检索结果-南通市图书馆

Composition,function,and timing:exploring the early‑life gut microbiota in piglets for probiotic interventions

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Journal of Animal Science and biotechnology 2024年第2期15卷 659-678页

作者： Jianping Quan Cineng Xu Donglin Ruan Yong Ye Yibin Qiu Jie Wu Shenping Zhou Menghao Luan Xiang Zhao Yue Chen Danyang Lin Ying Sun Jifei Yang Enqin Zheng Gengyuan Cai Zhenfang Wu Jie Yang College of Animal Science and National Engineering Research Center for Breeding Swine Industry South China Agricultural UniversityGuangzhouGuangdongPeople’s Republic of China Guangdong Provincial Key laboratory of Agro‑Animal Genomics and molecular Breeding South China Agricultural UniversityGuangzhouGuangdongChina Yunfu Subcenter of Guangdong laboratory for Lingnan Modern Agriculture YunfuGuangdongChina National Engineering Research Center for Breeding Swine Industry Wens Foodstuff Group Co.Ltd.YunfuGuangdongPeople’s Republic of China

Background The establishment of a robust gut microbiota in piglets during their early developmental stage holds the potential for long-term advantageous ***,the optimal timeframe for introducing probiotics to achieve this outcome remains *** In the context of this investigation,we conducted a longitudinal assessment of the fecal microbiota of 63 piglets at three distinct pre-weaning time ***,we gathered vaginal and fecal samples from 23 *** 16S rRNA gene and metagenomic sequencing methodologies,we conducted a comprehensive analysis of the fluctuation patterns in microbial composition,functional capacity,interaction networks,and colonization resistance within the gut microbiota of *** the piglets progressed in age,discernible modifications in intestinal microbial diversity,composition,and function were observed.A source-tracking analysis unveiled the pivotal role of fecal and vaginal microbiota derived from sows in populating the gut microbiota of neonatal *** D21,the microbial interaction network displayed a more concise and efficient configuration,accompanied by enhanced colonization resistance relative to the other two time ***,we identified three strains of Ruminococcus *** D10 as potential candidates for improving piglets’weight gain during the weaning *** The findings of this study propose that D10 represents the most opportune juncture for the introduction of external probiotic interventions during the early stages of piglet *** investigation augments our comprehension of the microbiota dynamics in early-life of piglets and offers valuable insights for guiding forthcoming probiotic interventions.

关键词： Colonization resistance Intestinal microbiota Piglet Weaning Weight gain

Plant regeneration in the new era:from molecular mechanisms to biotechnology applications

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2024年第7期67卷 1338-1367页

作者： Chunli Chen Yuxin Hu Momoko Ikeuchi Yuling Jiao Kalika Prasad Ying Hua Su Jun Xiao Lin Xu Weibing Yang Zhong Zhao Wenkun Zhou Yun Zhou Jian Gao Jia-Wei Wang National Key laboratory for Germplasm Innovation&Utilization of Horticultural Crops Hubei Hongshan LaboratoryHuazhong Agricultural UniversityWuhan 430070China College of Life Science and Technology Huazhong Agricultural UniversityWuhan 430070China Key laboratory of Plant molecular Physiology Institute of BotanyChinese Academy of Sciences(CAS)China National Botanical GardenBeijing 100093China Division of Biological Sciences Graduate School of Science and TechnologyNara Institute of Science and TechnologyNara 630-0192Japan State Key laboratory of Protein and Plant Gene Research School of Life SciencesPeking UniversityBeijing 100871China Peking-Tsinghua Center for Life Sciences Center for Quantitative BiologyAcademy for Advanced Interdisciplinary StudiesPeking UniversityBeijing 100871China Indian Institute of Science Education and Research Pune 411008IndiaThiruvananthapuram 695551India State Key laboratory of Wheat Improvement College of Life SciencesShandong Agricultural UniversityTai’an 271018China Sino-German Joint Research Center on Agricultural Biology Shandong Agricultural UniversityTai’an 271018China Key laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental Biology(IGDB)CASBeijing 100101China CAS-JIC Centre of Excellence for Plant and Microbial Science(CEPAMS) IGDBCASBeijing 100101China National Key laboratory of Plant molecular Genetics CEMPSInstitute of Plant Physiology and Ecology(SIPPE)CASShanghai 200032China CEPAMS SIPPECASShanghai 200032China Hefei National laboratory for Physical Sciences at the Microscale CEMPSSchool of Life SciencesDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230027China State Key laboratory of Plant Environmental Resilience College of Biological SciencesChina Agricultural UniversityBeijing 100193China Department of Botany and Plant Pathology and Center for Plant Biology Purdue UniversityWest Lafayette 47907USA School of Life Science and Technology Shang

Plants or tissues can be regenerated through various *** animal regeneration,cell totipotency and pluripotency are the molecular basis of plant *** systematic studies on Arabidopsis thaliana gradually unravel the fundamental mechanisms and principles underlying plant ***,plant hormones,cell division,epigenetic remodeling,and transcription factors play crucial roles in reprogramming somatic cells and reestablishing meristematic *** research on basal non-vascular plants and monocot crops has revealed that plant regeneration differs among species,with various plant species using distinct mechanisms and displaying significant differences in regenerative *** multi-omics studies at the single-cell level,tracking plant regeneration processes in real-time,and deciphering the natural variation in regenerative capacity will ultimately help understand the essence of plant regeneration,improve crop regeneration efficiency,and contribute to future crop design.

关键词： apical meristem model species regeneration phytohormone plant cell reprogramming somatic embryogenesis

Identification and validation of novel prognostic fatty acid metabolic gene signatures in colon adenocarcinoma through systematic approaches

在线全文

学校读者我要写书评

暂无评论

Oncology Research 2024年第2期32卷 297-308页

作者： HENG ZHANG WENJING CHENG HAIBO ZHAO WEIDONG CHEN QIUJIE ZHANG QING-QING YU Department of laboratory Shandong Daizhuang HospitalJining272051China Jining No.1 People’s Hospital Shandong First Medical UniversityJining272000China

Colorectal cancer(CRC)belongs to the class of significantly malignant tumors found in ***,dysregulated fatty acid metabolism(FAM)has been a topic of attention due to its modulation in cancer,specifically ***,the regulatory FAM pathways in CRC require comprehensive ***:The clinical and gene expression data of 175 fatty acid metabolic genes(FAMGs)linked with colon adenocarcinoma(COAD)and normal cornerstone genes were gathered through The Cancer Genome Atlas(TCGA)-COAD corroborating with the molecular Signature Database v7.2(MSigDB).Initially,crucial prognostic genes were selected by uni-and multi-variate Cox proportional regression analyses;then,depending upon these identified signature genes and clinical variables,a nomogram was ***,to assess tumor immune characteristics,concomitant evaluation of tumor immune evasion/risk scoring were ***:A 8-gene signature,including ACBD4,ACOX1,CD36,CPT2,ELOVL3,ELOVL6,ENO3,and SUCLG2,was generated,and depending upon this,CRC patients were categorized within high-risk(H-R)and low-risk(L-R)***,risk and age-based nomograms indicated moderate discrimination and good *** data confirmed that the 8-gene model efficiently predicted CRC patients’***,according to the conjoint analysis of tumor immune evasion and the risk scorings,the H-R cohort had an immunosuppressive tumor microenvironment,which caused a substandard ***:This investigation established a FAMGs-based prognostic model with substantially high predictive value,providing the possibility for improved individualized treatment for CRC individuals.

关键词： Fatty acid metabolism Colorectal cancer Gene signatures Machine learning

GhWDL3 is involved in the formation and development of fiber cell morphology in upland cotton(Gossypium hirsutum L.)

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Cotton Research 2024年第1期7卷 58-68页

作者： CHEN Baojun TIAN Zailong FU Guoyong ZHANG Ai SUN Yaru WANG Jingjing PAN Zhaoe LI Hongge HU Daowu XIA Yingying HE Shoupu DU Xiongming Zhengzhou Research Base National Key Laboratory of Cotton Bio-Breeding and Integrated UtilizationZhengzhou UniversityZhengzhouHenan 450001China National Key laboratory of Cotton Bio-Breeding and Integrated Utilization Institute of Cotton ResearchChinese Academy of Agricultural SciencesAnyangHenan 455000China National Supercomputing Center in Zhengzhou Zhengzhou UniversityZhengzhouHenan 450001China

Background Cotton fiber is a model tissue for studying microtubule-associated proteins(MAPs).The Xklp2(TPX2)proteins that belong to the novel MAPs member mainly participate in the formation and development of microtubule(MT).However,there is a lack of studies concerning the systematic characterization of the TPX2 genes family in ***,the identification and portrayal of *** TPX2 genes can provide key targets for molecular manipula-tion in the breeding of cotton fiber *** In this study,TPX2 family genes were classified into two distinct subclasses TPXLs and MAP genes WAVE DAMP-ENED2-LIKE(WDLs)and quite conservative in ***3 was significantly up-regulated in 15 days post anthe-sis fibers of ZRI-015(an upland cotton with longer and stronger fiber).GhWDL3 promotes all stem hairs to become straight when overexpressed in Arabidopsis,which may indirectly regulate cotton fiber cell morphology during fiber *** induced gene silencing(VIGS)results showed that GhWDL3 inhibited fiber cell elongation at fiber development periods through regulating the expression of cell wall related *** These results reveal that GhWDL3 regulated cotton fiber cell elongation and provide crucial information for the further investigation in the regulatory mechanisms/networks of cotton fiber length.

关键词： Upland cotton GhWDL3 Fiber length TPX2 Cytoskeleton Microtubule-associated proteins(MAPs)

Cell membrane-encapsulated nanoparticles for vaccines and immunotherapy

在线全文

学校读者我要写书评

暂无评论

Particuology 2022年第5期20卷 35-42页

作者： Wei Xiang Xue Liu Lili Zhang Chao Liu Gang Liu State Key laboratory of molecular Vaccinology and molecular Diagnostics and Center for molecular Imaging and Translational Medicine School of Public Health.Xiamen UniversityXiamen 361102China State Key laboratory of molecular Vaccinology and molecular Diagnostics and National Institute of Diagnostics and Vaccine Development in Infectious Disease School of Public HealthXiamen UniversityXiamen 361102China ShenZhen Research Institute of Xiamen University.Shenzhen 518057 China

Functionalized nanoparticles are widely used in drug targeted delivery and immune microenvironment *** a new type of biomaterial,cell-derived carriers are receiving considerable attention in clinical translation owing to their desirable biocompatibility and lower ***,cell membrane-encapsulated nanoparticles can achieve specific biological effects from the benefit of their parent cell *** this review,we introduce the cell membrane coating technologies that are under extensive investigation,we also summarize various applications that have been developed for immunotherapy,and discuss the prospects in this *** further understanding and integration with advanced biotechnology methods,it is believed that the continuing development of cell membrane-encapsulated nanoparticles will achieve promotion in clinical application.

关键词： Cell membrane Nanoparticle Vaccine Immunotherapy

Prohibitin Protein Expression During Spermatogenesis in the Large Yellow Croaker, Larimichthys crocea

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Ocean University of China 2022年第5期21卷 1251-1264页

作者： LIN Chenwen GAO Xinming NI Jie ZHANG Shengshuo LIU Cheng LUO Shengyu DU Chen ZHENG Xuebin HOU Congcong TANG Daojun ZHANG Chundan ZHU Junquan Key laboratory of Applied Marine biotechnology by the Ministry of Education School of Marine SciencesNingbo UniversityNingbo 315211China

Mitochondria are important for animals’fertility,and their morphologies and functions during spermatogenesis are un-der ***,the molecular mechanism that regulates the mitochondrial dynamic during spermatogenesis is still *** this study,the cytological features of spermatogenesis were investigated in Larimichthys *** addition,the structure and function of prohibitin(PHB),which is associated with mitochondrial structure and dynamic,was also *** full-length cDNA and protein(Lc-PHB)from the *** phb gene(Lc-phb)contained 1625 base pairs and 271 amino acids,***-PHB had a conserved primary structure that resulted in a transmembrane,SPFH(the analogous region of proteins stomat-ins,prohibitins,flotillins and HflK/C),and coiled-coil *** was detected at high levels in the muscle,liver,and heart,and at intermediate levels in the testis,gill,and ***-phb mRNA expression was detected in spermatogenic cells by fluorescence in situ *** immunofluorescence assay revealed that PHB protein was localized in the mitochondria during ***,PHB expression was detected in the perinuclear cytoplasm of spermatogonia,spermatocytes,and spermatids in the early developmental stage,and mainly localized on one side of the nuclei in the cytoplasm of spermatids in a middle developmental stage,and finally on the sperm *** blotting showed that PHB was located in the extracted mitochondria protein fraction but not in the cytoplasm protein fraction of ***,these results indicated that PHB was expressed in the mitochondria dur-ing *** addition,the study explained the mitochondrial dynamic during fish spermatogenesis and proposed a possi-ble relationship among PHB,spermatogenesis,and male fertility.

关键词： spermatogenesis ultrastructure prohibitin expression pattern subcellular localization

Ring-opening Polymerization of 2-Oxabicyclo[2.2.2]octan-3-one and the Influence of Stereochemistry on the Thermal Properties of the Polyesters

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Polymer Science 2022年第10期40卷 1173-1182,I0008页

作者： Tong Zhou Yu-Ting Guo Fu-Sheng Du Zi-Chen Li Beijing National laboratory for molecular Sciences(BNLMS) Key Laboratory of Polym.Chem.&Physics of Ministry of EducationDepartment of Polymer Science&EngineeringCollege of Chemistry and Molecular EngineeringCenter for Soft Matter Science and EngineeringPeking UniversityBeijing 100871China

Polyesters with cyclic structures in the main chain typically possess superior mechanical and thermal properties together with chemical ***-opening polymerization(ROP)of bridged or fused bicyclic lactones is a simple,and in most cases controlled method to synthesize polyesters with alicyclic moieties in the polymer *** stereochemistry of the alicyclic structures has a great effect on the polymer properties,which can be regulated by varying the polymerization ***,we report a systematic investigation on the ROP of 2-oxabicyclo[2.2.2]octan-3-one([2.2.2]VL)under different *** initiated byn-butyl lithium(n-BuLi)or catalyzed by trifluoromethanesulfonic acid(TfOH)in the presence of benzyl alcohol,P[2.2.2]VLs containing all cis^(-1),4 disubstituted cyclohexane ring were ***,P[2.2.2]VLs initiated by sodium methoxide(MeONa)or catalyzed by organic superbase contained both cis and trans isomeric structural *** cis to trans transformation mechanism under these conditions was manifested,and the effect of stereochemical variations on the properties ofP[2.2.2]VLwas *** stereoregularP[2.2.2]VLs,bothcisandtrans,exhibited higher crystallinity and melting temperatures(Tm)than those of the stereoirregular ***,the degradation of P[2.2.2]VL with acid at high temperature could recover 3-cyclohexenecarboxylic acid.

关键词： 2-Oxabicyclo[2.2.2]octan-3-one Ring-opening polymerization Stereochemistry Polyester Thermal properties

Fusarium-produced vitamin B6 promotes the evasion of soybean resistance by Phytophthora sojae

在线全文

学校读者我要写书评

暂无评论

植物学报:英文版 2023年第9期65卷 2204-2217页

作者： Shuchen Wang Xiaoyi Zhang Zhichao Zhang Yun Chen Qing Tian Dandan Zeng Miao Xu Yan Wang Suomeng Dong Zhonghua Ma Yuanchao Wang Xiaobo Zheng Wenwu Ye Department of Plant Pathology Nanjing Agricultural UniversityNanjing 210095China Key laboratory of Plant Immunity Nanjing Agricultural UniversityNanjing 210095China Key laboratory of Soybean Disease and Pest Control(Ministry of Agriculture and Rural Affairs) Nanjing Agricultural UniversityNanjing 210095China Key laboratory of molecular Biology of Crop Pathogens and Insects Institute of BiotechnologyZhejiang UniversityHangzhou 310058China

Plants can be infected by multiple pathogens concurrently in natural systems. However,pathogen–pathogen interactions have rarely been studied. In addition to the oomycete Phytophthora sojae, fungi such as Fusarium spp. also cause soybean root rot. In a 3-year field investigation, we discovered that P. sojae and Fusarium spp. frequently coexisted in diseased soybean roots. Out of 336 P. sojae–soybean–Fusarium combinations,more than 80% aggravated disease. Different Fusarium species all enhanced P. sojae infection when co-inoculated on soybean. Treatment with Fusarium secreted non-proteinaceous metabolites had an effect equal to the direct pathogen coinoculation. By screening a Fusarium graminearum mutant library, we identified Fusarium promoting factor of Phytophthora sojae infection 1(Fpp1),encoding a zinc alcohol dehydrogenase. Fpp1 is functionally conserved in Fusarium and contributes to metabolite-mediated infection promotion, in which vitamin B6(VB6) produced by Fusarium is key. Transcriptional and functional analyses revealed that Fpp1 regulates two VB6 metabolism genes, and VB6 suppresses expression of soybean disease resistance-related genes. These results reveal that co-infection with Fusarium promotes loss of P. sojae resistance in soybean, information that will inform the sustainable use of diseaseresistant crop varieties and provide new strategies to control soybean root rot.

关键词： fungal metabolites pathogen–host interaction Phytophthora sojae soybean root rot vitamin B6

Diploid chromosome-level reference genome and population genomic analyses provide insights into Gypenoside biosynthesis and demographic evolution of Gynostemma pentaphyllum (Cucurbitaceae)

在线全文

学校读者我要写书评

暂无评论

Horticulture Research 2023年第1期10卷 82-95页

作者： Xiao Zhang Yuhe Zhao Yixuan Kou Xiaodan Chen Jia Yang Hao Zhang Zhe Zhao Yuemei Zhao Guifang Zhao Zhonghu Li Key laboratory of Resource Biology and biotechnology in Western China(Ministry of Education) College of Life SciencesNorthwest UniversityXi’anShaanxi710069China laboratory of Subtropical biodiversity Jiangxi Agricultural UniversityNanchang330045China College of Life Sciences Shanxi Normal UniversityTaiyuanShanxi030012China College of Life Sciences Sun Yat-sen UniversityGuangzhouGuangdong510275China School of Biological Sciences Guizhou Education UniversityGuiyangGuizhou550018China

Gynostemma pentaphyllum(Thunb.)Makino is a perennial creeping herbaceous plant in the family Cucurbitaceae,which has great medicinal value and commercial potential,but urgent conservation efforts are needed due to the gradual decreases and fragmented distribution of its wild ***,we report the high-quality diploid chromosome-level genome of *** obtained using a combination of next-generation sequencing short reads,Nanopore long reads,and Hi-C sequencing *** genome is anchored to 11 pseudo-chromosomes with a total size of 608.95 Mb and 26588 predicted *** genomic analyses indicate that *** is estimated to have diverged from Momordica charantia 60.7 million years ago,with no recent whole-genome duplication *** population analyses based on genotyping-by-sequencing and ecological niche analyses indicated low genetic diversity but a strong population structure within the species,which could classify 32 *** populations into three geographical groups shaped jointly by geographic and climate ***,comparative transcriptome analyses showed that the genes encoding enzyme involved in gypenoside biosynthesis had higher expression levels in the leaves and ***,the findings obtained in this study provide an effective molecular basis for further studies of demographic genetics,ecological adaption,and systematic evolution in Cucurbitaceae species,as well as contributing to molecular breeding,and the biosynthesis and biotransformation of gypenoside.

关键词： evolution jointly breeding