检索结果-南通市图书馆

AstragalosideⅣplays a role in reducing radiation-induced liver inflammation in mice by inhibiting thioredoxin-interacting protein/nod-like receptor protein 3 signaling pathway

引用

Journal of Traditional Chinese Medicine 2023年第1期43卷 87-94页

作者： DING Yanping DONG Xiaoqing MA Yifan CHEN Lili ZHOU Jie LI Xinyan SHAO Baoping college of life science Northwest Normal UniversityLanzhou 730000China college of life science Lanzhou UniversityLanzhou 730000China

OBJECTIVE:To investigate the efficacy of Astragaloside IV(AS-IV)on radiation-induced liver inflammation in mice.METHODS:The mice were divided into normal group,dimethyl sulfoxide solvent group,irradiation group(IR),irradiation+AS-IV(20 mg/kg)group(IR+AS-20)and irradiation+AS-IV(40 mg/kg)group(IR+AS-40).One month after intraperitoneal injection of AS-IV,the mice were irradiated with 8Gry Co60γ,the blood was collected for biochemical analysis,and the liver was collected for hematoxylin-eosin staining,immunofluorescence and electron microscopic observation,oxidative stress,and Western blot analysis.RESULTS:The AS-IV treatment significantly ameliorated the pathological morphology of liver and reduced the alanine aminotransferase and aspertate aminotransferase levels in serum induced by radiation;AS-IV treatment also significantly reduced the expression of inflammatory factors tumor necrosis factor alpha and interleukin 6 and antagonized malonaldehyde content and superoxide dismutase activity in liver caused by radiation;in addition,AS-IV treatment can significantly inhibited the positive expression of thioredoxin-interacting protein(TXNIP)and nod-like receptor protein 3(NLRP3)inflammasome in liver tissue after radiation;The expression of TXNIP,NLRP3 inflammasome,apoptosisassociated speck-like protein containing a CARD,cysteinyl aspartate-specific proteinase 1 and interleukin 1beta in the AS-IV prevention group decreased significantly compared to the radiation group.CONCLUSIONS:These findings suggested that Co60γradiation can cause structural and functional damage to the liver,which may be related to the NLRP3 mediated inflammatory pathway;AS-IV may play a protective role by inhibiting the TXNIP/NLRP3 inflammasome signaling pathway in the radiation-induced liver injury model.

关键词： radiation,ionizing astragalosideⅣ liver inflammation NLR family,pyrin domain-containing 3 protein thioredoxin-interacting protein

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Selective immobilization of Pb(Ⅱ)by biogenic whewellite and its mechanism

引用

Journal of Environmental sciences 2024年第3期137卷 664-676页

作者： Xingxing Wang Kaiyan Ren Kairui Jiao Wenjun Nie Xiaochi An Bin Lian college of life sciences College of Marine Science and EngineeringNanjing Normal UniversityNanjing 210023China

The development of bio-adsorbents with highly selective immobilization properties for specific heavy metals is a great challenge,but has important application value.Biogenic whewellite(BW)with high selectivity for Pb(Ⅱ)was synthesized by mineral microbial transformation.The selective immobilization properties and mechanism of BW for Pb(Ⅱ)were analyzed by combining mineral characterization technology and batch adsorption research methods.The results indicated that BW can efficiently and selectively immobilize Pb(Ⅱ)in single or composite heavy metal adsorption solutions,and the immobilized Pb(Ⅱ)is difficult to desorb.BW undergoes monolayer adsorption on Pb(Ⅱ),Qmax≈1073.17 mg/g.The immobilization of Pb(Ⅱ)by BW is a physico-chemical adsorption process with spontaneous heat absorption and an accompanying increase in entropy.In addition,the sequestration of Pb(Ⅱ)by BW remains around 756.99 mg/g even at p H=1.The excellent selective immobilization properties of BW for Pb(Ⅱ)are closely related to its smaller Ksp,electrostatic repulsion effect,organic-inorganic composite structure,acid resistance and the formation of Pb(Ⅱ)oxalate.This study provides beneficial information about the recycling of lead in acidic lead-containing wastewater and composite heavy metal contaminated water bodies.

关键词： Aspergillus niger Biogenic whewellite Pb(Ⅱ) Selective adsorption Immobilization mechanism

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Proteomic characterization of four subtypes of M2 macrophages derived from human THP-1 cells

引用

Journal of Zhejiang University-science B(Biomedicine & Biotechnology) 2022年第5期23卷 407-422页

作者： Pengfei LI Chen MA Jing LI Shanshan YOU Liuyi DANG Jingyu WU Zhifang HAO Jun LI Yuan ZHI Lin CHEN Shisheng SUN college of life science Northwest UniversityXi'an 710069China

Macrophages are widely distributed immune cells that contribute to tissue homeostasis.Human THP-1 cells have been widely used in various macrophage-associated studies,especially those involving pro-inflammatory M1 and anti-inflammatory M2 phenotypes.However,the molecular characterization of four M2 subtypes(M2a,M2b,M2c,and M2d)derived from THP-1has not been fully investigated.In this study,we systematically analyzed the protein expression profiles of human THP-1-derived macrophages(M0,M1,M2a,M2b,M2c,and M2d)using quantitative proteomics approaches.The commonly and specially regulated proteins of the four M2 subtypes and their potential biological functions were further investigated.The results showed that M2a and M2b,and M2c and M2d have very similar protein expression profiles.These data could serve as an important resource for studies of macrophages using THP-1 cells,and provide a reference to distinguish different M2 subtypes in macrophage-associated diseases for subsequent clinical research.

关键词： Macrophage THP-1 cells M2 subtype Proteomics Biological function

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

TRAF7 negatively regulates the RLR signaling pathway by facilitating the K48-linked ubiquitination of TBK1

引用

Virologica Sinica 2023年第3期38卷 419-428页

作者： Jing-Ping Huang Ya-Xian Yang Tian Chen Dan-Dan Wang Jing Li Liang-Guo Xu college of life science Jiangxi Normal UniversityNanchang330022China

TANK-binding kinase 1(TBK1)is a nodal protein involved in multiple signal transduction pathways.In RNA virus-mediated innate immunity,TBK1 is recruited to the prion-like platform formed by MAVS and subsequently activates the transcription factors IRF3/7 and NF-κB to produce type I interferon(IFN)and proinflammatory cytokines for the signaling cascade.In this study,TRAF7 was identified as a negative regulator of innate immune signaling.TRAF7 interacts with TBK1 and promotes K48-linked polyubiquitination and degradation of TBK1 through its RING domain,impairing the activation of IRF3 and the production of IFN-β.In addition,we found that the conserved cysteine residues at position 131 of TRAF7 are necessary for its function toward TBK1.Knockout of TRAF7 could facilitate the activation of IRF3 and increase the transcript levels of downstream antiviral genes.These data suggest that TRAF7 negatively regulates innate antiviral immunity by promoting the K48-linked ubiquitination of TBK1.

关键词： TANK-Binding kinase 1(TBK1) Type I interferon TRAF7 Ubiquitination Innate immunity

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Structural characterization of four Rhododendron spp.chloroplast genomes and comparative analyses with other azaleas

引用

BIOCELL 2023年第3期47卷 657-668页

作者： XIAOJUN ZHOU MENGXUE LIU LINLIN SONG life science college Luoyang Normal UniversityLuoyang471934China college of life science and Technology Henan Institute of Science and TechnologyXinxiang453003China

Azalea is a general designation of Rhododendron in the Ericaceae family.Rhododendron not only has high ornamental value but also has application value in ecological protection,medicine,and scientific research.In this study,we used Illumina and PacBio sequencing to assemble and annotate the entire chloroplast genomes(cp genomes)of four Rhododendron species.The chloroplast genomes of R.concinnum,R.henanense subsp.lingbaoense,R.micranthum,and R.simsii were assembled into 207,236,208,015,207,233,and 206,912 bp,respectively.All chloroplast genomes contain eight rRNA genes,with either 88 or 89 protein-coding genes.The four cp genomes were compared and analyzed by bioinformatics,and the phylogenetic analysis based on chloroplast genomes of 26 species of Ericaceae,Actinidiaceae,and Primulaceae under Ericales was conducted.A comparison of the linear structure of cp genomes of four Rhododendron showed that there were substantial sequence similarities in coding regions,but high differences in non-coding regions.A phylogenetic analysis,based on chloroplast whole genome sequences,showed that all Rhododendron species are in the clade Ericaceae.This study provides valuable genetic information for the study of population genetics and evolutionary relationships in Rhododendron and other azalea species.

关键词： Rhododendron Comparative analysis Repeat sequences Phylogeny

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Bavachin induces apoptosis in colorectal cancer cells through Gadd45a via the MAPK signaling pathway

引用

Chinese Journal of Natural Medicines 2023年第1期21卷 36-46页

作者： WANG Mengru TIAN Baopeng SHEN Jie XU Shilin LIU Cong GUAN Ling GUO Min DOU Jie college of life science and Technology China Pharmaceutical UniversityNanjing 211198China

Bavachin is a dihydroflavonoid compound isolated from Psoralea corylifolia,and exhibits anti-bacterial,anti-inflammatory,anti-tumor and lipid-lowering activities.Recent attention has gradually drawn on bavachin-induced apoptosis in many human cancer cell lines.However,the anti-cancer effects and related mechanisms in colorectal cancer remain unknown.Here,we investigated the effects of bavachin on colorectal cancer in vivo and in vitro.The results showed that bavachin inhibited the proliferation of human colorectal cancer cells and induce apoptosis.These changes were mediated by activating the MAPK signaling pathway,which significantly up-regulated the expression of Gadd45a.Furthermore,Gadd45a silencing obviously attenuated bavachin-mediated cell apoptosis.Inhibition of the MAPK signaling pathway by JNK/ERK/p38 inhibitors also weakened the up-regulation of Gadd45a by bavachin.The anticancer effect of bavachin was also validated using a mouse xenograft model of human colorectal cancer.In conclusion,these findings suggest that bavachin induces the apoptosis of colorectal cancer cells through activating the MAPK signaling pathway.

关键词： Bavachin Apoptosis Colorectal cancer Gadd45a MAPK

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effects of 5-Azacytidine(AZA)on the Growth,Antioxidant Activities and Germination of Pellicle Cysts of Scrippsiella acuminata(Dinophyceae)

引用

Journal of Ocean University of China 2023年第6期22卷 1660-1668页

作者： WANG Zhaohui ZHANG Jianneng TANG Tao ZHANG Yuning HU Ren college of life science and Technology Jinan UniversityGuangzhou 510632China

In this study,we investigated the effects of different concentrations of 5-azacytidine(AZA),a DNA methyltransferase in-hibitor,on the growth,antioxidant activities and germination of pellicle cysts of Scrippsiella acuminata.The purpose of this study is to understand the toxic effects of AZA on marine microalgae,and to demonstrate the effect of DNA methyltransferase inhibitors on the germination of pellicle cysts.Results showed that AZA inhibited the growth of S.acuminata significantly,and displaced a clear dose-dependent inhibition trend with the 96h EC50 of 146.77μmolL^(-1)(35.84mgL^(-1)).Pellicle cysts of S.acuminata were less sensitive to AZA than the vegetative cells,and the EC50 value of AZA to the germination of pellicle cysts of S.acuminata was 8.08mmolL^(-1)(1.97g L^(-1)).After exposed to AZA,the antioxidant activities in S.acuminata responded rapidly and significantly.Among them,soluble pro-tein and superoxide dismutase(SOD)were more sensitive to AZA,and significant promotions occurred after exposed to 10μmolL^(-1)AZA for 24h.Meanwhile,malondialdehyde(MDA)contents in algal cells did not change significantly after exposed to low concen-trations of AZA,but increased firstly and then decreased under high concentration of AZA.The glutathione(GSH)levels in S.acu-minata increased significantly under high concentrations of AZA,and remained unchanged at low concentrations of AZA.The results suggested that the enhanced protein level and SOD activity of S.acuminata eliminated reactive oxygen species(ROS)to a certain ex-tent,and thus protected algal cells against damages of ROS caused by AZA.

关键词： 5-azacytidine Scrippsiella acuminata pellicle cysts toxicity superoxide dismutase glutathione malondialdehyde

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The fecal shield is a double-edged sword for larvae of a leaf beetle

引用

Current Zoology 2023年第2期69卷 173-180页

作者： Zheng-Zhong Huang Ze-Qun Dong Zu-Long Liang Bin Zhang Huai-Jun Xue Si-Qin Ge Institute of Zoology Chinese Academy of SciencesBeijing 100101China college of life sciences The Key Laboratory of Zoological Systematics and Application of Hebei ProvinceHebei UniversityBaoding 071002China college of life science Institute of Life Science and Green DevelopmentHebei UniversityBaoding 071002China college of life sciences Nankai UniversityTianin 300071China

Larvae of some leaf beetles carry masses of feces covering parts or all of the body,which is called a"fecal shield'In general,the shield is thought to be a defense structure against natural enemies.However,some studies have suggested that defense effectiveness varies depending on the natural enemy.In this study,we used a fecal retention leaf beetle Ophrida xanthospilota(Coleoptera:Chrysomelidae),and 2 local generalist predators(an ant,Camponotus japonicus(Hymenoptera:Formicidae)and a stinkbug,Arma custos(Hemiptera:Pentatomidael)as a system to test the hypothesis that the fecal shield of O.xanthospilota plays different roles in predation behavior of different predators and can provide multiple chemical communication signals in predator-prey interactions.Prey bioassays showed that the fecal shield of O.xanthospilota larvae repelled the ant C.japonicus while attracting the stinkbug A.custos.The results also strongly demonstrated that hexane extracts of the fecal shield significantly repelled C.japonicus,while dichloromethane(DCM)extracts did not inhibit ant predation.Interestingly,DCM extracts attracted A.custos,but hexane extracts did not.Therefore,we suggest that the fecal shield is a double-edged sword for the larvae of O.xanthospilota.Our results also indicated that the risk-benefit tradeoff of an insect should be estimated at a community level involving multiple enemies(predators and parasites)and herbivores,rather than in a single prey-predator pair.

关键词： ant chemical cues kairomone predatory stinkbug predator-prey interactions

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

1-Methylcyclopropene retards pak choi(Brassica rapa subsp.chinensis)yellowing via BcNAC055-,BcMYB44-,and BcOBF1-mediated regulation of the key chlorophyll degrading gene BcNYC1 during storage at 20℃

引用

Food Quality and Safety 2023年第1期7卷 75-86页

作者： Liuli Song Junping Wang Haitao Yu Zhifang Yu college of Food science and Technology Nanjing Agricultural UniversityNanjingChina college of life science Anhui Normal UniversityWuhuChina

The objective of this study was to investigate the molecular regulatory mechanism of 1-methylcyclopropene(1-MCP)treatment on leaf yellowing of pak choi during storage at 20°C.In the present study,compared with the control and 10μL/L ethylene-treated pak choi,5.0μL/L 1-MCP fumigation alleviated the yellowing process of pak choi as proved by the maintenance of higher levels of colour,chlorophyll content,and appearance.1-MCP treatment decreased chlorophyll breakdown by downregulating the activity of chlorophyll-degrading enzymes(chlorophyll b reductase(CBR)and pheophytinase(PPH))and the expression of their related genes(BcNYC1 and BcPPH).The application of 1-MCP also inhibited the transcript abundance of ethylene biosynthesis and signal transduction genes(BcACO,BcEIN3,and BcERF)and reduced ethylene production,thus helping to maintain the green colour of pak choi;however,ethylene treatment exerted the opposite function.Meanwhile,three transcription factors(BcNAC055,BcMYB44,and BcOBF1)were successfully cloned.Experiments performed using yeast one-hybrid assay,dual-luciferase reporter system and in vivo bioluminescence imaging assay,confirmed that BcNAC055,BcMYB44,and BcOBF1 directly bound to the BcNYC1 promoter.1-MCP inhibited the expression levels of BcNAC055,BcMYB44,BcOBF1,and BcNYC1 during storage,while the application of ethylene activated their expression.In conclusion,1-MCP downregulated the expression of the key chlorophyll degradation gene BcNYC1 by inhibiting the transcript level of its three positive regulators(BcNAC055,BcMYB44,and BcOBF1)and lowered the activity of chlorophyll-degrading enzyme CBR,thereby helping mitigate chlorophyll breakdown to retard leaf yellowing in pak choi.The results of this work extend our understanding of 1-MCP-delayed yellowing in postharvest pak choi.

关键词： Yllwing 1-methyIcyclopropene chlorophyIl degradation transcriptional regulation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Aptasensor Based on Screen-Printed Carbon Electrodes Modified with CS/AuNPs for Sensitive Detection of Okadaic Acid in Shellfish

引用

Journal of Analysis and Testing 2023年第2期7卷 128-135页

作者： Ni Zeng Xinyi Wang Yiyang Dong Yan Yang Yingai Yin Lianhui Zhao Xu Wang college of life science and Technology Beijing University of Chemical TechnologyBeijing 100029China

Okadaic acid(OA),a small molecule substance derived from shellfish,is one of the most widely distributed marine toxins with acute symptoms of vomiting and diarrhea after accidental ingestion.For this,there is an urgently need for sensitive and reliable methods to detect OA in real shellfish samples.In this study,a simple aptasensor based on screen-printed carbon electrode(SPCE)with modification of chitosan(CS)and gold nanoparticles(Au NPs)was designed for electrochemical determination of OA,and the electrode surface was modified with Au NPs by potential-sweeping electrodeposition,which greatly improved the electrochemical response.The entire detection and characterization process were carried out by cyclic voltammetry(CV)with a linear correlation in the range of 0.01-100 ng/m L and a limit of detection(LOD)of 6.7 pg/m L.Furthermore,recovery rates of 92.3-116%were obtained demonstrating excellent accuracy through the recovery trial of mussel and scallop samples.

关键词： Okadaic acid Aptasensor Screen-printed carbon electrode Chitosan Gold nanoparticles Cyclic voltammetry

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：