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Surgical anatomy of innervation of the gallbladder in humans and Suncus murinus with special reference to morphological understanding of gallstone formation after gastrectomy

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World Journal of Gastroenterology 2007年第14期13卷 2066-2071页

作者： Shuang-Qin Yi Tetsuo Ohta Akihiko Tsuchida Hayato Terayama Munekazu Naito Jun Li Heng-Xiao Wang Nozomi Yi Shigenori Tanaka Masahiro Itoh Department of Anatomy Tokyo Medical University 6-1-1 Shinjuku Shinjukuku Tokyo 160-8402 Japan Department of Gastroenterologie Surgery Kanazawa University 13-1 Takara-Machi Kanazawa 920-8420Japan Third Department of Surgery Tokyo Medical Kanazawa University 13-1 Takara-Machi Kanazawa 920-8420Japan Department of Anatomy and Neuroembryology Kanazawa University 13-1 Takara-Machi Kanazawa 920-8420Japan

AIM： To clarify the innervation of human gallbladder,with special reference to morphological understanding of gallstone formation after gastrectomy.METHODS： The liver, gallbladder and surrounding structures were immersed in a 10 mg/L solution of alizarin red S in ethanol to stain the peripheral nerves in cadavers （n = 10）. Innervation in the areas was completely dissected under a binocular microscope. Similarly,innervation in the same areas of 10 Suncus murinus （S. murinus） was examined employing whole mount immunohistochemistry.RESULTS： Innervation of the gallbladder occurred predominantly through two routes. One was from the anterior hepatic plexus, the innervation occurred along the cystic arteries and duct. Invariably this route passed through the hepatoduodenal ligament. The other route was from the posterior hepatic plexus, the innervation occurred along the cystic duct ventrally. This route also passed through the hepatoduodenal ligament *** results were obtained in S. murinus.CONCLUSION： The route from the anterior hepatic plexus via the cystic artery and/or duct is crucial for preserving gallbladder innervation. Lymph node dissection specifically in the hepatoduodenal ligament may affect the incidence of gallstones after ***, the route from the posterior hepatic plexus via the common bile duct and the cystic duct to the gallbladder should not be disregarded. Preservation of the plexus may attenuate the incidence of gallstone formation after gastrectomy.

关键词： Innervation Gallstone Hepatoduodenalligament Gastrectomy Whole mount immunohistochemistry

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Development of Analyses of Biological Steroids Using Chromatography——Special Reference to Vitamin D Compounds and Neurosteroids

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色谱 2003年第6期21卷 632-637页

作者： KazutakeShimada TatsuyaHigashi KunikoMitamura FacultyofPharmaceuticalSciences KanazawaUniversity13-1Takara-machiKanazawa920-0934Japan

Steroids comprise a large group of natural substances that must frequently be monitored in various biological materials. Due to the metabolic versatility of steroid molecules, extremely complex mixtures are often encountered, necessitating the use of a chromatographic procedure prior to measurement. In this article we present our work, that is, the development of analyses of biological steroids (especially vitamin D compounds and neurosteroids) using gas chromatography/mass spectrometry, high-performance liquid chromatography (including inclusion chromatography using cyclodextrin) and liquid chromatography/mass spectrometry.

关键词：类固醇化学分析气相色谱法质谱高效液相色谱维生素D 甾类化合物

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Surgical anatomy of the innervation of pylorus in human and Suncus murinus, in relation to surgical technique for pylorus-preserving pancreaticoduodenectomy

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World Journal of Gastroenterology 2006年第14期12卷 2209-2216页

作者： Shuang-Qin Yi Fei Ru Tetsuo Ohta Hayato Terayama Munekazu Naito Shogo Hayashi Sichen Buhe Nozomi Yi Takayoshi Miyaki Shigenori Tanaka Masahiro Itoh Department of Anatomy Tokyo Medical University 6-1-1 Shinjuku Shinjuku-ku Tokyo 160-8402 Japan Department of Medicine Johns Hopkins Medical Institutions 600 North Wolfe Street Baltimore Maryland 21287 United States Department of Gastroenterologic Surgery Kanaza- wa University 13-1 Takara-Machi Kanazawa 920-8420 Japan Department of Anatomy and Neuroembryology Kanazawa University 13-1 Takara-Machi Kanazawa 920-8420 Japan

AIM： To clarify the innervation of the antro-pyloric region in humans from a clinico-anatomical perspective. METHODS： The stomach, duodenum and surrounding structures were dissected in 10 cadavers, and immersed in a 10mg/L solution of alizarin red S in ethanol to stain the peripheral nerves. The distribution details were studied to confirm innervations in the above areas using a binocular microscope. Similarly, innervations in 10 Suncus murinus were examined using the method of whole-mount imm unohistochemistry. RESULTS： The innervation of the pyloric region in humans involved three routes： One arose from the anterior hepatic plexus via the route of the suprapyloric/supraduodenal branch of the right gastric artery; the second arose from the anterior and posterior gastric divisions, and the third originated from the posterior-lower region of the pyloric region, which passed via the infrapyloric artery or retroduodenal branches and was related to the gastroduodenal artery and right gastroepiploic artery. For Suncus murinus, results similar to those in humans were observed. CONCLUSION： There are three routes of innervation of the pyloric region in humans, wherein the route of the right gastric artery is most important for preserving pyloric region innervation. Function will be preserved by more than 80% by preserving the artery in pylorus-preserving pancreaticoduodenectomy （PPPD）. However, the route of the infrapyloric artery should not be disregarded. This route is related to several arteries （the right gastroepiploic and gastroduodenal arteries）, and the preserving of these arteries is advantageous for preserving pyloric innervation in PPPD. Concurrently, the nerves of Latarjet also play an important role in maintaining innervation of the antro-pyloric region in PPPD. This is why pyloric function is not damaged in some patients when the right gastric artery is dissected or damaged in PPPD.

关键词： Innervation Quality of life Pylorus-preserving pancreaticoduodenectomy Suncus murinus Whole mount immunohistochemistry

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T cell immunopathogenesis and immunotherapeutic strategies for chronic hepatitis B virus infection

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World Journal of Gastroenterology 2012年第20期18卷 2443-2451页

作者： Yukihiro Shimizu Gastroenterology Unit Takaoka City Hospital 4-1 Takara-machi Takaoka city Toyama 933-8550 Japan

Hepatitis B is caused by the host immune response and T cells play a major role in the immunopathogenesis. More importantly,T cells not only destroy hepatocytes infected by hepatitis B virus(HBV),but also control HBV replication or eradicate HBV in a noncytolytic ***,analysis of T cell immune response during acute and chronic HBV infection is important to develop a strategy for successful viral control,which could lead to immunotherapy for terminating persistent HBV *** have been many attempts at immunotherapy for chronic HBV infection,and some have shown promising *** viral load has been shown to suppress antiviral immune responses and immunoinhibitory signals have been recently elucidated, therefore,viral suppression by nucleos(t)ide analogs, stimulation of antiviral immune response,and suppression of the immunoinhibitory signals must be combined to achieve desirable antiviral effects.

关键词： T cells Immunopathogenesis Immunotherapy Hepatitis B virus infection

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Interaction with general transcription factor ⅡF (TFⅡF) is required for the suppression of activated transcription by RPB5-mediating protein(RMP)

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Cell Research 2003年第2期 111-120页

作者： DORJBAL DORJSUREN,SEISHI MURAKAMI Department of Molecular Oncology Cancer Research Institute Kanazawa University takara-machi 13-1 Kanazawa 920-0934 Japan Department of Molecular Oncology Cancer Research Institute Kanazawa University takara-machi 13-1 Kanazawa 920-0934 Japan

RMP was reported to regulate transcription via competing with HBx to bind the general transcription factor IIB (TFIIB) and interacting with RPB5 subunit of RNA polymerase Ⅱ as a corepressor of transcription regulator. However, our present research uncovered that RMP also regulates the transcription through interaction with the general transcription factors IIF (TFIIF), which assemble in the preinitiation complex and function in both transcription initiation and elongation. With in vitro pull-down assay and Far-Western analysis, we demonstrated that RMP could bind with bacterially expressed recombinant RAP30 and RAP74 of TFIIF subunits. In the immunoprecipitation assay in COS1 cells cotransfected with FLAG-tagged RMP or its mutants, GST-fused RAP30 and RAP74 were co-immunoprecipitated with RMP in approximately equal molar ratio, which suggests that RAP30 and RAP74 interact with RMP as a TFIIF complex. Interestingly both RAP30 and RAP74 interact with the same domain (D5) of the C-terminal RMP of 118-amin

关键词： general transcription factor IIF RPB5-mediating protein activated transcription RNA polymerase Ⅱ subunit 5.

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Boosting high-performance in Zr-rich side protonic solid oxide electrolysis cells by optimizing functional interlayer

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Green Energy & Environment 2025年第1期10卷 150-160页

作者： Chunmei Tang Ning Wang Sho Kitano Hiroki Habazaki Yoshitaka Aoki Siyu Ye Huangpu Hydrogen Energy Innovation Center School of Chemistry and Chemical EngineeringGuangzhou UniversityNo.230 Wai Huan Xi RoadGuangzhou510006China Graduate School of Chemical Sciences and Engineering Hokkaido UniversityN13W8Kita-kuSapporo060-8628Japan Faculty of Engineering Hokkaido UniversityN13W8Kita-kuSapporo060-8628Japan

Protonic solid oxide electrolysis cells(P-SOECs)are a promising technology for water electrolysis to produce green ***,there are still challenges related key materials and anode/electrolyte interface.P-SOECs with Zr-rich electrolyte,called Zr-rich side P-SOECs,possess high thermodynamically stability under high steam concentrations but the large reaction resistances and the current leakage,thus the inferior *** this study,an efficient functional interlayer Ba_(0.95)La_(0.05)Fe_(0.8)Zn_(0.2)O_(3-δ)(BLFZ)in-between the anode and the electrolyte is *** electrochemical performances of P-SOECs are greatly enhanced because the BLFZ can greatly increase the interface contact,boost anode reaction kinetics,and increase proton injection into *** a result,the P-SOEC yields high current density of 0.83 A cm^(-2) at 600℃ in 1.3 Vamong all the reported Zr-rich side *** work not only offers an efficient functional interlayer for P-SOECs but also holds the potential to achieve P-SOECs with high performances and long-term stability.

关键词： Functional interlayer Zr-rich side electrolyte Protonic solid oxide electrolysis cells Current density Faradaic efficiency

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Effect of Human Interleukin-8(IL-8) on Highighly Purified Murine Hematopoietic Progenitor Cells

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中国肿瘤生物治疗杂志 1995年第4期2卷 272-273页

作者： T,Zhang T.Ito S.Nakao N,Mukaida K,Matsushima Departmtntofpharmacology CancerResearchInstituteKanazawaUniversity13-1Takara-machiKanazawaIshikawa920Japan ThirdDepartmentofInternalMedicine SchoolofMedicineKanazawaUniversity13-1Takara-machiKanazawaIshikawa920Japan

Gene targeting of mouse homolog of interleukin-8 receptor (IL-8R) resulted in the increase of myeloid cells in the bone marrow and peripheral blood, sugesting a potential ro le of IL-8 mregulating hematopoiesis of the... 详细信息

关键词：白细胞介素-8 造血干细胞大鼠基因表达

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Velocity and Size of Droplets in Dense Region of Diesel Fuel Spray on Transient Needle Opening Condition

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Journal of Thermal Science 2005年第2期14卷 181-186页

作者： Hironobu UEKI Masahiro ISHIDA Daisaku SAKAGUCHI Nagasaki University 1-14 Bunkyo-machi Nagasaki 852-8521JapanNagasaki University 1-14 Bunkyo-machiNagasaki 852-8521JapanNagasaki University 1-14 Bunkyo-machiNagasaki 852-8521Japan

In order to investigate the effect of transient needle opening on early stage of spray behavior, simultaneous measurements of velocity and size of droplet were conducted by a newly developed laser 2-focus velocimeter (L2F). The micro-scale probe of the L2F was consisted of two foci with a distance of 36μm. The tested nozzle had a single hole with a diameter of 0.2 mm. The measurements of injection pressure, needle lift, and crank angle were synchronized with the spray measurement by the L2F at the position 10 mm downstream from the nozzle exit. It has been clearly shown that the velocity and size of droplet increase with needle valve opening and that the probability density distribution of droplet size can be fitted to the Nukiyama-Tanasawa distribution under the transient needle opening condition.

关键词： droplet disintegration optical characterization laser 2-focus velocimeter.

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Control of Secondary Flow in a Low Solidity Circular Cascade Diffuser

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Journal of Thermal Science 2012年第4期21卷 384-390页

作者： Daisaku Sakaguchi Takuji Fujii Hironobu Ueki Masahiro Ishida Hiroshi Hayami Nagasaki University 1-14 Bunkyo-machi Kyushu University (retired)

According to the previous experimental works on the low solidity circular cascade diffuser (LSD), a pressure recovery of a centrifugal blower was improved by the LSD significantly in a wide range of flow rate, and the pres-sure recovery was improved further by the LSD with a tandem cascade in comparison with the LSD with a sin-gle-row cascade. In the present study, the flow behavior in the LSD with the tandem cascade has been analyzed numerically by using the commercial CFD code of ANSYS-CFX12. It was shown clearly that the higher pressure recovery was achieved by applying the LSD with the tandem cascade, and the high pressure recovery is based on the high pressure rise in the vaneless space upstream of the LSD and the high blade loading of the front blade of the LSD. The high pressure recovery in the LSD could be achieved by controlling the flow separation on the suc-tion surface of the front blade and also on that of the rear blade due to formation of the favorable secondary flow and due to increase in mass flow passing through the slit section between the front and rear blades.

关键词： LSD (Low solidity circular cascade diffuser) Centrifugal blower Range enhancement Tandem cascade

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Multi-point Optimization of Recirculation Flow Type Casing Treatment in Centrifugal Compressors

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Journal of Thermal Science 2016年第3期25卷 231-241页

作者： Min Thaw Tun Daisaku Sakaguchi Nagasaki University 1-14 Bunkyo-machi 852-8521 Nagasaki Japan

High-pressure ratio and wide operating range are highly required for a turbocharger in diesel engines. A recirculation flow type casing treatment is effective for flow range enhancement of centrifugal compressors. Two ring grooves on a suction pipe and a shroud casing wall are connected by means of an annular passage and stable recirculation flow is formed at small flow rates from the downstream groove toward the upstream groove through the annular bypass. The shape of baseline recirculation flow type casing is modified and optimized by using a multi-point optimization code with a metamodel assisted evolutionary algorithm embedding a commercial CFD code CFX from ANSYS. The numerical optimization results give the optimized design of casing with improving adiabatic efficiency in wide operating flow rate range. Sensitivity analysis of design parameters as a function of efficiency has been performed. R is found that the optimized casing design provides optimized recirculation flow rate, in which an increment of entropy rise is minimized at grooves and passages of the rotating impeller.

关键词： Centrifugal Compressor Casing Treatment Multi-point Optimization Turbocharger

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