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Identification prognostic features related to sphingolipid metabolism and experimental validation of TRIM47 in hepatocellular carcinoma

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BIOCELL 2024年第4期48卷 639-651页

作者： JIAN TANG CHENQIANG ZHU YUN CHEN YUNLONG WU MING HE YI ZHOU MINGHUA XIE Department of Intensive Care Unit The First People’s Hospital of Linping DistrictHangzhouChina Department of Pharmacy The First People’s Hospital of Linping DistrictHangzhouChina

Background:The speciﬁc impact of sphingolipid metabolism on developing hepatocellular Carcinoma(HCC)remains *** study aims to explore the relationship between sphingolipid metabolism and HCC prognosis,immune response,and drug ***:Data were obtained from The Cancer Genome Atlas(TCGA)-Hepatocellular Carcinoma(LIHC)and Gene Expression Omnibus(GEO,GSE14520 datasets).47 sphingolipid metabolism genes were obtained from the Kyoto Encyclopedia of Genes and Genomes(KEGG)*** classifying HCC samples using the Non-negative Matrix Factorization(NMF)clustering method,differentially expressed genes were ***,8 risk genes were obtained by univariate analysis,survival random forest reduction and lasso *** expression of 8 risk genes was veriﬁed in ***:8 risk genes were used to construct the Sphingolipid score ***-Sphingolipid score predicted poor prognosis of HCC *** score was associated with immune checkpoints(IL-1B,TLR4,TGFB1,and IL-10),immune cells(Th2,Treg,MDSC,Neutrophil,Fibroblasts and macrophage),and MAPK *** the High-Sphingolipid score group,a signiﬁcantly higher proportion of patients with TP53(p53)mutations was signiﬁcantly higher(56%).Furthermore,patients with a high-Sphingolipid score were predicted to have a higher sensitivity to chemotherapy *** vitro validation showed that compared with normal liver cells LX-2,TRIM47,and S100A9 signiﬁcantly increased in liver cancer cells Hep G2,MHCC-97H,and Hep3B2.1-7,while SLC1A7,LPCAT1,and CFHR4 signiﬁcantly *** TRIM47 reduced the proliferation and promoted *** levels of ceramide synthesis-related indexes(CERS1,CERS6,CERS5,and SPTLC2)increased,and the ACER3 related to catalytic hydrolysis ***:We constructed a sphingolipid metabolism-related prognostic signature(Sphingolipid score)based on 8 risk ***47 may affect the development of liver cancer by regulating the relevant indicators of

关键词： Hepatocellular carcinoma Sphingolipid metabolism TRIM47 Prognosis

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Safflower Yellow Inhibits Progression of Hepatocellular Carcinoma by Modulating Immunological Tolerance via FAK Pathway

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Chinese Journal of Integrative Medicine 2024年第4期30卷 339-347页

作者： JI Hua-feng YANG Zi-qiang HAN Jun-jun LI He-fang JIN Zhao-qing CHEN Wei-qing CHEN Fei-hua GONG Mou-chun Department of General Surgery First People's Hospital of Hangzhou Lin'an DistrictHangzhou(311300)China

Objective To explore the anti-tumor effect of safflower yellow(SY)against hepatocellular carcinoma(HCC)and the underlying potential *** An in vitro model was established by mixing Luc-Hepa1-6 cells and CD3^(+)CD8^(+)T cells,followed by adding programmed cell death protein 1(PD-1)antibody(Anti-mPD-1)with or without *** apoptosis was detected by flow cytometry and the level of inflammatory cytokines was determined by enzyme-linked immunosorbent *** protein levels of programmed cell death 1 ligand 1(PD-L1),chemokine ligand(CCL5),C-X-C motif chemokine ligand 10(CXCL10)were measured by Western *** in situ animal model was established in mice followed by treatment with anti-mPD-1 with or without *** imaging was monitored with an AniView 100 imaging *** establish the FAK-overexpressed Luc-Hepa1-6 cells,cells were transfected with adenovirus containing pcDNA3.1-FAK for 48 *** The fluorescence intensity,apoptotic rate,release of inflammatory cytokines,and CCL5/CXCL10 secretion were dramatically facilitated by anti-mPD-1(P<0.01),accompanied by an inactivation of PD-1/PD-L1 axis,which were extremely further enhanced by SY(P<0.05 or P<0.01).Increased fluorescence intensity,elevated percentage of CD3+CD8+T cells,facilitated release of inflammatory cytokines,inactivated PD-1/PD-L1 axis,and increased CCL5/CXCL10 secretion were observed in Anti-mPD-1 treated mice(P<0.01),which were markedly enhanced by SY(P<0.05 or P<0.01).Furthermore,the enhanced effects of SY on inhibiting tumor cell growth,facilitating apoptosis and inflammatory cytokine releasing,suppressing the PD-1/PD-L1 axis,and inducing the CCL5/CXCL10 secretion in Anti-mPD-1 treated mixture of Luc-Hepa1-6 cells and CD3+CD8+T cells were abolished by FAK overexpression(P<0.01).Conclusion SY inhibited the progression of HCC by mediating immunological tolerance through inhibiting FAK.

关键词： hepatocellular carcinoma safflower yellow Chinese medicine tumor microenvironment programmed cell death protein 1 focal adhesion kinase

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Efficacy and safety of minimally invasive laparoscopic surgery under general anesthesia for ovarian cancer

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World Journal of Clinical Cases 2024年第9期12卷 1569-1577页

作者： Xian Qin Chen Chen Yang Liu Xian-Hong Hua Jia-Yi Li Meng-Jie Liang Fang Wu Department of Anesthesiology The First People's Hospital of Jiangxia DistrictWuhan 430200Hubei ProvinceChina Department of Obstetrics and Gynecology The First People's Hospital of Jiangxia DistrictWuhan 430200Hubei ProvinceChina

BACKGROUND Ovarian cancer is one of the most common malignant tumors in female reproductive system in the world,and the choice of its treatment is very important for the survival rate and prognosis of *** open surgery is the main treatment for ovarian cancer,but it has the disadvantages of big trauma and slow *** the continuous development of minimally invasive technology,minimally invasive laparoscopic surgery under general anesthesia has been gradually applied to the treatment of ovarian cancer because of its advantages of less trauma and quick ***,the efficacy and safety of minimally invasive laparoscopic surgery under general anesthesia in the treatment of ovarian cancer are still *** To explore the efficacy and safety of general anesthesia minimally invasive surgery in the treatment of ovarian *** The clinical data of 90 patients with early ovarian cancer in our hospital were analyzed *** to the different surgical treatment methods,patients were divided into study group and control group(45 cases in each group).The study group received minimally invasive laparoscopic surgery under general anesthesia for ovarian cancer,while the control group received traditional open surgery for ovarian *** European Organization for Research and Treatment of Cancer Quality of Life Questionnaire(EORTC QLQ-C30),clinical efficacy and safety of the two groups were *** The intraoperative blood loss,length of hospital stay,postoperative gas evacuation time,and postoperative EORTC QLQ-C30 score of the study group were significantly better than those of the control group(P<0.05).The incidence of postoperative complications in the study group was significantly lower than in the control group(P<0.05).The two groups had no significant differences in the preoperative adrenocorticotropic hormone(ACTH),androstenedione(AD),cortisol(Cor),cluster of differentiation 3 positive(CD3+),and clu

关键词： Early-stage ovarian cancer Efficacy Minimally invasive Laparoscopy Safety Surgery

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Effects of vitrectomy combined with internal limiting membrane peeling in patients with diabetic macular edema

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World Journal of Clinical Cases 2024年第21期12卷 4491-4498页

作者： Lei Wang Chun-Jie Chen Ming-Li Wang Yong Huang Li-Jian Fang Department of Ophthalmology Liangxiang Hospital of Beijing Fangshan DistrictBeijing 102400China

BACKGROUND Diabetic macular edema(DME),a chronic microvascular complication of diabetes,is a leading cause of visual impairment and *** plana vitrectomy(PPV)can restore the normal macular structure and reduce macular edema,whereas internal limiting membrane(ILM)peeling is used to treat tractional macular *** the advantages,there is limited research on the combined effects of PPV with ILM *** To observe the effects of PPV combined with ILM peeling on postoperative central macular thickness(CMT),best-corrected visual acuity(BCVA),cystoid macular edema(CME)volume,and complications in patients with *** Eighty-one patients(92 eyes)diagnosed with DME at the Beijing Shanqu Liangxiang hospital between January and December 2022 were randomly divided to undergo PPV alone(control group:41 patients,47 eyes)or PPV+ILM peeling(stripping group:40 patients,45 eyes);a single surgeon performed all *** two groups were compared preoperatively and 1 and 3 months *** Preoperatively,both groups had comparable values of CMT,BCVA,and CME volume(P>0.05).After surgery(both 1 and 3 months),both groups showed significant reductions in CMT,BCVA,and CME volume compared to preoperative levels,with the stripping group showing more significant reductions compared to the control group(P<0.05).Further repeated-measures ANOVA analysis for within-group differences revealed significant effects of group and time,and interaction effects for CMT,BCVA,and CME volume(P<0.05).There were no significant differences in the incidence of complications between the groups(retinal detachment:control=2,stripping=1;endophthalmitis:Control=4,stripping=1;no cases of secondary glaucoma or macular holes;χ^(2)=0.296,P=0.587).CONCLUSION PPV with ILM peeling can significantly improve the visual acuity of patients with DME,reduce CMT,and improve CME with fewer complications.

关键词： Vitrectomy Internal limiting membrane peeling Diabetic macular edema Central macular thickness Bestcorrected visual acuity Complications

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Psychological intervention based on social cognitive theory: Treating pain, anxiety, and depression in perioperative patients

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World Journal of Psychiatry 2024年第8期14卷 1199-1207页

作者： Hai-Jian Mao Lin-Fei Wang Chun Lin Department of Surgical Anesthesiology The First People’s Hospital of Lin’an DistrictHangzhouHangzhou 311300Zhejiang ProvinceChina Department of Gastrointestinal Surgery Hangzhou First People’s HospitalHangzhou 310006Zhejiang ProvinceChina Department of Comprehensive Intervention The First People’s Hospital of Lin’an DistrictHangzhouHangzhou 311300Zhejiang ProvinceChina

BACKGROUND Surgery is an effective method for treating certain *** such as disease,preoperative fear and tension,surgical stress,postoperative pain,and related complications directly affect the smooth progression and outcome of *** may experience a series of psychological and physiological changes during the perioperative period,resulting in anxiety and depression,which may reduce the pain threshold and worsen their *** We enrolled 200 patients who underwent surgical care at The first People’s hospital of Lin’an district,Hangzhou between January and December *** were categorized into a routine intervention group(n=103)and a psychological intervention group(n=97),based on the intervention strategies *** assessment tools,including the self-rating anxiety scale(SAS),the self-rating depression scale(SDS),and the Connor–Davidson Resilience scale,were used to measure patients’negative states and *** pre-and post-intervention scores for these metrics in the two groups were then *** In the psychological intervention group,the SAS and SDS scores(31.56±5.18 and 31.46±4.57,respectively)were significantly reduced compared to the routine intervention group(P<0.05).The visual analog scale pain scores at 12 and 24 hours after intervention(6.85±1.21,4.24±0.72)were notably higher than those in the routine intervention group(P<0.05).The psychological intervention group also demonstrated superior scores in perseverance(36.08±3.29),self-reliance(22.63±2.91),optimism(11.42±1.98),and resilience(70.13±5.37),compared to the routine intervention group(P<0.05).Additionally,the psychological intervention group’s confrontation score(23.16±4.29)was higher(P<0.05).This group also reported lower scores in avoidance(9.28±1.94)and yielding(6.19±1.92)(P<0.05).Lastly,the Short Form 36 Health Survey scores were significantly higher in the psychological intervention group,indicating a better quality of life(P<0.05).CONCLUSION

关键词： Perioperative period Social cognitive theory Psychological intervention Pain Anxiety and depression

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Effect of nursing on postoperative respiratory function and mental health of lung cancer patients

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World Journal of Clinical Cases 2024年第5期12卷 922-930页

作者： Xiang Yang Dan Yin Shi-Qing Chen Department of Rehabilitation The First People's Hospital of Jiangxia DistrictWuhan(Union Jiangnan Hospital Huazhong University of Science and Technology)Wuhan 430200Hubei ProvinceChina Department of Intensive Care The First People's Hospital of Jiangxia DistrictWuhan(Union Jiangnan Hospital Huazhong University of Science and Technology)Wuhan 430200Hubei ProvinceChina Department of Neurology The First People's Hospital of Jiangxia DistrictWuhan(Union Jiangnan Hospital Huazhong University of Science and Technology)Wuhan 430200Hubei ProvinceChina

BACKGROUND Both pulmonary rehabilitation training and psychological care have been shown to have a positive effect on the postoperative recovery of patients with lung ***,few studies have combined the two to explore their combined ***,this study aimed to investigate the effects of pulmonary rehabil-itation training combined with psychological care on postoperative respiratory function and mental health in lung cancer *** To investigate effect of nursing on postoperative respiratory function and mental health of lung cancer *** 122 cases of lung cancer patients who underwent surgical treatment in our hospital and were treated in our department from January 2022 to April 2023 were selected and randomly divided into the control group and observation *** control group performed the routine care *** obser-vation group was given pulmonary rehabilitation training and psychological care based on conventional nursing *** expiratory volume,forced vital *** ventilatory volume(MVV)in one second was measured,and the patient's 6-min walking distance and dyspnoea index scale were used to assess the patient's respiratory *** Connor-Davidson resilience scale(CD-RISC),self-rating anxiety scale(SAS),and self-rating depression scale(SDS)were used to evaluate the mental health of the *** There was no difference between the two groups regarding age,gender,education level,surgical procedure,type of pathology,and treatment(P>0.05).After treatment,MVV,6-min walking distance,toughness,strength,optimism,and total CD-RISC scores were significantly higher in the observation group(P<0.05),dyspnoea scores,SAS,and SDS scores were substantially lower in the control group compared to the observation group(P<0.05).CONCLUSION Pulmonary rehabilitation training combined with psychological care for patients after lung cancer resection could improve lung function,enhance daily activities,e

关键词： Pulmonary rehabilitation training Psychological care Lung cancer Postoperative care Respiratory function

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Recent advances to address challenges in extracellular vesicle-based applications for lung cancer

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Acta Pharmaceutica Sinica B 2024年第9期14卷 3855-3875页

作者： Gaigai Huang Wenshu Zheng Yu Zhou Meihua Wan Tony Hu Department of Clinical Laboratory the First People’s Hospital of Shuangliu District(West China Airport Hospital of Sichuan University)Chengdu 610200China Center for Cellular and Molecular Diagnostics Tulane University School of MedicineNew OrleansLA 70112USA Department of Integrated Traditional Chinese and Western Medicine West China Hospital of Sichuan UniversityChengdu 610200China The first People’s hospital of Shuangliu district(West China Airport hospital of Sichuan University) Chengdu 610200China

Lung cancer,highly prevalent and the leading cause of cancer-related death globally,persists as a significant challenge due to the lack of definitive tumor markers for early diagnosis and personalized therapeutic ***,extracellular vesicles(EVs),functioning as natural carriers for intercellular communication,have received increasing attention due to their ability to traverse biological barriers and deliver diverse biological cargoes,including cytosolic proteins,cell surface proteins,microRNA,lncRNA,circRNA,DNA,and *** are increasingly recognized as a valuable resource for non-invasive liquid biopsy,as well as drug delivery platforms,and anticancer vaccines for precision medicine in lung ***,given the diagnostic and therapeutic potential of tumor-associated EVs for lung cancer,we discuss this topic from a translational *** delve into the specific roles that EVs play in lung cancer carcinogenesis and offer a particular perspective on how advanced engineering technologies can overcome the current challenges and expedite and/or enhance the translation of EVs from laboratory research to clinical settings.

关键词： Lung cancer Extracellular vesicles Therapy resistance Delivery Diagnostics Therapeutics Nanotechnology Engineering

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Neferine inhibits the progression of diabetic nephropathy by modulating the miR-17-5p/nuclear factor E2-related factor 2 axis

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Journal of Traditional Chinese Medicine 2024年第1期44卷 44-53页

作者： HUANG Hongmei YANG Maojun LI Ting WANG Dandan LI Ying TANG Xiaochi YUAN Lu GU Shi XU Yong Department of Endocrinology Chengdu Shuangliu District First People's Hospital(West China Airport of Sichuan University)Chengdu 610200China Department of Endocrinology Affiliated Hospital of Southwest Medical UniversityLuzhou 646000China

OBJECTIVE:To investigate the effect of Neferine(Nef)on diabetic nephropathy(DN)and to explore the mechanism of Nef in DN based on miRNA regulation ***:A DN mouse model was constructed and treated with *** creatinine(Crea),blood urea(UREA)and urinary albumin were measured in mice by kits,and renal histopathological changes and fibrosis were observed by hematoxylin-eosin staining and Masson *** tissue superoxide dismutase(SOD),malondialdehyde(MDA)and glutathione peroxidase(GSH-Px)activities were measured by enzyme-linked immunosorbent assay(ELISA).Western blotting was used to detect the expression of nuclear factor E2-related factor 2(Nrf2)/heme oxygenase 1(HO-1)signaling pathway-related proteins in kidney *** reverse transcription-polymerase chain reaction(q RT-PCR)was used to detect the expression of miR-17-5p in kidney ***,a DN in vitro model was constructed by high glucose culture of human mesangial cells(HMCs),cells were transfected with miR-17-5p mimic and/or treated with Nef,and we used q RTPCR to detect cellular miR-17 expression,flow cytometry to detect apoptosis,ELISAs to detect cellular SOD,MDA,and GSH-Px activities,Western blots to detect Nrf2/HO-1 signaling pathway-related protein expression,and dual luciferase reporter gene assays to verify the targeting relationship between Nrf2 and ***:Administration of Nef significantly reduced the levels of blood glucose,Crea,and UREA and the expression of miR-17-5p,improved renal histopathology and fibrosis,significantly reduced MDA levels,elevated SOD and GSH-Px activities,and activated Nrf2 expression in kidney tissues from mice with ***2 is a post-transcriptional target of *** HMCs transfected with miR-17-5p mimics,the m RNA and protein levels of Nrf2 were significantly ***,miR-17-5p overexpression and Nef intervention resulted in a significant increase in high glucose-induced apoptosis and MDA levels in HMC

关键词： diabetic nephropathies neferine miR-17-5p NF-E2-related factor 2 oxidative stress

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Impact of nutritional support on immunity,nutrition,inflammation,and outcomes in elderly gastric cancer patients after surgery

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World Journal of Gastrointestinal Surgery 2024年第7期16卷 2175-2182页

作者： Xiao-Wan Chen Xiao-Chun Guo Fen Cheng Department of Gastrointestinal Surgery Hubei Cancer HospitalWuhan 430079Hubei ProvinceChina Department of Gastroenterology The First People’s Hospital of Jiangxia DistrictWuhan 430200Hubei ProvinceChina

BACKGROUND Postoperative rehabilitation of elderly patients with gastric cancer has always been the focus of clinical *** the intervention by a full-course nutritional support team can have a positive impact on the postoperative immune function,nutritional status,inflammatory response,and clinical outcomes of this special population has not yet been fully *** To evaluate the impact of full-course nutritional support on postoperative comprehensive symptoms in elderly patients with gastric *** This is a retrospective study,including 60 elderly gastric cancer patients aged 70 years and above,divided into a nutritional support group and a control *** nutritional support group received full postoperative nutritional support,including individualized meal formulation,and intravenous and parenteral nutrition supplementation,and was regularly evaluated and adjusted by a professional nutrition *** control group received routine postoperative *** After intervention,the proportion of CD4+lymphocytes(25.3%±3.1%vs 21.8%±2.9%,Phospital stay(10.3 d±2.1 d vs 14.8 d±3.6 d,P<0.05)and lower incidence of infection(15%vs 35%,P<0.05)in those of the control *** The intervention by the nutritional support team has a positive impact on postoperative immune function,

关键词： Elderly gastric cancer Postoperative Nutritional support Immune function Inflammatory response Clinical outcome

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Ghrelin inhibits autophagy mediated by AKT/mTOR pathway to ameliorate retinal angiogenesis induced by high glucose stress

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International Journal of Ophthalmology(English edition) 2024年第5期17卷 785-793页

作者： Jiang-Li Fan Rong Li Min Zhang Chao Chen Guo-Min Yao Ling-Xiao Zhou Department of Ophthalmology Gongshu District People’s Hospital of Integrated Traditional Chinese and Western MedicineHangzhou 310004Zhejiang ProvinceChina Department of Ophthalmology the First Affiliated Hospital of Xi’an Medical UniversityXi’an 710077Shaanxi ProvinceChina Department of Endocrinology the First Affiliated Hospital of Xi’an Medical UniversityXi’an 710077Shaanxi ProvinceChina Department of General Surgery the First Affiliated Hospital of Xi’an Medical UniversityXi’an 710077Shaanxi ProvinceChina

AIM:To observe the effect of ghrelin,a growth hormonereleasing peptide,on retinal angiogenesis in vitro under high glucose(HG)stress and to explore the possible mechanism of ***:Human retinal microvascular endothelial cells(HRMECs)were treated with high concentration of glucose alone or in combination with *** cell migration,tube formation and the expression of the autophagy-related proteins LC3-II/I,Beclin-1,p62,phosphorylated AKT(p-AKT)/AKT and phosphorylated mammalian target of rapamycin(p-mTOR)/mTOR were ***,to clarify the correlation between ghrelin effect and autophagy,AKT inhibitor VIII was adopted to treat HRMECs,and cell migration,tube formation as well as the protein expressions of LC3-II/I,Beclin-1 and p62 were ***:Under HG stress,ghrelin inhibited migration and tube formation of *** inhibited the increases in the protein levels of LC3-II/I,Beclin-1 and the decreases in the protein levels of p62,p-AKT/AKT and p-mTOR/mTOR induced by HG ***,under the action of AKT/mTOR pathway inhibitors,the effects of ghrelin on migration and tube formation were both *** addition,the expression of LC3-II/I and Beclin-1 were significantly up-regulated and the expression of p62 was ***:Retinal angiogenesis under in vitro HG stress can be inhibited by ghrelin through activating AKT/mTOR pathway to inhibit autophagy.

关键词： ghrelin retinal endothelial cell angiogenesis autophagy stress

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