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Characterization of SOX3 Gene in an Ovoviviparous Teleost, Black Rockfish(Sebastes schlegeli)

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Journal of Ocean University of China 2019年第2期18卷 431-440页

作者： MA Liman WANG Wenji SHANG Renjie ZHANG Quanqi QI Jie WANG Zhigang school of Medicine Taizhou UniversityTaizhou 318000 China Key Laboratory of Marine Genetics and Breeding (Ocean University of China) Ministry of Education school of Life Science Taizhou UniversityTaizhou 318000 China

Evolutionary evidence suggests that Sox3, a member of the high-mobility-group(HMG) family of transcription factors, is an ancestral precursor of Sry and is involved in sex determination similar to Sry. However, there is limited information regarding the SOX3 gene of the black rockfish(Sebastes schlegeli). In this study, we first isolated SOX3 gene from the gonads of S. schlegeli by homology cloning. The full-length of S. schlegeli SOX3(SsSOX3) c DNA was 1386 bp, comprising a 906-bp open reading frame, which encodes a peptide showing 93.6% and 93.9% homology with the Sox3 proteins of Epinephelus coioides and Oryzias latipe, respectively. Comparison of the cDNA sequence of the Ss SOX3 gene with the corresponding genomic DNA fragment revealed that the SsSOX3 gene consists of a single exon. Phylogenetic analysis demonstrated the evolutionary relationship of Ss SOX3 with other known SOXB1 genes in fish and tetrapods. The promoter region contains binding sites of several transcriptional factors that might participate in the regulation of Ss SOX3 expression. Quantitative real-time PCR analysis indicated that SsSOX3 was expressed in all the investigated larval developmental stages from 1 to 35 days after birth and the level of expression gradually decreased as the development proceeded. SsSOX3 exhibited sexually dimorphic expression in adult gonads, with high expression in the ovary but low expression in the testis. In situ hybridization revealed that SsSOX3 was strongly expressed in oocytes and follicular cells of ovaries but slightly expressed in germ cells of testicular tissues. Therefore, this study suggests that Ss SOX3 plays an important role in oogenesis and ovary differentiation in S. schlegeli.

关键词： black rockfish promoter analysis Sebastes schlegeli sex determination sexual differentiation SOX3

Facile synthesis of indoles by K2CO3 catalyzed cyclization reaction of 2-ethynylanilines in water

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Chinese Chemical Letters 2017年第2期28卷 231-234页

作者： Zhi Chen Xiao-Xiao Shi Dong-Qin Ge Zhen-Zhen Jiang Qi-Qi Jin Hua-Jiang Jiang Jia-Shou Wu school of Pharmaceutical and Chemical Engineering taizhou University Taizhou 318000 China

The cyclization reaction of 2-ethynyl-N-sulfonylanilides proceeded efficiently in water with the presence of a catalytic amount of K2CO3 under transition metal-free condition to give indoles in high *** recovery and r... 详细信息

关键词： 2-Ethynylanilines Cyclization reaction Indoles Water Potassium carbonate

Local coordination and electronic interactions of Pd/MXene via dual‐atom codoping with superior durability for efficient electrocatalytic ethanol oxidation

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Carbon Energy 2024年第8期6卷 166-177页

作者： Zhangxin Chen Fan Jing Minghui Luo Xiaohui Wu Haichang Fu Shengwei Xiao Binbin Yu Dan Chen Xianqiang Xiong Yanxian Jin school of Pharmaceutical and Chemical Engineering Taizhou UniversityTaizhouZhejiangChina school of Sciences Zhejiang Sci‐Tech UniversityHangzhouZhejiangChina

Catalyst design relies heavily on electronic metal‐support interactions,but the metal‐support interface with an uncontrollable electronic or coordination environment makes it ***,we outline a promising approach for the rational design of catalysts involving heteroatoms as anchors for Pd nanoparticles for ethanol oxidation reaction(EOR)*** doped B and N atoms from dimethylamine borane(DB)occupy the position of the Ti_(3)C_(2) lattice to anchor the supported Pd *** electrons transfer from the support to B atoms,and then to the metal Pd to form a stable electronic center.A strong electronic interaction can be produced and the d‐band center can be shifted down,driving Pd into the dominant metallic state and making Pd nanoparticles deposit uniformly on the ***‐obtained Pd/DB–Ti_(3)C_(2) exhibits superior durability to its counterpart(∼14.6% retention)with 91.1% retention after 2000 cycles,placing it among the top single metal anodic ***,in situ Raman and density functional theory computations confirm that Pd/DB–Ti_(3)C_(2) is capable of dehydrogenating ethanol at low reaction energies.

关键词： durability electronic interactions ethanol oxidation heteroatom codoping Pd/MXene

Photonic Band Gap Properties of Three-Dimensional SiO2 Photonic Crystals

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Chinese Physics Letters 2010年第7期27卷 116-118页

作者：刘彦平闫志君李志刚李勤涛王印月 school of Physics and Electronic Engineering Taizhou University Taizhou 318000 school of Physical Science and Technology Lanzhou University Lanzhou 730000

Three-dimensional SiO2 photonic crystals （PhCs） are fabricated on quartz substrates by the vertical deposition method. Scanning electron microscopy measurement reveals that the samples exhibit an ordered close-packed arrangement of SiO2 spheres. It is found that the position of the [111] photonie band gap （PBG） shifts to a long wavelength （red shift） with increasing sphere size. Gap broadening effects are observed due to the presence of defects in the samples. Moreover, the optical properties of the PBG are very sensitive to the annealing temperature. Our results indicate that the optical properties of the PBG can be easily tuned in the visible region by appropriate experimental parameters, which will be useful for practical applications of PhC optical devices.

关键词： Optical properties Property Properties Sampling Microscopy Measurement

Repair of Second-Generation Recycled Fine Aggregate of Waste Concrete from Freeze-Thaw Environment by Carbonation Treatment

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Journal of Renewable Materials 2024年第1期12卷 187-201页

作者： Jie Huang Rongbin Jiang Xiaobo Sun Yingyong Shuai school of Building Engineering Taizhou Polytechnic CollegeTaizhou225300China

The reuse of waste recycled concrete from harsh environments has become a research hotspot in the field of *** study investigated the repair effect of carbonation treatment on second-generation recycled fine aggregate(SRFA)obtained from recycled fine aggregate concrete(RFAC)subjected to freeze-thaw(FT)*** and after carbonation,the properties of SRFA were *** second-generation recycled fine aggregate(CSRFA)at five substitution rates(0%,25%,50%,75%,100%)to replace SRFA was used to prepare carbonated second-generation recycled fine aggregate concrete(CSRFAC).The water absorption,porosity and mechanical properties of CSRFAC were tested,and its frost-resisting durability was *** results showed after carbonation treatment,the physical properties of SRFA was improved and met the requirements of II *** micro-hardness of the interfacial transition zone and attached mortar in CSRFA was 50.5%and 31.2%higher than that in SRFA,*** the increase of CSRFA replacement rate,the water absorption and porosity of CSRFAC gradually decreased,and the mechanical properties and frost resistance of CSRFAC were gradually *** treatment effectively repairs the damage of SRFA caused by FT cycles and improves its application potential.

关键词： Carbonation treatment repair freeze-thaw cycles second-generation recycled fine aggregate

Genome sequencing provides insights into Caprifoliaceae genome evolution and the mechanism underlying second blooming phenomenon in Heptacodium miconioides

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Horticultural Plant Journal 2024年第3期10卷 897-910页

作者： Yueling Li Zhongshuai Sun Zexin Jin Junmin Li Zhejiang Provincial Key Laboratory of Plant Evolutionary Ecology and Conservation Taizhou UniversityTaizhouZhejiang 318000China Institute of Ecology Taizhou UniversityTaizhouZhejiang 318000China school of Life Sciences Taizhou UniversityTaizhouZhejiang 318000China

Plants of the Caprifoliaceae family are widely cultivated worldwide as ornamental plants owing to their numerous,sweet-smelling,beautiful flowers and *** miconioides Rehd.,a member of the family,is endemic to eastern China and is cultivated as a popular ornamental plant in North America and European *** has a rather novel and beautiful trait of high horticultural value,that is,its sepals persist and enlarge,turning purplish ***,we report the chromosome-level genome assembly of *** to understand its evolution and floral *** 622.28 Mb assembled genome harbored a shared whole-genome duplication with a related species,Lonicera *** genomic analysis suggested that chromosome fission events following genome duplication underlie the unusual chromosome number of these two species,as well as chromosome fission of another five chromosomes in ***,giving rise to a haploid chromosome number of 14(versus 9 in ***).In addition,based on transcriptome and chloroplast genome analysis of 17 representative species in the Caprifoliaceae,we assumed that large structural variations in the chromosomes of *** were not caused by *** in the candidate genes of the MADS-box family were detected in the *** genome,including AP1-,AP3-,and SEPexpanded,which might underlie the sepal elongation and development in this *** current findings provided a critical resource for genome evolution studies in Caprifoliaceae and it was an example of how multi-omics data can elucidate the regulation of important ornamental traits.

关键词： Heptacodium miconioides Genome assembly Caprifoliaceae Chromosome fusion Sepal traits

Giant Magnetoimpedance Effect in a Sandwiched Structure of as-Quenched FeNiCrSiB Ribbons

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Chinese Physics Letters 2013年第6期30卷 197-200页

作者： CHEN Wei-Ping LI Zhi-Gang SHAO Xian-Yi ZHONG Wen-Wu LI Qin-Tao LIU Yan-Ping Zhejiang Provincial Key Laboratory for Cutting Tools School of Physics and Electronics EngineeringTaizhou UniversityTaizhou 318000

The giant magnetoimpedance(GMI)effect and effective permeability ratio in a QFR/Cu/QFR sandwiched structure are studied,where QFR stands for the as-quenched FeNiCrSiB amorphous *** GMI effects are obtained in the QFR/Cu/QFR sandwiched structure without *** maximum values of the longitudinal and transverse GMI ratios at 0.5 MHz are 282%and 408%,***,the maximum effective permeability ratios at 0.5 MHz are 326%and 1013%in longitudinal and transverse field,*** large GMI values are attributed to the high effective permeability of the sample due to the closed alternating current(ac)magnetic flux path in the sandwiched structure,and large permeability variation induced by the magnetic field.

关键词： sandwich transverse structure

Directional interpolation infinite element for dynamic problems in saturated porous media

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Earthquake Engineering and Engineering Vibration 2020年第3期19卷 625-635页

作者： Xiong Hao Chen Qingsheng Zhu Bingjian school of Civil Engineering and Architecture Taizhou UniversityTaizhou 318000China school of Civil Architecture and Environmental EngineeringHubei University of TechnologyWuhan 430068China

In this study,a directional interpolation infinite element suited to a saturated porous medium is presented to account for dynamic problems with semi-infinite or infinite *** employing the Galerkin method,the element property matrixes for a two-dimensional space are *** the governing differential equations for wave propagation in the saturated porous media,the analytical solutions for one-dimensional wave propagation are derived in *** the shape functions for the infinite element based on the analytical solutions are formulated and the dynamic stiffness matrix is presented in analytical form is *** a result,a fully coupled 2 D infinite element for the analysis of dynamic problems in unbounded saturated porous media is *** effectiveness and accuracy of the proposed element is well demonstrated by a comparison of the numerical results with known analytical solutions for 1 D and 2 D wave propagation *** results highlight that the proposed directional interpolation infinite element is useful and effective for addressing the dynamic problems with semi-infinite or infinite domain,with consideration of both compression and shear waves.

关键词： element saturated dynamic

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WORD MAGIC

Chinese Journal of Applied Linguistics 1999年第3期22卷 26-29页

作者： Zhao Xinmin Shandan no.1 middle school Shandan Gansu

This article presents a word game named"Word Magic",which is effective and efficient inavoiding word forgetting & decaying as well as helping students to improve their abilities in spelling,word building and so *** procedures and rules of the game are formulated together with the makingof the cards used in *** advantages of the game are also expounded.

关键词： WORD MAGIC Player down