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Chemical Research in Chinese Universities 2019年第6期35卷 967-971页

作者： WANG Shuxin CAO Jian CHENG Yuxiao LU Chenhong School of Chemistry^and Chemical Engineering Shanghai University of Engineering ScienceShanghai 201620P.R.China technical center for Industrial Product and Raw Material inspection and Testing Shanghai CustomsShanghai 200135P.R.China

A highly sensitive fluorescent“turn-on”probe NapP-deap based on naphthalimide derivative was developed that bound Hg^2+ ions rapidly in the N-2-hydroxyethylpiperazine-N-ethane-sulphonic acid(HEPES)buffer solution via photo-induced electron transfer(PET)being inhibited *** titration experiment displayed that the emission intensity of NapP-deap at 540 nm was almost linearly increased by about *** Job’s plot showed a stoichiometry factor of 1:1 of the ligand-to-metal *** detection limit of fluorescent probe was calculated to be 6.2×10^?9 mol/L.^1H NMR studies could confirm that one Hg^2+ ion was bound by the N atoms(a,b)of piperazine or the N atom(c)of *** fluorescent probe could be used for the detection of Hg^2+ ions in living cells.

关键词： Fluorescent “turn-on” Hg^2+ HEPES buffer solution Living cell PET mechanism

Single-cell omics: experimental workflow, data analyses and applications

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Science China Life Sciences 2025年第1期68卷 5-102页

作者： Fengying Sun Haoyan Li Dongqing Sun Shaliu Fu Lei Gu Xin Shao Qinqin Wang Xin Dong Bin Duan Feiyang Xing Jun Wu Minmin Xiao Fangqing Zhao Jing-Dong J.Han Qi Liu Xiaohui Fan Chen Li Chenfei Wang Tieliu Shi Department of Clinical Laboratory the Affiliated Wuhu Hospital of East China Normal University (The Second People's Hospital of Wuhu City) Pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang University Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration (Tongji University) Ministry of EducationOrthopaedic DepartmentTongji HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Frontier Science center for Stem Cells School of Life Sciences and TechnologyTongji University Translational Medical center for Stem Cell Therapy and Institute for Regenerative Medicine Shanghai East HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Research Institute of Intelligent Computing Zhejiang Lab Shanghai Research Institute for Intelligent Autonomous Systems center for Single-cell Omics School of Public HealthShanghai Jiao Tong University School of Medicine National Key Laboratory of Chinese Medicine Modernization Innovation Center of Yangtze River DeltaZhejiang University center for Bioinformatics and Computational Biology Shanghai Key Laboratory of Regulatory Biologythe Institute of Biomedical Sciences and School of Life SciencesEast China Normal University Beijing Institutes of Life Science Chinese Academy of Sciences Peking-Tsinghua center for Life Sciences Academy for Advanced Interdisciplinary StudiesCenter for Quantitative Biology (CQB)Peking University Zhejiang Key Laboratory of Precision Diagnosis and Therapy for Major Gynecological Diseases Women's HospitalZhejiang University School of Medicine Key Laboratory of Advanced Theory and Application in Statistics and Data Science-MOE School of StatisticsEast China Normal University

Cells are the fundamental units of biological systems and exhibit unique development trajectories and molecular features. Our exploration of how the genomes orchestrate the formation and maintenance of each cell, and control the cellular phenotypes of various organismsis, is both captivating and intricate. Since the inception of the first single-cell RNA technology, technologies related to single-cell sequencing have experienced rapid advancements in recent years. These technologies have expanded horizontally to include single-cell genome, epigenome,proteome, and metabolome, while vertically, they have progressed to integrate multiple omics data and incorporate additional information such as spatial sc RNA-seq and CRISPR screening. Single-cell omics represent a groundbreaking advancement in the biomedical field,offering profound insights into the understanding of complex diseases, including cancers. Here, we comprehensively summarize recent advances in single-cell omics technologies, with a specific focus on the methodology section. This overview aims to guide researchers in selecting appropriate methods for single-cell sequencing and related data analysis.

关键词： single-cell sequencing genome epigenome proteomics metabolomics multimodal spatial transcriptomics CRISPR screening

Synthesis of Water-dispersed Polyurethane Adhesive and Its Application in Pigment Printing

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Journal of Donghua University(English Edition) 2004年第4期21卷 8-11页

作者：林苗杨勇 College of Chemistry and Chemical Engineering Donghua University Shanghai 200051associate professor inspection center of Industrial Product & Raw Materials Shanghai Entry-Exit Inspection & Quarantine Bureau Shanghai200135

Water-dispersed polyurethane (PU) adhesive is a novel and highly efficient adhesive with broad application potential. In this study, the key parameters affecting the synthesis and application of this adhesive were examined, and optimal conditions were identified. The water-dispersed PU adhesive was successfully synthesized, and applied in the fastness test of pigment printing on cotton fabric. The data demonstrated that all the fastnesses of PU adhesive were better than that of the conventional PA one.

关键词： water-dispersed polyurethane adhesive pre-polymerization blocking reaction fastness.

A tunable lighting system integrated by inorganic and transparent organic light-emitting diodes

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Optoelectronics Letters 2014年第3期10卷 198-201页

作者：张晶晶张涛金亚方刘石神袁士东崔钊张立王伟辉 Shanghai Institute of technical Physics Chinese Academy of Sciences National center for Quality inspection & Supervision of LED Product Changzhou Institute of Opto-Electronic Technology School of Electronic and Optical Engineering Nanjing University of Science and Technology

A tunable surface-emitting integrated lighting system is constructed using a combination of inorganic light-emitting diodes(LEDs) and transparent organic LEDs(OLEDs).An RB two-color LED is used to supply red and blue light emission,and a green organic LED is used to supply green light *** of the LED and OLED are tuned to produce a white color,showing different Commission Internationale d'Eclairage(CIE) chromaticity coordinates and correlated color temperatures with a wide adjustable *** an integration can compensate for the lack of the LED's luminance uniformity and the transparent OLED's luminance intensity.

关键词：有机发光二极管照明系统集成可调无机国际照明委员会 OLED 亮度均匀性

A Benzodithiophene-Based Fluorescence Probe for Rapid Detection of Fluoride Ion

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Chinese Journal of Chemistry 2016年第8期34卷 809-813页

作者： Wenbin Tan Taohua Leng Guoqiao Lai Zhifang Li JiefeiWu Yongjia Shen Chengyun Wang Key Laboratory for Advanced Materials and Institute of Fine Chemicals East China University of Science and Technology Shanghai 200237 China National Food Quality Supervision and inspection center (Shanghai)/Shanghai Institute of Quality inspection and technical Research Shanghai 200233 China Key Laboratory of Organosilicon Chemistry and Material Technology of Ministry of Education Hangzhou Nor- mal University Hangzhou Zhejiang 310012 China

A novel and simple fluorescence probe was synthesized from benzo[1,2-b:4,5-b′]dithiophene (BDT) and trimethylsilylethyne via Sonogashira reaction, and showed highly selective and sensitive fluorescence decreasing response towards F−. The probe molecule turned to a weakly fluorescent terminal alkyne moiety because its trimethylsilyl (TMS) group was cleaved by fluoride, which was proved by 1H NMR titration. Whereas no distinct fluorescent changes were observed with the addition of other anions, such as Cl−, Br−, I−, AcO− and H2PO4−. Upon the addition of F−, the maximum fluorescence emission wavelength shifted from 460 nm to 450 nm with a decrease of fluorescence intensity by 40% within 20 s. Moreover, the detection limit towards F− was calculated to be as low as 73.5 nmol/L.

关键词： fluorescent probe benzodithiophene fluoride anion trimethylsilylethynyl group

Isolation and phylogenetic study of Rift Valley fever virus from the first imported case to China

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Virologica Sinica 2017年第3期32卷 253-256页

作者： Yongxia Shi Kui Zheng Xiaobo Li Liqiang Li Shufen Li Jinmin Ma Jun Dai Jingkai Ji Shuai Yuan Haorong Lu Jiandong Li Fangfang Sun Xun Xu Jicheng Huang inspection and Quarantine Technology center Guangdong Entry-Exit Inspection and Quarantine Bureau BGI-Shenzhen China National Gene Bank-Shenzhen BGI-Shenzhen Department of Biology University of Copenhagen BGI Education center University of Chinese Academy of Sciences

Dear Editor,Rift Valley fever(RVF)is an anthropozoonosis caused by Rift Valley fever virus(RVFV).RVFV belongs to the Phlebovirus genus in the family Bunyaviridae,which is circulating among *** infection with RVFV is g... 详细信息

关键词：裂谷热病毒人畜共患传染病系统进口中国反刍动物人类感染并发症

Suitability of open-field autorefractors as pupillometers and instrument design effects

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International Journal of Ophthalmology(English edition) 2017年第4期10卷 567-572页

作者： Carles Otero Mikel Aldaba Oriol Ferrer Andrea Gascón Juan C Ondategui-Parra Jaume Pujol Davalor Research center Technical University of Catalonia

AIM：To determine the agreement and repeatability of the pupil measurement obtained with VIP-200（Neuroptics）,Power Ref II（Plusoptix）,WAM-5500（Grand Seiko） and study the effects of instrument design on ***：Forty patients were measured twice in low,mid and high *** was analyzed with the within-subject standard deviation（Sw） and paired *** was studied with Bland-Altman plots and repeated measures *** design analysis consisted on measuring pupil size with Power Ref II simulating monocular and binocular conditions as well as with proximity cues and without proximity ***：The mean difference（±standard deviation） between test-retest for low,mid and high mesopic conditions were,respectively：-0.09（±0.16）,-0.05（±0.18） and-0.08（±0.23） mm for Neuroptics,-0.05（±0.17）,-0.12（±0.23） and-0.17（±0.34） mm for WAM-5500,-0.04（±0.27）,-0.13（±0.37） and-0.11（±0.28） mm for Power Ref *** agreement with Neuroptics,the mean difference for low,mid and high mesopic conditions were,respectively：-0.48（±0.35）,-0.83（±0.52） and-0.38（±0.56） mm for WAM-5500,-0.28（±0.56）,-0.70（±0.55） and-0.61（±0.54） mm for Power Ref *** mean difference of binocular minus monocular pupil measurements was：-0.83（±0.87） mm;and with proximity cues minus without proximity cues was：-0.30（±0.77） ***：All the instruments show similar *** all illumination conditions,agreement of Neuroptics with WAM-5500 and Power Ref II is not good enough,which can be partially induced due to their open field design.

关键词： pupil repeatability agreement open-field

Integrated development planning in mountainous areas-the case of Konitsa,Greece

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Journal of Mountain Science 2016年第1期13卷 169-182页

作者： Vaios KOTSIOS Metsovion Interdisciplinary Research center National Technical University of Athens

In the research literature,we have identified a lack of common understanding of the "development" *** meaning of development has changed over the last decades from referencing simple economic growth to embracing social and environmental *** aim of this paper is to highlight the Worth-living Integrated Development theory(WID) in the context of postgraduate education and *** particular,through the description of three research projects undertaken within the interdisciplinary postgraduate programme "Environment and Development of Mountainous Areas" of the National technical University of Athens,the methodology of WID applied to the Greek mountain area of Konitsa is *** the first step of the methodology,the recent development strategies enacted by the municipality of Konitsa were compared with the principles and the values of *** was done to document the necessity of an integrated development *** the second step,the investigating parameters and indicators were selected and then bibliographically and empirically researched following extensive discussions within the interdisciplinary *** the third step,the data were analyzed to highlight the problems,the objective and the "potential" development opportunities,as well as the objective constraints on the Integrated Development of the study *** the fourth step,a combination of bottom-up and top-down approaches was followed by interdisciplinary workgroups for the formulation and documentation of alternative scenarios for Integrated *** the fifth step,a set of criteria wasdescribed to allow residents to evaluate the proposed development actions and choose the optimal development policies for the Municipality of ***,a set of principles and values was clarified to help residents speak out and reject actions or policies that might lower their living *** results of each methodological step and the integrated development approach for t

关键词： highlight necessity scenarios clarified Athens speak document formulation disciplines discussions

Tracing drug release process with dual-modal hyperbranched polymer-gold nanoparticle complexes

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Science China Chemistry 2016年第12期59卷 1600-1608页

作者： Yuanyuan Zhuang Dali Wang Chunhui Yin Hongping Deng Mo Sun Lin He Yue Su Xinyuan Zhu Shanghai Key Lab of Electrical Insulation and Thermal Aging School of Chemistry and Chemical Engineering Shanghai Jiao Tong University Shanghai 200240 China Shanghai Institute of Quality inspection and technical Research Shanghai 201114 China Instrumental Analysis center Shanghai Jiao Tong University Shanghai 200240 China

Dual-modal surface enhanced Raman spectrum(SERS)-fluorescence polymer/metal hybrid complexes have been prepared for tracing drug release process in tumor cells. Firstly, the hyperbranched poly((S-(4-vinyl) benzyl S′-propyltrithiocarbonate)-co-(poly(ethylene glycol) methacrylate))(HPVBEG) was synthesized via the combination of reversible addition-fragmentation chain-transfer(RAFT) polymerization and self-condensing vinyl polymerization(SCVP). Subsequently, the anticancer drug doxorubicin(DOX) was linked to HPVBEG via pH sensitive Schiff base bonds to form HPVBEG-g-DOX *** aminolysis reaction, HPVBEG-g-DOX was coordinated with gold nanoparticles(GNP), resulting in the formation of HPVBEG-g-DOX/GNP complexes. In neutral condition, the HPVBEG-g-DOX/GNP complexes were stable, and DOX was bound to the surface of GNPs. Therefore, the SERS of DOX could be observed, while the fluorescence of DOX was quenched by GNPs. Under an acidic environment, DOX was released from the surface of GNPs with breakage of Schiff base ***, the SERS signal of DOX was gradually reduced. Correspondingly, the fluorescence signal of DOX was *** dual-modal SERS-fluorescence technique, the DOX delivery and release process was traced in tumor cells. Moreover,the viability of MCF-7 cells incubated with HPVBEG-g-DOX/GNP complexes was investigated by Cell Counting Kit-8(CCK-8) assay. The experimental results showed that HPVBEG-g-DOX/GNP complexes had similar proliferation inhibition effect compared with free DOX. Definitely, the dual-modal SERS-fluorescence complexes for tracing drug delivery and release will have promising prospects on tumor diagnosis and therapy.

关键词： hyperbranched polymer nanoparticle SERS fluorescence imaging drug carrier

Investigation of the segregation of a binary particle mixture in a square circulating fluidized bed with air staging

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Particuology 2019年第6期17卷 70-76页

作者： Mingkun Du Shuai Wang Guangdong Institute of Special Equipment inspection and Research Zhuhai Inspection InstituteZhuhai 519002China School of Energy Science and Engineering Harbin Institute of TechnologyHarbin 150001China

To further understand the segregation characteristics of a binary particle mixture in a riser,the flow behaviors of particles with different sizes were experimentally and numerically investigated in a cold square circulating fluidized *** impact of the gas velocity,solid circulation rate,initial coarse particle fraction,and air staging on the coarse particle fraction and axial pressure drop were *** experimental results show that air staging can significantly promote the pressure drop of a binary mixture at the bottom of the bed compared with that of monosized ***,the segregation of the binary mixture at the bottom is *** was also found that there was clear radial segregation owing to a higher coarse particle fraction near the wall compared with the bed's center and its corner.

关键词： Circulating fluidized bed Binary mixture Segregation Air staging