检索结果-南通市图书馆

Phosphorylation of WHIRLY1 by CIPK14 Shifts Its Localization and Dual Functions in Arabidopsis

Molecular Plant 2017年第5期10卷 749-763页

作者： Yujun Ren Yanyun Li Youqiao Jiang Binghua Wu Ying Miao center for Molecular Cell and systems biology Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology College of Life Sciences Fujian Agriculture and Forestry University Fuzhou 350002 China These authors contributed equally to this article

Plastid-to-nucleus retrograde signaling is critical for normal growth and development in plants. The dualfunction and dual-located ssDNA binding protein WHIRLY1 （WHY1） has been proposed to coordinate the retrograde signaling from plastids to the nucleus. However, the regulatory mechanism governing the functional switch of WHY1 for mediating plastid-to-nucleus retrograde signaling remains unknown. Here, we report that the Calcineurin B-Like-Interacting Protein Kinase14 （CIPK14） interacts with and phosphorylates WHY1 in Arabidopsis. Phosphorylation of WHY1 results in increased accumulation in the nucleus and enhanced binding with the promoter of WRKY53, which encodes a key transcription factor regulating leaf senescence in Arabidopsis. Transgenic plants overexpressing CIPK14 showed an increased nuclear isoform but decreased plastid isoform of WHY1, among which 95% of transgenic lines showed the stay-green phenotype and 5% of lines showed the variegated pale-green phenotype. Interestingly, the phenotypes of both types of transgenic plants could be recovered by overexpression of plastid-form WHY1. In contrast, knockdown of ClPK14 caused early senescence and even seedling-lethal phenotypes along with elevated expression of senescence-related genes such as WRKY53, SAG12, and NDHF but decreased expression of MER11, RAD50, and POR genes, which could be rescued by overexpression of CIPK14 but not by overexpressing plastid-form or nuclear-form WHY1; the stay-green plants overexpressing ClPK14 showed reduced expression of WRKY53, SAG12, NDHF, and large plastid rRNA. Consistently, the accu- mulation of nuclear-form WHY1 was significantly reduced in the CIPK14 knockdown lines, resulting in a low ratio of nuclear-/plastid-form WHY1. Taken together, our results demonstrate that CIPK14 regu- lates the phosphorylation and organeUar distributions of WHY1 and pinpoint that ClPK14 may function as a cellular switch between leaf senescence and plastid development for coordinating the int

关键词： WHIRLY1 CIPK14 Retrograde Signalling Leaf Senescence Plastid Development

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

VASC: Dimension Reduction and Visualization of Single-cell RNA-seq Data by Deep Variational Autoencoder

引用

Genomics, Proteomics & Bioinformatics 2018年第5期16卷 320-331页

作者： Dongfang Wang Jin Gu MOE Key Laboratory of Bioinformatics BNRIST Bioinformatics Division & Center for Synthetic and Systems BiologyDepartment of Automation Tsinghua University

Single-cell RNA sequencing(scRNA-seq) is a powerful technique to analyze the transcriptomic heterogeneities at the single cell level. It is an important step for studying cell subpopulations and lineages, with an effective low-dimensional representation and visualization of the original scRNA-Seq data. At the single cell level, the transcriptional fluctuations are much larger than the average of a cell population, and the low amount of RNA transcripts will increase the rate of technical dropout events. Therefore, scRNA-seq data are much noisier than traditional bulk RNA-seq data. In this study, we proposed the deep variational autoencoder for scRNA-seq data(VASC), a deep multi-layer generative model, for the unsupervised dimension reduction and visualization of scRNA-seq data. VASC can explicitly model the dropout events and find the nonlinear hierarchical feature representations of the original data. Tested on over 20 datasets, VASC shows superior performances in most cases and exhibits broader dataset compatibility compared to four state-of-the-art dimension reduction and visualization methods. In addition, VASC provides better representations for very rare cell populations in the 2D visualization. As a case study, VASC successfully re-establishes the cell dynamics in pre-implantation embryos and identifies several candidate marker genes associated with early embryo development. Moreover, VASC also performs well on a 10× Genomics dataset with more cells and higher dropout rate.

关键词： Single cell RNA sequencing Deep variational autoencoder Dimension reduction Visualization Dropout

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Small heat shock protein B8:from cell functions to its involvement in diseases and potential therapeutic applications

引用

Neural Regeneration Research 2025年第10期20卷 2872-2886页

作者： Marta Chierichetti Riccardo Cristofani Valeria Crippa Veronica Ferrari Marta Cozzi Elena Casarotto Paola Pramaggiore Laura Cornaggia Guglielmo Patelli Ali Mohamed Margherita Piccolella Mariarita Galbiati Paola Rusmini Barbara Tedesco Angelo Poletti Laboratory of Experimental biology Dipartimento di Scienze Farmacologiche e Biomolecolari“Rodolfo Paoletti”(DiSFeB)Universitàdegli Studi di MilanoMilanItaly

Heat shock protein family B(small)member 8(HSPB8)is a 22 kDa ubiquitously expressed protein belonging to the family of small heat shock ***8 is involved in various cellular mechanisms mainly related to proteotoxic stress response and in other processes such as inflammation,cell division,and ***8 binds misfolded clients to prevent their aggregation by assisting protein refolding or degradation through chaperone-assisted selective *** line with this function,the pro-degradative activity of HSPB8 has been found protective in several neurodegenerative and neuromuscular diseases characterized by protein misfolding and *** cancer,HSPB8 has a dual role being capable of exerting either a pro-or an anti-tumoral activity depending on the pathways and factors expressed by the model of cancer under ***,HSPB8 exerts a protective function in different diseases by modulating the inflammatory response,which characterizes not only neurodegenerative diseases,but also other chronic or acute conditions affecting the nervous system,such as multiple sclerosis and intracerebellar *** note,HSPB8 modulation may represent a therapeutic approach in other neurological conditions that develop as a secondary consequence of other *** is the case of cognitive impairment related to diabetes mellitus,in which HSPB8 exerts a protective activity by assuring mitochondrial *** review aims to summarize the diverse and multiple functions of HSPB8 in different pathological conditions,focusing on the beneficial effects of its ***-based and alternative therapeutic approaches targeting HSPB8 and its regulated pathways will be discussed,emphasizing how new strategies for cell and tissue-specific delivery represent an avenue to advance in disease treatments.

关键词： aggregation autophagy cancer chaperone chaperone-assisted selective autophagy cognitive impairment HSPB8 neuroinflammation neuromuscular diseases therapy

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Computational Identification of Active Enhancers in Model Organisms

引用

Genomics, Proteomics & Bioinformatics 2013年第3期11卷 142-150页

作者： Chengqi Wang Michael Q. Zhang Zhihua Zhang CAS Key Laboratory of Genome Sciences and Information Beijing Institute of Genomics Chinese Academy of Sciences Department of Molecular Cell biology Center for Systems Biology University of Texas at Dallas Bioinformatics Division Center for Synthetic and Systems Biology TNLIST Tsinghua University

As a class of cis-regulatory elements, enhancers were first identified as the genomic regions that are able to markedly increase the transcription of genes nearly 30 years ago. Enhancers can regulate gene expression in a cell-type specific and developmental stage specific manner. Although experimental technologies have been developed to identify enhancers genome-wide, the design principle of the regulatory elements and the way they rewire the transcriptional regulatory network tempo-spatially are far from clear. At present, developing predictive methods for enhanc- ers, particularly for the cell-type specific activity of enhancers, is central to computational biology. In this review, we survey the current computational approaches for active enhancer prediction and discuss future directions.

关键词： Enhancer identification Active enhancer recognition Histone modification mark Transcription factor

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

LABEL-FREE DETECTION OF PROTEIN MICROARRAY WITH HIGH THROUGHPUT SURFACE PLASMON RESONANCE IMAGING(SPRI)

引用

Journal of Innovative Optical Health Sciences 2008年第1期1卷 107-114页

作者： SHUKUAN XU GUOLIANG HUANG CHENG DENG JIANG ZHU CHAO HAN XIAOYONG YANG Medical systems biology Research center Tsinghua University School of Medicine Beijing China National Engineering Research center for Beijing Biochip Technology BeijingChina

A surface plasmon resonance imaging(SPRI)system was developed for the discrimination of proteins on a gold *** a label-free and high-throughput technique,SPRI enables simultaneously monitoring of the biomolecular interactions at low *** used SPRI as a label-free and parallel method to detect different proteins based on protein *** Serum Albumin(BSA),Casein and Immunoglobulin G(IgG)were immobilized onto the Au surface of a gold-coated glass chip as spots forming a 6×6 *** proteins can be discriminated directly by changing the incident angle of *** reproducibility for label-free detection of protein molecules was *** SPRI platform represents a simple and robust method for performing high-sensitivity detection of protein microarray.

关键词： Surface plasmon resonance imaging protein microarray label-free highthroughput

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

New insights into tumor dormancy:Targeting DNA repair pathways

引用

World Journal of Clinical Oncology 2015年第5期6卷 80-88页

作者： Elizabeth B Evans Shiaw-Yih Lin Department of systems biology the University of Texas MD Anderson Cancer Center

Over the past few decades, major strides have advanced the techniques for early detection and treatment of cancer. However, metastatic tumor growth still accounts for the majority of cancer-related deaths worldwide. In fact, breast cancers are notorious for relapsing years or decades after the initial clinical treatment, and this relapse can vary according to the type of breast cancer. In estrogen receptor-positive breast cancers, late tumor relapses frequently occur whereas relapses in estrogen receptor-negative cancers or triple negative tumors arise early resulting in a higher mortality risk. One of the main causes of metastasis is tumor dormancy in which cancer cells remain concealed, asymptomatic, and untraceable over a prolonged period of time. Under certain conditions, dormant cells can re-enter into the cell cycle and resume proliferation leading to recurrence. However, the molecular and cellular regulators underlying this transition remain poorly understood. To date, three mechanisms have been identified to trigger tumor dormancy including cellular, angiogenic, and immunologic dormancies. In addition, recent studies have suggested that DNA repair mechanisms may contribute to the survival of dormant cancer cells. In this article, we summarize the recent experimental and clinical evidence governing cancer dormancy. In addition, we will discuss the role of DNA repair mechanisms in promoting the survival of dormant cells. This information provides mechanistic insight to explain why recurrence occurs, and strategies that may enhance therapeutic approaches to prevent disease recurrence.

关键词： Quiescence Homologous recombination Non-homologous end joining Tumor dormancy DNA repair

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Compensation of ACE2 Function for Possible Clinical Management of 2019-nCoV-Induced Acute Lung Injury

引用

Virologica Sinica 2020年第3期35卷 256-258页

作者： Yuntao Wu National center for Biodefense and Infectious Diseases School of Systems BiologyGeorge Mason UniversityManassasVA 20110USA

The 2019-nCoV viral infection causes clusters of severe respiratory illness such as an acute respiratory distress syndrome(ARDS)similar to that caused by SARS-CoV(severe acute respiratory syndrome coronavirus)(Huang e... 详细信息

关键词： ACE2 acute respiratory

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Molecular bases of morphologically diffused tumors across multiple cancer types

引用

National Science Review 2022年第11期9卷 65-67页

作者： Dingyun Liu Feiyang Xing Yueying Wang Jun Xiao Zheng An Ying Xu College of Computer Science and Technology Jilin University center for Cancer systems biology China-Japan Union Hospital of Jilin University School of Life Sciences Jilin University China Computational systems biology Lab Department of Biochemistry and Molecular Biology and Institute of BioinformaticsUniversity of Georgia

Diffuse gastric cancer (DGC) and intestinal gastric cancer (IGC) are the two main gastric cancer subtypes [1]. Morphologically, DGC consists of numerous disjoint tiny tumors while IGC forms one or a few solid tumors (Supplementary Supplementary Fig. S1)[1,2]. In-depth studies have discovered that DGCs generally have an ill-defined or missing glandular structure and reduced cell–cell adhesion,

关键词： identification enantioselective semisynthesis (−)-anisomelic acid oral agent anti-SARS-CoV-2

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Stochasticity in the yeast mating pathway

引用

Chinese Physics B 2009年第5期18卷 2082-2095页

作者：王宏利傅正平徐昕航欧阳颀 State Key Laboratory for Mesoscopic Physics School of PhysicsPeking University The Beijing-Hong Kong-Singapore Joint center for Nonlinear and Complex systems(PKU) center for Theoretical biology Peking University

We report stochastic simulations of the yeast mating signal transduction pathway. The effects of intrinsic and external noise, the influence of cell-to-cell difference in the pathway capacity, and noise propagation in the pathway have been examined. The stochastic temporal behaviour of the pathway is found to be robust to the influence of inherent fluctuations, and intrinsic noise propagates in the pathway in a uniform pattern when the yeasts are treated with pheromones of different stimulus strengths and of varied fluctuations. In agreement with recent experimental findings, extrinsic noise is found to play a more prominent role than intrinsic noise in the variability of proteins. The occurrence frequency for the reactions in the pathway are also examined and a more compact network is obtained by dropping most of the reactions of least occurrence.

关键词： noise stochastic simulation pheromone response mating pathway of yeast

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The crossroads of receptor-mediated signaling and endocytosis in plants

引用

Journal of Integrative Plant biology 2018年第9期60卷 827-840页

作者： Lucas Alves Neubus Claus Daniel V.Savatin Eugenia Russinova Department of Plant Biotechnology and Bioinformatics Ghent University9052 GhentBelgium center for Plant systems biology VIB9052 GhentBelgium

Plants deploy numerous plasma membrane receptors to sense and rapidly react to environmental changes. Correct localization and adequate protein levels of the cell-surface receptors are critical for signaling activation and modulation of plant development and defense against pathogens. After ligand binding, receptors are internalized for degradation and signaling attenuation. However, one emerging notion is that the Iigand-induced endocytosis of reCeptor complexes is important for the signal duration, amplitude, and specificity. Recently, mutants of maior endocytosis players, including clathrin and dynamin, have been shown to display defects in activation of a subset of signal transduction pathways, implying that signaling in plants might not be solely restricted to the plasma membrane. Here, we summarize the up-to-date knowledge of receptor complex endocytosis and its effect on the signaling outcome, in the context of plant development and immunity.

关键词： The crossroads of receptor-mediated signaling and endocytosis in plants BRI PM

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：