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The mRNA Expression Profiles of Five Heat Shock Protein Genes from Frankliniella occidentalis at Different Stages and Their Responses to Temperatures and Insecticides

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Journal of Integrative Agriculture 2014年第10期13卷 2196-2210页

作者： WANG Hai-hong Stuart R Reitz WANG Li-xia WANG Shuai-yu LI Xue LEI Zhong-ren State Key Laboratory for Biology of plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural Sciences Malheur County Extension Oregon State University the general station of plant protection in shandong

The westem flower thrips, Frankliniella occidental＆（Pergande） is a highly invasive pest that is able to exploit many crops across a wide range of environmental conditions. Five full-length cDNAs of heat shock protein （HSP） genes （Fo-HSP90, Fo-HSP70, Fo-HSP60, Fo-HSP40 and Fo-HSP28.9） were cloned from F. occidentalis, and their expression profiles were investigated under conditions of thermal stress and insecticide exposure, and at different stages during development, using real-time quantitative PCR. All five gene sequences showed high similarity to homologs in other species, indicating the conserved fimction of this gene family. HSP60 represents an informative phylogenetic marker at the ordinal taxonomic level within Insecta, but HSP90, which has two homologous copies in Hymenoptera, was not informative. The expression of Fo-HSPs under thermal stress suggests that Fo-HSP90, Fo-HSP70, and Fo-HSP28.9 are inducible by both cold and heat stress, Fo-HSP40 is only heat-inducible, and Fo-HSP60 is thermally insensitive. There were two patterns of cold induction of Fo-HSPs： one is from 0 to 4℃ and the other is around -8℃. All five Fo-HSPs genes were induced by exposure to sublethal concentrations of the insecticide avermectin. The expression of the five Fo-HSPs during different developmental stages suggests that they all play a role in development of F. occidentalis.

关键词： Frankliniella occidentalis heat shock protein genes environmental stress development expression profile

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Influence of Host Shift on Genetic Differentiation of the Oriental Fruit Fly,Bactrocera dorsalis

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Journal of Integrative Agriculture 2014年第12期13卷 2701-2708页

作者： WAN Xuan-wu LIU Ying-hong LUO Lin-ming FENG Chuan-hong WANG Sheng MA Li College of plant protection Southwest University/Chongqing Key Laboratory of Entomology and Pest Control Engineering Sichuan plant protection station

Invasion of the oriental fruit lfy, Bactrocera dorsalis, into new niches containing different food sources （a process referred to as host shift）, may cause population genetic differentiation and sympatric speciation. To attempt to infer that experimentally, test populations were established by transferring a subset of the original populations, which had been grown on banana for many generations, onto navel orange, and then subculturing the navel orange population and banana population for at least 20 generations. Four pairs of SSR primers with high polymorphism on laboratory strains were used to detect population genetic differentiation. All six tested populations （the 5th, 10th and 15th generations of B. dorsalis fed on banana and navel orange, respectively） were found to have low genetic diversity. Furthermore, the genetic diversity of the navel orange populations was found to decline after being crossed for several generations. Populations initially were deviated from Hardy-Weinberg equilibrium, however, equilibrium was achieved with increasing numbers of generations in both of the host populations. Limited gene lfows were found among the six populations. The Nei’s standard genetic distances between the two host populations of the same generation were initially low, but increased with generation number. Genetic distances between banana and navel orange populations of the same generation were lower than genetic distances between different generations grown on the same host plant. Analysis of molecular distance （AMOVA） results based on generation groups and host groups demonstrated that genetic variation among generations was greater than that between the two host populations. The results indicated that population genetic differentiation occurred after the host shift, albeit at low level. Biogeography and taxonomy of the B. dorsalis complex revealed that speciation of B. dorsalis might be tightly associated with host shift or host specialization of B. dorsalis foll

关键词： Bactrocera dorsalis oriental fruit lfy host shift population genetic differentiation sympatric speciation

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Assessment of potential dermal and inhalation exposure of workers to the insecticide imidacloprid using whole-body dosimetry in China

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Journal of Environmental Sciences 2015年第1期27卷 139-146页

作者： Lidong Cao Bo Chen Li Zheng Dongwei Wang Feng Liu Qiliang Huang Institute of plant protection Chinese Academy of Agricultural Sciences College of plant protection Shandong Agricultural University School of Chemistry and Chemical Engineering Nanjing University

In China, although improvements to the pesticide registration process have been made in la thirty years, no occupational exposure data are required to obtain a commercial license for pesticide product. Consequently, notably little research has been conducted to establish a exposure assessment procedure in China. The present study monitored the potential derm operator exposure from knapsack electric sprayer wheat field application of imidacloprid Liaocheng City, shandong Province and in Xinxiang City, Henan Province, China, usin whole-body dosimetry. The potential inhalation exposure was determined using a person air pump and XAD-2 sample tubes. The analytical method was developed and validate including such performance parameters as limits of detection and quantification, linear rang recovery and precision. The total potential dermal and inhalation exposures were 14.20, 16.815.39 and 20.78 mL/hr, respectively, for the four operators in Liaocheng and Xinxian corresponding to 0.02% to 0.03% of the applied volume of spray solution. In all trials, the low part（thigh, lower leg） of the body was the most contaminated, accounting for approximate76% to 88% of the total exposure. The inhalation exposure was less than 1% of the tot exposure. Such factors as the application pattern, crop type, spray equipment, operat experience and climatic conditions have been used to explain the exposure distribution ov the different parts of the body. As indicated by the calculated Margin of Exposure, the typic wheat treatment scenarios when a backpack sprayer was used are considered to be safe terms of imidacloprid exposure.

关键词： Potential dermal and inhalation exposure Insecticide imidacloprid Whole body dosimetry Margin of exposure China

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Effect of cDNA fragments in different length derived from Potato Virus Y coat protein gene on the induction of RNA-mediated virus resistance

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Science China(Life Sciences) 2004年第4期47卷 382-388页

作者： ZHU Junhua ZHU Xiaoping WEN Fujiang BAI Qingrong ZHU Changxiang SONG Yunzhi Laboratory of plant Genetic Engineering College of Plant ProtectionShandong Agricultural UniversityTaian 271018China

We have reported that cDNA derived from entire coat protein (CP) gene of potato virus Y (PVY) could induce resistance to PVY infection in transgenic tobacco plants, and the resistance was further demonstrated to be RNA-mediated rather than coat protein-mediated. In this study, we cloned cDNA fragments of 202 bp, 417 bp, and 603 bp in length derived from the 3′ end of the PVY CP gene, and the cDNA fragments were introduced into tobacco (var. NC89) plants via Agrobacterium-mediated transformation system. The results of resistance assay showed that the CP cDNA fragments of 417 bp and 603 bp could confer resistance of the trans-genic plants to PVY infection, but the fragment of 202 bp in length could not. Molecular analysis revealed that the resistance was RNA-mediated, which is believed to be a result of post-transcriptional gene silencing. The results indicate that the length of cDNA fragments needed for resistance induction was located somewhere between 202 bp and 417 bp from the 3′ end of PVY CP gene.

关键词： PVY partial length of CP gene fragments transgenic tobacco plants RNA-mediated resistance post-transcrip-tional gene silencing.

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Molecular Taxonomy of Conogethes punctiferalis and Conogethes pinicolalis(Lepidoptera: Crambidae) Based on Mitochondrial DNA Sequences

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Journal of Integrative Agriculture 2014年第9期13卷 1982-1989页

作者： WANG Jing ZHANG Tian-tao WANG Zhen-ying HE Kang-lai LIU Yong LI Jing State Key Laboratory for Biology of the plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences College of plant protection Shandong Agricultural University

Conogethes punctiferalis（Guenée）（Lepidoptera： Crambidae） was originally considered as one species with fruit-feeding type（FFT） and pinaceae-feeding type（PFT）, but it has subsequently been divided into two different species of Conogethes punctiferalis and Conogethes pinicolalis. The relationship between the two species was investigated by phylogenetic reconstruction using maximum-likelihood（ML） parameter estimations. The phylogenetic tree and network were constructed based upon sequence data from concatenation of three genes of mitochondrial cytochrome c oxidase subunits I, II and cytochrome b which were derived from 118 samples of C. punctiferalis and 24 samples of C. pinicolalis. The phylogenetic tree and network showed that conspecific sequences were clustering together despite intraspecific variability. Here we report the results of a combined analysis of mitochondrial DNA sequences from three genes and morphological data representing powerful evidence that C. pinicolalisand C. punctiferalis are significantly different.

关键词： yellow peach moth Conogethes punctiferalis Conogethes pinicolalis mitochondrial DNA sequence

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Identification and Molecular Characterization of a Phytoplasma Associated with Pomegranate Fasciation Disease

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Horticultural plant Journal 2018年第1期4卷 30-34页

作者： GAOdb Rui WANG Jie ZHU Tiansheng JIA Xi LI Xiangdong Institute of plant protection Shandong Academy of Agricultural Sciences Department of plant Pathology College of Plant Protection Shandong Agricultural University shandong Institute of Pomology College of plant science and Technology Tarim University Institute of Maize Shandong Academy of Agricultural Sciences

To confirm phytoplasma infection,samples of pomegranate(Punica granatum L.)plants showing symptoms of fasciation were collected from an orchard located in Tai’an,shandong Province,China.A fragment of approximately 1.2 kb was amplified with universal primers targeting the phytoplasma 16S r RNA gene from symptomatic pomegranate plants,while no fragment was obtained from healthy *** phytoplasma associated with the disease was designated as pomegranate fasciation(Po F).Two representative phytoplasma 16S r DNA gene sequences(Po F-Ch01 and Po F-Ch02)had 100%nucleotide sequence *** 16S r DNA sequence of Po F-Ch01 and Po F-Ch02 showed the highest similarity(99.6%)to that of‘***’phytoplasma isolate AY-PG,which belong to 16Sr *** phylogenetic analysis showed that Po F-Ch01 and Po F-Ch02 belonged to a cluster of 16Sr I subgroup *** silico RFLP analysis indicated that Po F-Ch01 shared the highest similarity coefficient of 0.97 with reference strains of 16Sr I-B,M and *** RFLP analysis of both enzymes Bst U I and Bfa I confirmed that of the virtual RFLP *** these results,we concluded that Po F was a member of the‘Candidatus Phytoplasma asteris’group(16Sr I),and has very close relationship with 16Sr I-B subgroup.

关键词： Punica granatum fasciation disease RFLP analysis 16Sr I group

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Effects of humic acid coatings on phenanthrene sorption to black carbon

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Journal of Environmental Sciences 2007年第10期19卷 1189-1192页

作者： WU Cheng ZHANG Xiao-li LI Guan-bin School of Chemistry and Chemical Engineering Shandong University Jinan 250100 China shandong Agro-Environment protection station Jinan 250100 China

Black carbon （BC） can strongly adsorb hydrophobic organic compounds （HOCs）. The HOC sorption to coated BC could be attenuated in soil and sediment compared with that of the parent BC. To study the potential causes of the sorption attenuation, humic acid （HA） and BC were isolated. Phenanthrene （PHE） was selected as the representative of HOCs. BC was coated with the precipitated HA. The PHE sorption to the HA-coated BC was determined. The HA coatings on BC could result in the significant sorption attenuation of PHE to BC. The attenuation varied in different HA origin and was positively correlated to the aromaticity of HA. The attenuation could be explained by the direct competition between HA and PHE for the available sorption sites on BC and the reduction of the available sorption sites as a result of the pore blockage of BC caused by the HA coatings. Therefore, the HA coatings on BC was one potential cause of the attenuation of HOC sorption to BC in soil and sediment.

关键词： black carbon humic acid phenanthrene sorption attenuation

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Gut bacterium promotes host fitness in special ecological niche by affecting sugar metabolism in Drosophila suzukii

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Insect Science 2023年第6期30卷 1713-1733页

作者： Huan-Huan Gao Shan Zhao Rui-Juan Wang Dong-Yun Qin Peng Chen An-Sheng Zhang Qian-Ying Zhuang Yi-Fan Zhai Xian-Hong Zhou Institute of plant protection Shandong Academy of Agricultural SciencesJinanChina shandong Academy of Grape JinanChina

As an important fruit pest of global significance,Drosophila suzukii occupies a special ecological niche,with the characteristics of high sugar and low protein *** niche differs from those occupied by other fruit-damaging Drosophila *** bacteria substantially impact the physiology and ecology of ***,the contribution of gut microbes to the fitness of *** in their special ecological niche remains *** this study,the effect of Klebsiella oxytoca on the development of *** was examined at physiological and molecular *** results showed that,after the removal of gut microbiota,the survival rate and longevity of axenic *** decreased *** of *** to the midgut of *** advanced the development level of *** differentially expressed genes and metabolites between axenic and ***-reintroduced *** were enriched in the pathways of carbohydrate *** advancement was achieved through an increased glycolysis rate and the regulation of the transcript level of key genes in the glycolysis/gluconeogenesis *** oxytoca is likely to play an important role in increasing host fitness in their high-sugar ecological niche by stimulating the glycolysis/gluconeogenesis *** a protein source,bacteria can also provide direct nutrition for ***,which depends on the quantity or biomass of *** result may provide a new target for controlling *** by inhibiting sugar metabolism through eliminating the effect of *** and thus disrupting the balance of gut microbial communities.

关键词： carbohydrate metabolism Drosophila suzuki glycolysis/gluconeogenesis Klebsiella oxytoca symbiotic bacteria

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Hydrobios and Control of Eutrophication in Dongping Lake,shandong Province

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Acta Geologica Sinica(English Edition) 2000年第2期74卷 329-333页

作者： DOU Suzhen SUN Zhaocai CAO Ying MA Yumei ZHANG Jingfu LIU Guicheng KOU Xiaoyan Environmental protection and Monitoring station of Taian City Tai’an 271000 Shandong Environmental protection Bureau of Dongping County Dongping 271500 Shandong

The Hydrobios in Dongping Lake, shandong Province, mainly includes phytoplankton, zooplankton, benthic animals, fishes and hydrophytic vascular plants. Nitrogen and phosphorus are the restrictive factors for the growth and propagation of hydrophyta, therefore the key to the prevention and control of eutrophication in lakes lies in the control of the contents of the two elements in the water. Artificial fishing of algae can reduce the concentrations of trophic substances such as nitrogen and phosphorus in the water bodies, and biological measures may decrease the contents of these trophic substances in the bottom sediments and the water bodies, thereby playing an active role in modifying the eutrophication of the lake.

关键词： Dongping Lake hydrobios eutrophication biological evaluation prevention and control measures

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Molecular identification and enzymatic properties of laccase2 from the diamondback moth Plutella xylostella (Lepidoptera:Plutellidae)

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Journal of Integrative Agriculture 2018年第10期17卷 2310-2319页

作者： LIU Zhen-gang WANG Huan-huan XUE Chao-bin Key Laboratory of Pesticide Toxicology and Application Technique College of Plant Protection Shandong Agricultural University

Laccase（EC 1.***.3.2）is known to oxidize various aromatic and nonaromatic compounds via a radical-catalyzed reaction,which generally includes two types of laccase,Lac1 and ***1 oxidizes toxic compounds in the diet,and Lac2 is known to play an important role in melanizing the insect *** this study,we cloned and sequenced the cDNA of the diamondback moth,Plutella xylostella Lac2（PxLac2）,from the third instar larvae using polymerase chain reaction（PCR）and rapid amplification of cDNA ends *** results showed that the full-length PxLac2 cDNA was 1 944 bp long and had an open reading frame of 1 794 ***2 encoded a protein with 597 amino acids and had a molecular weight of 66.09 ***,we determined the expression levels of PxLac2 in different stages by quantitative PCR（qPCR）.The results indicated that PxLac2 was expressed differently in different *** observed the highest expression level in pupae and the lowest expression level in fourth instar *** also investigated the enzymatic properties of laccase,which had optimal activity at pH 3.0 and at 35°*** these optimal conditions,laccase had a Michaelis constant（Km）of 0.97 mmol L-1,maximal reaction speed（Vm）of 56.82 U mL-1,and activation energy（Ea）of 17.36 kJ mol-1 to oxidize2,2＇-azino-bis（3-ethylbenzothiazoline-6-sulfonic acid ammonium salt）.Type II copper enhanced laccase activity below 0.8mmol L-1 and reduced enzyme activity above 0.8 mmol L-1 with an IC50 concentration of 1.26 mmol *** study provides insights into the biological function of laccase.

关键词： laccase melanization in vivo expression enzymatic properties Plutella xylostella

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