engineering 2021年第2期34卷 29-36页

作者： Yanfeng Liu Xiaomin Dong Bin Wang Rongzhen Tian Jianghua Li Long Liu Guocheng Du Jian Chen Science Center for Future Foods Jiangnan UniversityWuxi 214122China key laboratory of Carbohydrate Chemistry and biotechnology Ministry of EducationJiangnan UniversityWuxi 214122China key laboratory of industrial biotechnology Ministry of EducationJiangnan UniversityWuxi 214122China National Engineering laboratory for Cereal Fermentation Technology Jiangnan UniversityWuxi 214122China

Animal-derived protein production is one of the major traditional protein supply methods,which continues to face increasing challenges to satisfy global needs due to population growth,augmented individual protein consumption,and aggravated environmental ***,ensuring a sustainable protein source is a considerable *** emergence and development of food synthetic biology has enabled the establishment of cell factories that effectively synthesize proteins,which is an important way to solve the protein supply *** review aims to discuss the existing problems of traditional protein supply and to elucidate the feasibility of synthetic biology in the process of protein ***,using artificial bioengineered milk and artificial bioengineered eggs as examples,the progress of food protein supply transition based on synthetic biology has been systematically ***,the future of food synthetic biology as a potential source of protein has been also *** strengthening and innovating the application of food synthetic biology technologies,including genetic engineering and high-throughput screening methods,the current limitations of artificial foods for protein synthesis and production should be ***,the development and industrial production of new food resources should be explored to ensure safe,high-quality,and sustainable global protein supply.

关键词： Artificial food biotechnology Food processing Food synthetic biology Protein source Protein supply

Innovation leading development:a glimpse into three-dimensional bioprinting in Israel

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Bio-Design and Manufacturing 2024年第3期7卷 358-382页

作者： Lujing Gao Zixuan Liu Daniel Dikovsky Jiqian Wang Deqing Mei Lihi Adler-Abramovich Ehud Gazit Kai Tao State key laboratory of Heavy Oil Processing and Centre for Bioengineering and biotechnology College of Chemistry and Chemical EngineeringChina University of Petroleum(East China)Qingdao 266580China Future Science Research Institute Hangzhou Global Scientific and Technological Innovation CentreHangzhou 311200China Zhejiang-Israel Joint laboratory of Self-Assembling Functional Materials Hangzhou 311200China Redefine Meat Ltd. Rehovot 7670110Israel State key laboratory of Fluid Power and Mechatronic Systems School of Mechanical EngineeringZhejiang UniversityHangzhou 310058China key laboratory of Advanced Manufacturing Technology of Zhejiang Province School of Mechanical EngineeringZhejiang UniversityHangzhou 310058China Department of Oral Biology The Maurice and Gabriela Goldschleger School of Dental MedicineFaculty of Medical&Health SciencesTel Aviv UniversityTel Aviv 6997801Israel The Center for Nanoscience and Nanotechnology Tel Aviv UniversityTel Aviv 6997801Israel Department of Materials Science and Engineering Iby and Aladar FleischmanTel Aviv UniversityTel Aviv 6997801Israel The Shmunis school of Biomedicine and Cancer Research George S.Wise Faculty of Life SciencesTel Aviv UniversityTel Aviv 6997801Israel

Three-dimensional(3D)printing has attracted increasing research interest as an emerging manufacturing technology for devel-oping sophisticated and exquisite architecture through hierarchical *** has also been employed in various advanced industrial *** development of intelligent biomedical engineering has raised the requirements for 3D printing,such as flexible manufacturing processes and technologies,biocompatible constituents,and alternative ***,state-of-the-art 3D printing mainly involves inorganics or polymers and generally focuses on traditional industrial fields,thus severely limiting applications demanding biocompatibility and *** this regard,peptide architectonics,which are self-assembled by programmed amino acid sequences that can be flexibly functionalized,have shown promising potential as bioinspired inks for 3D ***,the combination of 3D printing and peptide self-assembly poten-tially opens up an alternative avenue of 3D bioprinting for diverse advanced ***,a small but innovative nation,has significantly contributed to 3D bioprinting in terms of scientific studies,marketization,and peptide architectonics,including modulations and applications,and ranks as a leading area in the 3D bioprinting *** review summarizes the recent progress in 3D bioprinting in Israel,focusing on scientific studies on printable components,soft devices,and tissue *** paper further delves into the manufacture of industrial products,such as artificial meats and bioinspired supramolecular architectures,and the mechanisms,physicochemical properties,and applications of peptide ***,Israel contributes significantly to the field of 3D bioprinting and should thus be appropriately recognized.

关键词： Israel 3D bioprinting Biomanufacturing Peptide self-assembly Integration of industry,education and research

Influence of lanthanum on chloroplast ultrastructure of soybean leaves under ultraviolet-B stress

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Journal of Rare Earths 2009年第2期27卷 304-307页

作者：彭倩周青 The key laboratory of Environmental Science School of Environmental and Civil EngineeringJiangnan UniversityWuxi 214122China The key laboratory of industrial biotechnology Ministry of EducationJiangnan UniversityWuxi 214122China

In order to investigate the effects of lanthanum（Ⅲ） on cell ultrastructure of soybean leaves under elevated ultraviolet-B irradiation （UV-B, 280-320 nm）, the chloroplast ultrastructure of soybean seedlings was studied by hydroponics under laboratory conditions. The resuits showed that the thylakoid in chloroplast was orderly and clearly as soybean leaves were pretreated by La（Ⅲ）. The thylakoid was indis- tinctly disordered, expanded and even indiscoverable in the chloroplast under UV-B stress. The impact on the thylakoid by the high intensity UV-B irradiation （T2） was bigger than that by the low intensity UV-B irradiation （T1）. However, the destruction of the chloroplast structure caused by UV-B stress was alleviated by La（Ⅲ）, and the arrangement of the thylakoid in the chloroplast became orderly and clearly. The effect of the alleviation by La（Ⅲ） under the low intensity UV-B irradiation （T1） was better than that under the high intensity UV-B irradiation （T2）.

关键词： lanthanum ultraviolet-B irradiation soybean leaves chloroplast ultrastructure rare earths

Depolymerized konjac glucomannan: preparation and application in health care

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Journal of Zhejiang university-Science B(Biomedicine & biotechnology) 2018年第7期19卷 505-514页

作者： Min JIANG Heng LI Jin-song SHI Zheng-hong XU school of Pharmaceutical Jiangnan University key laboratory of Carbohydrate Chemistry and biotechnology Ministry of Education School of Pharmaceutical ScienceJiangnan University National Engineering laboratory for Cereal Fermentation Technology Jiangnan University school of biotechnology Jiangnan University

Konjac glucomannan（KGM） is a water-soluble polysaccharide obtained from the roots and tubers of konjac plants. Recently, a degraded product of KGM, depolymerized KGM（DKGM）, has attracted attention because of its low viscosity, improved hydrophily, and favorable physiological functions. In this review, we describe the preparation of DKGM and its prebiotic effects. Other health benefits of DKGM, covering antioxidant and immune activity, are also discussed, as well as its safety. DKGM could be a candidate for use as a tool for the treatment of various diseases, including intestinal flora imbalance, and oxidative-and immune-related disorders.

关键词： Konjac glucomannan Depolymerized konjac glucomannan Prebiotics Immune response Antioxidant

Enhanced catalytic efficiency and substrate specificity of Streptomyces griseus trypsin by evolution‑guided mutagenesis

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Systems Microbiology and Biomanufacturing 2023年第2期3卷 287-297页

作者： Jingcheng Shi Chaofan Duan Bo Pang Yang Wang Guocheng Du Zhen Kang The Science Center for Future Foods Jiangnan UniversityWuxi 214122China The key laboratory of Carbohydrate Chemistry and biotechnology Ministry of EducationSchool of BiotechnologyJiangnan University1800 Lihu RoadWuxi 214122JiangsuChina The key laboratory of industrial biotechnology Ministry of EducationSchool of BiotechnologyJiangnan UniversityWuxi 214122China

Streptomyces griseus trypsin(SGT)is a bacteria-sourced trypsin that could be potentially applied to industrial insulin ***,SGT produced by microbial hosts displayed low catalytic efficiency and undesired preference to lysine *** this study,by engineering theαsignal peptide in Pichia pastoris,we increased the SGT amidase activity to 67.91 U mL^(−1)in shake flask ***,we engineered SGT by evolution-guided mutagenesis and obtained three variants A45S,V177I and E180M with increased catalytic *** this basis,we performed iterative combinatorial mutagenesis and constructed a mutant A45S/V177I/E180M which the amidase activity reached 98 U mL^(−1)in shake flasks and 2506 U mL^(−1)in 3-L fed-batch ***,single mutation T190 to S190 increased the substrate catalytic preference of R to K and the R/K value was improved to 7.5,which was 2 times better than the animal-sourced trypsin.

关键词： Trypsin Enzyme engineering Pichia pastoris Streptomyces griseus