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Bimetallic Single‑Atom Catalysts for Water Splitting

Nano-Micro Letters 2025年第1期17卷 1-45页

作者： Megha A.Deshmukh Aristides Bakandritsos Radek Zbořil Nanotechnology Centre Centre for Energy and Environmental TechnologiesVŠB–Technical University of Ostrava17.listopadu 2172/1570800 Ostrava‑PorubaCzech Republic Regional Centre of Advanced technologies and Materials Czech Advanced Technology and Research Institute(CATRIN)Palacky University OlomoucŠlechtitelů241/2778371 Olomouc–HoliceCzech Republic

Green hydrogen from water splitting has emerged as a critical energy vector with the potential to spearhead the global transition to a fossil fuel-independent *** field of catalysis has been revolutionized by single-atom catalysts(SACs),which exhibit unique and intricate interactions between atomically dispersed metal atoms and their ***,bimetallic SACs(bimSACs)have garnered significant attention for leveraging the synergistic functions of two metal ions coordinated on appropriately designed *** offer an avenue for rich metal–metal and metal–support cooperativity,potentially addressing current limitations of SACs in effectively furnishing transformations which involve synchronous proton–electron exchanges,substrate activation with reversible redox cycles,simultaneous multi-electron transfer,regulation of spin states,tuning of electronic properties,and cyclic transition states with low activation *** review aims to encapsulate the growing advancements in bimSACs,with an emphasis on their pivotal role in hydrogen generation via water *** subsequently delve into advanced experimental methodologies for the elaborate characterization of SACs,elucidate their electronic properties,and discuss their local coordination ***,we present comprehensive discussion on the deployment of bimSACs in both hydrogen evolution reaction and oxygen evolution reaction,the two half-reactions of the water electrolysis process.

关键词： Single-atom catalysts Single-atom dimers Hydrogen evolution Oxygen evolution Water splitting

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Epidermal growth factor receptor pathway substrate 8 (Eps8) expression in maturing testis

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Asian Journal of Andrology 2004年第3期6卷 195-203页

作者： Alain Wunsch Kai Strothmann Manuela Simoni Jorg Gromoll Eberhard Nieschlag C.Marc Luetjens institute of reproductive Medicine of the University D-48129 MunsterGermanyInstitute of Reproductive Medicine of the UniversityD-48129 MunsterGermanyInstitute of Reproductive Medicine of the UniversityD-48129 MunsterGermanyInstitute of Reproductive Medicine of the UniversityD-48129 MunsterGermanyInstitute of Reproductive Medicine of the UniversityD-48129 MunsterGermanyInstitute of Reproductive Medicine of the UniversityD-48129 MunsterGermany

Aim: Although epidermal growth factor receptors are expressed in the testes, whether they signal through epidermal growth factor receptor pathway substrate 8 (Eps8) is unknown. Here we evaluated the expression pattern of Eps8 in the maturing testis. Methods: The expression of Eps8 was analysed by Northern blotting, immunocy-tochemistry and Western blotting in primary Sertoli cell cultures and in testicular tissue of rodents. Results: Eps8 is specifically expressed in gonocytes, Leydig and Sertoli cells of the neonatal rats and in Leydig and Sertoli cells of the adult rats and mice. Although gonocytes express Eps8, no signal was found in prepubertal or mature spermatogonia and the expression level of Eps8 in Sertoli cells increases with age. No regulation of Eps8 expression in primary immature rat Sertoli cells by Follicle stimulating hormone (FSH) was detected by Western blotting. Conclusion: Eps8 seems to be involved in the growth factor-controlled regulation of cell proliferation and differentiation in the seminiferous epithelium. Eps8 is a possible marker for gonocytes and in Sertoli cells it could be involved in crosstalk with other growth factor pathways.

关键词： gene expression isoform Sertoli cell gonocyte

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Prevalence of retinopathy of prematurity in Iran： asystematic review and Meta-analysis

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International Journal of Ophthalmology(English edition) 2017年第8期10卷 1273-1279页

作者： Saman Maroufizadeh Amir A lmasi-Hashiani Reza Omani Samani Mahdi Sepidarkish Department of Epidemiology and reproductive Health Reproductive Epidemiology Research Center Royan Institute for Reproductive Biomedicine ACECR Tehran 16635-148Iran

AIM： To estimate overall prevalence of retinopathy of prematurity （ROP） in Iran using a systematic review and Meta-analysis. METHODS： A systematic review and Meta-analysis was performed of all published studies pertaining to prevalence of ROP using international and national electronic databases （ISI Web of Sciences, PubMed, Scopus, Google Scholar, SlD, Maglran, and IranMedex） from their inception until May 2016 with standard keywords. Begg and Egger tests were used to examine the publication bias and Cochran test and f statistics were used to evaluate the statistical heterogeneity. Pooled estimate of the prevalence of ROP were calculated using random effects Meta- analysis. RESULTS： The publication bias assumption was rejected by Egger tests with P-value equal to 0.024. The results of Cochran test and 12 statistics revealed substantial heterogeneity （Q=1099.02, df=25, f=97.7%, P=-0.001）. The overall prevalence of ROP using the random effect model in Iran was 26.1% （95% Ch 20.3%-31.8%）. CONCLUSION： The prevalence of ROP is relatively high in Iran. Low birth weight and gestational age are significant risk factors for the disease. Improved care, including oxygen delivery and monitoring, for preterm babies in all facility settings would reduce the number of babies affected with ROP.

关键词： retinopathy of prematurity preterm birth infant Meta-analysis systematic review Iran

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ZP2:the precision regulator of egg coat architecture during fertilization

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Science China(Life Sciences) 2024年第8期67卷 1779-1780页

作者： Yi Dong Shigang Zhao Han Zhao State Key Laboratory of reproductive Medicine and Offspring Health Center for Reproductive MedicineInstitute of WomenChildren and Reproductive HealthShandong UniversityJinan 250012China

In a recent study published in Cell,(Nishio et al.,2024)elucidated the molecular regulatory mechanism through which organisms prevent *** process intricately involves conformational changes in the zona pellucida(ZP)*** authors present a fascinating mechanism whereby post-fertilization cleavage of the ZP subunit ZP2 triggers its oligomerization,leading to crosslinking of filaments to harden the egg coat and prevent *** also systematically analyzed the pattern of ZP filament assembly,which revealed that two protofilaments comprising type I(ZP3)and type II(ZP1/ZP2/ZP4)components interlock to form a left-handed double helix *** findings are immensely beneficial for understanding the specific role of the egg coat,providing a plethora of insights.

关键词： mechanism finding crosslinking

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Optimized production of transgenic buffalo embryos and offspring by cytoplasmic zygote injection

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Journal of Animal Science and Biotechnology 2015年第4期6卷 395-401页

作者： Fanli Meng Hui Li Xiaoli Wang Guangsheng Qin Bj?rn Oback Deshun Shi Animal Reproduction institute State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresourcesGuangxi University AgResearch Ltd. Ruakura Research CentreReproductive Technologies

Background： Cytoplasmic injection of exogenous DNA into zygotes is a promising technique to generate transgenic livestock. However, it is still relatively inefficient and has not yet been demonstrated to work in buffalo. We sought to improve two key technical parameters of the procedure, namely i） how much linear DNA to inject and ii） when to inject it. For this, we introduced a constitutively expressed enhanced green fluorescent protein （EGFP） plasmid into buffalo zygotes. Results： First, we found that the proportion of EGFP-expressing blastocysts derived from zygotes injected with 20 or 50 ng/pL DNA was significantly higher than from those injected with 5 pg/mL. However, 50 ng/pL exogenous DNA compromised blastocyst development compared to non-injected IVF controls. Therefore the highest net yield of EGFP-positive blastocysts was achieved at 20 ng/pL DNA. Second, zygotes injected early （7-8 h post-insemination [hpi]） developed better than those injected at mid （12-13 hpi） or late （18-19 hpi） time points. Blastocysts derived from early injections were also more frequently EGFP-positive. As a consequence, the net yield of EGFP-expressing blastocysts was more than doubled using early vs late injections （16.4 % vs 7.7 %）. With respect to blastocyst quality, we found no significant difference in cell numbers of EGFP-positive blastocysts vs non-injected blastocysts. Following embryo transfer of six EGFP-positive blastocysts into four recipient animals, two viable buffalo calves were born. Biopsied ear tissues from both buffalo calves were analyzed for transgene presence and expression by Southern blot, PCR and confocal laser scanning microscopy, respectively. This confirmed that both calves were transgenic. Conclusions： Our cytoplasmic injection protocol improved generation of transgenic embryos and resulted in the first transgenic buffalo calves produced by this method.

关键词： Buffalo Cytoplasmic injection Plasmid Transgenic Zygote

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Testicular exosomes disturb the immunosuppressive phenotype of testicular macrophages mediated by miR-155-5p in uropathogenic Escherichia coli-induced orchitis

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Asian Journal of Andrology 2023年第3期25卷 389-397页

作者： Jia Xu Chao He Yi-Wei Fang Zhi-Yong Hu Mei-Lin Peng Yuan-Yao Chen Yu-Fang Su Chun-Yan Liu Hui-Ping Zhang Kai Zhao institute of reproductive Health Center for Reproductive MedicineTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China

Male reproductive infections are known to shape the immunological homeostasis of the testes,leading to male ***,the specific pathogenesis of these changes remains poorly *** released in the inflammatory microenvironment are important in communication between the local microenvironment and recipient ***,we aim to identify the immunomodulatory properties of inflammatory testes-derived exosomes(IT-exos)and explore their underlying mechanisms in ***-exos were isolated using a uropathogenic Escherichia coli(UPEC)-induced orchitis model and confirmed that IT-exos promoted proinflammatory M1 activation with increasing expression of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),and interleukin-6(IL-6)in *** further used small RNA sequencing to identify the differential miRNA profiles in exosomes and primary testicular macrophages(TMs)from normal and UPEC-infected testes,respectively,and identified that miR-155-5p was highly enriched in IT-exos and TMs from inflammatory *** study of bone marrow derived macrophages(BMDMs)transfected with miR-155-5p mimic showed that macrophages polarized to proinflammatory *** addition,the mice that were administrated IT-exos showed remarkable activation of TM1-like macrophages;however,IT-exos with silencing miR-155-5p showed a decrease in proinflammatory ***,we demonstrate that miR-155-5p delivered by IT-exos plays an important role in the activation of TM1 in UPEC-induced *** study provides a new perspective on the immunological mechanisms underlying inflammation-related male infertility.

关键词： male infertility miR-155-5p orchitis testicular exosome testicular macrophage polarization

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Afterword to Semen Analysis in 21st Century Medicine special issue in Asian Journal of Andrology

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Asian Journal of Andrology 2010年第1期12卷 118-123页

作者： David J. Handelsman Trevor G. Cooper reproductive Endocrinology ＆ Andrology Director ANZA C Research Institute Sydney Australia Andrology Laboratory Institute of Reproductive Medicine of the University Munster Germany

We summarise and comment on the topics discussed by the contributors to this Special Issue. As an introduction, Ann Jequier＇s [ 1 ] generally positive assessment of the new-look manual was tempered with her res- ervations about reference values, on which other chapters dwelt at length. Aspects of laboratory technique were discussed by Charlene Brazil [2], whose offering gives a rare insight into the actual workings of andrology laboratories and highlights the need for greater involvement of laboratory directors （often clinicians） in assessing the quality of data provided.

关键词： no english key word

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Foreword to Semen Analysis in 21st Century Medicine special issue in Asian Journal of Andrology

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Asian Journal of Andrology 2010年第1期12卷 7-10页

作者： David J. Handelsman Trevor G. Cooper reproductive Endocrinology ＆ Andrology ANZA C Research Institute Sydney Australia Andrology Laboratory Institute of Reproductive Medicine of the University Munster Germany

Semen analysis as an integral part of infertility investigations has a surprisingly long history, emerging only slowly, from under a cloud of disrepute and occupying a solitary niche outside conventional pathology tests, until relatively recently. From origins in the 19th century when spermatozoa were only identified as present or absent in cervical mucus samples from postcoital tests, even then a practice deemed ＂... dabbling incompatible with decency and self-respect...＂（cited in [1]）.

关键词： no english key word

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Unraveling brain aging through the lens of oral microbiota

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Neural Regeneration Research 2025年第7期20卷 1930-1943页

作者： Qinchao Hu Si Wang Weiqi Zhang Jing Qu Guang-Hui Liu State Key Laboratory of Membrane Biology Institute of ZoologyChinese Academy of SciencesBeijingChina Hospital of Stomatology Guanghua School of StomatologyGuangdong Provincial Key Laboratory of StomatologySun Yat-sen UniversityGuangzhouGuangdong ProvinceChina Advanced Innovation Center for Human Brain Protection and National Clinical Research Center for Geriatric Disorders Xuanwu HospitalCapital Medical UniversityBeijingChina Aging Translational Medicine Center International Center for Aging and CancerBeijing Municipal Geriatric Medical Research CenterXuanwu HospitalCapital Medical UniversityBeijingChina CAS Key Laboratory of Genomic and Precision Medicine Beijing Institute of GenomicsChinese Academy of Sciences and China National Center for BioinformationBeijingChina University of Chinese Academy of Sciences BeijingChina institute for Stem Cell and Regeneration Chinese Academy of SciencesBeijingChina Aging Biomarker Consortium BeijingChina State Key Laboratory of Stem Cell and reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijingChina Beijing institute for Stem Cell and Regenerative Medicine BeijingChina

The oral cavity is a complex physiological community encompassing a wide range of *** of oral microbiota can lead to various oral infectious diseases,such as periodontitis and tooth decay,and even affect systemic health,including brain aging and neurodegenerative *** studies have highlighted how oral microbes might be involved in brain aging and neurodegeneration,indicating potential avenues for intervention *** this review,we summarize clinical evidence demonstrating a link between oral microbes/oral infectious diseases and brain aging/neurodegenerative diseases,and dissect potential mechanisms by which oral microbes contribute to brain aging and *** also highlight advances in therapeutic development grounded in the realm of oral microbes,with the goal of advancing brain health and promoting healthy aging.

关键词： Alzheimer's disease brain aging multiple sclerosis neurodegeneration neurodegenerative diseases oral microbiota Parkinson's disease periodontitis bacteria Porphyromonas gingivalis

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ISOLATION AND CHARACTERIZATION OF THE SPECIFIC PEPTIDES FROM RABBIT BLASTOCYST AT PERI-IMPLANTATION STAGE WITH EMPHASIS ON THEIR PHYSIOLOGICAL SIGNIFICANCE RELEVANT TO THE MECHANISM OF IMPLANTATION

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Science China Chemistry 1988年第1期31卷 39-46页

作者：曹(讠永)清,陈幼珍,郑明,张致一 Laboratory of reproductive Biology and Department of Endocrinology institute of Zoology Academia Sinica. Beijing Laboratory of reproductive Biology and Department of Endocrinology institute of Zoology Academia Sinica. Beijing Laboratory of reproductive Biology and Department of Endocrinology institute of Zoology Academia Sinica. Beijing Laboratory of reproductive Biology and Department of Endocrinology institute of Zoology Academia Sinica. Beijing

The secrctory materials from the uterine flushings of the pregnant, non-pregnant and pseudopreg-nant rabbits were analysed by the method of SDS-polyacrylamide gel electrophoresis. The results demon-strated that there are three specific peptides in the pregnant uterine flushings with molecular weightsof 3000, 4500 and 6000. No correspondent peptides were found in non-pregnant and pseudopregnantuterine flushings. The peptides were also isolated and identified from the blastocystic fluid. At least 4peaks of small peptides were shown by high pressure liquid chromatography. The range of their isoelec-trophoretic points is between pH 4.0-5.5 Concavalin A (Con A) affinitive electrophoresis indicated thatthey are glycopeptides in nature. The LH-RH A is able to exert inhibitory effects on the synthesis ofthese peptides. Their possible physiological function has been discussed.

关键词： ISOLATION AND CHARACTERIZATION OF THE SPECIFIC PEPTIDES FROM RABBIT BLASTOCYST AT PERI-IMPLANTATION STAGE WITH EMPHASIS ON THEIR PHYSIOLOGICAL SIGNIFICANCE RELEVANT TO THE MECHANISM OF IMPLANTATION

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