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The influence of salinity on toxicological effects of arsenic in digestive gland of clam Ruditapes philippinarum using metabolomics

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Chinese Journal of Oceanology and Limnology 2013年第2期31卷 345-352页

作者：吉成龙吴惠丰刘小莉赵建民于君宝尹秀丽 key laboratory of coastal Zone environmental processes Yantai Institute of Coastal Zone Research(YIC)Chinese Academy of Sciences(CAS) shandong provincial key laboratory of coastal Zone environmental processes Graduate University of Chinese Academy of Sciences

Ruditapes philippinarum,a clam that thrives in intertidal zones of various salinities,is a useful biomonitor to marine *** investigated the influence of dilution to 75% and 50% of normal seawater salinity(31.1) on the responses of the digestive gland of *** to arsenic exposure(20 μg/L),using nuclear magnetic resonance(NMR)-based *** acute arsenic exposure for 48 h,salinity-dependent differential metabolic responses were *** normal seawater,arsenic exposure increased the concentrations of branched-chain amino acids,and of threonine,proline,phosphocholine and adenosine,and it decreased the levels of alanine,hypotaurine,glucose,glycogen and ATP in the digestive *** changes in metabolic biomarkers observed at lower salinity(～23.3) included elevation of succinate,taurine and ATP,and depletion of branched-chain amino acids,threonine and *** effects of arsenic at the lowest salinity(～15.6) included down-regulation of glutamate,succinate and ADP,and up-regulation of *** conclude that salinity influences the metabolic responses of this clam to arsenic.

关键词： Ruditapes philippinarum salinity arsenic toxicological effects nuclear magnetic resonance(NMR) metabolomics

Molecular cloning and differential expression patterns of sigma and omega glutathione S-transferases from Venerupis philippinarum to heavy metals and benzo[a]pyrene exposure

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Chinese Journal of Oceanology and Limnology 2012年第3期30卷 413-423页

作者：张林宝吴惠丰刘小莉陈磊磊王清赵建民由丽萍 key laboratory of coastal Zone environmental processes Yantai Institute of Coastal Zone Research(YIC)Chinese Academy of Sciences(CAS) shandong provincial key laboratory of coastal Zone environmental processes YICCAS Graduate University of Chinese Academy of Sciences

Glutathione S-transferases (GSTs) are a class of enzymes that facilitate the detoxification of xenobiotics, and also play important roles in antioxidant defense. We identified two glutathione S-transferase isoforms (VpGSTS, sigma GST; VpGSTO, omega GST) from Venerupis philippinarum by RACE approaches. The open reading frames of VpGSTS and VpGSTO were of 612 bp and 729 bp, encoding 203 and 242 amino acids with an estimated molecular mass of 22.88 and 27.94 kDa, respectively. The expression profiles of VpGSTS and VpGSTO responded to heavy metals and benzo[a]pyrene (B[a]P) exposure were investigated by quantitative real-time RT-PCR. The expression of VpGSTS and VpGSTO were both rapidly up-regulated, however, they showed differential expression patterns to different toxicants. Cd displayed stronger induction of VpGSTS expression with an approximately 12-fold increase than that of VpGSTO with a maximum 6.4-fold rise. Cu exposure resulted in similar expression patterns for both VpGSTS and VpGSTO. For B[a]P exposure, the maximum induction of VpGSTO was approximately two times higher than that of VpGSTS. Altogether, these findings implied the involvement of VpGSTS and VpGSTO in host antioxidant responses, and highlighted their potential as a biomarker to Cd and B[a]P exposure.

关键词： Venerupis philippinarum Glutathione S-transferase mRNA expression heavy metals benzo[a]pyrene biomarker

Macroalgae Blooms and their Effects on Seagrass Ecosystems

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Journal of Ocean University of China 2014年第5期13卷 791-798页

作者： HAN Qiuying LIU Dongyan key laboratory of coastal Zone environmental processes and Ecological Remediation CAS Shandong Provincial Key Laboratory of Coastal Zone Environmental Processes CAS Experimental Station of Integrated Coastal Environment in Muping Yantai Institute of Coastal Zone Research (YIC) Chinese Academy of Sciences

Seagrass decline caused by the macroalgae blooms is becoming a common phenomenon throughout temperate and tropical regions. We summarized the incidence of macroalgae blooms throughout the world and their impact on seagrass beds by direct and indirect ways. The competition for living space and using resources is the most direct effect on seagrass beds when macroalgae are blooming in an aquatic ecosystem. The consequence of macroalgae blooms(e.g., light reduction, hypoxia, and decomposition) can produce significant indirect effects on seagrass beds. Light reduction by the macroalgae can decrease the growth and recruitment of seagrasses, and decomposition of macroalgae mats can increase the anoxic and eutrophic conditions, which can further constrict the seagrass growth. Meanwhile, the presence of seagrass shoots can provide substrate for the macroalgae blooms. Controlling nutrient sources from the land to coastal waters is a general efficient way for coastal management. Researching into the synergistical effect of climate change and anthropognic nutrient loads on the interaction between searsasses and macroalgae can provide valuable information to decrease the negative effects of macroalgae blooms on seagrasses in eutrophic areas.

关键词： eutrophication decline seagrasses macroalgae blooms

The relationship between soft-bottom macrobenthic communities and environmental variables off Ningjin,eastern shandong Peninsula

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Acta Oceanologica Sinica 2010年第6期29卷 73-82页

作者： LI Baoquan LI Xinzheng WANG Hongfa WANG Jinbao HAN Qingxi key laboratory of coastal Zone environmental processes CAS shandong provincial key laboratory of coastal Zone environmental processes Yantai Institute of coastal Zone Research Chinese Academy of Sciences Yantai 264003 China Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China

Macrobenthic infauna and associated environmental factors influencing the benthic community in the eastern coastal region of shandong Peninsula were analyzed in four seasonal surveys from January 2007 to October 2007 （30 stations in winter,20 stations in other three seasons）,in order to understand the community structure and the factors unfluencing the benthic *** 6.0 and SPSS 15.0 software packages were adopted to analyze the environmental and macrobenthic *** results show that there were 260 macrobenthic species in total collected from the research *** composition of species is： Polychaeta （94 species）,Crustacea （75）,Mollusca （56） and Echinoderm （12）,among which,only 23 species were common species in the cruises of every *** dominant species varied from season to season;however,the polychaete species Paralacydonia paradoxa Fauvel and Echinoderm species Amphioplus japonicus （Matsumoto） were always present *** abundance and biomass of the macrobenthos in the research region were variable from season to *** results of CLUSTER and MDS analysis show that the similarities of macrobenthic structures between the stations were low;most of the similarities were at about 30% of similarity value,only two stations were up to 70%.In accordance with the similarity values of the macrobenthic structures,we divided the 20 stations into five groups by arbitrary similarity level of 30%.The ABC curve indicates that the marcofauna communities in the research region had not been disturbed massively,except two stations,SB1 and ***,benthos were controlled by a combination of factors such as salinity,phytoplankton,zooplankton,SiO 3Si and temperature,and no single factor could be considered as an ecological master factor.

关键词： benthos community structure biodiversity the Huanghai (Yellow) Sea shandong Peninsula

Homologous cloning, characterization and expression of a new halophyte phytochelatin synthase gene in Suaeda salsa

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Chinese Journal of Oceanology and Limnology 2016年第5期34卷 1034-1043页

作者：丛明赵建民吕家森任志明吴惠丰 key laboratory of coastal Zone environmental processes Yantai Institute of Coastal Zone Research(YIC)Chinese Academy of Sciences(CAS)Shandong Provincial Key Laboratory of Coastal Zone Environmental ProcessesYICCASYantai 264003China Biology School of Yantai University Yantai 264005China

The halophyte Suaeda salsa can grow in heavy metal-polluted areas along intertidal zones having high *** phytochelatins can effectively chelate heavy metals,it was hypothesized that *** possessed a phytochelatin synthase(PCS) *** the present study,the cDNA of PCS was obtained from ***(designated as SsPCS) using homologous cloning and the rapid amplification of cDNA ends(RACE).A sequence analysis revealed that SsPCS consisted of 1 916 bp nucleotides,encoding a polypeptide of 492 amino acids with one phytochelatin domain and one phytochelatin C domain.A similarity analysis suggested that SsPCS shared up to a 58.6%identity with other PCS proteins and clustered with PCS proteins from *** was a new kind of metal ion sensor motif in its C-terminal *** SsPCS transcript was more highly expressed in elongated and fibered roots and stems(P<0.05) than in *** and mercury exposure significantly enhanced the mRNA expression of SsPCS(P<0.05).To the best of our knowledge,SsPCS is the second PCS gene cloned from a halophyte,and it might contain a different metal sensing capability than the first PCS from Thellungiella *** study provided a new view of halophyte PCS genes in heavy metal tolerance.

关键词： Suaeda salsa halophyte phytoehelatin synthase (PCS) homologous cloning heavy metal tissue distribution

Anthropogenic impacts on hyperbenthos in the coastal waters of Sishili Bay,Yellow Sea

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Chinese Journal of Oceanology and Limnology 2013年第6期31卷 1257-1267页

作者：李宝泉 John K.KEESING 刘东艳韩庆喜王玉珏董志军陈桥 key laboratory of coastal Zone environmental processes CAS shandong provincial key laboratory of coastal Zone environmental processes Yantai Institute of coastal Zone Research Chinese Academy of Sciences CSIRO Marine and Atmospheric Research Private Bag 5Wembley 6913Australia College of Geological Science & Engineering Shandong University of Science and Technology

A survey on the hyperbenthic communities was carried out in the coastal waters of Sishili Bay in the Yellow Sea in July 2009,to investigate the impact of putative anthropogenic activities related to the presence of a sewage outfall,a harbor and an aquaculture site on the benthic *** Agassiz net trawl was used to collect hyperbenthos at 10 sampling ***,diversity,abundance,and biomass were analyzed against water sample data and historical data obtained from records from nearby Yantai ***-two species were identified in the region,of which Crustacea were the most abundant taxon,followed by *** species included five crustaceans,two echinoderms,one mollusk and one fish *** results of a BIOENV analysis show that the concentrations of NO3-N and Chl-a were slightly positively correlated with hyperbenthic community structure,while other factors were negatively correlated,including sediment grain size and the percentage of TN and TOC in ***- Biomass Comparison(ABC method) curves indicate that the hyperbenthos in Sishili Bay had been disturbed by putative sources of human *** out of 10 stations were classified as "moderately perturbed" to "perturbed".

关键词： hyperbenthos suprabenthos biodiversity Northem Yellow Sea anthropogenic activity

Impact of tropical cyclone Matmo on mixed zone of the Yellow and Bohai Seas

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Journal of Oceanology and Limnology 2018年第5期36卷 1484-1493页

作者： GUO Jie JI Diansheng HOU Chawei GUO Kai JI Ling Yantai Institute of coastal Zone Research Chinese A cademy of SciencesKey Laboratory of Coastal Environmental Processes and Ecological RemediationCAS shandong provincial key laboratory of coastal environmental processes Yantai 264003China 2 YantaiMarine environmentalMonitoring Central Station State Oceanic Administration（SOA）Yantai 264006China

The Bohai Sea is a low-lying semi-enclosed sea area that is linked to the Yellow Sea via the Bohai straits(mixed zone). Its of fshore seabed is shallow, which makes it vulnerable to serious marine meteorological disasters associated with the northward passage of Pacific tropical cyclones. Analyses on data of remote sensing and buoy of the mixed zone of the Yellow and Bohai seas indicate that all the wind speed, significant wave height, and salinity(SAL) increased, sea surface temperature decreased, and wind energy density changed considerably during the passage of tropical cyclone Matmo on July 25, 2014. It was found that the SAL inversion layer in the mixed zone of the Yellow and Bohai Seas was caused by the tropical cyclone. Furthermore, it was found that the tropical cyclone transported the northern Yellow Sea cold water mass(NYSCWM) into the mixed zone of the Yellow and Bohai Seas. The NYSCWM has direct influence on both the aquaculture and the ecological environment of the region. Therefore, further research is needed to establish the mechanism behind the formation of the SAL inversion layer in the mixed zone, and to determine the influence of tropical cyclones on the NYSCWM.

关键词： Yellow and Bohai seas mixed zone northern Yellow Sea cold water mass wind speed sea surface temperature salinity

Wave steepness retrieved from scatterometer data in a genetic algorithm

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Chinese Journal of Oceanology and Limnology 2012年第2期30卷 336-341页

作者：过杰何宜军 key laboratory of coastal Zone environmental processes Yantai Institute of Coastal Zone Research(YIC)Chinese Academy of Sciences(CAS) shandong provincial key laboratory of coastal Zone environmental processes YICCAS Nanjing University of Information Science and Technology School of Marine Sciences key laboratory of Ocean Circulation and Waves Institute of OceanologyChinese Academy of Sciences

Wave steepness is an important characteristic of a high sea state, and is widely applied on wave propagations at ports, ships, offshore platforms, and CO2 circulation in the ocean. Obtaining wave steepness is a difficult task that depends heavily on theoretical research on wavelength distribution and direct observations. Development of remote-sensing techniques provides new opportunities to study wave steepness. At present, two formulas are proposed to estimate wave steepness from QuikSCAT and ERS-1/2 scatterometer data. We found that wave steepness retrieving is not affected by radar band, and polarization method, and that relationship of wave steepness with radar backscattering cross section is similar to that with wind. Therefore, we adopted and modified a genetic algorithm for relating wave steepness with radar backscattering cross section. Results show that the root-mean-square error of the wave steepness retrieved is 0.005 in two cases from ERS-1/2 scatterometer data and from QuikSCAT scatterometer data.

关键词： wave steepness genetic algorithm scatterometer data buoy data

Manila clam Venerupis philippinarum as a biomonitor to metal pollution

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Chinese Journal of Oceanology and Limnology 2013年第1期31卷 65-74页

作者：吴惠丰吉成龙王清刘小莉赵建民冯江华 key laboratory of coastal Zone environmental processes Yantai Institute of Coastal Zone Research (YIC)Chinese Academy of Sciences (CAS) shandong provincial key laboratory of coastal Zone environmental processes YICCAS Graduate University of Chinese Academy of Sciences Department of Electronic Science Fujian Key Laboratory of Plasma and Magnetic ResonanceState Key Laboratory of Physical Chemistry of Solid SurfacesXiamen University

The Manila clam Venerupis philippinarum is a good biomonitor/bioindicator to marine metal pollution and is frequently used in aquatic toxicology. Two dominant pedigrees (white and zebra) of clam are distributed in the Bohai Sea; however, little attention has been paid to potential biological differences between these two pedigrees. In this study, we tested the sensitivity of both pedigrees to marine metal (cadmium and zinc) pollution biomonitoring and marine environmental toxicology. Results demonstrate significant biological differences in gills of white and zebra clams based on metabolic profiles and antioxidant enzyme activities. In addition, we found that hypotaurine, malonate and homarine were relatively high in white clam gills, while alanine, arginine, glutamate, succinate, 4-aminobutyrate, taurine and betaine were high in zebra clam gills. Zebra clam gills were also more sensitive to a mixture of Cd and Zn, as shown by antioxidant enzyme activities and metabolic profiles, but white clam gills could accumulate more Zn. Therefore, we suggest that the white pedigree can be used as a biomonitor to marine Zn pollution, whereas the zebra pedigree can be used for toxicology studies on Cd and Zn mixed pollution.

关键词： Manila clam Venerupisphilippinarum biomonitor biomarker metabolomics