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Enhancement of porcine in vitro embryonic development through luteolin‑mediated activation of the Nrf2/Keap1 signaling pathway

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Journal of animal Science and Biotechnology 2024年第2期15卷 600-613页

作者： Se-Been Jeon Pil-Soo Jeong Min Ju Kim Hyo-Gu Kang Bong-Seok Song Sun-Uk Kim Seong-Keun Cho Bo-Woong Sim Futuristic animal Resource&research Center(FARRC) Korea Research Institute of Bioscience and Biotechnology(KRIBB)Cheongju 28116Republic of Korea department of animal Science College of Natural Resources&Life SciencePusan National UniversityMiryang 50463Republic of Korea department of animal Science and Biotechnology College of Agriculture and Life ScienceChungnam National UniversityDaejeon 34134Republic of Korea department of Functional Genomics University of Science and TechnologyDaejeon 34113Republic of Korea department of animal Science Life and Industry Convergence Research Institute(RICRI)College of Natural Resources&Life SciencePusan National UniversityMiryang 50463Republic of Korea

Background Oxidative stress,caused by an imbalance in the production and elimination of intracellular reactive oxygen species(ROS),has been recognized for its detrimental effects on mammalian embryonic development.Luteolin(Lut)has been documented for its protective effects against oxidative stress in various studies.However,its specific role in embryonic development remains unexplored.This study aims to investigate the influence of Lut on porcine embryonic development and to elucidate the underlying mechanism.Results After undergoing parthenogenetic activation(PA)or in vitro fertilization,embryos supplemented with 0.5μmol/L Lut displayed a significant enhancement in cleavage and blastocyst formation rates,with an increase in total cell numbers and a decrease in the apoptosis rate compared to the control.Measurements on D2 and D6 revealed that embryos with Lut supplementation had lower ROS levels and higher glutathione levels compared to the control.Moreover,Lut supplementation significantly augmented mitochondrial content and membrane potential.Intriguingly,activation of the Nrf2/Keap1 signaling pathway was observed in embryos supplemented with Lut,leading to the upregulation of antioxidant-related gene transcription levels.To further validate the relationship between the Nrf2/Keap1 signaling pathway and effects of Lut in porcine embryonic development,we cultured PA embryos in a medium supplemented with brusatol,with or without the inclusion of Lut.The positive effects of Lut on developmental competence were negated by brusatol treatment.Conclusions Our findings indicate that Lut-mediated activation of the Nrf2/Keap1 signaling pathway contributes to the enhanced production of porcine embryos with high developmental competence,and offers insight into the mechanisms regulating early embryonic development.

关键词： Luteolin Mitochondrial function Nrf2/Keap1 signaling pathway Oxidative stress Porcine embryo development

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Application of a simplified ADM1 for full-scale anaerobic co-digestion of cattle slurry and grass silage:assessment of input variability

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Frontiers of Environmental Science & Engineering 2024年第4期18卷 125-139页

作者： Sofia Tisocco Sören Weinrich Gary Lyons Michael Wills Xinmin Zhan Paul Crosson Civil Engineering College of Science and EngineeringUniversity of GalwayGalwayH91 TK33Ireland teagasc animal and bioscience research department Animal and Grassland Research and Innovation CentreDunsanyC15 PW93Ireland Biochemical Conversion department Deutsches Biomasseforschungszentrum gemeinnützige GmbHLeipzig 04347Germany Faculty of Energy·Building Services·Environmental Engineering Münster University of Applied SciencesSteinfurt 48565Germany Agri-Food and biosciences Institute HillsboroughBT266DRUK Ryan Institute University of GalwayGalwayH91 TK33Ireland MaREI research Centre for Energy Climate and MarineRyan InstituteUniversity of GalwayGalwayH91 TK33Ireland

Mathematical modeling of anaerobic digestion is a powerful tool to predict gas yields and optimize the process.The Anaerobic Digestion Model No.1(ADM1)is a widely implemented model for this purpose.However,modeling full-scale biogas plants is challenging due to the extensive substrate and parameter characterization required.This study describes the modification of the ADM1 through a simplification of individual process phases,characteristic components and required parameters.Consequently,the ability of the simplified model to simulate the co-digestion of grass silage and cattle slurry was evaluated using data from a full-scale biogas plant.The impacts of substrate composition(crude carbohydrate,protein and lipid concentration)and variability of carbohydrate degradability on simulation results were assessed to identify the most influential parameters.Results indicated that the simplified version was able to depict biogas and biomethane production with average model efficiencies,according to the Nash-Sutcliffe efficiency(NSE)coefficient,of 0.70 and 0.67,respectively,and was comparable to the original ADM1(average model efficiencies of 0.71 and 0.63,respectively).The variability of crude carbohydrate,protein and lipid concentration did not significantly impact biogas and biomethane output for the data sets explored.In contrast,carbohydrate degradability seemed to explain much more of the variability in the biogas and methane production.Thus,the application of simplified models provides a reliable basis for the process simulation and optimization of full-scale agricultural biogas plants.

关键词： ADM1 Agricultural feedstocks Biogas technology Input variability Parameter estimation

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Exploring the modulatory role of bovine lactoferrin on the microbiome and the immune response in healthy and Shiga toxin‑producing E.coli challenged weaned piglets

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Journal of animal Science and Biotechnology 2024年第3期15卷 1278-1291页

作者： Matthias Dierick Ruben Ongena Daisy Vanrompay Bert Devriendt Eric Cox Laboratory of Immunology Department of Translational PhysiologyInfectiology and Public HealthFaculty of Veterinary MedicineGhent UniversitySalisburylaan 1339820 MerelbekeBelgium Laboratory for Immunology and animal Biotechnology Faculty of Bioscience EngineeringGhent UniversityCoupure links 6539000 GhentBelgium

Background Post-weaned piglets suffer from F18+Escherichia coli(E.coli)infections resulting in post-weaning diar-rhoea or oedema disease.Frequently used management strategies,including colistin and zinc oxide,have contrib-uted to the emergence and spread of antimicrobial resistance.Novel antimicrobials capable of directly interacting with pathogens and modulating the host immune responses are being investigated.Lactoferrin has shown promising results against porcine enterotoxigenic E.coli strains,both in vitro and in vivo.Results We investigated the influence of bovine lactoferrin(bLF)on the microbiome of healthy and infected weaned piglets.Additionally,we assessed whether bLF influenced the immune responses upon Shiga toxin-producing E.coli(STEC)infection.Therefore,2 in vivo trials were conducted:a microbiome trial and a challenge infection trial,using an F18+STEC strain.BLF did not affect theα-andβ-diversity.However,bLF groups showed a higher relative abundance(RA)for the Actinobacteria phylum and the Bifidobacterium genus in the ileal mucosa.When analysing the immune response upon infection,the STEC group exhibited a significant increase in F18-specific IgG serum levels,whereas this response was absent in the bLF group.Conclusion Taken together,the oral administration of bLF did not have a notable impact on theα-andβ-diversity of the gut microbiome in weaned piglets.Nevertheless,it did increase the RA of the Actinobacteria phylum and Bifi-dobacterium genus,which have previously been shown to play an important role in maintaining gut homeostasis.Furthermore,bLF administration during STEC infection resulted in the absence of F18-specific serum IgG responses.

关键词： E.coli Immune modulation Lactoferrin Microbiome

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Localization of urea transporter B in the developing bovine rumen

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animal Nutrition 2022年第3期10卷 216-222页

作者： Chongliang Zhong Tamsin Lyons Orla Heussaff Evelyn Doyle Eoin O'Hara Sinead M.Waters David Kenny Gavin S.Stewart School of Biology and Environmental Science University College DublinBelfieldDublin 4Ireland animal and bioscience research department Animal and Grassland Research and Innovation CentreTeagascGrangeCounty MeathIreland department of Agriculture Foodand Nutritional SciencesUniversity of AlbertaEdmontonAlbertaCanada

Urea nitrogen secreted from blood to rumen is a crucial factor shaping the symbiotic relationship between host ruminants and their microbial populations.Passage of urea across rumen epithelia is facilitated by urea transporter B(UT-B),but the long-term regulation of these proteins remains unclear.As ruminal function develops over a period of months,the developing rumen is an excellent model with which to investigate this regulation.Using rumen epithelium samples of calves from birth to 96 d of age,this study performed immunolocalization studies to localize and semi-quantify UT-B protein development.As expected,preliminary experiments confirmed that ruminal monocarboxylate transporter 1(MCT1)short chain fatty acid transporter protein abundance increased with age(P<0.01,n=4).Further investigation revealed that ruminal UT-B was present in the first few weeks of life and initially detected in the basolateral membrane of stratum basale cells.Over the next 2 months,UT-B staining spread to other epithelial layers and semi-quantification indicated that UT-B abundance significantly increased with age(P<0.01,n=4 or 6).These changes were in line with the development of rumen function after the advent of solid feed intake and weaning,exhibiting a similar pattern to both MCT1 transporters and papillae growth.This study therefore confirmed age-dependent changes of in situ ruminal UT-B protein,adding to our understanding of the long-term regulation of ruminal urea transporters.

关键词： UT-B Immunolocalization Rumen Bovine Development

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Alleviatory effect of isoquercetin on benign prostatic hyperplasia via IGF-1/PI3K/Akt/mTOR pathway

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Food Science and Human Wellness 2024年第3期13卷 1698-1710页

作者： Young-Jin Choi Meiqi Fan Nishala Erandi Wedamulla Yujiao Tang Eun-Kyung Kim department of Food Science and Nutrition Dong-A UniversityBusan 49315Republic of Korea Center for Silver-targeted Biomaterials Brain Busan 21 Plus programDong-A UniversityBusan 49315Republic of Korea department of Health Sciences the Graduate School of Dong-A UniversityBusan 49315Republic of Korea Division of Food bioscience College of Biomedical and Health SciencesKonkuk UniversityChungju 27478Republic of Korea department of Export Agriculture Faculty of Animal Science and Export AgricultureUva Wellassa UniversityBadulla 90000Sri Lanka School of Bio-Science and Food Engineering Changchun University of Science and TechnologyChangchun 130600China Nutritional Education Major Graduate School of EducationDong-A UniversityBusan 49315Republic of Korea Nutrinomics Lab.Co. Ltd.Busan 49315Republic of Korea

We evaluated the effect of isoquercetin(quercetin-O-3-glucoside-quercetin,IQ)as a functional component of Abeliophyllum disistichum Nakai ethanol extract(ADLE)on prostate cell proliferation and apoptosis and its effects on the IGF-1/PI3K/Akt/mTOR pathway in benign prostatic hyperplasia(BPH).Metabolites in ADLE were analyzed using UHPLC-qTOF-MS and HPLC.IQ was orally administered(1 or 10 mg/kg)to a testosterone propionate-induced BPH rat model,and its effects on the prostate weight were evaluated.The effect of IQ on androgen receptor(AR)signaling was analyzed in LNCaP cells.Whether IGF-1 and IQ affect the IGF-1/PI3K/Akt/mTOR pathway in BPH-1 cells was also examined.The metabolites in ADLE were identified and quantified,which confirmed that ADLE contained abundant IQ(20.88 mg/g).IQ significantly reduced the prostate size in a concentration-dependent manner in a BPH rat model,and significantly decreased the expression of AR signaling factors in the rat prostate tissue and LNCaP cells in a concentration-dependent manner.IQ also inhibited the PI3K/AKT/mTOR pathway activated by IGF-1 treatment in BPH-1 cells.In BPH-1 cells,IQ led to G0/G1 arrest and suppressed the expression of proliferation factors while inducing apoptosis.Thus,IQ shows potential for use as a pharmaceutical and nutraceutical for BPH.

关键词： Isoquercetin Benign prostatic hyperplasia Androgen receptor signaling PI3K/Akt/mtor pathway

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Effect of genotype on duodenal expression of nutrient transporter genes in dairy cows

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Journal of animal Science and Biotechnology 2014年第2期5卷 145-153页

作者： Sinéad M Waters Kate Keogh Frank Buckley David A Kenny teagasc animal and bioscience research department Animal and Grassland Research and Innovation CentreGrangeDunsanyCo.MeathIreland teagasc animal and bioscience research department Animal and Grassland Research and Innovation Centre MooreparkFermoyCo CorkIreland

Background： Studies have shown clear differences between dairy breeds in their feed intake and production efficiencies. The duodenum is critical in the coordination of digestion and absorption of nutrients. This study examined gene transcript abundance of important classes of nutrient transporters in the duodenum of non lactating dairy cows of different feed efficiency potential, namely Holstein-Friesian （HF）, Jersey （JE） and their F1 hybrid. Duodenal epithelial tissue was collected at slaughter and stored at -80℃. Total RNA was extracted from tissue and reverse transcribed to generate cDNA. Gene expression of the following transporters, namely nucleoside amino acid; sugar; mineral; and lipid transporters was measured using quantitative real-time RT-PCR. Data were statistically analysed using mixed models ANOVA in SAS. Orthogonal contrasts were used to test for potential heterotic effects and spearman correlation coefficients calculated to determine potential associations amongst gen, expression values and production efficiency variables. Results： While there were no direct effects of genotype on expression values for any of the genes examined, there was evidence for a heterotic effect （P 〈 0.05） on ABCGS, in the form of increased expression in the F1 genotype compared to either of the two parent breeds. Additionally, a tendency for increased expression of the amino acid transporters, SLC3A1 （P= 0.072）, SLC3A2 （P= 0.081） and SLC6A 14 （P= 0.072） was also evident in the F1 genotype. A negative （P 〈 0.05） association was identified between the expression of the glucose transporter gene SLCSA1 and total lactational milk solids yield, corrected for body weight. Positive correlations （P 〈 0.05） were also observed between the expression values of genes involved in common transporter roles. Conclusion： This study suggests that differences in the expression of sterol and amino acid transporters in the duodenum could contribute towards the documented differences in feed efficiency between HF, JE and their F1 hybrid. Furthe

关键词： Bovine Duodenum Gene expression Nutrient transporters

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Residual feed intake phenotype and gender affect the expression of key genes of the lipogenesis pathway in subcutaneous adipose tissue of beef cattle

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Journal of animal Science and Biotechnology 2019年第1期10卷 208-217页

作者： Clare McKenna Richard K.Porter Kate A.Keogh Sinead M.Waters Mark McGee David A.Kenny animal and bioscience research department Teagasc Grange School of Biochemistry & Immunology Trinity College Dublin

Background: Feed accounts for up to 75% of costs in beef production systems,thus any improvement in feed efficiency(FE) will benefit the profitability of this enterprise.Residual feed intake(RFI) is a measure of FE that is independent of level of production.Adipose tissue(AT) is a major endocrine organ and the primary metabolic energy reservoir.It modulates a variety of processes related to FE such as lipid metabolism and glucose homeostasis and thus measures of inter-animal variation in adiposity are frequently included in the calculation of the RFI index.The aim of this study was to determine the effect of phenotypic RFI status and gender on the expression of key candidate genes related to processes involved in energy metabolism within AT.Dry matter intake(DMI) and average daily gain(ADG) were measured over a period of 70 d for 52 purebred Simmental heifers(n = 24) and bulls(n = 28) with an initial BW±SD of 372±39.6 kg and 387±50.6 kg,respectively.Residual feed intake was calculated and animals were ranked within gender by RFI into high(inefficient; n = 9 heifers and n = 8 bulls)and low(efficient; n = 9 heifers and n = 8 bulls) groups.Results: Average daily gain ±SD and daily DMI ±SD for heifers and bul s were 1.2±0.4 kg and 9.1±0.5 kg,and 1.8±0.3 kg and 9.5±1 kg respectively.High RFI heifers and bulls consumed 10% and 15% more(P animals across gender.Conclusion: The findings of this study indicate that low RFI cattle exhibit upregulation of the molecular mechanisms governing glucose metabolism in adipose tissue,in particular,glucose clearance.The decreased

关键词： Adipose Cattle RFI SLC2A4

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Stress and immunological response of heifers divergently ranked for residual feed intake following an adrenocorticotropic hormone challenge

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Journal of animal Science and Biotechnology 2018年第1期9卷 111-118页

作者： A.K.Kelly P.Lawrence B.Earley D.A.Kenny M.McGee School of Agriculture and Food Science College of Health and Agricultural SciencesUniversity College Dublin Livestock Systems research department teagasc Animal&Grassland Research and Innovation CentreGrangeDunsanyCo. animal and bioscience research department Animal&Grassland Research and Innovation CentreTeagascDunsanyCo.

Background： When an animal is exposed to a stressor, metabolic rate, energy consumption and utilisation increase primarily through activation of the hypothalamic-pituitary-adrenal（HPA） axis. Changes to partitioning of energy by an animal are likely to influence the efficiency with which it is utilised. Therefore, this study aimed to determine the physiological stress response to an exogenous adrenocorticotropic hormone（ACTH） challenge in beef heifers divergently ranked on phenotypic residual feed intake（RFI）.Results： Data were collected on 34 Simmental weaning beef heifers the progeny of a well characterized and divergently bred RFI suckler beef herd. Residual feed intake was determined on each animal during the postweaning stage over a 91-day feed intake measurement period during which they were individually offered adlibitum grass silage and 2 kg of concentrate per head once daily. The 12 highest [0.34 kg DM/d] and 12 lowest[-0.48 kg DM/d] ranking animals on RFI were selected for use in this study. For the physiological stress challenge heifers（mean age 605 ± 13 d; mean BW 518 ± 31.4 kg） were fitted aseptically with indwelling jugular catheters to facilitate intensive blood collection. The response of the adrenal cortex to a standardised dose of ACTH（1.98 IU/kg metabolic BW^（0.75）） was examined. Serial blood samples were analysed for plasma cortisol, ACTH and haematology variables. Heifers differing in RFI did not differ（P = 0.59） in ACTH concentrations. Concentration of ACTH peaked（P 〈 0.001） in both RFI groups at 20 min post-ACTH administration, following which concentration declined to baseline levels by 150 min. Similarly, cortisol systemic profile peaked at 60 min and concentrations remained continuously elevated for 150 min. A RFI × time interaction was detected for cortisol concentrations（P = 0.06） with high RFI heifers had a greater cortisol response than Low RFI from 40 min to 150 min relative to ACTH administration. Cortisol response was positively associated with RFI status（r = 0.32; P 〈 0.01）. No ef

关键词： Beef cattle Cortisol Feed efficiency Residual feed intake Stress response

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A near infrared spectroscopy calibration for the prediction of fresh grass quality on Irish pastures

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Information Processing in Agriculture 2022年第2期9卷 243-253页

作者： Darren J.Murphy Bernadette O’Brien Michael O’Donovan Tomas Condon Michael D.Murphy teagasc Animal&Grassland Research and Innovation CentreMooreparkFermoyCo.CorkIreland department of Process Energy and Transport EngineeringMunster Technological UniversityCorkIreland

The aim of this study was to develop near infrared spectroscopy(NIRS)calibrations to predict quality parameters,dry matter(DM,g kg1)and crude protein(CP,g kg1 DM),in fresh un-dried grass.Knowledge of these parameters would enable more precise allocation of quality herbage to grazing livestock.Perennial ryegrass samples(n=1615)were collected over the 2017 and 2018 grazing seasons at teagasc Moorepark to develop a NIRS calibration dataset.Additional samples were collected for an independent validation dataset(n=197)during the 2019 grazing season.Samples were scanned using a FOSS 6500 spectrometer at 2 nm intervals in the range of 1100~2500 nm and absorption was recorded as log 1/Reflectance.Reference wet chemistry analysis was carried out for both parameters and the resultant data were calibrated against spectral data by means of modified partial least squares regression.A range of mathematical spectral treatments were examined for each calibration,which were ranked in order of standard error of prediction(SEP)and ratio of percent deviation(RPD).Best performing calibrations achieved high predictive precision for DM(R2=0.86 SEP=9.46 g kg1,RPD=2.60)and moderate precision for CP(R2=0.84 SEP=20.38 g kg1 DM,RPD=2.37).These calibrations will aid the optimisation of grassland management and the development of precision agricultural technologies.

关键词： Near infrared spectroscopy Fresh grass analysis Grassland management Grass quality Precision agriculture

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Structural and Functional Annotation of Hypothetical Protein of Fusobacterium nucleatum Strain MJR7757B: An in Silico Approach

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Computational Molecular bioscience 2024年第1期14卷 17-33页

作者： Md. Isrfil Hossen Fouzia Mostafa Nusrat Jahan Jannatul Ferdaus Amgad Albahi Sayed Mashequl Bari College of Food Science and Technology Huazhong Agricultural University Wuhan China department of Crop Science and Technology Rajshahi University Rajshahi Bangladesh Abdul Malek Ukil Medical College Noakhali Bangladesh department of Medicine IBN Sina Medical College Dhaka Bangladesh National Food research Centre Khartoum Sudan department of Aquatic animal Health Management Sher-e-Bangla Agricultural University Dhaka Bangladesh

Fusobacterium nucleatum is an anaerobic, commensal, gram-negative oral bacterium that is carcinogenic and causes a wide range of human diseases. The present study focused on the analysis of the hypothetical protein, HMPREF3221_01179, derived from F. nucleatum strain MJR7757B, employing various computational methods to anticipate both its structure and functional characteristics. NCBI conserved domain analysis, NCBI BLASTp and MEGA Phylogenetic tree study characterize the target protein as an outer membrane efflux protein (ToIC family) which facilitate the bacterial transmembrane transport. With a molecular weight of 52120.02 Da, an isoelectric point (pI) of 8.33, and an instability index of 29.47, the protein is anticipated to exhibit good solubility in the extracellular space and crucial stability for pharmaceutical applications. The protein’s structure meets quality standards during the construction and refinement of its 3D model. The efflux inhibitor Arginine beta-naphthylamide exhibits a significant binding affinity (-7.1 kcal/mol) to the binding site of the target protein. The in-silico analysis improves the understanding of the protein and facilitates future investigations into therapeutic medication.

关键词： Fusobacterium nucleatum In Silico Bacteria Hypothetical Protein Molecular Docking

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