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Rapid detection of the rice false smut fungus Ustilaginoidea virens by lateral flow strip-based recombinase polymerase amplification assay

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Journal of Integrative Agriculture 2024年第11期 3763-3773页

作者： Jiacheng Xi Sanlian Wan Yue Li Yuandi Xu Jing Yang Ting Zhang Jiajia Chen Zhengguang Zhang Danyu Shen Haifeng Zhang Sanya Institute of Nanjing Agricultural University/Key Laboratory of Integrated Management of Crop Diseases and Pests of Ministry of Education Department of Plant Pathology College of Plant Protection Nanjing Agricultural University Sanya Extension Service Center for Agricultural Technology college of landscape architecture Jiangsu Vocational College of Agriculture and Forestry

Rice false smut, caused by Ustilaginoidea virens, is a devastating disease that greatly reduces rice yield and ***, controlling rice false smut disease is challenging due to the unique infection mode of U. virens. Therefore,there is a need for early diagnosis and monitoring techniques to prevent the spread of this disease. Lateral flow strip-based recombinase polymerase amplification(LF-RPA) overcomes the limitations of current U. virens detection technologies, which are time-consuming, require delicate equipment, and have a high false-positive rate. In this study, we used a comparative genomics approach to identify Uv＿3611, a specific gene of U. virens, as the target for the LF-RPA assay. The designed primers and probe efffectively detected the genomic DNA(gDNA) of U. virens and demonstrated no cross-reactivity with related pathogens. Under optimal conditions, the LF-RPA assay demonstrated a sensitivity of 10 pg of U. virens gDNA. Additionally, by incorporating a simplified PEG-NaOH method for plant DNA extraction, the LF-RPA assay enabled the detection of U. virens in rice spikelets within 30 min, without the need for specialized equipment. Furthermore, the LF-RPA assay successfully detected U. virens in naturally infected rice and seed samples in the field. Therefore, the LF-RPA assay is sensitive, efficient, and convenient, and could be developed as a kit for monitoring rice false smut disease in the field.

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BACNN: Multi-scale feature fusion-based bilinear attention convolutional neural network for wood NIR classification

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Journal of forestry Research 2024年第4期35卷 202-214页

作者： Zihao Wan Hong Yang Jipan Xu Hongbo Mu Dawei Qi college of Science Northeast Forestry UniversityHarbin 150040People’s Republic of China

Effective development and utilization of wood resources is *** modification research has become an integral dimension of wood science research,however,the similarities between modified wood and original wood render it challenging for accurate identification and classification using conventional image classification ***,the development of efficient and accurate wood classification techniques is *** paper presents a one-dimensional,convolutional neural network(i.e.,BACNN)that combines near-infrared spectroscopy and deep learning techniques to classify poplar,tung,and balsa woods,and PVA,nano-silica-sol and PVA-nano silica sol modified woods of *** results show that BACNN achieves an accuracy of 99.3%on the test set,higher than the 52.9%of the BP neural network and 98.7%of Support Vector Machine compared with traditional machine learning methods and deep learning based methods;it is also higher than the 97.6%of LeNet,98.7%of AlexNet and 99.1%of ***,the classification method proposed offers potential applications in wood classification,especially with homogeneous modified wood,and it also provides a basis for subsequent wood properties studies.

关键词： Wood classification Near infrared spectroscopy Bilinear network SE module Anti-noise algorithm

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DIA-based proteome profiling with PRM verification reveals the involvement of ER-associated protein processing in pollen abortion in Ogura CMS cabbage

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Horticultural Plant Journal 2024年第3期10卷 755-770页

作者： Peiwen Wang Lin Zhu Ziheng Li Mozhen Cheng Xiuling Chen Aoxue Wang Chao Wang Xiaoxuan Zhang Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(Northeast Region) Ministry of Agriculture and Rural AffairsNortheast Agricultural UniversityHarbinHeilongjiang 150030China college of Horticulture and landscape architecture Northeast Agricultural UniversityHarbinHeilongjiang 150030China

Ogura cytoplasmic male sterility(Ogura CMS)is extensively applied in hybrid seed production in cruciferous ***,the posttranscriptional molecular basis of Ogura CMS in cruciferous crops remains ***,a data-independent acquisition-based proteomic approach coupled with a parallel reaction monitoring-based targeted proteomic assay was used to analyze the proteome dynamics of Ogura CMS cabbage line RM and its maintainer line RF during floral bud development to obtain insights into the mechanism underlying Ogura CMS in cruciferous crops.A total of 9162 proteins corresponding to 61464 peptides were identified in RM and RF floral *** proteomic fluctuation of RM was weaker than that of *** in protein expression between RM and RF gradually enlarged with floral bud *** continually up-regulated and eight continually down-regulated proteins were found in RM relative to RF throughout floral bud *** expressed proteins between RM and RF during floral bud development were implicated in the endoplasmic reticulum(ER)-associated protein processing pathway,in which most of them exhibited down-regulated expression in *** data suggest that ER-associated protein processing may be involved in pollen abortion in Ogura CMS cabbage by inhibiting the expression of critical *** findings not only deepen the understanding of the molecular mechanisms of Ogura CMS in cruciferous crops but also provide better guidance for applying Ogura CMS in the hybrid breeding of cruciferous crops.

关键词： Ogura cytoplasmic male sterility(Ogura CMS) Cabbage Data-independent acquisition(DIA) Parallel reaction monitoring(PRM) Pollen development

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A study on the impact of heritage protection on urban carbon emissions

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Science China(Technological Sciences) 2024年第9期67卷 2675-2692页

作者： WANG Fang XUE PengCheng WANG ShunYi LIU YunHuai River Basin Habitats Research Center College of Architecture and Landscape ArchitecturePeking UniversityBeijing 100871China Shanxi Key Laboratory of Watershed Built Environment with Locality(Peking University) Peking UniversityBeijing 100871China college of Urban and Environmental Sciences Peking UniversityBeijing 100871China School of Computer Science Peking UniversityBeijing 100871China

A major goal of current international urban development and construction is to lower carbon *** exist between the preservation of historic cities and the development of low-carbon cities;however,the relationship between preservation of historic cities and carbon emissions remains *** study focuses on the cities in the“Yellow-Canal-Yangtze”watershed community,which includes the Yellow River Basin,Yangtze River Basin,and the Grand Canal region,and aims to verify the differences in CO_(2) emissions(CE)between historic and non-historic cities through multiple comparisons after ***,Ridge Regression was used to determine the impact and dimensions of the relevant variables associated with the preservation of historic cities on *** with national development goals,this study predicts changes in the CE of historic city preservation under different *** study found significant differences between historic and non-historic cities,as the total CE of historic cities is 2.42 times higher than that of non-historic *** dimension with the largest difference was the CE related to transportation,with aviation emissions of historic cities reaching 15.22 times higher than that of non-historic *** the preservation of historic cities has led to an overall increase in the CE,there is significant potential for CE reduction in land use,transportation infrastructure,and tourism development related to historic city *** the future,historic cities can contribute to China's carbon neutrality and the United Nation's development goals through the creation of larger historical preservation areas,more convenient public transportation,and more vibrant tourism.

关键词： CO_(2) emissions urban planning cultural heritage historic city “Yellow-Canal-Yangtze”watershed community

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Synthesis and Characterization of Phenyl Camellia oleifera Seed Oil Ester Plasticizing PVC

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Journal of Renewable Materials 2024年第3期12卷 615-628页

作者： Wenqing Xiao Yuhang Liu Yuxin He Qiaoguang Li Yongquan Li college of Chemistry and Chemical Engineering Zhongkai University of Agriculture and EngineeringGuangzhou510225China college of Chemical Engineering Zhejiang University of TechnologyHangzhou310000China Key Laboratory of Germplasm Platform and Ornamental Plants College of Horticulture and Landscape ArchitectureZhongkai University of Agriculture and EngineeringGuangzhou510225China

Plasticizers are essential additives in the processing of polyvinyl chloride(PVC),with phthalate plasticizers being widely ***,these conventional plasticizers have been shown to be harmful to human health and environmentally unfriendly,necessitating the exploration of eco-friendly bio-based *** this study,Camellia oleifera seed oil,a specialty resource in China,was utilized as a raw material and reacted with 4,4′-Methylenebis(N,N-diglycidylaniline)(AG-80)to synthesize Phenyl Camellia seed Oil Ester(PCSOE).PCSOE was employed as a plasticizer to prepare modified PVC films with varying concentrations,with the conventional plasticizer dioctyl phthalate(DOP)serving as a *** results demonstrate that PSCOE-plasticized PVC films exhibit enhanced hydrophilicity,tensile strength,and thermal stability compared to DOP-modified PVC *** contact angle of PSCOE-plasticized PVC films ranges from 66.26°to 78.48°,which is generally lower than the contact angle of DOP-modified PVC films at 78.40°,indicating improved hydrophilicity due to the modification with *** tensile strength of PSCOE-plasticized PVC films ranges from 17.73 to 20.17 MPa,all surpassing the value of 16.41 MPa for DOP-modified PVC ***,the temperatures corresponding to 5%,10%,and 50%weight loss for PVC samples modified with PCSOE are higher than those for ***,PCSOE presents a viable alternative to DOP as a plasticizer for PVC materials.

关键词： Polyvinyl chloride(PVC) Camellia oleifera seed oil 4,4′-Methylenebis(N,N-diglycidylaniline) bio-based plasticizer

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The Identification of Phenylalanine Ammonia-Lyase(PAL)Genes from Pinus yunnanensis and an Analysis of Enzyme Activity in vitro

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Phyton-International Journal of Experimental Botany 2024年第3期93卷 503-516页

作者： Dejin Mu Lin Chen Heze Wang Zhaoliu Hu Sihui Chen Shi Chen Nianhui Cai Yulan Xu Junrong Tang Key Laboratory of National forestry and Grassland Administration on Biodiversity Conservation in Southwest China Southwest Forestry UniversityKunming650224China college of forestry Southwest Forestry UniversityKunming650224China

Phenylalanine ammonia lyase(PAL)is the rate-limiting and pivotal enzyme of the general phenylpropanoid path-way,but few reports have been found on PAL genes in Pinus *** the present study,three PAL genes were cloned and identiﬁed from *** seedlings for theﬁrst time,namely,PyPAL-1,PyPAL-2,and *** results indicated that the open-reading frames of PyPAL genes were 2184,2157,and 2385 *** tree analysis revealed that PyPALs have high homology with other known PAL genes in other *** vitro enzymatic analysis showed that all three PyPAL recombinant proteins could catalyze the deamination of L-phenylalanine to form trans-cinnamic acid,but only PAL1 and PAL2 can catalyze the conversion of L-tyrosine toρ-coumaric *** PyPAL genes were expressed in different tissues in 1-year-old ***,and such genes had different expression *** study lays a foundation for further understanding of the biosynthesis of secondary metabolites in ***.

关键词： Pinus yunnanensis phenylalanine ammonia-lyase enzyme activity in vitro functional analysis secondary metabolites

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Flavonoid extracts from chrysanthemum with appropriate anthocyanins turn blue when exposed to iron ions

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Horticultural Plant Journal 2024年第3期10卷 837-852页

作者： Yanfei Li Jiaying Wang Chenfei Lu Zhongman Wang Chengyan Deng Kang Gao Jingjing Li Zhijun Fang Hao Liu Yan Hong Silan Dai Beijing Key Laboratory of Ornamental Plants Germplasm Innovation&Molecular Breeding National Engineering Research Center for FloricultureBeijingLaboratory of Urban and Rural Ecological EnvironmentKey Laboratory for Genetics and Breeding of Forest Trees and Ornamental Plants of Ministry of EducationSchool of Landscape ArchitectureBeijing Forestry UniversityBeijing 100083China Beijing Greenland Maintenance and Management Service Center Beijing 102211China Beijing North Asia landscape Co. Beijing 102211China

Although some species that accumulate only cyanidin(Cy)in nature can produce blue flowers through iron ions,there has been no evidence of blue chrysanthemums being generated in this *** study revealed that flavonoid extracts from the ray florets of the chrysanthemum cultivar‘Wandai Fengguang’turned blue when exposed to Fe^(3+).Samples that could turn blue were labeled as CB(Cy-determined blue flowers),while samples that did not turn blue were labeled as CN(Cy-determined non-blue flowers).After a series of experiments,a stable screening system was established using flavonoid extracts containing NaAc buffer at pH 5.5 and a total anthocyanin concentration(TAC)of 30 μmol·L^(-1),and the addition of Fe^(3+)from 0 to 0.25 μmol·L^(-1)allowed for the selection of five CB samples from 39 chrysanthemum *** five CB samples exhibited flower color phenotypes that belonged to Cluster-I with redness(a*)values ranging from 29.03 to 45.99,yellowness(b*)values from-11.31 to 3.77,and brightness(L*)values from 29.07 to ***,the ratio of TAC to total luteolin concentration(TLC)was found to be a critical factor for distinguishing between CB and CN *** realize the desired blue hue in the flavonoid extracts with the participation of Fe^(3+),a TAC to TLC ratio of 2.25 and above is ***,the protoplasts and ray florets of CB samples that turned blue with the involvement of Fe^(2+)showed great potential for cultivating blue chrysanthemums through ferric-anthocyanin ***,this study reveals that blue flowers can be cultivated through the increase in the iron ion concentration,combined with the accumulation of Cy.

关键词： Chrysanthemum×morifolium Cyanidin Copigment Ferric-anthocyanin chelate Blue flower

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Old Pinus massoniana forests benefit more from recent rapid warming in humid subtropical areas of central-southern China

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Journal of forestry Research 2024年第5期35卷 155-170页

作者： Wenxin Li Liangjun Zhu Lianhua Zhu Mengdan Jing Censhi Qian Yu Zhu Paolo Cherubini college of Life Science and Technology Central South University of Forestry and TechnologyChangsha 410004People’s Republic of China Swiss Federal Institute for Forest Snow and Landscape Research WSL8903 BirmensdorfSwitzerland Department of Forest and Conservation Sciences Faculty of ForestryUniversity of British Columbia2004-2424 Main MallVancouverBC V6T 1Z4Canada

Trees progress through various growth stages,each marked by specific responses and adaptation strate-gies to environmental *** the importance of age-related growth responses on overall forest health and management policies,limited knowledge exists regarding age-related effects on dendroclimatic relationships in key subtropical tree *** this study,we employed a den-drochronological method to examine the impact of rapid warming on growth dynamics and climatic sensitivity of young(40–60 years)and old(100–180 years)Pinus mas-soniana forests across six sites in central-southern *** normalized log basal area increment of trees in both age groups increased significantly following rapid warming in *** in young forests further showed a distinct growth decline during a prolonged severe drought(2004–2013),whereas those in old forests maintained growth *** growth was more strongly influenced by temperature than by moisture,particularly in old *** tem-peratures strongly and positively impacted the growth of old trees but had a weaker effect on young *** forests had a significantly lower resistance to extreme drought but faster recovery compared to young ***“divergence problem”was more pronounced in younger forests due to their heightened sensitivity to warming-induced drought and heat *** ongoing warming,young forests also may initially experience a growth decline due to their heightened sensitivity to winter *** findings underscore the importance of considering age-dependent changes in forest/tree growth response to warming in subtropical forest man-agement,particularly in the context of achieving“Carbon Peak&Carbon Neutrality”goals in China.

关键词： Tree rings Pinus massoniana Age effects Drought resilience Subtropical forests Rapid warming

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Endoplasmic reticulum stress-induced NLRP3 inflammasome activation as a novel mechanism of polystyrene microplastics (PS-MPs)-induced pulmonary inflammation in chickens

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2024年第3期25卷 233-243页

作者： Hongmin LU Tiantian GUO Yue ZHANG Dewang LIU Lulu HOU Chengxue MA Mingwei XING college of Wildlife and Protected Area Northeast Forestry UniversityHarbin 150040China

Microplastics(MPs)have attracted growing attention worldwide as an increasingly prevalent environmental *** addition,chicken meat is currently the most widely consumed kind of poultry in the global *** demand for chicken is on the rise both at home and *** a result,the safety of chicken raising has also received significant *** lungs play an essential role in the physiological activities of chickens,and they are also the most vulnerable *** injury is difficult to repair after the accumulation of contaminants,and the mortality rate is high,which brings huge economic losses to *** research on the toxicity of MPs has mainly focused on the marine ecosystem,while the mechanisms of toxicity and lung damage in chickens have been poorly ***,this study explored the effects of exposure to polystyrene microplastics(PS-MPs)at various concentrations for 42 d on chicken ***-MPs could cause lung pathologies and ultrastructural abnormalities,such as endoplasmic reticulum(ER)swelling,inflammatory cell infiltration,chromatin agglutination,and plasma membrane ***,PS-MPs increased the expression of genes related to the heat shock protein family(Hsp60,Hsp70,and Hsp90),ER stress signaling(activating) transcription factor 6(ATF0),ATF4,protein kinase RNA-like ER kinase(PERK),and eukaryotic translation initiation factor 2 subunitα(eIF2a),pyroptosis-related genes(NOD)-,LRR-and pyrin domain-containing protein 3(NLRP3),apoptosis-associated speck-like protein containing a caspase recruitment domain(ASC),interleukin-1β(IL-1β),cysteinyl aspartate-specific proteinase 1(Caspasel),and gasdermin-D(GSDMD),and the inflammatory signaling pathway(nuclear factor-kB(NF-kB),inducible nitric oxide synthase(iNOS),and cyclooxygenase-2(COX-2).The above results showed that PS-MP exposure could result in lung stress,ER stress,pyroptosis,and inflammation in *** findings provide new scientific clues for further research o

关键词： Polystyrene microplastics Endoplasmic reticulum stress Lung NLRP3 inflammasome Inflammation

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Strategies and Economic Benefit Analysis for Productive landscape of Rape Flower Sea in Cold Regions

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Journal of Northeast Agricultural University(English Edition) 2024年第2期31卷 34-42页

作者： Zhang Jiaxin Wu Zhiheng Zhu Xuanbo Pan Shengkai Yan Yongqing college of Horticulture and landscape architecture Northeast Agricultural UniversityHarbin 150030China

The cold regions are located in high latitudes and cold *** local excellent ornamental plant resources are relatively *** plant species that are suitable for both ornamental and productive benefits of landscape of flower sea construction are also even ***,it is imperative to introduce and screen the plant resources suitable for cold regions to create the landscape of flower *** rape,an oilseed crop,was used as a research object in order to create a productive flower landscape with both ornamental and economic values in cold *** rape flower varieties,Qingza No.5,7,9,and 11,were introduced from Qinghai Hufeng Agricultural Science and Technology Group Co.,*** were planted in the experimental practice base of Northeast Agricultural University in three *** characteristics and seed yield of rape flowers on different sowing dates were *** fuzzy probability method was used to comprehensively evaluate the *** results showed that the rape flowers grew well in Harbin City during the experimental sowing period,which could form a good landscape of flower sea and had a considerable rapeseed *** could be widely used in cold urban and rural areas,such as Harbin *** view of the experimental results,the strategies of creating a productive landscape of rape flower sea were proposed and the economic benefits were *** could change the status quo of a uniform landscape of flower sea in cold regions,help the development of rural tourism,and promote local economic income.

关键词： rape flower sea productive landscape cold region landscape construction economic benefit

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