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The Crop Journal 2015年第4期3卷 328-334页

作者： Yaoguang Li Dongyun Ma Dexiang Sun Chenyang Wang Jian Zhang Yingxin Xie Tiancai Guo Agronomy College National Engineering Research Center for Wheat Henan Agricultural University the collaborative innovation center of henan food crops Henan Agricultural University Institute of food Science and Technology Henan Agricultural University

The objective of this study was to evaluate the effects of wheat variety, food processing, and milling method on antioxidant properties. Black wheat variety Heibaoshi 1 had the highest total phenolic content(659.8 μg gallic acid equivalents g-1), total flavonoid content(319.3 μg rutin equivalents g-1), and antioxidant activity, whereas light purple wheat variety Shandongzimai 1 had the lowest total flavonoid content(236.2 μg rutin equivalents g-1) and antioxidant activity. Whole wheat flour and partially debranned grain flour had significantly higher total phenolic contents, total flavonoid contents, and antioxidant activity than refined flour(P < 0.05). Compared with flour, total phenolic contents, total flavonoid contents and antioxidant activity decreased in noodles and steamed bread, whereas noodles had slightly higher total phenolic and flavonoid content than steamed bread. Antioxidant activities(by ferric reducing ability of plasma assay) of steamed bread made from whole wheat flour, partially debranned grain flour, and refined flour were 23.5%, 21.1%, and 31.6% lower, respectively, than the corresponding values of flour. These results suggested that black whole wheat flour and partially debranned grain flour are beneficial to human health.

关键词： Total phenolic content Total flavonoid content Milling method Antioxidant activity Colored wheat

Molecular characterization of secaloindoline genes in introduced CIMMYT primary hexaploid triticale

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The Crop Journal 2017年第5期5卷 430-437页

作者： Hongmei Liu Xiaobian Zhou Xiangnan Li Junying Chen Dangqun Cui Feng Chen Agricultural College National Key Laboratory of Wheat and Corn Crop Collaborative Innovation Center of Henan Grain Crops Henan Agricultural University

In order to widen gene germplasm for kernel hardness in triticale, 60 synthetic hexaploid triticales were tested by the single kernel characterization system(SKCS) and secaloindoline alleles were identified by sequencing. Phenot^ing showed that frequencies of soft, mixed and hard genotypes were 43.3%, 48.3%, and 8.4%, respectively. Genotyping identified three known secaloindoline-a alleles and four known secaloindoline-b alleles. Three new Sina-Rl alleles were designated Sina-Rld, Sina-Rle and Sina-Rlf. Compared to Sina-Dlc, Sina-Rld showed four point mutations causing changes in four amino acids, Sina-Rle had one point mutation causing an alanine to glycine substitution, and Sina-Rlf possessed five point mutations but produced the same amino add sequence as Sina-Rld. Two new Sinb-Rl alleles were discovered and designated Sinb-Rle and Sinb-Rl/. Compared to Sinb-Rla, Sinb-Rle possessed a triplet-code insertion and four point mutations causing a cysteine insertion and two amino acid substitutions, and Sinb-Rl/possessed three point mutations causing a cysteine insertion and a change of arginine to *** of hardness index with secaloindoline alleles indicated t±iat SKCS of the Sina-Rld genotype was significantly lower than that of Sina-Rle, and Sinb-Rle was significantly lower than that of the Sinb-Rld genotype. A total of eight allelic combinations of secaloindoline genes were identified; Sma-Rld/Sinb-Rle and Sina-Rle/Sinb-Rld were relatively prevalent in the triticales surveyed. The results provide valuable information for further use of these germplasms in triticale breeding program due to the diverse polymorphism in secaloindoline genes.

关键词： Kernel hardness Allelic variation Secaloindoline genes Hexaploid triticale

Superior grains determined by grain weight are not fully correlated with the flowering order in rice

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Journal of Integrative Agriculture 2015年第5期14卷 847-855页

作者： PENG Ting L Qiang ZHAO Ya-fan SUN Hong-zheng HAN Ying-chun DU Yan-xiu ZHANG Jing LI Jun-zhou WANG Lin-lin ZHAO Quan-zhi collaborative innovation center of henan Grain crops Henan Agricultural University Research center for Rice Engineering in henan Province Henan Agricultural University College of Agronomy Henan University of Science and Technology

Rice panicles are composed of many branches with two types of extreme grains,the superior and the ***,it has been well accepted that earlier flowers result in superior grains and late flowers generate inferior ***,these correlations have never been strictly examined in *** order to determine the accurate relationship between superior and inferior grains and the flowering order,we localized all the seeds in a panicle in four distinct rice species and systematically documented the rice flowering order,flower locations and the final grain weight for their *** results demonstrated that the grain weight is more heavily determined by the position of the seeds than by the flowering *** earlier flowering has a positive correlation with the grain weight in general,grains from flowers blooming on the second day after anthesis generally gained the highest *** suggests earlier flowers may not result in superior ***,we concluded that superior and inferior grains,commonly determined by grain weight,are not fully correlated with the flowering order in *** the order of the grain weight,the superior grains are generally localized at the middle parts of the primary branches,whereas inferior grains were mainly on the last two secondary branches of the lower half part of the *** addition,the weight of inferior grains were affected by spikelet thinning and spraying with exogenous plant growth regulators,indicating that physiological incompetence might be the major reason for the occurrence of the inferior grains.

关键词： flowering grains panicle inferior branches physiological flowers exogenous filling thinning

QTL consistency for agronomic traits across three generations and potential applications in popcorn

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Journal of Integrative Agriculture 2015年第12期14卷 2547-2557页

作者： DONG Yong-bin ZHANG Zhong-wei SHI Qing-ling WANG Qi-lei ZHOU Qiang DENG Fei MA Zhi-yan QIAO Da-he LI Yu-ling College of Agronomy Henan Agricultural University/Collaborative Innovation Center of Henan Grain Crops/National Key Laboratory of Wheat and Maize Crop Science

Favorable agronomic traits are important to improve productivity of popcorn. In this study, a recombinant inbred line（RIL） population consisting of 258 lines was evaluated to identify quantitative trait loci（QTLs） for nine agronomic traits（plant height, ear height, top height（plant height subtracted ear height）, top height/plant height, number of leaves above the top ear, leaf area, stalk diameter, number of tassel branches and the length of tassel） under three environments. Meta-analysis was conducted then to integrate QTLs identified across three generations（RIL, F2：3 and BC2F2） developed from the same crosses. In total, 179 QTLs and 36 meta-QTLs（m QTL） were identified. The percentage of phenotypic variation（R2） explained by any single QTL varied from 3.86 to 28.4%, and 24 QTLs with contributions over 15%. Nine common QTLs located in the same or similar chromosome regions were detected across three generations. Five meta-QTLs were identified including QTLs in three independent studies. Seven important m QTLs were composed of 11–26 QTLs for 4–7 traits, respectively. Only 11 m QTLs were commonly identified in the same or similar chromosome regions across agronomic traits, popping characteristics（popping fold, popping volume and popping rate） and grain yield components（ear weight per plant, grain weight per plant, 100-grain weight, ear length, kernel number per row, ear diameter, row number per ear and kernel ratio） by meta-QTL analysis. In conclusion, we identified a list of QTLs, some of which with much higher contributions to agronomic traits should be valuable for further study in improving both popping characteristics and grain yield components in popcorn.

关键词： popcorn agronomic traits quantitative trait locus(QTL) meta-analysis

Carbon Mineralization and Microbial Attributes in Straw-Amended Soils as Affected by Moisture Levels

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Pedosphere 2014年第2期24卷 167-177页

作者： CHEN Lin ZHANG Jia-Bao ZHAO Bing-Zi XIN Xiu-Li ZHOU Gui-Xiang TAN Jin-Fang ZHAO Jin-Hua State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil ScienceChinese Academy of Sciences University of Chinese Academy of Sciences collaborative innovation center of food crops in henan Henan Agriculture University

An 80-d incubation experiment was conducted to investigate straw decomposition, the priming effect and microbial characteristics in a non-fertilized soil （soil 1） and a long-term organic manure-fertilized soil （soil 2） with and without 13C-labeled maize straw amendment under different moisture levels. The soil 2 showed a markedly higher priming effect, microbial biomass C （Cmic）, and β-glucosidase activity, and more abundant populations of bacteria and fungi than the soil 1. Also, soil CO2 emission, Cmic, /3- glucosidase activity, and bacterial and fungal population sizes were substantially enhanced by straw amendment. In the presence of straw, the amount of straw mineralization and assimilation by microbes in the soil at 55% of water holding capacity （WHC） were significantly higher by 31% and 17%, respectively, compared to those at 25% of WHC. In contrast, β-glucosidase activity and fungal population size were both enhanced as the moisture content decreased. Cmic decreased as straw availability decreased, which was mainly attributed to the reduction of straw-derived Cmic. Amended soils, except the amended soil 2 at 25% of WHC, had a more abundant fungal population as straw availability decreased, indicating that fungal decomposability of added straw was independent of straw availability. Non-metric multidimensional scaling analysis based on fungal denatured gradient gel electrophoresis band patterns showed that shifts in the fungal community structure occurred as water and straw availability varied. The results indirectly suggest that soil fungi are able to adjust their degradation activity to water and straw availability by regulating their community structure.

关键词： bacteria decomposition fungi maize straw organic manure

Test of Small RNA Sequencing Repeatability in Rice

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Rice science 2017年第1期24卷 56-60页

作者： SUN Hong-zheng PENG Ting ZHANG Jing LI Jun-zhou DU Yan-xiu ZHAO Quan-zhi collaborative innovation center of henan Grain crops/Key Laboratory of Rice Biology in henan Province/Agronomy College Henan Agricultural UniversityZhengzhou 450002China

Deep sequencing of small RNAs（sR NA） is widely used in sR NAs studies in plants. In order to investigate the sequencing frequency variation of sR NAs, the same sR NA samples from rice grains were sequenced twice using deep sequencing technique. The sR NAs were classified into three categories, high abundance（〉 100 RPM）, medium abundance（10–100 RPM） and low abundance（1–10 RPM）. According to the repeat sequencing data of the same sample, highly expressed sR NAs（〉 100 RPM） were less subject to random drift, and 95% of the sR NAs Log2 ratio between two samples fell between-0.649 and 0.558. The same trend was observed in mediumly expressed sR NAs（10–100 RPM）, and 95% of the Log2 ratio fell between-0.535 and 0.759. As to lowly expressed sR NAs（1–10 RPM）, 95% of the Log2 ratio varied between-1.009 and 1.011. These results can be used as a theoretical guide to find differentially expressed s RNAs in sR NA studies in plants.

关键词： sequencing Sequencing abundance sequenced varied drift ranged fitted removing RNA

Systematic dissection of disease resistance to southern corn rust by bulked-segregant and transcriptome analysis

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The Crop Journal 2022年第2期10卷 426-435页

作者： Xiaohuan Mu Zhuangzhuang Dai Zhanyong Guo Hui Zhang Jianping Yang Xinke Gan Jiankun Li Zonghua Liu Jihua Tang Mingyue Gou State Key Laboratory of Wheat and Maize Crop Science Collaborative Innovation Center of Henan Grain CropsCollege of AgronomyHenan Agricultural UniversityZhengzhou 450002HenanChina

Southern corn rust(SCR) is a destructive maize disease caused by Puccinia polysora Underw. To investigate the mechanism of SCR resistance in maize, a highly resistant inbred line, L119 A, and a highly susceptible line, Lx9801, were subjected to gene mapping and transcriptome analysis. Bulked-segregant analysis coupled with whole-genome sequencing revealed several quantitative trait loci(QTL) on chromosomes 1, 6, 8, and 10. A set of 25 genes, including two coiled-coil nucleotide-binding site leucine-rich repeat(CC-NBS-LRR) genes, were identified as candidate genes for a major-effect QTL on chromosome 10. To investigate the mechanism of SCR resistance in L119 A, RNA-seq of P. polysorainoculated and non-inoculated plants of L119 A and Lx9801 was performed. Unexpectedly, the number of differentially expressed genes in inoculated versus non-inoculated L119 A plants was about 10 times that of Lx9801, with only 29 common genes identified in both lines, suggesting extensive gene expression changes in the highly resistant but not in the susceptible line. Based on the transcriptome analysis, one of the CC-NBS-LRR candidate genes was confirmed to be upregulated in L119 A relative to Lx9801 independently of P. polysora inoculation. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses indicated that transcription factors, as well as genes involved in defense responses and metabolic processes, were dominantly enriched, with the phenylpropanoid biosynthesis pathway most specifically activated. Consistently, accumulation of phenylpropanoid-derived lignin, especially S lignin, was drastically increased in L119 A after P. polysora inoculation, but remained unchanged in Lx9801, suggesting a critical role of lignin in SCR resistance. A regulatory network of defense activation and metabolic change in SCR-resistant maize upon P. polysora infection is described.

关键词： Maize Southern corn rust BSA-seq RNA-seq Lignin

Comparative QTL analysis of maize seed artificial aging between an immortalized F_2 population and its corresponding RILs

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The Crop Journal 2016年第1期4卷 30-39页

作者： Bin Wang Zhanhui Zhang Zhiyuan Fu Zonghua Liu Yanmin Hu Jihua Tang State Key Laboratory of Wheat and Maize Crop Science/collaborative innovation center of henan Grain crops Henan Agricultural University College of Agronomy Henan University of Science and Technology

Seed aging decreases the quality and vigor of crop seeds,thereby causing substantial agricultural and economic losses in *** identify genetic differences in seed aging between homozygotes and heterozygotes in maize,the seeds of a set of recombinant inbred lines(RILs) and an immortalized F_2(IF_2) population were subjected to artificial aging treatments for 0,2,3,and 4 days under 45℃ and 85%relative humidity and seed vigor was then evaluated in a field *** vigor of all entries tested decreased sharply with longer aging treatment and seed vigor decreased more slowly in heterozygotes than in ***-nine QTL were detected for four measured seed vigor traits in the RIL(28QTL) and IF_2(21 QTL) *** one QTL,qGP5,was detected in both populations,indicating that the genes involved in anti-aging mechanisms differed between inbred lines and *** QTL were identified to be responsible for multiple seed vigor traits simultaneously in the RIL and IF_2 populations under artificial aging *** QTL may include major genes for seed vigor or seed *** qVI4 b and qGE3 a detected in the RIL population coincided with genes ZmLOX1 and ZmPLD1 in the same respective chromosomal *** QTL would be useful for screening for anti-aging genes in maize breeding.

关键词： Maize(Zea mays L.) Seed vigor Artificial aging QTL mapping Seed storage

Receptor-like kinase OsASLRK regulates methylglyoxal response and content in rice

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Journal of Integrative Agriculture 2021年第7期20卷 1731-1742页

作者： LIN Fa-ming LI Shen WANG Ke TIAN Hao-ran GAO Jun-feng DU Chang-qing collaborative innovation center of henan Grain crops/henan Key Laboratory of Rice Biology/College of Agronomy Henan Agricultural UniversityZhengzhou 450002P.R.China

Receptor-like kinases(RLKs) are essential for plant abiotic stress responses. Methylglyoxal(MG) is a cellular metabolite that is often considered to be a stress signal molecule. However, limited information is available about the relationship between RLKs and MG. Here, we addressed the function of a receptor-like kinase, Os ASLRK, in the MG response and content in rice. A typical MG-responsive element(AAAAAAAA) exists in the promoter region of the OsASLRK gene. RTqPCR analysis indicated that the transcript level of OsASLRK was significantly increased by exogenous MG in a time-and dosage-dependent fashion. GUS staining also confirmed that the expression of Os ASLRK in rice root was enhanced by exogenous MG treatment. Genetic analysis suggested that the Osaslrk mutant displays increased sensitivity to MG and it showed higher endogenous MG content under exogenous MG treatments, while OsASLRK-overexpressing rice plants showed the opposite phenotypes. Diaminobenzidine(DAB) staining, scavenging enzyme activities and GSH content assays indicate that OsASLRK regulates MG sensitivity and content via the elevation of antioxidative enzyme activities and alleviation of membrane damage. Therefore, our results provide new evidence illustrating the roles that receptor-like kinase Os ASLRK plays in MG regulation in rice.

关键词： receptor-like kinase(RLK) Os ASLRK methylglyoxal(MG) rice

Influence of water potential and soil type on conventional japonica super rice yield and soil enzyme activities

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Journal of Integrative Agriculture 2017年第5期16卷 1044-1052页

作者： ZHANG Jing WANG Hai-bin LIU Juan CHEN Hao DU Yan-xiu LI Jun-zhou SUN Hong-zheng PENG Ting ZHAO Quan-zhi College of Agronomy Henan Agricultural University/Collaborative Innovation Center of Henan Grain Crops/Henan Key Laboratory of Rice BiologyZhengzhou 450002P.R.China

We carried out a pool culture experiment to determine the optimal water treatment depth in loam and clay soils during the late growth stage of super rice. Three controlled water depth treatments of 0-5, 0-10 and 0-15 cm below the soil surface were established using alternate wetting and drying irrigation, and the soil water potential （0 to -25 kPa） was measured at 5, 10 and 15 cm. A 2-cm water layer was used as the control. We measured soil enzyme activities, root antioxidant enzyme activities, chlorophyll fluorescence parameters, and rice yield. The results showed that the 0-5-cm water depth treatment significantly increased root antioxidant enzyme activities in loam soil compared with the control, whereas soil enzyme activities, chlorophyll fluorescence parameters and yield did not differ from those of the control. The 0-10- and 0-15-cm water depth treatments also increased root antioxidant enzyme activities, whereas soil enzyme activities, chlorophyll fluorescence parameters and yield decreased. In clay soil, the soil enzyme activities, root antioxidant enzyme activities, chlorophyll fluorescence parameters, and yield did not change with the 0-5-cm water treatment, whereas the 0-10- and 0-15-cm water treatments improved these parameters. Therefore,the appropriate depths for soil water during the late growth period of rice with a 0 to -25 kPa water potential were 5 cm in loam and 15 cm in clay soil.

关键词： rice yield components soil type soil enzyme activity antioxidant enzyme activity chlorophyll fluorescence parameters water potential