检索结果-南通市图书馆

Cyprininae phylogeny revealed independent origins of the Tibetan Plateau endemic polyploid cyprinids and their diversifications related to the Neogene uplift of the plateau

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China(Life sciences) 2016年第11期59卷 1149-1165页

作者： Xuzhen Wang Xiaoni Gan Junbing Li Yiyu Chen Shunping He key laboratory of aquatic biodiversity and conservation of the chinese academy of sciences Institute of Hydrobiology Chinese Academy of Sciences

Origin and diversification of the Tibetan polyploid cyprinids(schizothoracins) may help us to explore relationships between diversification of the cyprinids and the Tibetan Plateau uplift. Cyprininae phylogeny was analyzed using mitochondrial and nuclear DNA sequences to trace origins of polyploidy and diversifications of schizothoracins. Ancestral states reconstruction for ploidy levels indicated that the Cyprininae was diploid origin and the schizothoracin clades tetraploid origins. There were two diversification rate shifts along with diversification of the cyprinine fishes in response to the Tibetan uplift. The unusual diversification shifts were located to branches subtending the clades of Tibetan polyploid cyprinids. Our analyses suggested that(i) phylogeny of Cyprininae recovered two independent origins of the Tibetan polyploidy schizothoracins;(ii) diversifications of the schizothoracins were closely related to the Neogene uplift of the Tibetan plateau in the following ways: the relatively ancient Late Oligocene-Middle Miocene adaptive radiation may be associated with the uplift of the southern Tibet and Himalaya; the Middle Miocene-Early Pleistocene lineage-specific diversification broadly coincident with major phase of the Neogene Tibetan uplift; and the most recent Pleistocene diversification shift in Schizothorax closely coincident with the successive Kunlun-Huanghe and Gonghe movements of the Tibetan uplift and the glaciation-induced climate oscillations on the plateau.

关键词： Cyprininae polyploid schizothoracins the Tibetan Plateau diversifications

The hypoxia signaling pathway and hypoxic adaptation in fishes

在线全文

学校读者我要写书评

暂无评论

Science China(Life sciences) 2015年第2期58卷 148-155页

作者： XIAO Wuhan key laboratory of aquatic biodiversity and conservation Institute of Hydrobiology Chinese Academy of Sciences

The hypoxia signaling pathway is an evolutionarily conserved cellular signaling pathway present in animals ranging from Caenorhabditis elegans to mammals.The pathway is crucial for oxygen homeostasis maintenance.Hypoxia-inducible factors(HIF-1αand HIF-2α)are master regulators in the hypoxia signaling pathway.Oxygen concentrations vary a lot in the aquatic environment.To deal with this,fishes have adapted and developed varying strategies for living in hypoxic conditions.Investigations into the strategies and mechanisms of hypoxia adaptation in fishes will allow us to understand fish speciation and breed hypoxia-tolerant fish species/strains.This review summarizes the process of the hypoxia signaling pathway and its regulation,as well as the mechanism of hypoxia adaptation in fishes.

关键词：缺氧诱导因子信号通路低氧适应鱼类信号传导途径哺乳动物适应机制 HIF-1

Neuroendocrine regulation of somatic growth in fishes

在线全文

学校读者我要写书评

暂无评论

Science China(Life sciences) 2015年第2期58卷 137-147页

作者： DAI XiangYan ZHANG Wei ZHUO ZiJian HE JiangYan YIN Zhan key laboratory of aquatic biodiversity and conservation of the chinese academy of sciences Institute of Hydrobiology Chinese Academy of Sciences University of chinese academy of sciences

Growth is a polygenic trait that is under the influence of multiple physiological pathways regulating energy metabolism and muscle growth.Among the possible growth-regulating pathways in vertebrates,components of the somatotropic axis are thought to have the greatest influence.There is growing body of literature focusing on the somatotropic axis and its role regulating growth in fish.This includes research into growth hormone,upstream hypothalamic hormones,insulin-like growth factors,and downstream signaling molecules.Many of these signals have both somatic effects stimulating the growth of tissues and metabolic effects that play a role in nutrient metabolism.Signals of other endocrine axes exhibit profound effects on the function of the somatotropic axis in vivo.In this review we highlight recent advances in our understanding of the teleost fish endocrine somatotropic axis,including emerging research using genetic modified models.These studies have revealed new aspects and challenges associated with regulation of the important steps of somatic growth.

关键词：神经内分泌调节肌肉生长鱼类身体信号分子代谢作用生长轴能量代谢

Variation in the emission rate of sounds in a captive group of false killer whales Pseudorca crassidens during feedings: possible food anticipatory vocal activity?

在线全文

学校读者我要写书评

暂无评论

chinese Journal of Oceanology and Limnology 2016年第6期34卷 1218-1237页

作者： Sara PLATTO 王丁王克雄 key laboratory of aquatic biodiversity and conservation of chinese academy of sciences Institute of Hydrobiology Chinese Academy of Sciences

This study examines whether a group of captive false killer whales(P seudorca crassidens) showed variations in the vocal rate around feeding times. The high level of motivation to express appetitive behaviors in captive animals may lead them to respond with changes of the behavioral activities during the time prior to food deliveries which are referred to as food anticipatory activity. False killer whales at Qingdao Polar Ocean World(Qingdao, China) showed signifi cant variations of the rates of both the total sounds and sound classes(whistles, clicks, and burst pulses) around feedings. Precisely, from the Transition interval that recorded the lowest vocalization rate(3.40 s/m/d), the whales increased their acoustic emissions upon trainers' arrival(13.08 s/m/d). The high rate was maintained or intensifi ed throughout the food delivery(25.12 s/m/d), and then reduced immediately after the animals were fed(9.91 s/m/d). These changes in the false killer whales sound production rates around feeding times supports the hypothesis of the presence of a food anticipatory vocal activity. Although sound rates may not give detailed information regarding referential aspects of the animal communication it might still shed light about the arousal levels of the individuals during different social or environmental conditions. Further experiments should be performed to assess if variations of the time of feeding routines may affect the vocal activity of cetaceans in captivity as well as their welfare.

关键词：假漂亮鲸鱼 Pseudorca crassidens 时间、发信号的可预测性食物先行的活动总计健全的率健全的班评价

Impact of the Three Gorges Dam on reproduction of four major chinese carps species in the middle reaches of the Changjiang River

在线全文

学校读者我要写书评

暂无评论

chinese Journal of Oceanology and Limnology 2016年第5期34卷 885-893页

作者：黎明政段中华高欣曹文宣刘焕章 Institute of Hydrobiology Chinese Academy of Sciences key laboratory of aquatic biodiversity and conservation of chinese academy Of sciences Chinese Academy of Sciences

Silver carp(Hypophthalmichthys molitrix),bighead carp(Aristichthys nobilis),grass carp(Ctenopharyngodon idella),and black carp(Mylopharyngodon piceus) are the four major chinese carps and are commercially important aquaculture species in China.Reproduction of these carp has declined since the construction of the Three Gorges Dam(TGD) due to an altered water flow and thermal regime in the Changjiang(Yangtze) River.However,details of the changes in reproduction of the four species are not well understood.To assess the impact of the TGD on reproduction of the four carp,we investigated their eggs and larvae at Yidu City,which is 80 km below the TGD,during 2005-2012.We examined differences in larval abundance of the four species in the Jianli section(350 km downstream of the TGD) before(1997-2002)and after(2003-2012) construction of the TGD.Based on these observations,the first spawning date of the four species was delayed a mean of about 25 days after the dam was constructed.Mean egg abundance in the Yidu section of the river was 249 million and mean larval abundance was 464 million,which were significant decreases since the 1980 s.Moreover,larval abundance in the Jianli section after the dam was constructed was significantly lower than that before construction(ANCOVA,Pchinese carps species.

关键词： Changjiang(Yangtze) River Three Gorges Dam chinese carp reproduction eggs and larvae

Impact of the Three Gorges Dam on the spawning stock and natural reproduction of chinese sturgeon in the Changjiang River,China

在线全文

学校读者我要写书评

暂无评论

中国海洋湖沼学报：英文版 2016年第5期34卷 894-901页

作者：高欣林鹏程黎明政段中华刘焕章 Institute of Hydrobiology Chinese Academy of Sciences key laboratory of aquatic biodiversity and conservation of chinese academy of sciences Chinese Academy of Sciences

chinese sturgeon(Acipenser sinensis) is the flagship species of the Changjiang River.The migration route of this species is blocked by the first dam,the Gezhou Dam,and its reproduction is affected by the Three Gorges Dam(TGD),one of the largest dams in the world.We studied the impact of the impoundment of the Three Gorges Reservoir(TGR) since 2003 on the spawning stock and the natural reproduction of the chinese sturgeon by using our monitoring data from 1997 to 2013.Results indicate that TGR impoundment has delayed the first spawning dates of the fish from middle-late October to late November,decreased the amount of spawning activities from twice to only once each year,and significantly reduced egg production.In particular,the fish did not demonstrate any spawning activities in 2013.Therefore,TGR impoundment significantly affects the natural reproduction of the fish downstream of the TGD.The spawning stock size of the fish is also predicted to further decrease in the future,which will lead to a risk of population extinction.Ecological regulations must be imposed on decreasing the water temperature to 20℃before mid-October and increasing water discharge downstream of the TGD in October to induce spawning of the chinese sturgeon.

关键词： chinese sturgeon Three Gorges Dam spawning activity spawning stock conservation

Generation and Characterization of a Transgenic Zebrafish Expressing the Reverse Tetracycline Transactivator

在线全文

学校读者我要写书评

暂无评论

Journal of Genetics and Genomics 2013年第10期40卷 523-531页

作者： Qilin Gu Xiaojie Yang Xiaozhen He Qing Li Zongbin Cui The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences Institute of HydrobiologyChinese Academy of Sciences University of chinese academy of sciences

Conditional expression of a target gene during zebrafish development is a powerful approach to elucidate gene functions. The tetracycline-controlled systems have been successfully used in the modulation of gene expression in mammalian cells, but few lines of zebrafish carrying these systems are currently available. In this study, we had generated a stable transgenic zebrafish line that ubiquitously expressed the second-generation of reverse Tet transactivator （rtTA-M2）. Southern blotting analysis and high-throughput genome sequencing verifed that a single copy of rtTA-M2 gene had stably integrated into the zebrafish genome. After induction with doxycycline （Dox）, a strong green fluorescent protein （GFP） was seen in rtTA-transgenic eggs injected with pTRE--EGFP plasmids. The fluorescent signal gradually decreased after the withdrawal of Dox and disappeared. However, leaky expression of GFP was undetectable before Dox- induction. Additionally, transgenic embryos expressing rtTA-M2 exhibited no obvious defects in morphological phenotypes, hatching behavior and expression patterns of developmental marker genes, suggesting that rtTA-M2 had little effect on the development of transgenic zebrafish. Moreover, expressed Dickkopf-1 （DKK1） in pTRE-DKKl-injected embryos led to alterations in the expression of marker genes associated with Wnt signaling. Thus, this rtTA-transgenic zebrafish can be utilized to dissect functions of genes in a temporal manner.

关键词： Zebrafish Transgene Tet-on system Reverse Tet transactivator Doxycycline

Genetic diversity and reproductive success of a wild population of chinese sturgeon(Acipenser sinensis)from the Yangtze River inferred from juveniles born in 2014

在线全文

学校读者我要写书评

暂无评论

Zoological Research 2020年第4期41卷 423-430页

作者： Zhong-Yuan Shen Dan Yu Xin Gao Fu-Tie Zhang Huan-Zhang Liu The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences Institute of HydrobiologyChinese Academy of SciencesWuhanHubei 430072China University of chinese academy of sciences Beijing 100049China

The chinese sturgeon(Acipenser sinensis Gray,1835)is a large anadromous fish species,which is under considerable threat due to dramatic declines in population numbers.In the current study,population genetic diversity and individual reproductive success were assessed using nuclear microsatellite markers(simple sequence repeat,SSR)and complete mitochondrial(mt DNA)genome analysis of juveniles born in 2014.Results showed the existence of size polymorphism in the mt DNA genome of chinese sturgeon,which was caused by a repeat motif.Population genetic diversity was high based on both SSR(Ho:0.728±0.211;He:0.779±0.122)and mt DNA genome analyses(H:0.876±0.0035;Pi:0.0011±0.0010).A positive inbreeding coefficient(FIS:0.066±0.143)was also found,indicating the occurrence of inbreeding.Reconstruction of sibling groups identified 11 mothers and 11 fathers involved in reproduction of chinese sturgeons in 2014.Variance in individual reproductive success was not significant,with reproductive success of parent fish instead shown to be relatively even(P=0.997>0.05),thus suggesting the absence of sweepstakes reproductive success(SRS).These results indicate that,in regard to conservation,loss of genetic diversity due to the effects of SRS is not of particular concern.However,we must focus on having an adequate number of adults and suitable environmental conditions to ensure that fish can reproduce.

关键词： instead absence Yangtze

Genetic diversity and temporal changes of an endemic cyprinid fish species, Ancherythroculter nigrocauda,from the upper reaches of Yangtze River

在线全文

学校读者我要写书评

暂无评论

Zoological Research 2019年第5期40卷 427-438页

作者： Dong-Dong Zhai Wen-Jing Li Huan-Zhang Liu Wen-Xuan Cao Xin Gao key laboratory of aquatic biodiversity and conservation of chinese academy of sciences Institute of Hydrobiology Chinese Academy of Sciences Wuhan Hubei 430072 China University of chinese academy of sciences Beijing 100049 China

Small populations with low genetic diversity are prone to extinction. Knowledge on the genetic diversity and structure of small populations and their genetic response to anthropogenic effects are of critical importance for conservation management. In this study, samples of Ancherythroculter nigrocauda, an endemic cyprinid fish from the upper reaches of Yangtze River, were collected from five sites to analyze their genetic diversity and population structure using mitochondrial cytochrome b gene and 14 microsatellite loci. Haplotype diversity, nucleotide diversity, and expected heterozygosity indicated that the A. nigrocauda populations had low genetic diversity, and decreased heavily from 2001 to 2016. Significant genetic differentiation was found among different populations in the cyt b gene and SSR markers based on the genetic differentiation index (F ST), whereas no differentiation was found in 2001. Haplotype genealogy showed that eight out of 15 haplotypes were private to one population. The SSR STRUCTURE analysis showed that there were four genetic clusters in the A. nigrocauda samples, with each population forming a single cluster, except for the Chishui River (CSR) and Mudong River (MDR) populations, which formed a common cluster. Therefore, loss of genetic diversity and increased genetic differentiation were found in the A. nigrocauda populations, which could be attributed to dam construction, overfishing, and water pollution in the upper Yangtze River. It is therefore recommended that the government should ban fishing, control water pollution, increase river connectivity, and establish artificial breeding and stocking.

关键词： Genetic diversity Population structure Temporal change conservation