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Lysine deficiency impaired growth performance and immune response and aggravated inflammatory response of the skin,spleen and head kidney in grown-up grass carp (Ctenopharyngodon idella)

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animal Nutrition 2021年第2期7卷 556-568页

作者： Yangyang Hu Lin Feng Weidan Jiang Pei Wu Yang Liu Shengyao Kuang Ling Tang Xiaoqiu Zhou animal Nutrition Institute Sichuan Agricultural UniversityChengduChina Fish Nutrition and Safety Production University Key Laboratory of Sichuan ProvinceSichuan Agricultural UniversityChengduChina key laboratory of animal disease-resistant Nutrition Sichuan ProvinceChina key laboratory of animal disease-resistant Nutrition Ministry of EducationChina key laboratory of animal disease-resistant Nutrition and Feed Ministry of Agriculture and Rural AffairsChina animal Nutrition Institute Sichuan Academy of Animal ScienceChengduChina

This dissertation was primarily focused on the immune response,inflammatory response and molecular mechanisms in the skin,head kidney and spleen of grown-up grass carp(Ctenopharyngodon idella).Six iso-nitrogen diets differing in lysine concentrations(5.6,8.5,11.6,14.4,17.5 and 20.7 g/kg)were fed to 540 grass carp(164.85±0.79 g)for 60 *** that,grass carp were challenged by Aeromonas hydrophila for 6 *** study revealed that lysine deficiency(1)suppressed the growth performa,nce of the fish and decreased their ability to resist skin lesion morbidity,(2)impaired the immune organ s immune response by decreasing the gene expressions of mucin,liver-expressed antimicrobial peptide(LEAP)-2B,β-defensin-1 and LEAP-2A and the production of antibacterial compounds of grown-up grass carp,and(3)aggravated the inflammatory response of immune organs in the fish by increasing the gene expressions of pro-inflammatory cytokines(interferon y2[IFN-γ2],tumor necrosis factorα[TNF-α],interleukin[IL]-15,IL-17D,IL-12p40,IL-6 and IL-8)and down-regulating anti-inflammatory cytokines(IL-11,transforming growth factorβ1[TGF-β1],IL-10 and IL-4/13A),which were tightly correlated with signal transducer and activator of transcription(STAT)1 and STAT3 signaling pathway,*** different phenomenon in the skin,spleen and head kidney of fish may be correlated with the difference in gene *** addition,using quadratic regression analysis of percent weight gain(PWG),skin lesion morbidity,and the lysozyme activities in the spleen and head kidney,the dietary lysine requirements for grown-up grass carp were estimated to be 13.58,13.51,14.56 and 14.18 g/kg,respectively.

关键词： Lysine deficiency Grass carp(Ctenopharyngodon idella) Immune function Head kidney Spleen Skin

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Infusion of short chain fatty acids in the ileum improves the carcass traits,meat quality and lipid metabolism of growing pigs

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animal Nutrition 2021年第1期7卷 94-100页

作者： Anran Jiao Hui Diao Bing Yu Jun He Jie Yu Ping Zheng Yuheng Luo Junqiu Luo Quyuan Wang Huifen Wang Xiangbing Mao Daiwen Chen Institute of animal Nutrition Sichuan Agricultural UniversityKey Laboratory for Animal Disease-Resistance Nutrition of China Ministry of EducationKey Laboratory of Animal Disease-resistant Nutrition and Feed of China Ministry of Agriculture and Rural AffairsKey Laboratory of Animal Disease-resistant Nutrition of Sichuan ProvinceYa'an625014China

Short chain fatty acids(SCFA)are the main products of indigestible carbohydrates undergoing bacterial fermentation in the hindgut,which are related to some physiological *** study was designed to investigate the effects of SCFA infusion by ileum on the carcass traits,meat quality and lipid meta-bolism of growing *** a 28-day study,24 growing barrows fitted with a T-cannula in distal ileum were divided into 4 treatments:1)Control,2)antibiotics(AB),3)AB+300 mL of SCFA1 solution(ABS1),4)AB+300 mL of SCFA2 solution(ABS2).The concentrations of acetate,propionate and butyrate in SCFA1 solution were respectively 61.84,18.62 and 12.55 mmol/L,and in SCFA2 were respectively 40.08,15.41 and 9.78 mmol/L The results showed that the SCFA infusion increased the average daily feed intake and average daily gain of pigs(P<0.05).Meanwhile,the SCFA treatments increased longissimus dorsi area(P<0.05)and carcass weight(P=0.058),decreased the drip loss of longissimus dorsi(P=0.059),and reduced serum concentrations of triglyceride,total cholesterol and urea nitrogen(P<0.05).Besides,the SCFA administration inhibited the mRNA expressions of fatty acid synthase(FAS)and acetyl-CoA carboxylase in longissimus dorsi(P<0.05),the mRNA expression of FAS in the liver(P<0.05),and the mRNA expression of hormone-sensitive lipase in abdominal fat(P<0.05).Short chain fatty acid infusion also enhanced the mRNA expression of carnitine palmitoyltransferase-1αin the liver(P<0.05),the mRNA expressions of peroxisome proliferator activated receptor gamma and lipoprotein lipase in abdominal fat(P<0.05),and the mRNA expressions of free fatty acid receptor 2,glucose-6-phosphatase and phosphoenolpyruvate carboxykinase 1 in the colon(P<0.05).These results suggested that SCFA administration in the ileum could improve the carcass traits and meat quality of growing pigs,which was possibly due to the fact that SCFA modulated lipid metabolism.

关键词： Short chain fatty acid Carcass trait Meat quality Lipid metabolism Growing pig

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Bioinformatic approaches of liquid–liquid phase separation in human disease

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中华医学杂志(英文版) 2024年第16期137卷 1912-1925页

作者： Sun Jun Chen Yilong Bi Ruiye Yuan Yong Yu Haopeng Department of Thoracic Surgery and West China Biomedical Big Data Center West China Hospital Sichuan University Chengdu Sichuan China Med-X Center for Informatics Sichuan University Chengdu Sichuan China Department of Orthognathic and TMJ Surgery State Key Laboratory of Oral Diseases National Clinical Research Center for Oral Diseases West China Hospital of Stomatology Sichuan University Chengdu Sichuan China

Biomolecular aggregation within cellular environments via liquid–liquid phase separation (LLPS) spontaneously forms droplet-like structures, which play pivotal roles in diverse biological processes. These structures are closely associated with a range of diseases, including neurodegenerative disorders, cancer and infectious diseases, highlighting the significance of understanding LLPS mechanisms for elucidating disease pathogenesis, and exploring potential therapeutic interventions. In this review, we delineate recent advancements in LLPS research, emphasizing its pathological relevance, therapeutic considerations, and the pivotal role of bioinformatic tools and databases in facilitating LLPS investigations. Additionally, we undertook a comprehensive analysis of bioinformatic resources dedicated to LLPS research in order to elucidate their functionality and applicability. By providing comprehensive insights into current LLPS-related bioinformatics resources, this review highlights its implications for human health and disease.

关键词： Phase separation Liquid–liquid phase separation disease Phase separation databases Bioinformatic tools

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Perspectives of genetic management strategy for inherited cardiovascular diseases in China

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Chinese Medical Journal 2024年第8期137卷 959-961页

作者： Yaoyao Zhang Yanjiang Zheng Mengyuan Dai Kaiyu Zhou Lijun Fu Yuxuan Guo Yihua He Fen Li Rui Gao Donghui Zhang Xujie Liu Jie Tian Yimin Hua Yifei Li Lang Qin key laboratory of Birth Defects and Related diseases of Women and Children of MOE West China Second University HospitalSichuan UniversityChengduSichuan 610041China Department of Department of Obstetrics and Gynecology West China Second University HospitalSichuan UniversityChengduSichuan 610041China Department of Pediatrics West China Second University HospitalSichuan UniversityChengduSichuan 610041China Department of Cardiology Shanghai Children’s Medical CenterSchool of MedicineShanghai Jiao Tong UniversityShanghai 200025China Peking University health Science Center School of Basic Medical SciencesThe Institute of Cardiovascular SciencesKey Laboratory of Molecular Cardiovascular Science of Ministry of EducationBeijing 100191China Department of Ultrasound Beijing Anzhen Hospital affiliated to Capital Medical UniversityBeijing 100029China State key laboratory of Biocatalysis and Enzyme Engineering School of Life ScienceHubei UniversityWuhanHubei 430062China Shenzhen key laboratory of Cardiovascular disease Fuwai Hospital Chinese Academy of Medical SciencesShenzhenGuangdong 518052China Department of Cardiology Ministry of Education Key Laboratory of Child Development and DisordersNational Clinical Research Center for Child Health and Disorders(Chongqing)China International Science and Technology Cooperation Base of Child Development and Critical DisordersChildren’s Hospital of Chongqing Medical UniversityChongqing 400014China

Inherited cardiovascular diseases(CVDs)threaten human health and pose an enormous economic burden *** alteration is a major risk factor for many *** disorders are usually controlled by a pair of alleles,affecting offspring according to the Mendelian principle,regardless of isolated primary damage or secondary injury from other syndromes or *** date,there are hundreds of inherited *** advances in nextgeneration sequencing(NGS)technologies,rapid and accurate molecular diagnosis of patients with inherited CVDs is clinically ***,great improvements have been made in recent years,and targeted therapy and assist devices have been used in clinical *** there is still no totally efficient strategy for dealing with inherited ***.

关键词： diagnosis hundreds dealing

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Characterization of codon usage bias in the dUTPase gene of duck enteritis virus

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Progress in Natural Science:Materials International 2008年第9期18卷 1069-1076页

作者： Lichan Zhao a, Anchun Cheng a,b,, Mingshu Wang a,b,, Guiping Yuan c, Mingsheng Caia a Avian disease Research Center, College of Veterinary Medicine of sichuan Agricultural University, Xinkang Road 46, Ya’an, sichuan 625014, China b key laboratory of animal disease and human health of sichuan province, Ya’an 625014, China c Analytical & Testing Center, sichuan University, Chengdu 610064, China Avian disease Research Center College of Veterinary Medicine of Sichuan Agricultural University Xinkang Road 46 Ya’an Sichuan 625014 China key laboratory of animal disease and human health of sichuan province Ya’an 625014 China Analytical & Testing Center Sichuan University Chengdu 610064 China

A comparative analysis of the codon usage bias in the newly discovered dUTPase gene (Assigned Accession No.:DQ486149) of the duck enteritis virus (DEV) and the dUTPase gene of 32 reference herpesviruses was *** results indicated that the DEV dUT-Pase gene encodes a protein of 477 amino acids, which includes five conserved motifs with a 3-1-2-4-5 *** codon adap-tation index (CAI), efiective number of codons (ENC), and GC3S values indicated synonymous codon usage bias in the dUTPase gene of herpesviruses, and this synonymous bias was correlated with host *** codon usage patterns of the DEV dUTPase gene were phylogenetically conserved and similar to that of the dUTPase genes of the avian *** codon usage in each micro-organism was difierent, there were no strain-specific difierences among ***-one codons in the predicted polypeptide, with a strong bias towards A and T at the third codon position, were *** of the codon usage in the dUTPase gene of difierent organisms revealed that there were 19 codons showing distinct codon usage difierences between the DEV and Escherichia coli dUTPase genes;16 between the DEV and yeast dUTPase genes;and 15 between the DEV and human dUTPase *** of variance (ANOVA) showed significant difierences between the DEV and yeast dUTPase genes (r = 0.536, P < 0.01).The extent of codon usage bias in the DEV dUTPase gene was highly correlated with the gene expression level, therefore the results may provide useful information for gene classification and functional studies.

关键词： DEV dUTPase Codon usage bias EMBOSS

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Exploring the abundance and influencing factors of antimicrobial resistance genes in manure plasmidome from swine farms

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Journal of Environmental Sciences 2023年第2期124卷 462-471页

作者： Zunfang Tu Junrui Shui Jinxin Liu Hongmei Tuo Haoyu Zhang Cong Lin Jingyi Feng Yuxuan Feng Wen Su Anyun Zhang animal disease Prevention and Food Safety key laboratory of sichuan province Key Laboratory of Bio-Resource and Eco-Environment of Ministry of EducationCollege of Life SciencesSichuan UniversityChengdu 610065China laboratory of Gastrointestinal Microbiology Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal HealthCollege of Animal Science and TechnologyNanjing Agricultural UniversityNanjing 210095China

Plasmids play a critical role in the dissemination of antimicrobial resistance genes(ARGs),however,a systematical understanding of ARGs originated from plasmids in swine production is currently ***,quantitative polymerase chain reaction was applied to determine the prevalence of ten ARGs and the class1 integron gene intI1 of plasmid source in swinemanure from 44 farms in sichuan,Hubei and Hebei provinces,*** assayed ARGs were observed in plasmid DNA samples,and the average absolute abundance of aac(6’)-Ib-cr,blaNDM,blaCTX-M,optrA,ermB,floR,mcr-1,qnrS,tetM,sul1 and intI1 were 7.09,2.90,4.67,6.62,7.55,7.14,4.08,4.85,7.16,7.11 and 8.07 of 10 log copies/gram,***1 showed a high correlation(r>0.8,Phealth risks in livestock production.

关键词： Antimicrobial resistance genes Plasmid Swine manure Influencing factors

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Vanadium in high-fat diets sourced from egg yolk decreases growth and antioxidative status of Wistar rats

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animal Nutrition 2019年第3期5卷 307-313页

作者： Jian-Ping Wang Ren-Yong Cui Xue-Mei Ding Shi-Ping Bai Qiu-Feng Zeng Huan-Wei Peng Ke-Ying Zhang animal Nutrition Institute Key Laboratory of Animal Disease-Resistance Nutrition Ministry of Education Ministry of Agriculture and Sichuan Province Sichuan Agricultural University

The objective of this paper was to evaluate the effect of vanadium(V)in high-fat diets sourced from egg yolk on body weight gain,feed intake,blood characteristics and antioxidative status of Wistar rats.A total of 72 female Wistar rats were allocated according to a 2 x 4 factorial design throughout a 5-wk trial,including 2 levels of dietary fat(normal and high;ether extract 40.3 and 301.2 g/kg;fat sourced from egg yolk)and 4 levels of dietary V(0,3,15 and 30 mg/kg).Vanadium decreased(P<0.05)body weight gain(V at 30 mg/kg during wk 1 and 2;V at 15 and 30 mg/kg during the overall phase),feed intake(V at30 mg/kg during wk 3 and the overall phase;V at 15 and 30 mg/kg during wk 4),but increased the relative weight of liver(V at 30 mg/kg,P<0.05).Moreover,increasing dietary V significantly increased(P<0.05)plasma aspartate aminotransferase,alanine aminotransferase and malondialdehyde levels and decreased triglyceride level,and V at 30 mg/kg in high-fat treatment had the highest or lowest values(interaction,P<0.05).Under the same dietary V dose,V residual content in liver(dietary V at 15 and30 mg/kg)and kidney(dietary V at 15 mg/kg)was higher in high-fat diet treatment compared with normal-fat diet treatment(P<0.05).In conclusion,it is suggested that V could decrease the body weight together with the feed intake,and the high fat could enhance oxidative stress induced by V of Wistar rats.

关键词： Vanadium High fat Oxidative stress Body weight Wistar rats

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Yeast hydrolysate attenuates lipopolysaccharide-induced inflammatory responses and intestinal barrier damage in weaned piglets

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Journal of animal Science and Biotechnology 2023年第4期14卷 1569-1583页

作者： Runqi Fu Chan Liang Daiwen Chen Gang Tian Ping Zheng Jun He Jie Yu Xiangbing Mao Yuheng Luo Junqiu Luo Bing Yu key laboratory of animal disease-Resistance Nutrition Ministry of EducationMinistry of Agriculture and Rural AffairsKey Laboratory of Sichuan ProvinceChengdu 611130China Institute of animal Nutrition Sichuan Agricultural UniversityChengdu 611130SichuanChina

Background Intestinal inflammation is the main risk factor causing intestinal barrier dysfunction and lipopolysaccharide(LPS)can trigger inflammatory responses in various eukaryotic *** hydrolysate(YH)possesses multibiological effects and is received remarkable attention as a functional ingredient for improving growth performance and promoting health in ***,there is still inconclusive on the protective effects of dietary YH supplementation on intestinal barrier of *** study was conducted to investigate the attenuate effects of YH supplementation on inflammatory responses and intestinal barrier injury in piglets challenged with *** Twenty-four piglets(with an average body weight of 7.42±0.34 kg)weaned at 21 days of age were randomly assigned to one of two dietary treatments(12 replications with one pig per pen):a basal diet or a basal diet containing YH(5 g/kg).On the 22nd d,6 piglets in each treatment were intraperitoneally injected with LPS at 150μg/kg BW,and the others were injected with the same amount of sterile normal *** hours later,blood samples of each piglet were collected and then piglets were *** Dietary YH supplementation increased average daily feed intake and average daily gain(P<0.01),decreased the ratio of feed intake to gain of piglets(P sponse,evidenced by the increase o=0.048).Lipopolysaccharide(LPS)injection induced systemic inflammatory ref serum concentrations of haptoglobin(HP),adrenocorticotropic hormone(ACTH),cortisol,and interleukin-1β(IL-1β).Furthermore,LPS challenge resulted in inflammatory intestinal damage,by up-regulation of the protein or mRNA abundances of tumor necrosis factor-α(TNF-α),IL-1β,toll-like receptors 4(TLR4)and phosphor-nuclear factor-κB-p65(p-NFκB-p65)(P<0.01),and down-regulation of the jejunal villus height,the protein and mRNA abundances of zonula occludens-1(ZO-1)and occludin(OCC;P<0.05)in jejunal *** YH supplementation decreased the impaired eff

关键词： Inflammatory response Intestinal barrier Lipopolysaccharide Piglets Yeast hydrolysate

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Effect of sialyllactose on growth performance and intestinal epithelium functions in weaned pigs challenged by enterotoxigenic Escherichia Coli

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Journal of animal Science and Biotechnology 2022年第4期13卷 1151-1162页

作者： Qiming Duan Daiwen Chen Bing Yu Zhiqing Huang Yuheng Luo Ping Zheng Xiangbing Mao Jie Yu Junqiu Luo Hui Yan Jun He Institute of animal Nutrition Sichuan Agricultural UniversitySichuan ProvinceChengdu 611130People’s Republic of China key laboratory of animal disease-resistant Nutrition Sichuan ProvinceChengdu 611130People’s Republic of China

Background:Sialyllactose(SL)is one of the most abundant oligosaccharides present in porcine breast ***,little is known about its effect on growth performance and intestinal health in weaned *** study was conducted to explore the protective effect of SL on intestinal epithelium in weaned pigs upon enterotoxigenic Escherichia coli(ETEC)***:Thirty-two pigs were randomly divided into four *** fed with a basal diet or basal diet containing SL(5.0 g/kg)were orally infused with ETEC or culture ***:SL supplementation elevated the average daily gain(ADG)and feed efficiency in the ETEC-challenged pigs(P<0.05).SL also improved the digestibilities of dry matter(DM),gross energy(GE),and ash in non-challenged pigs(P<0.05).Moreover,SL not only elevated serum concentrations of immunoglobulins(IgA,IgG,and IgM),but also significantly decreased the serum concentrations of inflammatory cytokines(TNF-α,IL-1β,and IL-6)upon ETEC challenge(P<0.05).Interestingly,SL increased the villus height,the ratio of villus height to crypt depth(V:C),and the activities of mucosal sucrase and maltase in the jejunum and ileum(P<0.05).SL also elevated the concentrations of microbial metabolites(*** acid,propanoic acid,and butyric acid)and the abundance of Lactobacillus,Bifidobacterium,and Bacillus in the cecum(P<0.05).Importantly,SL significantly elevated the expression levels of jejunal zonula occludins-1(ZO-1),occluding,and fatty acid transport protein-4(FATP4)in the ETEC-challenged pigs(P<0.05).Conclusions:SL can alleviate inflammation and intestinal injury in weaned pigs upon ETEC challenge,which was associated with suppressed secretion of inflammatory cytokines and elevated serum immunoglobulins,as well as improved intestinal epithelium functions and microbiota.

关键词： Immunity Inflammation Intestinal epithelium Sialyllactose Weaned pigs

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Lentinan administration alleviates diarrhea of rotavirus-infected weaned pigs via regulating intestinal immunity

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Journal of animal Science and Biotechnology 2021年第3期12卷 1125-1137页

作者： Xiangqi Fan Haiyan Hu Daiwen Chen Bing Yu Jun He Jie Yu Junqiu Luo Erik Eckhardt Yuheng Luo Jianping Wang Hui Yan Xiangbing Mao key laboratory for animal disease-Resistance Nutrition of China Ministry of Education Key laboratory of Animal Disease-resistant Nutrition and Feed of China Ministry of Agriculture and Rural AffairsKey laboratory of Animal Disease-resistant Nutrition of Sichuan ProvinceInstitute of Animal NutritionSichuan Agricultural UniversityNo.211Gongpinghuimin RoadWenjiang DistrictChengdu 611130Sichuan ProvincePeople’s Republic of China Adisseo SAS Center of Excellence and Research in Nutrition03600 MalicorneFrance

Background:Lentinan(LNT)may regulate many important physiological functions of human and *** study aimed to verify whether LNT administration could relieve diarrhea via improving gut immunity in rotavirus(RV)-challenged weaned ***:Twenty-eight weaned pigs were randomly fed 2 diets containing 0 or 84 mg/kg LNT product for 19 d(n=14).RV infection was executed on d *** extracting polysaccharides from LNT product,its major monosaccharides were ***,LNT polysaccharide was used to administrate RV-infected IPEC-J2 ***:Dietary LNT supplementation supported normal function of piglets even when infected with RV,as reflected by reduced growth performance loss and diarrhea prevalence,and maintained gut immunity(P<0.05).The polysaccharide was isolated from LNT product,which molecular weight was 5303 Da,and major monosaccharides included glucose,arabinose and *** RV-infected IPEC-J2 cells,this polysaccharide significantly increased cell viability(P<0.05),and significantly increased anti-virus immunity via regulating pattern recognition receptors and host defense peptides(P<0.05).Conclusion:Those results suggest that LNT administration increases the piglets’resistance to RV-induced stress,likely by supporting intestinal immunity.

关键词： Gut immunity IPEC-J2 cells Lentinan Rotavirus Weaned pigs

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