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Dietary chenodeoxycholic acid improves growth performance and intestinal health by altering serum metabolic profiles and gut bacteria in weaned piglets

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Animal Nutrition 2021年第2期7卷 365-375页

作者： Min Song Fenglin Zhang Lin Chen Qiang Yang Han Su Xiaohua Yang Haiwen He Mingfa Ling Jisong Zheng Chen Duan Xumin Lai Mushui Pan Xiaotong Zhu Lina Wang Ping Gao Gang Shu Qingyan Jiang Songbo Wang Guangdong Provincial Key Laboratory of Animal Nutrition Control College of Animal ScienceSouth China Agricultural UniversityGuangzhou510642China National Engineering Research Center for Breeding Swine Industry and ALLTECH-SCAU Animal Nutrition Control Research Alliance South China Agricultural UniversityGuangzhou510642China Sericultural&Agri-Food Research institute Guangdong Academy of Agricultural SciencesKey Laboratory of Functional FoodsMinistry of AgricultureGuangdong Key Laboratory of Agricultural Products ProcessingGuangzhou510610China

Nutritional diarrhea and subsequent performance degradation in weaned piglets are major challenges for the pig *** acids(BA)can be added to the diet as *** experiment was conducted to investigate the effects of chenodeoxycholic acid(CDCA),a major primary BA,on growth performance,serum metabolic profiles and gut health in weaned piglets.A total of 72 healthy weaned piglets were randomly assigned to the control(CON)and the CDCA groups,which were feed a basal diet and the basal diet supplemented with 200 mg/kg CDCA for 30 d,*** results demonstrated that CDCA significantly increased final BW and average daily gain(ADG),decreased feed-to-gain(F:G)ratio and tended to reduce diarrhea *** addition,CDCA increased the villus height-to-crypt depth(V:C)ratio,elevated goblet cell numbers and the expression of tight junction proteins,suggesting the enhancement of intestinal barrier *** an emulsifier,CDCA increased jejunal lipase activity and the mRNA expression of pancreatic *** supplementation also altered the serum metabolic profiles,including increasing the levels of indole 3-acetic acid,N'-formylkynurenine and theobromine that were beneficial for gut ***,the relative abundance of 2 beneficial gut bacteria,Pre-votella 9 and Prevotellaceae TCG-001,were increased,whereas the relative abundance of a harmful bacteria,Dorea,was decreased in the gut of weaned piglets supplemented with ***,the altered serum metabolic profiles showed a strong correlation with the changed gut *** conclusion,CDCA improved the growth performance of weaned piglets by improving intestinal morphology and barrier function,and enhancing lipid digestion,accompanied by alterations of serum metabolic profiles,and changes in relative abundance of certain gut bacteria.

关键词： Chenodeoxycholic acid Weaned piglet Growth performance Gut health Serum metabolite Gut microbiota

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Validation of Landsat land surface temperature product in the conterminous United States using in situ measurements from SURFRAD, ARM, and NDBC sites

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International Journal of Digital Earth 2021年第5期14卷 640-660页

作者： Si-Bo Duan Zhao-Liang Li Wei Zhao Penghai Wu Cheng Huang Xiao-Jing Han Maofang Gao Pei Leng Guofei Shang Key Laboratory of agricultural Remote Sensing Ministry of Agriculture and Rural Affairs/Institute of Agricultural Resources and Regional PlanningChinese Academy of Agricultural SciencesBeijingPeople’s Republic of China School of Land science and Space Planning Hebei GEO University HebeiPeople’s Republic of China institute of Mountain Hazards and Environment Chinese Academy of SciencesChengduPeople’s Republic of China Anhui Province Key Laboratory of Wetland Ecosystem Protection and Restoration Anhui UniversityHefeiPeople’s Republic of China

Since 1982,Landsat series of satellite sensors continuously acquired thermal infrared images of the Earth’s land *** this study,Landsat 5,7,and 8 land surface temperature(LST)products in the conterminous United States from 2009 to 2019 were validated using in situ measurements collected at 6 SURFRAD(Surface Radiation Budget Network)sites,6 ARM(Atmospheric Radiation Measurement)sites,and 9 NDBC(National Data Buoy Center)*** results indicate that a relatively consistent performance among Landsat 5,7,and 8 LST products is obtained for most sites due to the consistent LST retrieval algorithm in conjunction with the same atmospheric compensation and land surface emissivity(LSE)correction methods for Landsat 5,7,and 8 *** bias and root mean square error(RMSE)of Landsat LST product are obtained at some vegetated sites due to incorrect LSE estimation where LSE is invariant with the increasing of normalized difference vegetation index(NDVI).Except for the sites with incorrect LSE estimation,a mean bias(RMSE)of the differences between Landsat LST and in situ LST is 1.0 K(2.1 K)over snow-free land surfaces,−1.1 K(1.6 K)over snow surfaces,and−0.3 K(1.1 K)over water surfaces.

关键词： Land surface temperature land surface emissivity validation Landsat

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Comparison of four methods to evaluate sperm DNA integrity between mouse caput and cauda epididymidis

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Asian Journal of Andrology 2012年第2期14卷 335-337页

作者： Serafin Perez-Cerezales： Alberto Miranda Alfonso Gutierrez-Adan Department of Animal Reproduction National Institute for Agricultural and Food Research and Technology Madrid 28040 Spain

It is well known that transit through the epididymis involves an increase in the compaction of sperm chromatin, which acquires fully condensed status at the caput epididymidis. The purpose of this study was to compare the terminal deoxyribonucleotidyl transferase-mediated dUTP nick end-labelling （TUNEL） assay, the comet assay, the sperm chromatin structure assay （SCSA） and the sperm chromatin dispersion （SCD） test by analysing spermatozoa from the caput and cauda epididymidis in order to demonstrate the ability of each technique to discriminate between different degrees of sperm maturity related to chromatin compaction and DNA fragmentation. Our results suggest that some populations of DNA-fragmented spermatozoa associated with immature sperm can only be identified using the comet assay and the SCSA but not with the SCD test or the TUNEL assay.

关键词： DNA damage mouse epididymis sperm chromatin sperm maturation the comet assay the sperm chromatin dispersion test the sperm chromatin structure assay TUNEL

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Gene Expression Profiling Related to Hyphal Growth in a Temperature-Sensitive Mutant of Magnaporthe oryzae

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Journal of Integrative Agriculture 2013年第12期12卷 2189-2196页

作者： LI Xue-song XU Fei WANG Hong-kai LIN Fu-cheng State Key Laboratory for Rice Biology Biotechnology InstituteZhejiang University Bioinformatics Lab Institute of Digital AgricultureZhejiang Academy of Agricultural Sciences

The rice blast,caused by fungus Magnaporthe oryzae,is a major constraint to the world food *** growth is the foundation of fungal development and proliferation of *** investigate genes involved in hyphal growth of this fungus,digital gene expression tag profiling was used to compare a previously generated temperature-sensitive mutant which defect at hyphae growth and reduction on pathogenicity,with its related wildtype strain.416 genes were detected as differential expression,178 of which were specifically expressed in Guy-11 but down-regulated expression in the *** classification analysis revealed the phenotype mutation may be mainly caused by a defection in translational and vacuole- related *** results and the protocol used will improve our knowledge on morphogenesis and promote the further study on *** pathogenesis.

关键词： differential expressed gene qRT-PCR gene ontology hyphal growth vacuole

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Effects of characteristic inhomogeneity of bamboo culm nodes on mechanical properties of bamboo fiber reinforced composite

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Journal of Forestry Research 2015年第4期26卷 1057-1060页

作者： Jinqiu Qi Jiulong Xie Wenji Yu Simin Chen College of Forestry Sichuan Agricultural University Research institute of Wood Industry Chinese Academy of Forestry

Dendrocalamus farinosus and Phyllostachys heterocycla bamboo logs were subjected to a novel treat- ment process for the preparation of bamboo fiber mats （BFMs）, and the obtained BFM were used to fabricate bamboo fiber reinforced composite （BFRC）. We studied the mechanical properties of the BFRCs manufactured from the mats with and without bamboo nodes. The pres- ence of nodes in BFM greatly reduced tensile strength, compressive strength, modulus of elasticity, and modulus of rupture of the BFRCs, while the BFRCs fabricated from BFMs with nodes possessed higher horizontal shear strength. Therefore, the nodes in bamboo culms were an important factor in the uniform distribution of mechanical properties, and BFMs should be homogeneously arranged to reduce the impact of nodes on the mechanical strengths of BFRCs.

关键词： Bamboo fiber reinforced composite - Culmnode Mechanical properties

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Study on the spatial differences and its time lag effect on climatic factors of the vegetation in the Longitudinal Range-Gorge Region

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Chinese science Bulletin 2007年第A2期52卷 42-49页

作者： BAO YaJing SONG GuoBao LI ZhengHai GAO JiXi LU HaiYan WANG HaiMei CHENG Yan XU Tian institute of Bio-Resources and Environment College of Life Science Dalian Nationalities University Dalian 116600 China College of Resources science and Technology Beijing Normal University Beijing 100875 China Innovation Base of Regional Ecology Chinese Research Academy of Environmental Sciences Beijing 100012 China institute of agricultural Resources and Regional Planning Chinese Academy of Agricultural Sciences Beijing 100081 China Inner Mongolia Municipality Observatory Huhhot 010051 China College of Ecological Environment Inner Mongolia Agricultural University Huhhot 010018 China College of Life science Inner Mongolia University Huhhot 010021 China

这篇文章包括温度和降水分析 NDVI 和二个气象学的数据的关联，并且探查回答植被的敏感到温度和降水变化的空间模式。由构造 NDVIr 索引，在在纵的范围峡区域(LRGR ) 的植被盖子和陆地使用之间的关系被揭示。结论如下：(1 ) 时间落后那... 详细信息

这篇文章包括温度和降水分析 NDVI 和二个气象学的数据的关联，并且探查回答植被的敏感到温度和降水变化的空间模式。由构造 NDVIr 索引，在在纵的范围峡区域(LRGR ) 的植被盖子和陆地使用之间的关系被揭示。结论如下：(1 ) 时间落后那植被回答到温度和降水变化的效果被证明。不同区域有相应于最大的关联系数的不同延期时间。根据降水和温度联合的反应时间，也就是，三种温度降水形式被定义在一样的时间的降水和温度，在温度以前的降水和在降水以前的温度。(2 ) 敏感的空间分发，哪个对温度的植被回答，被几个高、低的散布关联中心描绘。与温度，二个感觉迟钝的区域和二条敏感的带不同被形成介绍植被的反应给降水。(3 ) NDVIr 被构造，它能完美地在 LRGR 反映在气象学的因素和陆地使用状况之间的相应关系。

关键词：纵向岭谷区空间差异性气候植被

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A highly sensitive bio-barcode immunoassay for multi-residue detection of organophosphate pesticides based on fluorescence antiquenching

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Journal of Pharmaceutical Analysis 2022年第4期12卷 637-644页

作者： Lingyuan Xu Xiuyuan Zhang A.M.Abd El-Aty Yuanshang Wang Zhen Cao Huiyan Jia J.-Pablo Salvador Ahmet Hacimuftuoglu Xueyan Cui Yudan Zhang Kun Wang Yongxin She Fen Jin Lufei Zheng Baima Pujia Jing Wang Maojun Jin Bruce D.Hammock institute of Quality Standard and Testing Technology for Agro-Products Chinese Academy of Agricultural SciencesBeijing100081China Department of Pharmacology Faculty of Veterinary MedicineCairo UniversityGiza12211Egypt Department of Medical Pharmacology Medical FacultyAtaturk UniversityErzurum25240Türkiye institute of Livestock and Poultry Ningbo Academy of Agricultural SciencesNingboZhejiang315040China Nanobiotechnology for Diagnostics Group Instituto de Química Avanzada de CatalunaIQAC-CSICBarcelona08034Spain CIBER de Bioingeniería Biomateriales y Nanomedicina(CIBER-BBN)Madrid28029Spain Inspection and Testing Center of agricultural and Livestock Products of Tibet Department of Agriculture and Rural Affairs of Tibet Autonomous RegionLhasa850000China Department of Entomology&Nematology and the UC Davis Comprehensive Cancer Center University of CaliforniaDavisCA95616USA

Balancing the risks and benefits of organophosphate pesticides(OPs)on human and environmental health relies partly on their accurate measurement.A highly sensitive fluorescence anti-quenching multi-residue bio-barcode immunoassay was developed to detect OPs(triazophos,parathion,and chlorpyrifos)in apples,turnips,cabbages,and *** nanoparticles were functionalized with monoclonal antibodies against the tested *** oligonucleotides were complementarily hybridized with an RNA fluorescent label for signal *** detection signals were generated by DNA-RNA hybridization and ribonuclease H dissociation of the *** resulting fluorescence signal enables multiplexed quantification of triazophos,parathion,and chlorpyrifos residues over the concentration range of 0.01-25,0.01-50,and 0.1-50 ng/mL with limits of detection of 0.014,0.011,and 0.126 ng/mL,*** mean recovery ranged between 80.3% and 110.8% with relative standard deviations of 7.3%-17.6%,which correlate well with results obtained by liquid chromatography-tandem mass spectrometry(LC-MS/MS).The proposed bio-barcode immunoassay is stable,reproducible and reliable,and is able to detect low residual levels of multi-residue OPs in agricultural products.

关键词： Organophosphate pesticides Fluorescence anti-quenching Gold nanoparticles DNA-RNA hybridization DNA oligonucleotides Ribonuclease H

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Application of Chinese milk vetch affects rice yield and soil productivity in a subtropical double-rice cropping system

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Journal of Integrative Agriculture 2020年第8期19卷 2116-2126页

作者： CHEN Jing-rui QIN Wen-jing CHEN Xiao-fen CAO Wei-dong QIAN Guo-min LIU Jia XU Chang-xu institute of Soil&Fertilizer and Resources&Environment Jiangxi Academy of Agricultural SciencesNanchang 330200P.R.China institute of agricultural Resources and Regional Planning Chinese Academy of Agricultural SciencesBeijing 100081P.R.China Dengjiabu Original Rice Breeding Farm Yingtan 335000P.R.China

Green manure can be used as a substitute for chemical fertilizer without reducing rice *** studied the responses of soil fertility and rice yield to different combinations of Chinese milk vetch(CMV;Astragalus sinicus L.)and chemical fertilizer in a subtropical double-rice cropping *** goal is to reduce chemical fertilizer use and decrease environmental *** with the recommended rate of chemical fertilizer(CF),both early-and late-rice yields in the two treatments supplied with 15 and 22.5 Mg CMV ha^-1 plus 60%CF(represented as 60 A and 60 B,respectively)showed no significant differences while the two treatments supplied with 30 and 37.5 Mg CMV ha^-1 plus 60%CF(represented as 60 C and 60 D,respectively)showed significantly higher *** sustainable yield index(SYI)values in the 60 C and 60 D treatments with double-rice croppong system were significantly higher than those in other treatments(P0.05).Therefore,a relatively high Chinese milk vetch incorporation rate(≥30 Mg ha^-1)was more effective in improving the productivity and sustainability of paddy *** decreased coefficients of early-rice yield and the Chinese milk vetch incorporation rate in 2014 implied that the benefits of soil fertility and rice yield created by Chinese milk vetch input

关键词： Chinese milk vetch double-rice cropping system reduced chemical fertilizer sustainability

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cDNA Cloning of Heat Shock Protein Genes and Their Expression in an Indigenous Cryptic Species of the Whitefly Bemisia tabaci Complex from China

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Journal of Integrative Agriculture 2012年第2期11卷 293-302页

作者： YU Hao WAN Fang-hao GUO Jian-ying State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing 100081 P.R. China Department of Entomology Henan Institute of Science and Technology Xinxiang 453003 P.R.China

Thermal adaptation plays a fundamental role in shaping the distribution and abundance of insects,and heat shock proteins（Hsps）play important roles in the temperature adaptation of various *** better understand the temperature tolerance of the indigenous ZHJ2-biotype of whitefly Bemisia tabaci species complex,we obtained complete cDNA sequences for hsp90,hsp70,and hsp20 and analyzed their expression profiles under different high temperature treatments by real-time quantitative polymerase chain *** high temperature tolerance of *** ZHJ2-biotype was determined by survival rate after exposure to different high temperatures for 1 *** results showed that after 41°C heat-shock treatment for 1 h,the survival rates of ZHJ2 adults declined significantly and the estimated temperature required to cause 50% mortality（LT50）is 42.85°C for 1 *** for onset（Ton）or maximal（Tmax）induction of hsps expression in *** ZHJ2-biotype were 35 and 39°C（or 41°C）.Compared with previous studies,indigenous ZHJ2-biotype exhibits lower heat temperature stress tolerance and Ton（or Tmax）than the invasive B-biotype.

关键词： Bemisia tabaci biological invasion thermotolerance indigenous and invasive biotypes heat shock protein (hsp)

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Response of carbon footprint to plastic film mulch application in spring maize production and mitigation strategy

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Journal of Integrative Agriculture 2021年第7期20卷 1933-1943页

作者： CHEN Bao-qing Shahar BARAM DONG Wen-yi HE Wen-qing LIU En-ke YAN Chang-rong institute of Environment and Sustainable Development in Agriculture Chinese Academy of Agricultural SciencesBeijing 100081P.R.China institute for Soil Water and Environmental SciencesAgricultural Research Organization(ARO)Volcani Research CenterBet Dagan 50250Israel

Producing more food with a lower environmental cost is one of the most crucial challenges worldwide. Plastic mulching has developed as one of the most dominant practices to improve crop yields, however its impacts on greenhouse gas(GHG) emissions during the production life cycle of a crop are still unclear. The objective of this work is to quantify the impacts of plastic film on GHG emissions and to reduce GHG emissions with innovative agronomic practices. Carbon footprint per unit of area(CFa), per unit of maize grain yield(CFy), and per unit of economic output(CFe) were evaluated for three maize cultivation systems: a no mulch system, a conventional plastic mulching system(PM) and a biennial plastic mulching pattern, namely a ’one film for 2 years’ system(PM2), during 2015–2018 in a maize field located on the Loess Plateau of China. The results suggested that PM induced a 24% improvement in maize yields during the four experimental years compared to a no-mulch treatment(NM). However, PM dramatically increased the CFa by 69%, 59% of which was created by the input of the plastic film material, and 10% was created by increases in the soil N2O emissions. The yield improvements from PM could not offset the increases in CFa, and CFy and CFe were both increased by 36%. Shifting from PM to PM2 did not reduce crop yields, but it led to a 21% reduction in CFa and 23% reductions in CFy and CFe due to the reduced input amount of plastic film, decreased soil N2O emissions, and less diesel oil used for tillage. Compared to NM, CFy and CFe were only 5% higher in PM2. This study highlights the necessity of reducing the amount of plastic film input in the development of low-carbon agriculture and shifting from conventional PM cultivation to PM2 could be an efficient option for mitigating GHG emissions while sustaining high crop yields in plastic mulched fields.

关键词： carbon footprint greenhouse gas plastic film maize

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