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Update on thyroid ultrasound: a narrative review from diagnostic criteria to artificial intelligence techniques

Chinese Medical Journal 2019年第16期132卷 1974-1982页

作者： Xiao-Wen Liang Yong-Yi Cai Jin-Sui Yu Jian-Yi Liao Zhi-Yi Chen Department of Ultrasound Medicine Laboratory of Ultrasound Medicine and Artificial IntelligenceExperimental Center of Liwan HospitalThe Third Affiliated Hospital of Guangzhou Medical UniversityThe Liwan Hospital of the Third Affiliated Hospital of Guangzhou Medical UniversityGuangzhouGuangdong 510000China.

Objective Ultrasound imaging is well known to play an important role in the detection of thyroid disease, but the management of thyroid ultrasound remains inconsistent. Both standardized diagnostic criteria and new ultrasound technologies are essential for improving the accuracy of thyroid ultrasound. This study reviewed the global guidelines of thyroid ultrasound and analyzed their common characteristics for basic clinical screening. Advances in the application of a combination of thyroid ultrasound and artificial intelligence (AI) were also presented. Data sources An extensive search of the PubMed database was undertaken, focusing on research published after 2001 with keywords including thyroid ultrasound, guideline, AI, segmentation, image classification, and deep learning. Study selection Several types of articles, including original studies and literature reviews, were identified and reviewed to summarize the importance of standardization and new technology in thyroid ultrasound diagnosis. Results Ultrasound has become an important diagnostic technique in thyroid nodules. Both standardized diagnostic criteria and new ultrasound technologies are essential for improving the accuracy of thyroid ultrasound. In the standardization, since there are no global consensus exists, common characteristics such as a multi-feature diagnosis, the performance of lymph nodes, explicit indications of fine needle aspiration, and the diagnosis of special populations should be focused on. Besides, evidence suggests that AI technique has a good effect on the unavoidable limitations of traditional ultrasound, and the combination of diagnostic criteria and AI may lead to a great promotion in thyroid diagnosis. Conclusion Standardization and development of novel techniques are key factors to improving thyroid ultrasound, and both should be considered in normal clinical use.

关键词： Thyroid Ultrasound Guideline Artificial Intelligence Image Classification

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Towards precision medicine:Omics approach for COVID-19

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Biosafety and Health 2023年第2期5卷 78-88页

作者： Xiaoping Cen Fengao Wang Xinhe Huang Dragomirka Jovic Fred Dubee Huanming Yang Yixue Li College of Life Sciences University of Chinese Academy of SciencesBeijing 100049China guangzhou laboratory No.9 XingDaoHuanBei RoadGuangzhou International Bio IslandGuangzhou 510005China BGI-Shenzhen Shenzhen 518083China IBMC-BGI Center the Cancer Hospital of the University of Chinese Academy of Sciences(Zhejiang Cancer Hospital)Institute of Basic Medicine and Cancer(IBMC)Chinese Academy of SciencesHangzhou 310022China key laboratory of Systems Health Science of Zhejiang Province School of Life ScienceHangzhou Institute for Advanced StudyUniversity of Chinese Academy of SciencesHangzhou 310024China key laboratory of molecular Biophysics of Ministry of Education Hubei Bioinformatics and Molecular Imaging Key LaboratoryCenter for Artificial Intelligence BiologyCollege of Life Science and TechnologyHuazhong University of Science and TechnologyWuhan 430074China Lars Bolund Institute of Regenerative Medicine Qingdao-Europe Advanced Institute for Life SciencesBGI-QingdaoQingdao 266555China GZMU-GIBH Joint School of Life Sciences The Guangdong-Hong Kong-MacaoJoint Laboratory for Cell Fate Regulation and DiseasesGuangzhou Medical UniversityGuangzhou 511436China School of Life Sciences and Biotechnology Shanghai Jiao Tong UniversityShanghai 200240China Shanghai Institute of Nutrition and Health Chinese Academy of Sciences ShanghaiShanghai 200030China Collaborative Innovation Center for Genetics and Development Fudan UniversityShanghai 200433China Shanghai Institute for Biomedical and Pharmaceutical Technologies Shanghai 200032China

The coronavirus disease 2019(COVID-19)pandemic had a devastating impact on human *** with genome surveillance of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),the development of omics technologies brought a clearer understanding of the complex SARS-CoV-2 and ***,we reviewed how omics,including genomics,proteomics,single-cell multi-omics,and clinical phenomics,play roles in answering biological and clinical questions about ***-scale sequencing and advanced analysis methods facilitate COVID-19 discovery from virus evolution and severity risk prediction to potential treatment *** would indicate precise and globalized prevention and medicine for the COVID-19 pandemic under the utilization of big data capability and phenotypes ***,decoding the evolution rule of SARS-CoV-2 by deep learning models is promising to forecast new variants and achieve more precise data to predict future pandemics and prevent them on time.

关键词： COVID-19 Artificial intelligence Multi-omics Precision medicine

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Dose optimization of intrathecal administration of human umbilical cord mesenchymal stem cells for the treatment of subacute incomplete spinal cord injury

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Neural Regeneration Research 2022年第8期17卷 1785-1794页

作者： Ting-Ting Cao Huan Chen Mao Pang Si-Si Xu Hui-Quan Wen Bin Liu Li-Min Rong Mang-Mang Li Department of Cell Biology School of Basic Medical SciencesSouthern Medical UniversityGuangzhouGuangdong ProvinceChina Department of Spine Surgery The Third Affiliated Hospital of Sun Yat-sen UniversityGuangzhouGuangdong ProvinceChina Guangdong Provincial Center for Engineering and Technology Research of Minimally Invasive Spine Surgery GuangzhouGuangdong ProvinceChina Guangdong Provincial Center for Quality Control of Minimally Invasive Spine Surgery GuangzhouGuangdong ProvinceChina Department of Radiology The Third Affiliated Hospital of Sun Yat-sen UniversityGuangzhouGuangdong ProvinceChina

Human umbilical cord mesenchymal stem cells(hUC-MSCs)are a promising candidate for spinal cord injury(SCI)repair owing to their advantages of low immunogenicity and easy accessibility over other MSC ***,modest clinical efficacy hampered the progression of these cells to clinical *** discrepancy may be due to many variables,such as cell source,timing of implantation,route of administration,and relevant efficacious cell dose,which are critical factors that affect the efficacy of treatment of patients with ***,we have evaluated the safety and efficacy of 4×10^(6) hUC-MSCs/kg in the treatment of subacute SCI by intrathecal implantation in rat *** search for a more accurate dose range for clinical translation,we compared the effects of three different doses of hUC-MSCs-low(0.25×10^(6) cells/kg),medium(1×10^(6) cells/kg)and high(4×10^(6) cells/kg)-on subacute SCI repair through an elaborate combination of behavioral analyses,anatomical analyses,magnetic resonance imaging-diffusion tensor imaging(MRI-DTI),biotinylated dextran amine(BDA)tracing,electrophysiology,and quantification of mRNA levels of ion channels and neurotransmitter *** study demonstrated that the medium dose,but not the low dose,is as efficient as the high dose in producing the desired therapeutic ***,partial restoration of theγ-aminobutyric acid type A(GABAA)receptor expression by the effective doses indicates that GABAA receptors are possible candidates for therapeutic targeting of dormant relay pathways in injured spinal ***,this study revealed that intrathecal implantation of 1×10^(6) hUC-MSCs/kg is an alternative approach for treating subacute SCI.

关键词： effective dose human umbilical cord mesenchymal stem cells intrathecal implantation ion channels neurotransmitter receptors spinal cord injury subacute spinal cord injury γ-aminobutyric acid type A(GABA_(A))receptors

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Time-lapse changes of in vivo injured neuronal substructures in the central nervous system after low energy two-photon nanosurgery

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Neural Regeneration Research 2017年第5期12卷 751-756页

作者： Zhikai Zhao Shuangxi Chen Yunhao Luo Jing Li Smaranda Badea Chaoran Ren Wutian Wu Guangdong-Hong Kong-Macao Institute of CNS Regeneration Jinan University Guangzhou Guangdong Province China School of Biomedical Sciences Division of Anatomy The University of Hong Kong Pokfulam Hong Kong Special Administrative Region China Guangdong Engineering Research Center of Stem Cell Storage and clinical Application Saliai Stem Cell Science and Technology Guangzhou Guangdong Province China State key laboratory of Brain and Cognitive Sciences The University of Hong Kong Pokfulam Hong Kong Special Administrative Region China

There is currently very little research regarding the dynamics of the subcellular degenerative events that occur in the central nervous system in response to injury. To date, multi-photon excitation has been primarily used for imaging applications; however, it has been recently used to selectively disrupt neural structures in living animals. However, understanding the complicated processes and the essential underlying molecular pathways involved in these dynamic events is necessary for studying the underlying process that promotes neuronal regeneration. In this study, we introduced a novel method allowing in vivo use of low energy（less than 30 m W） two-photon nanosurgery to selectively disrupt individual dendrites, axons, and dendritic spines in the murine brain and spinal cord to accurately monitor the time-lapse changes in the injured neuronal structures. Individual axons, dendrites, and dendritic spines in the brain and spinal cord were successfully ablated and in vivo imaging revealed the time-lapse alterations in these structures in response to the two-photon nanosurgery induced lesion. The energy（less than 30 m W） used in this study was very low and caused no observable additional damage in the neuronal sub-structures that occur frequently, especially in dendritic spines, with current commonly used methods using high energy levels. In addition, our approach includes the option of monitoring the time-varying dynamics to control the degree of lesion. The method presented here may be used to provide new insight into the growth of axons and dendrites in response to acute injury.

关键词： nerve regeneration dendrite dendritic spine axon spinal cord two-photon nanosurgery single-synapse resolution neural regeneration

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The application of FAPI-targeted theranostics in pancreatic cancer:a narrative review

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Journal of Pancreatology 2022年第2期5卷 78-86页

作者： Meixi Liu Marcus Hacker Li Huo Xiang Li The Nuclear Medicine Department State Key Laboratory of Complex Severe and Rare DiseasesCenter for Rare Diseases ResearchBeijing Key Laboratory of Molecular Targeted Diagnosis and Therapy in Nuclear MedicinePeking Union Medical College HospitalChinese Academy of Medical Science and Peking Union Medical CollegeBeijingChina Division of Nuclear Medicine Department of Biomedical Imaging and Image-Guided TherapyMedical University of ViennaViennaAustria

Pancreatic cancer is one of the most lethal malignancies in the ***-associated fibroblasts are one of the main components of tumor microenvironment in pancreatic cancer and play an essential role in tumor *** activation protein that is expressed in specific subtypes of cancer-associated fibroblasts promotes tumor growth and is related to poor *** researches have preliminarily demonstrated a promising potential of radiopharmaceuticals targeting fibroblast activation protein in diagnosis and therapy of pancreatic *** article comprehensively reviews the current development and clinical translation of fibroblast activation protein inhibitor-targeting radiopharmaceuticals in pancreatic cancer and provides significant perspectives for future investigations.

关键词： Diagnostic imaging Fibroblast activation protein Fibroblast activation protein inhibitor Pancreatic cancer Radiopharmaceuticals Theranostics

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A DNA tetrahedron-based nanosuit for efficient delivery of amifostine and multi-organ radioprotection

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Bioactive Materials 2024年第9期39卷 191-205页

作者： Yuting Yang Jinlong Yang Jianwei Zhu Xingyu Chen Li Zhou Wenjuan Ma Yunfeng Lin State key laboratory of Oral Diseases National Center for StomatologyNational Clinical Research Center for Oral DiseasesWest China Hospital of StomatologySichuan UniversityChengduSichuan610041PR China Sichuan Provincial Engineering Research Center of Oral Biomaterials ChengduSichuan610041PR China National Center for translational Medicine Shanghai Jiao Tong UniversityShanghai200240PR China Department of Neurosurgery of West China Hospital Sichuan UniversityChengdu610041PR China Department of Neurosurgery Sichuan Provincial People’s HospitalUniversity of Electronic Science and Technology of Chin1Chengdu611731PR China Histology and imaging Platform Core Facility of West China HospitalSichuan UniversityChengdu610041PR China

Unnecessary exposure to ionizing radiation(IR)often causes acute and chronic oxidative damages to normal cells and organs,leading to serious physiological and even life-threatening ***(AMF)is a validated radioprotectant extensively applied in radiation and chemotherapy medicine,but the short half-life limits its bioavailability and clinical applications,remaining as a great challenge to be *** nanostructures especially the tetrahedral framework nucleic acids(tFNAs)are promising nanocarriers with preeminent biosafety,low biotoxicity,and high transport *** tFNAs also have a relative long-term maintenance for structural stability and excellent endocytosis *** therefore synthesized a tFNA-based delivery system of AMF for multi-organ radioprotection(tFNAs@AMF,also termed nanosuit).By establishing the mice models of accidental total body irradiation(TBI)and radiotherapy model of Lewis lung cancer,we demonstrated that the nanosuit could shield normal cells from IR-induced DNA damage by regulating the molecular biomarkers of anti-apoptosis and anti-oxidative *** the accidental total body irradiation(TBI)mice model,the nanosuit pretreated mice exhibited satisfactory alteration of superoxide dismutase(SOD)activities and malondialdehyde(MDA)contents,and functional recovery of hematopoietic system,reducing IRinduced pathological damages of multi-organ and safeguarding mice from lethal *** importantly,the nanosuit showed a selective radioprotection of the normal organs without interferences of tumor control in the radiotherapy model of Lewis lung *** on a conveniently available DNA tetrahedron-based nanocarrier,this work presents a high-efficiency delivery system of AMF with the prolonged half-life and enhanced radioprotection for *** nanosuit pioneers a promising strategy with great clinical translation potential for radioactivity protection.

关键词： Ionizing radiation Nanosuit Amifostine Tetrahedral framework nucleic acids DNA damage Multi-organ protection Lewis lung cancer model

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Intelligent nanomaterials for cancer therapy:recent progresses and future possibilities

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Medical Review 2023年第4期3卷 321-342页

作者： Jing Wang Yuliang Zhao Guangjun Nie CAS key laboratory for Biomedical Effects of Nanomaterials and Nanosafety CAS Center of Excellence in NanoscienceNational Center for Nanoscience and TechnologyBeijingChina Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijingChina CAS key laboratory for Biomedical Effects of Nanomaterials and Nanosafety CAS Center of Excellence in NanoscienceNational Center for Nanoscience and TechnologyBeijing 100190China GBA Research Innovation Institute for Nanotechnology Guangzhou 510700Guangdong ProvinceChina Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing 100049China

Intelligent nanomedicine is currently one of the most active frontiers in cancer therapy *** by the recent progresses of nanobiotechnology,a new generation of multifunctional nanotherapeutics and imaging platforms has remarkably improved our capability to cope with the highly heterogeneous and complicated na-ture of *** rationally designed multifunctionality and programmable assembly of functional subunits,the in vivo behaviors of intelligent nanosystems have become increasingly tunable,making them more efficient in performing sophisticated actions in physiological and path-ological *** recent years,intelligent nanomaterial-based theranostic platforms have showed great potential in tumor-targeted delivery,biological barrier circumvention,multi-responsive tumor sensing and drug release,as well as convergence with precise medication approaches such as personalized tumor *** the other hand,the increasing system complexity of anti-cancer nanomedicines also pose signiﬁcant challenges in charac-terization,monitoring and clinical use,requesting a more comprehensive and dynamic understanding of nano-bio *** review aims to brieﬂy summarize the recent progresses achieved by intelligent nanomaterials in tumor-targeted drug delivery,tumor immunotherapy and temporospatially speciﬁc tumor imaging,as well as impor-tant advances of our knowledge on their interaction with biological *** the perspective of clinical translation,we have further discussed the major possibilities provided by disease-oriented development of anti-cancer nano-materials,highlighting the critical importance clinically-oriented system design.

关键词： intelligent nanomedicine anti-cancer nano-materials tumor microenvironment tumor vaccines immunotherapy personalized medication

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PHF6 functions as a tumor suppressor by recruiting methyltransferase SUV39H1 to nucleolar region and offers a novel therapeutic target for PHF6-muntant leukemia

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Acta Pharmaceutica Sinica B 2022年第4期12卷 1913-1927页

作者： Hsiang-i Tsai Yanping Wu Rui Huang Dandan Su Yingyi Wu Xiaoyan Liu Linglu Wang Zhanxue Xu Yuxin Pang Chong Sun Chao He Fan Shu Haitao Zhu Dongqing Wang Fang Cheng Laiqiang Huang Hongbo Chen School of Pharmaceutical Sciences(Shenzhen) Sun Yat-sen UniversityShenzhen 518107China Department of Medical imaging the Affiliated Hospital of Jiangsu UniversityZhenjiang 212001China Department of Hematology Zhujiang HospitalSouthern Medical UniversityGuangzhou 510000China The Shenzhen key Lab of Gene and Antibody Therapy Center for Biotechnology&BiomedicineDivision of Life and Health SciencesGraduate School at ShenzhenTsinghua UniversityShenzhen 518055China School of Traditional Medicine Materials Resource Guangdong Pharmaceutical UniversityYunfu 527322China Immune Regulation in Cancer German Cancer Research CenterHeidelberg 69120Germany

Mutations in the plant homeodomain-like finger protein 6(PHF6)gene are strongly associated with acute myeloid(AML)and T-cell acute lymphoblastic leukemia(T-ALL).In this study,we demonstrated that PHF6 can bind to H3K9me3 and H3K27me1 on the nucleolar chromatin and recruit histone methyltransferase SUV39H1 to the rDNA *** deletion of PHF6 caused a decrease in the recruitment of SUV39H1 to rDNA gene loci,resulting in a reduction in the level of H3K9me3 and the promotion of rDNA *** knockdown of either SUV39H1 or PHF6 significantly attenuated the effects of increase in H3K9me3 and suppressed the transcription of rDNA induced by the overexpression of the other interacting partner,thereby establishing an interdependent relationship between PHF6 and SUV39H1 in their control of rRNA *** PHF6 clinical mutants significantly impaired the ability to bind and recruit SUV39H1 to the rDNA loci,resulting in an increase in rDNA transcription activity,the proliferation of in vitro leukemia cells,and the growth of in vivo mouse ***,significantly elevated levels of pre-rRNA were observed in clinical AML patients who possessed a mutated version of *** specific rDNA transcription inhibitor CX5461 significantly reduced the resistance of U937 AML cells deficient in PHF6 to cytarabine,the drug that is most commonly used to treat ***,we revealed a novel molecular mechanism by which PHF6 recruits methyltransferase SUV39H1 to the nucleolar region in leukemia and provided a potential therapeutic target for PHF6-mutant leukemia.

关键词： PHF6 SUV39H1 AML rDNA transcription Epigenetic CX5461 Methyltransferase Leukemia

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Preserving cognitive function in patients with Alzheimer's disease:The Alzheimer's disease neuroprotection research initiative(ADNRI)

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Neuroprotection 2023年第2期1卷 84-98页

作者： Jie Liu Heleen van Beusekom Xian-Le Bu Gong Chen Paulo Henrique Rosado de Castro Xiaochun Chen Xiaowei Chen Andrew N.Clarkson Tracy D.Farr Yuhong Fu Jianping Jia Jukka Jolkkonen Woojin Scott Kim Paula Korhonen Shen Li Yajie Liang Guang-Hui Liu Guiyou Liu Yu-Hui Liu Tarja Malm Xiaobo Mao Joaquim Miguel Oliveira Mike M.Modo Pedro Ramos-Cabrer Karsten Ruscher Weihong Song Jun Wang Xuanyue Wang Yun Wang Haitao Wu Lize Xiong Yi Yang Keqiang Ye Jin-Tai Yu Xin-Fu Zhou Marietta Zille Colin L.Masters Piotr Walczak Boltze Johannes Xunming Ji Yan-Jiang Wang Department of Neurology Daping HospitalThird Military Medical UniversityChongqingChina Chongqing key laboratory of Ageing and Brain Diseases ChongqingChina Division of Experimental Cardiology Department of CardiologyErasmus MCUniversity Medical CenterRotterdamThe Netherlands Institute of Brain and Intelligence Third Military Medical UniversityChongqingChina Guangdong-HongKong-Macao Institute of CNS Regeneration(GHMICR) Jinan UniversityGuangzhouGuangdongChina Institute of Biomedical Sciences Federal University of Rio de JaneiroRio de JaneiroRio de JaneiroBrazil Fujian key laboratory of molecular Neurology Department of Neurology and GeriatricsFujian Institute of GeriatricsFujian Medical University Union HospitalInstitute of NeuroscienceFujian Medical UniversityFuzhouFujianChina Guangyang Bay laboratory Chongqing Institute for Brain and IntelligenceChongqingChina Center for Excellence in Brain Science and Intelligence Technology Chinese Academy of SciencesShanghaiChina Department of Anatomy Brain Health Research Centre and Brain Research New ZealandUniversity of OtagoDunedinNew Zealand School of Life Sciences University of NottinghamNottinghamUK Brain and Mind Centre&School of Medical Sciences The University of SydneySydneyNew South WalesAustralia Innovation Center for Neurological Disorders and Department of Neurology Xuanwu HospitalNational Clinical Research Center for Geriatric DiseasesCapital Medical UniversityBeijingChina A.I.Virtanen Institute for molecular Sciences University of Eastern FinlandKuopioFinland Department of Neurology and Psychiatry Beijing Shijitan HospitalCapital Medical UniversityBeijingChina Department of Diagnostic Radiology and Nuclear Medicine University of Maryland School of MedicineBaltimoreMarylandUSA University of Chinese Academy of Sciences BeijingChina State key laboratory of Membrane Biology Institute of ZoologyChinese Academy of SciencesBeijingChina Beijing Institute of Brain Disorders Laboratory of Brain DisordersMinistry of Sci

The global trend toward aging populations has resulted in an increase in the occurrence of Alzheimer's disease(AD)and associated socioeconomic *** metabolism of amyloid-β(Aβ)has been proposed as a significant pathomechanism in AD,supported by results of recent clinical trials using anti-Aβ***,the cognitive benefits of the current treatments are *** etiology of AD is multifactorial,encompassing Aβand tau accumulation,neuroinflammation,demyelination,vascular dysfunction,and comorbidities,which collectively lead to widespread neurodegeneration in the brain and cognitive ***,solely removing Aβfrom the brain may be insufficient to combat neurodegeneration and preserve *** attain effective treatment for AD,it is necessary to(1)conduct extensive research on various mechanisms that cause neurodegeneration,including advances in neuroimaging techniques for earlier detection and a more precise characterization of molecular events at scales ranging from cellular to the full system level;(2)identify neuroprotective intervention targets against different neurodegeneration mechanisms;and(3)discover novel and optimal combinations of neuroprotective intervention strategies to maintain cognitive function in AD *** Alzheimer's Disease Neuroprotection Research Initiative's objective is to facilitate coordinated,multidisciplinary efforts to develop systemic neuroprotective strategies to combat *** aim is to achieve mitigation of the full spectrum of pathological processes underlying AD,with the goal of halting or even reversing cognitive decline.

关键词： Alzheimer's disease early intervention neural regeneration neuroprotection systematic perspective

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Tn5-FISH,a novel cytogenetic method to image chromatin interactions with sub-kilobase resolution

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Journal of Genetics and Genomics 2020年第12期47卷 727-734页

作者： Jing Niu Xu Zhang Li Guipeng Pixi Yan Qing Yan Qionghai Dai Dayong Jin Xiaohua Shen Jichang Wang Michael QZhang Juntao Gao School of Medicine Tsinghua UniversityBeijing 100084China Department of Automation Tsinghua UniversityBeijing 100084China Beijing Institute of Collaborative Innovation Beijing 100094China Medi-X Institute SUSTech Academy for Advanced Interdisciplinary StudiesSouthern University of Science and TechnologyShenzhen 518055China Department of Biology Southern University of Science and TechnologyShenzhen 518055China MOE key laboratory of Bioinformatics Bioinformatics DivisionBNRistCenter for Synthetic&Systems BiologyTsinghua UniversityBeijing 100084China Institute for Biomedical Materials and Devices(IBMD) Faculty of ScienceUniversity of Technology SydneyNSW 2007Australia UTS-SUStech Joint Research Centre for Biomedical Materials and Devices Department of Biomedical EngineeringSouthern University of Science and TechnologyShenzhen 518055China key laboratory for Stem Cells and Tissue Engineering(Sun Yat-sen University) Ministry of EducationGuangzhou 510080China Department of histology and embryology Zhongshan School of MedicineSun Yat-sen UniversityGuangzhou 510080China Department of Biological Sciences Center for Systems BiologyThe University of TexasDallasTX 75080-3021USA

There is an increasing interest in understanding how three-dimensional(3D)organization of the genome is *** strategies have been employed to identify genome-wide chromatin ***,due to current limitations in resolving genomic contacts,visualization and validation of these genomic loci with sub-kilobase resolution remain unsolved to ***,we describe Tn5 transposase-based Fluorescencein situhybridization(Tn5-FISH),a PCR-based,cost-effective imaging method,which can co-localize the genomic loci with sub-kilobase resolution,dissect genome architecture,and verify chromatin interactions detected by chromatin configuration capture(3C)-derived *** validate this method,short-range interactions in keratin-encoding gene(KRT)locus in topologically associated domain(TAD)were imaged by triple-color Tn5-FISH,indicating that Tn5-FISH is very useful to verify short-range chromatin interactions inside the contact domain and ***,Tn5-FISH can be a powerful molecular tool for the clinical detection of cytogenetic changes in numerous genetic diseases such as cancers.

关键词： Fluorescencein situhybridization Chromatin interaction Cellular imaging Super resolution

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