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Development and characterization of bacterial wilt-resistant synthetic polyploid peanuts

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Crop Journal 2025年第1期13卷 125-134页

作者： Du, Pei Zeng, Fanpei Wang, Qian Miao, Lijuan Qi, Feiyan Yang, Meili Wang, Xiao Liu, Hua Chen, Guoquan Fu, Liuyang Han, Suoyi Sun, Ziqi Qin, Li Dong, Wenzhao Zhang, Xinyou the shennong laboratory/Nation Industrial Innovation Centre for Bio-Breeding/Key laboratory of Oil Crops in Huang-Huai-Hai Plains Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crops Improvement/Institute of Crop Molecular Breeding Henan Academy of Agricultural Sciences Postgraduate T&R Base of Zhengzhou University Henan Zhengzhou 450002 China School of Agricultural Sciences Zhengzhou University Henan Zhengzhou 450001 China School of Life Science Zhengzhou University Henan Zhengzhou 450001 China Hebi Academy of Agricultural Sciences Henan Hebei 458031 China

Wild peanut (Arachis) species are promising sources of disease resistance for improving peanut cultivars. The objective of this study was to assess cross-compatibility among cultivated and wild peanuts in crosses between eight peanut cultivars and 27 wild species carrying the A, B, E, Ex, F, K, P, and H genomes. Embryo culture and chromosome doubling led to polyploids representing hybrids between cultivated peanut and A. stenosperma, A. macedoi, A. duranensis, A. villosa, and A. diogoi. The first two showed greater resistance to bacterial wilt than their cultivated parents. DNA markers were developed for verifying the hybrids and for identifying translocation or introgression lines with alien chromosome fragments. © 2025 Crop Science Society of China and Institute of Crop Science, CAAS

关键词： Bacterial wilt Cross compatibility Peanut Polyploids Wild species

The chimeric gene atp6c confers cytoplasmic male sterility in maize by impairing the assembly of the mitochondrial ATP synthase complex

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Molecular Plant 2022年第5期15卷 872-886页

作者： Huili Yang Yadong Xue Bing Li Yanan Lin Haochuan Li Zhanyong Guo Weihua Li Zhiyuan Fu Dong Ding Jihua Tang National Key laboratory of Wheat and Maize Crop Science College of AgronomyHenan Agricultural UniversityZhengzhouChina the shennong laboratory ZhengzhouChina

Cytoplasmic male sterility(CMS)is a powerful tool for the exploitation of hybrid heterosis and the study of signaling and interactions between the nucleus and the cytoplasm.C-type CMS(CMS-C)in maize has long been used in hybrid seed production,but the underlying sterility factor and its mechanism of action remain *** this study,we demonstrate that the mitochondrial gene atp6c confers male sterility in CMS-C *** ATP6C protein shows stronger interactions with ATP8 and ATP9 than ATP6 during the assembly of F1F0-ATP synthase(F-type ATP synthase,ATPase),thereby reducing the quantity and activity of assem-bled F_(1)F_(o)-ATP *** contrast,the quantity and activity of the F1'component are increased in CMS-C *** F1F0-ATP synthase activity causes accumulation of excess protons in the inner membrane space of the mitochondria,triggering a burst of reactive oxygen species(ROS),premature programmed cell death of the tapetai cells,and pollen ***,our study identifies a chimeric mitochondrial gene(ATP6C)that causes CMS in maize and documents the contribution of ATP6C to F1F0-ATP synthase assembly,thereby providing novel insights into the molecular mechanisms of male sterility in plants.

关键词： maize cytoplasmic male sterility atp6c ATP synthase mitochondria

Assessing the conservation impact of Chinese indigenous chicken populations between ex-situ and in-situ using genome-wide SNPs

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Journal of Integrative Agriculture 2024年第3期23卷 975-987页

作者： Wenting Li Chaoqun Gao Zhao Cai Sensen Yan Yanru Lei Mengya Wei Guirong Sun Yadong Tian Kejun Wang Xiangtao Kang College of Animal Science and Technology Henan Agricultural UniversityZhengzhou 450002China the shennong laboratory Zhengzhou 450002China

Conservation programs require rigorous evaluation to ensure the preservation of genetic diversity and viability of conservation populations. In this study, we conducted a comparative analysis of two indigenous Chinese chicken breeds, Gushi and Xichuan black-bone, using whole-genome SNPs to understand their genetic diversity, track changes over time and population structure. The breeds were divided into five conservation populations(GS1, 2010, ex-situ;GS2, 2019, ex-situ;GS3, 2019, in-situ;XB1, 2010, in-situ;and XB2, 2019, in-situ) based on conservation methods and generations. The genetic diversity indices of three conservation populations of Gushi chicken showed consistent trends, with the GS3 population under in-situ strategy having the highest diversity and GS2 under ex-situ strategy having the lowest. The degree of inbreeding of GS2 was higher than that of GS1 and GS3. Conserved populations of Xichuan black-bone chicken showed no obvious changes in genetic diversity between XB1 and XB2. In terms of population structure, the GS3 population were stratified relative to GS1 and GS2. According to the conservation priority, GS3 had the highest contribution to the total gene and allelic diversity in GS breed, whereas the contribution of XB1 and XB2 were similar. We also observed that the genetic diversity of GS2 was lower than GS3, which were from the same generation but under different conservation programs(in-situ and ex-situ). While XB1 and XB2 had similar levels of genetic diversity. Overall, our findings suggested that the conservation programs performed in ex-situ could slow down the occurrence of inbreeding events, but could not entirely prevent the loss of genetic diversity when the conserved population size was small, while in-situ conservation populations with large population size could maintain a relative high level of genetic diversity.

关键词： genome-wide SNPs conservation genetic diversity ex-situ in-situ

Regulation of maize growth and immunity by ZmSKI3-mediated RNA decay and post-transcriptional gene silencing

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Journal of Integrative Plant Biology 2024年第11期66卷 2561-2577页

作者： Jie Gao Na Zhang Guohui Liu Jinjun Tian Mengyao Chen Ying Wang Ye Xing Ying Zhang Chenyang Zhao Xiaohuan Mu Yanwen Yu Hongbin Niu Jiankun Li Jihua Tang Mingyue Gou State Key laboratory of Wheat and Maize Crop Science Collaborative Innovation Center of Henan Grain CropsCollege of AgronomyHenan Agricultural UniversityZhengzhou 450002China the shennong laboratory Zhengzhou 450002China

Disease resistance is often associated with compromised plant growth and yield due to defense-growth ***,key components and mechanisms underlying the defensegrowth tradeoffs are rarely explored in *** this study,we find that ZmSKI3,a putative subunit of the SUPERKILLER(SKI)complex that mediates the 3′-5′degradation of RNA,regulates both plant development and disease resistance in *** Zmski3 mutants showed retarded plant growth and constitutively activated defense responses,while the ZmSKI3 overexpression lines are more susceptible to Curvularia lunata and Bipolaris ***,the expression of defense-related genes was generally up-regulated,while expressions of growth-related genes were mostly down-regulated in leaves of the Zmski3-1 mutant compared to that of wild *** addition,223 differentially expressed genes that are up-regulated in Zmski3-1 mutant but down-regulated in the ZmSKI3 overexpression line are identified as potential target genes of ***,small interfering RNAs targeting the transcripts of the defense-and growth-related genes are differentially accumulated,likely to combat the increase of defense-related transcripts but decrease of growthrelated transcripts in Zmski3-1 *** together,our study indicates that plant growth and immunity could be regulated by both ZmSKI3-mediated RNA decay and post-transcriptional gene silencing in maize.

关键词： defense-growth tradeoffs disease resistance maize plant growth PTGS RNA decay ZmSKI3

Deletion of a 1,049 bp sequence from the 5′UTR upstream of the SiHEC3 gene induces a seed non-shattering mutation in sesame

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Journal of Integrative Agriculture 2024年第8期23卷 2589-2604页

作者： Ming Ju Guiting Li Qiuzhen Tian Hengchun Cao Qin Ma Yinghui Duan Hui Guo Zhanyou Zhang Yingying Huang Huili Wang Haiyang Zhang Hongmei Miao the shennong laboratory Zhengzhou 450002China Henan Sesame Research Center Henan Academy of Agricultural SciencesZhengzhou 450002China Key laboratory of Specific Oilseed Crops Genomics of Henan Province Henan Sesame Research CenterHenan Academy of Agricultural SciencesZhengzhou 450002China

Sesame is a labor intensive crop with limited mechanized harvesting mainly due to the seed shattering(SS)*** this study,we performed a genetic analysis of the seed-shattering resistance(SR)trait with a SR sesame mutant *** the SS type,the parenchyma cells in the abscission zone of the 12M07 mutant are arranged loosely but adhere to the seed *** analysis of six generations derived from 12M07(SR)×Xiangcheng Dazibai(SS)showed that the SR trait is recessive and controlled by a single gene *** mapping of the F2population with 888,619 variants(single-nucleotide polymorphisms(SNPs)and insertion-deletion(InDels))and 31,884 structural variations(SVs)determined that only SV12002 in the 5′upstream region of gene Sindi0765000(named SiHEC3)in Chr.3 was significantly associated with the SR ***3 encodes the bHLH transcription factor.A 1,049 bp deletion occurred in the 5′UTR of Sihec3 in ***3 is mainly expressed in developing placental tissues,with the expression peaking in capsules at 45 days after pollination.A dual-luciferase reporter assay in tobacco confirmed that the promoter activity of Sihec3 was reduced because of the deletion of the 1,049 bp promoter ***–protein interaction network analysis showed that HEC3 is co-expressed with nine key proteins,such as SHATTERPROOF1(SHP1)and SEEDSTICK(STK)which participate in the secondary wall biosynthesis of the abscission layer in *** findings of this study show the important function of Sihec3corresponding with the SR trait and supply the genetic information for breeding new varieties that are amenable to mechanized harvesting in sesame and other crops.

关键词： sesame(Sesamum indicum L.) association mapping seed shattering structure variation bHLH transcription factor

Are the two polymorphic sites of anti-Marek’s disease in White Leghorn chickens also suitable for Partridge Shank chickens?

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BIOCELL 2023年第5期47卷 1127-1134页

作者： WENQING LI WANLI LI BEI WANG LIN ZHANG SHENGLI LI CHENWAN LI PINHUI WU GUOQING YANG GUOZHI ZHANG Animal Genetic Engineering laboratory College of Life ScienceHenan Agricultural UniversityZhengzhou450002China shennong laboratory Zhengzhou450002China Institute of Animal Husbandry and Veterinary Sciences Henan Academy of Agricultural SciencesZhengzhou450002China

Background:The selection of Marek’s disease(MD)-resistant breeds in Partridge Shank chicken,a popular local chicken breed in Henan Province of China,has practical *** hypothesized that the two polymorphic sites(rs14527240 located in SMOC1 and GGaluGA156129 located in PTPN3)related to MD resistance in White Leghorn chickens are also applicable to Partridge Shank ***:In this experiment,we screened 10 live hens and 2 live roosters with the double GG genotype by genotyping the two sites from 6500 Partridge Shank *** one-day-old chicks with the double GG genotype were obtained by artificial ***-two one-day-old chickens(19 from the population expansion test and 53 randomly selected from chicken farms)were injected with 2000 plaque-forming units of the Md5 virus *** 100 days of infection,all chickens were examined by pathological anatomical examination,histological sectioning,genotyping,and a quantitative polymerase chain reaction of SMOC1 and ***:There was only one site(rs14527240 located in SMOC1)associated with MD in Partridge Shank chickens(p<0.05),but the GG genotype of SMOC1 in Partridge Shank chickens indicated susceptibility to ***1 expression in MD-susceptible chickens was also significantly higher than that in MDresistant chickens(p<0.05).Conclusion:Therefore,the MD resistance sites selected from White Leghorn chickens were not completely suitable for Partridge Shank chickens,but they can be used as a *** study indicated that SMOC1 plays an important role in screening for MD resistance in poultry.

关键词： Partridge Shank chicken Genotype Marek’s disease MD-resistant chicken

Identification of Fusarium wilt resistance gene SiRLK1 in Sesamum indicum L.

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The Crop Journal 2024年第1期12卷 252-261页

作者： Yinghui Duan Wenwen Qu Shuxian Chang Ming Ju Cuiying Wang Cong Mu Hengchun Cao Guiting Li Qiuzhen Tian Qin Ma Zhanyou Zhang Haiyang Zhang Hongmei Miao the shennong laboratory Zhengzhou 450002HenanChina Henan Sesame Research Center Henan Academy of Agricultural SciencesZhengzhou 450002HenanChina Key laboratory of Specific Oilseed Crops Genomics of Henan Province Henan Sesame Research CenterHenan Academy of Agricultural SciencesZhengzhou 450002HenanChina

Sesame Fusarium wilt(SFW),caused by Fusarium oxysporum ***(Fos),is one of the most devastating diseases affecting sesame *** the genetic control of SFW resistance is pivotal for effective disease management in *** inheritance study on a cross between the highly resistant variety Yuzhi 11 and the highly susceptible accession Sp1 using a Fos pathogenicity group 1 isolate indicated that resistance was conferred by a single dominant *** target locus was located in a 1.24 Mb interval on chromosome 3 using a combination of cross-population association mapping and bulked segregant *** genetic mapping further narrowed the interval between 21,350 and 21,401 *** locus Sindi_0812400 was identified as the SFW resistance gene and officially designated *** gene encodes a specific malectin/receptor-like protein kinase with three putative tandem kinase domains and is considered a kinase fusion *** analysis revealed that a high proportion(49.44%)of variants within the locus was located within the kinase domainⅢ,and several of which were evidently associated with the diversity in SFW response,indicating the critical role of kinase domainⅢin expression of disease *** findings provide valuable information for further functional analysis of SFW resistance genes and marker-assisted resistance breeding in sesame.

关键词： Breeding for resistance Genetic analysis Molecular analysis Tandem kinase domain

Integrated top-down and bottom-up proteomics mass spectrometry for the characterization of endogenous ribosomal protein heterogeneity

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Journal of Pharmaceutical Analysis 2023年第1期13卷 63-72页

作者： Ying Zhang Qinghua Cai Yuxiang Luo Yu Zhang Huilin Li School of Pharmaceutical Sciences Sun Yat-sen UniversityGuangzhou510006China Henan Engineering laboratory for Mammary Bioreactor School of Life SciencesHenan UniversityKaifengHenan475004China the shennong laboratory Zhengzhou450002China Guangdong Key laboratory of Chiral Molecule and Drug Discovery School of Pharmaceutical SciencesSun Yat-sen UniversityGuangzhou510006China

Ribosomes are abundant,large RNA-protein complexes that are the sites of all protein synthesis in *** in ribosomal proteins(RPs),including proteoforms arising from genetic variations,alternative splicing of RNA transcripts,post-translational modifications and alterations of protein expression level,have been linked to a diverse range of diseases,including cancer and *** characterization of ribosomal proteoforms is challenging but important for the discovery of potential disease biomarkers or protein *** the present work,using *** 70S RPs as an example,we first developed a top-down proteomics approach on a Waters Synapt G2 Si mass spectrometry(MS)system,and then applied it to the HeLa 80S *** results were complemented by a bottom-up *** total,50 out of 55 RPs were identified using the top-down *** these,more than 30 RPs were found to have their N-terminal methionine *** modifications such as methylation,acetylation,and hydroxylation were also observed,and the modification sites were identified by bottomup *** a HeLa 80S ribosomal sample,we identified 98 ribosomal proteoforms,among which multiple truncated 80S ribosomal proteoforms were observed,the type of information which is often overlooked by bottom-up *** their relevance to diseases is not yet known,the integration of topdown and bottom-up proteomics approaches paves the way for the discovery of proteoform-specific disease biomarkers or targets.

关键词： Ribosomal proteins Top-down MS Bottom-up MS Proteoforms

A new phase of treasure hunting in plant genebanks

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Molecular Plant 2023年第3期16卷 503-505页

作者： Guangwei Li Zhiyong Wang Yuxuan Meng Zheng Qing Fu Daowen Wang Kunpu Zhang State Key laboratory of Wheat and Maize Crop Science Center for Crop Genome Engineeringand College of AgronomyHenan Agricultural UniversityZhengzhouHenan 450002China the shennong laboratory ZhengzhouHenan 450002China Department of Biological Sciences University of South CarolinaColumbiaSC 29208USA

The United Nations has estimated that the world population will surpass 8 billion on Nov.15,2022,and will continue to rise to 11.2 billion by *** that agricultural resources are limited,it will be a huge challenge to produce sufficient food to feed such a rapidly rising global ***,the ongoing climate changes are adding more pressures on worldwide crop *** cope with these problems,it is both imperative and urgent to develop the crop cultivars with higher yield potential,improved nutritional quality,and better resilience to environmental stresses.

关键词： pass phase adding