检索结果-南通市图书馆

Design and construction of microbial cell factories based on Systems Biology

在线全文

学校读者我要写书评

暂无评论

Synthetic and systems Biotechnology 2023年第1期8卷 176-185页

作者： Wenlong Yan Zhibei Cao Mingzhu Ding Yingjin Yuan Frontier Science Center for Synthetic biology and Key Laboratory of systems Bioengineering(Ministry of Education) School of Chemical Engineering and TechnologyTianjin UniversityTianjin300072China Collaborative Innovation Center of Chemical Science and Engineering(Tianjin) Tianjin UniversityTianjin300072China

Environmental sustainability is an increasingly important issue in industry.As an environmentally friendly and sustainable way,constructing microbial cell factories to produce all kinds of valuable products has attracted more and more attention.In the process of constructing microbial cell factories,Systems Biology plays a crucial role.This review summarizes the recent applications of Systems Biology in the design and construction of microbial cell factories from four perspectives,including functional genes/enzymes discovery,bottleneck pathways identification,strains tolerance improvement and design and construction of synthetic microbial consortia.Systems Biology tools can be employed to identify functional genes/enzymes involved in the biosynthetic pathways of products.These discovered genes are introduced into appropriate chassis strains to build engineering microorganisms capable of producing products.Subsequently,Systems Biology tools are used to identify bottleneck pathways,improve strains tolerance and guide design and construction of synthetic microbial consortia,resulting in increasing the yield of engineered strains and constructing microbial cell factories successfully.

关键词： Microbial cell factories Systems Biology Functional genes/enzymes discovery Bottleneck pathways Strains tolerance Synthetic microbial consortia

Lessons Learned as President of the Institute for Systems Biology(2000–2018)

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Genomics, Proteomics & Bioinformatics 2018年第1期16卷 1-9页

作者： Leroy E. Hood Providence St. Joseph Health Seattle WA 98057 USA Institute for Systems Biology Seattle WA 98109 USA

I stepped down as president of the Institute for systems Biol- ogy （ISB） on Jan 1, 2018. As I think about my 17-year term as President, I am astounded at how much I have learned, not only about science but also abou... 详细信息

关键词： 21st-Century medicine systems medicine Scientific wellness P4 healthcare Systems Biology Personal, dense, dynamic data clouds

Opto-fluidically multiplexed assembly and micro-robotics

在线全文

学校读者我要写书评

暂无评论

Light(Science & Applications) 2024年第3期13卷 544-554页

作者： Elena Erben Weida Liao Antonio Minopoli Nicola Maghelli Eric Lauga Moritz Kreysing Max Planck Institute of Molecular Cell biology and Genetics Dresden 01307Germany Center for Systems Biology Dresden 01307Germany Institute of Biological and Chemical systems–Biological Information Processing.Karlsruhe Institute of Technology(KIT) Eggenstein-Leopoldshafen 76344Germany Department of Applied Mathematics and Theoretical Physics University of CambridgeCambridge CB30WAUK

Techniques for high-definition micromanipulations,such as optical tweezers,hold substantial interest across a wide range of disciplines.However,their applicability remains constrained by material properties and laser exposure.And while microfluidic manipulations have been suggested as an alternative,their inherent capabilities are limited and further hindered by practical challenges of implementation and control.Here we show that the iterative application of laser-induced,localized flow fields can be used for the relative positioning of multiple micro-particles,irrespectively of their material properties.Compared to the standing theoretical proposal,our method keeps particles mobile,and we show that their precision manipulation is non-linearly accelerated via the multiplexing of temperature stimuli below the heat diffusion limit.The resulting flow fields are topologically rich and mathematically predictable.They represent unprecedented microfluidic control capabilities that are illustrated by the actuation of humanoid micro-robots with up to 30 degrees of freedom,whose motions are sufficiently well-defined to reliably communicate personal characteristics such as gender,happiness and nervousness.Our results constitute high-definition micro-fluidic manipulations with transformative potential for assembly,micro-manufacturing,the life sciences,robotics and optohydraulically actuated micro-factories.

关键词： manipulation fluid assembly

Recent progress in MOFs-based nanozymes for biosensing

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Nano Research 2024年第1期17卷 39-64页

作者： Imamdin Chandio Yongjian Ai Lei Wu Qionglin Liang MOE Key Laboratory of Bioorganic Phosphorus Chemistry&Chemical biology Department of ChemistryLaboratory of Flexible Electronics TechnologyCenter for Synthetic and Systems BiologyTsinghua University-Peking University Joint Centre for Life SciencesTsinghua UniversityBeijing 100084China

Nanozymes are nanomaterials with enzyme-mimicking catalytic activity.Compared to natural enzymes,nanozymes show various properties such as easy to manufacture,stable,adjustable,and inexpensive.Nanozymes play key roles in biosensing,biocatalysis,and disease treatment.As an important kind of nanozymes,metal-organic framework(MOF)-based nanozymes are receiving a lot of attention due to their structural properties and composition.Rationally developing MOF with enzymes-like catalytic properties has opened new perspectives in biosensing.This review summarizes the up-to-date developments in synthesizing two-dimensional and three-dimensional MOF-based nanozymes and their applications in biosensing.Firstly,classification of nanozymes obtained by MOFs is categorized,and different properties of MOF-based nanozymes are described.Then,the distinctive applications of MOF-based nanozymes in identifying various analytes are thoroughly summarized.Finally,the recent challenges and progressive directions in this area are highlighted.

关键词： metal-organic frameworks nanozymes metal-organic framework (MOF)-based nanozymes biosensing analytes

Developmental Systems Biology flourishing on new technologies

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Genetics and Genomics 2008年第10期35卷 577-584页

作者： Jing-Dong J. Han Yi Liu Huiling Xue Kai Xia Hong Yu Shanshan Zhu Zhang Chen Wei Zhang Zheng Huang Chunyu Jin Bo Xian Jing Li Lei Hou Yixing Han Chaoqun Niu Timothy C. Alcon Chinese Academy of Sciences Key Laboratory of Molecular Developmental biology Center for Molecular Systems Biology Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China Key Laboratory of Machine Perception （Ministry of Education） Peking University Beijing 100871 China Bioinformatics and Computational biology Graduate Program Iowa State University Ames IA 50011-1040 USA

Organism development is a systems level process. It has benefited greatly from the recent technological advances in the field of Systems Biology. DNA microarray, phenome, interactome and transcriptome mapping, the new generation of deep sequencing technologies, and faster and better computational and modeling approaches have opened new frontiers for both systems biologists and developmental biologists to reexamine the old developmental biology questions, such as pattern formation, and to tackle new problems, such as stem cell reprogramming. As showcased in the International Developmental Systems Biology Symposium organized by Chinese Academy of Sciences, developmental Systems Biology is flourishing in many perspectives, from the evolution of developmental systems, to the underlying genetic and molecular pathways and networks, to the genomic, epigenomic and noncoding levels, to the computational analysis and modeling. We believe that the field will continue to reap rewards into the future with these new approaches.

关键词： developmental Systems Biology new technologies symposium

Cyclic Dinucleotide Self-Assembled Nanoparticles as a Carrier-Free Delivery Platform for STING-Mediated Cancer Immunotherapy

在线全文

学校读者我要写书评

暂无评论

CCS Chemistry 2024年第1期6卷 177-195页

作者： Lang Zhao Shao-Hua Zhuo Tian-Yang Wang Jun-Jun Wu Jing-Yun Su Wen-Hao Li Bo-Dou Zhang Yan-Mei Li Key Lab of Bioorganic Phosphorus Chemistry&Chemical biology Department of ChemistryTsinghua UniversityBeijing 100084 Beijing Institute for Brain Disorders Beijing 100069 Center for Synthetic and Systems Biology Tsinghua UniversityBeijing 100084

Cyclic dinucleotides(CDNs)are natural agonists of the stimulator of interferon genes(STING),which is an attractive immunotherapy target.Currently,CDNs and their derivatives are being investigated clinically.However,the poor bioavailability of exogenous CDNs has limited their application in immunotherapy.Although nanocarriers are widely used for cytosolic delivery of CDNs,their loading capacity is insufficient,and their complicated composition and purification process raises bio-compatibility concerns.Herein,we report a super-simplified CDN self-assembly strategy for carrier-free delivery of CDNs.In the presence of excess K^(+),CDNs form oligomers which further self-assemble with divalent metal ions(such as Mn^(2+))to form nanoparticles(NPs)in aqueous solution.We demonstrate that the self-assembled CDN NPs promote cellular uptake of CDNs and enhance tumor immunogenicity by remodeling the tumor microenvironment,inducing immunogenic tumor cell death and increasing tumorinfiltrating lymphocytes,which is conducive to the generation of tumor neoantigen-specific T-cell responses.We also demonstrate that the use of CDN NPs alone or in combination with immune checkpoint blockades inhibits tumor growth,highlighting the fact that CDN NPs are a potent platform for cancer immunotherapy.

关键词： cyclic dinucleotide STING carrierfree delivery tumor immunogenicity cancer immunotherapy

Building digital life systems for future biology and medicine

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Quantitative biology 2023年第3期11卷 207-213页

作者： Xuegong Zhang Lei Wei Rui Jiang Xiaowo Wang Jin Gu Zhen Xie Hairong Lv MOE Key Lab of Bioinformatics and Bioinformatics Division of BNRIST Department of AutomationTsinghua UniversityBeijing 100084China Center of Synthetic and Systems Biology Tsinghua UniversityBeijing 100084China School of Life Sciences and School of Medicine Tsinghua UniversityBeijing 100084China

The rapid development of biological technology (BT) and information technology (IT) especially of genomics and artificial intelligence (AI) is bringing great potential for revolutionizing future medicine. We propose the concept and framework of Digital Life systems or dLife as a new paradigm to unleash this potential. It includes the multi-scale and multi-granule measure and representation of life in the digital space, the mathematical and/or computational modeling of the biology behind physiological and pathological processes, and ultimately cyber twins of healthy or diseased human body in the virtual space that can be used to simulate complex biological processes and deduce effects of medical treatments. We advocate that dLife is the route toward future AI precision medicine and should be the new paradigm for future biological and medical research.

关键词： digital life systems digital twin aritificial intelligence precision medicine

Computational Systems Biology for omics data analysis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Molecular Cell biology 2019年第8期11卷 631-632页

作者： Luonan Chen Key Laboratory of Systems Biology Center for Excellence in Molecular Cell Science Shanghai Institute of Biochemistry and Cell Biology Chinese Academy of Sciences Shanghai 200031 China

Recent trend on biological data at a molecular level is omics data analysis for both bulk and single cells, in eluding genomics, proteomics, metabolomics, and epigenetics data (Wang and Zhang, 2017;Zhang et al., 2017;Zhao and Li, 2017;Cheng and Leung, 2018). Rapid accumulation of such high-dimensional biological data is driving the system-level study from describing complex phenomena to understanding molecular mechanisms (Park et al., 2018;Sun et al., 2018) and from analyzi ng in dividual components to understanding their networks and systems (Chen et al., 2009;Chen, 2017).

关键词： Computational systems biology omics data analysis

Systems Biology in the frontier of cancer research: a report of the Second International Workshop of Cancer Systems Biology

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Journal of Cancer 2012年第9期31卷 409-412页

作者： Juan Cui Yan-Chun Liang Ying Xu Computational Systems Biology Laboratory Department of Biochemistry and Molecular Biology University of Georgia Athens GA 30602-7229 USA College of Computer Science and Technology Jilin University Changchun Jilin 130012 P. R. China.

The report summarizes the Second International Workshop of Cancer Systems Biology held on July 5-6, 2012 in Changchun, China. The goal of the workshop was to bring together cancer researchers with different backgrounds to share their views about cancer and their experiences in fighting against cancer, and to gain new and systems-level understanding about cancer formation, progression, diagnosis, and treatment through exchanging ideas.

关键词：系统生物学国际研讨会抗癌症研究人员