检索结果-南通市图书馆

A larval specific OBP able to bind the major female sex pheromone component in Spodoptera exigua(Hübner)

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative agriculture 2015年第7期14卷 1356-1366页

作者： JIN Rong LIU Nai-yong LIU Yan DONG Shuang-lin Key Laboratory of Integrated Management of crop Diseases and Pests Ministry of Education/College of Plant Protection Nanjing Agricultural University

Odorant binding proteins （OBPs） in insects are postulated to solubilize and transport the hydrophobic odorants across the hydrophilic antennal lymph to the olfactory receptors （ORs） located on the dendrite membrane of the sensory neurons. OBPs in adult insects have been intensively reported, but those in larvae are rarely addressed. In our study, a full-length OBP cDNA, namely SexiOBP13, was cloned by RT-PCR and RACE strategy from the heads of Spodoptera exigua larvae. The quantitative real-time PCR （qPCR） measurement indicated that SexiOBP13 was highly expressed in larval head, but very low in other parts of larva and was not detected in any tissues of adult. The binding affinities of SexiOBP13 to plant volatiles and female sex pheromone components were measured by competitive binding assays. Interestingly, SexiOBP13 displayed a high binding affinity （Ki=3.82 IJmol L-1） to Z9,E12-14：Ac, the major sex pheromone component of S. exigua, while low affinities to the tested host plant volatiles （Ki〉27 μmol L-l）. The behavioral tests further confirmed that Z9,E12-14：Ac was indeed active to elicit the behavioral activity of the third instar larvae of S. exigua. Taken together, our results suggest that SexiOBP13 may play a role in reception of female sex pheromone in S. exigua larvae. The ecological significance of the larvae preference to the adult female sex pheromone was discussed.

关键词： odorant binding protein female sex pheromone larval specificity binding affinity behavioral response

Validation of Reference Genes for Quantitative Real-Time PCR in Laodelphax striatellus

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative agriculture 2014年第4期13卷 811-818页

作者： HE Xiu-ting LIU Cheng-cheng LI Zhao-qun ZHANG Zan LI Guo-qing LI Fei DONG Shuang-lin Key Laboratory of Integrated Management of crop Diseases and Pests Ministry of Education/College of Plant ProtectionNanjing Agricultural University

The normalization of quantitative real-time PCR （qPCR） is important to obtain accurate gene expression data, and the most common method for qPCR normalization is to use reference genes. However, reference genes can be regulated under different conditions, qPCR has recently been used for gene expression study in Laodelphax striatellus, but there is no study on validation of the reference genes. In this study, five new housekeeping genes （LstrTUB1, LstrTUB2, LstrTUB3, LstrARF and LstrRPL9） in L. striatellus were cloned and deposited in the GenBank with accession numbers of JF728809, JF728810, JF728811, JF728807 and JF728806, respectively. Furthermore, mRNA expressions of the five genes and β-actin were measured by qPCR with insect samples of different instar at nymph stage, and the expression stabilities were determined by the software geNorm and NormFinder. As a result, ARF and RPL9 were consistently more stable than β-actin, while three TUB genes were less stable than β-actin. To determine the optimal number of reference genes used in qPCR, a pairwise variations analysis by geNorm indicated that two references ARF and RPL9 were required to obtain the accurate quantification. These results were fiarther confirmed by the validation qPCR experiment with chitinase gene as the target gene, in which the standard error of the mRNA quantification by using binary reference ARF-RPL9 was much lower than those by ARF, RPL9 or β-actin alone. Taken together, our study suggested that the combination of ARF-RPL9 could replace β-actin as the reference genes for qPCR in L. striatellus.

关键词： cDNA cloning housekeeping gene qPCR reference gene small brown planthopper

Identification of three Berberis species as potential alternate hosts for Puccinia striiformis f. sp. tritici in wheat-growing regions of Xinjiang, China

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative agriculture 2019年第12期18卷 2786-2792页

作者： ZHUANG Hua ZHAO Jing HUANG Li-li KANG Zhen-sheng ZHAO Jie State Key Laboratory of crop Stress Biology for Arid Areas College of Plant Protection Northwest A&F University

Since the recent discovery of barberry(Berberis spp.)as an alternate host for the stripe rust pathogen Puccinia striiformis,many Chinese Berberis species have been identified as alternate hosts for *** ***,little is known about Berberis species and their distribution in wheat-growing regions in Xinjiang,China,where stripe rust is *** the largest province or autonomous region,Xinjiang represents a relatively independent epidemic region for wheat stripe rust in *** this study,we con ducted a survey of barberry plants in the main wheat-growi ng areas of *** iden tified three Berberis species,***,*** and ***,and con firmed their roles as potential alternate hosts for *** *** in the laboratory.

关键词： barberry alternate host sexual reproduction stripe rust yellow rust

Molecular Mapping of a Stripe Rust Resistance Gene YrH9020a Transferred from Psathyrostachys huashanica Keng on Wheat Chromosome 6D

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative agriculture 2014年第12期13卷 2577-2583页

作者： LIU Ze-guang YAO Wei-yuan SHEN Xue-xue CHAO Kai-xiang FAN Yu LI Min-zhou WANG Bao-tong LI Qiang JING Jin-xue State Key Laboratory of crop Stress Biology for Arid Areas College of Plant Protection Northwest A&F University

Stripe rust （yellow rust）, caused by Puccinia striiformis f. sp. tritici （Pst）, is one of the most devastating diseases of wheat throughout the world. H9020-1-6-8-3 is a translocation line originally developed from interspeciifc hybridization between wheat line 7182 and Psathyrostachys huashanica Keng and is resistant to most Pst races in China. To identify the resistance gene（s） in the translocation line, H9020-1-6-8-3 was crossed with susceptible cultivar Mingxian 169, and seedlings of the parents, F1, F2, F3, and BC1 generations were tested with prevalent Chinese Pst race CYR32 under controlled greenhouse conditions. The results indicated that there is a single dominant gene, temporarily designated as YrH9020a, conferring resistance to CYR32. The resistance gene was mapped by the F2 population from Mingxian 169/H9020-1-6-8-3. It was linked to six microsatellite markers, including Xbarc196, Xbarc202, Xbarc96, Xgpw4372, Xbarc21, and Xgdm141, lfanked by Xbarc96 and Xbarc202 with at 4.5 and 8.3 cM, respectively. Based on the chromosomal locations of these markers and the test of Chinese Spring （CS） nullitetrasomic and ditelosomic lines, the gene was assigned to chromosome 6D. According to the origin and the chromosomal location, YrH9020a might be a new resistance gene to stripe rust. The lfanking markers linked to YrH9020a could be useful for marker-assisted selection in breeding programs.

关键词： Puccinia striiformis f. sp. tritici Psathyrostachys huashanica Keng resistance gene genetic analysis SSR markers

Efficiency of Different Methods for dsRNA Delivery in Cotton Bollworm(Helicoverpa armigera)

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative agriculture 2014年第1期13卷 115-123页

作者： YANG Jing HAN Zhao-jun Key Laboratory of Integrated Management of crop Diseases and Pests Ministry of Education/College of Plant ProtectionNanjing Agricultural University

RNAi trigged by dsRNA not only facilitates the development of molecular biology, but also initiates a new way for pest control by silence of fatal genes. However, one of the key limitations in pest control is lack of the convenient and efficient method for dsRNA delivery. In this study, different dsRNA delivery methods at their own optimum conditions were evaluated comparatively for their efficiency with Helicoverpa armigera as test animal. It was found that the popular one- time injection of larvae with dsRNA could reduce the pupation rate by 43.0% and enhance larva mortality by 11.7%. One- time ingestion of dsRNA did not result in any significant effect on phenotype. Continuous ingestion of in vitro synthesized dsRNA by refreshing the bait diet every day caused 40.4% decrease in successful pupation and 10.0% increase in larval mortality, which was similar as one-time injection. The most efficient method was found to be the continuous ingestion of the bacteria containing dsRNA expressed, which reduced the rate of pupation by 68.7% and enhanced the larval mortality by 34.1%. Further analysis found that dsRNA was degraded faster in midgut juice than in hemolymph. However, the cell of bacteria could protect dsRNA and delay the degradation in the midgut juice of H. armigera. These results throw light on the application of dsRNA in pest management with proper ways.

关键词： Helicoverpa armigera dsRNA delivery methods RNAi effect pest control

Transcriptome Analysis of oserf922 Mutants Reveals New Insights into Rice Blast Resistance

在线全文

学校读者我要写书评

暂无评论

Rice science 2023年第5期30卷 374-378,I0013-I0016页

作者： QIN Mengchao TAO Hui SHI Xuetao ZHANG Chongyang HE Feng WANG Min LIU Zheng WANG Jisong ZHANG Rongxue WANG Shutong WANG Guoliang NING Yuese WANG Ruyi College of Plant protection Hebei Agricultural UniversityBaoding 071000China State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193China Tianjin Key Laboratory of crop Genetics and Breeding Tianjin Academy of Agricultural SciencesTianjin 300384China Department of Plant Pathology the Ohio State UniversityColumbusOH 43210USA

Rice blast disease,caused by fungal Magnaporthe oryzae,severely threatens food *** susceptibility(S) genes have emerged to support pathogenesis,and disruption of S gene usually confers increased resistance to multiple pathogen *** ethylene response factor gene Os ERF922 is a potential S gene in blast ***,how Os ERF922 negatively regulates resistance against *** is still unknown.

关键词： resistance susceptibility emerged

Identification of a major QTL for Hessian fly resistance in wheat cultivar‘Chokwang’

在线全文

学校读者我要写书评

暂无评论

The crop Journal 2022年第3期10卷 775-782页

作者： Lirong Zhang Yunfeng Xu Ming-Shun Chen Zhenqi Su Yang Liu Yuzhou Xu Guixiao La Guihua Bai Department of Plant Pathology College of Plant ProtectionHebei Agricultural UniversityBaoding 071001HebeiChina Department of agronomy Kansas State UniversityManhattanKS 66506USA Technological Innovation Center for Biological Control of crop Diseases and Insect Pests of Hebei Province Baoding 071001HebeiChina Hard Winter Wheat Genetics Research Unit USDA-ARSManhattanKS 66506USA State Key Laboratory for agrobiotechnology and Key Laboratory of crop Heterosis and Utilization(MOE)and Beijing Key Laboratory of crop Genetic Improvement China Agricultural UniversityBeijing 100193China Institute of South Subtropical crops Chinese Academy of Tropical Agricultural SciencesZhanjiang 524013GuangdongChina Industrial crop Research Institute Henan Academy of Agricultural SciencesZhengzhou 450002HenanChina

The Hessian fly(HF,Mayetiola destructor)is one of the destructive pests of wheat(Triticum aestivum L.)*** cultivars can effectively minimize wheat damage due to this insect *** identify new quantitative trait loci(QTL)for HF resistance,a population of recombinant inbred lines(RILs)was developed from a cross between the HF-resistant wheat cultivar‘Chokwang’and susceptible wheat‘Ning 7840’,and phenotyped for responses to HF attack.A linkage map was constructed using 1147 single nucleotide polymorphism(SNP)markers generated from genotyping-by-sequencing(GBS).One major QTL,***-6 BS,for HF-resistance was identified on chromosome arm 6 BS,which explained up to84.0%of the phenotypic variation and was contributed by *** RILs showed recombination in the candidate interval of ***-6 BS,which delimited ***-6 BS to a 4.75 Mb physical interval between 6,028,601 bp and 10,779,424 bp on chromosome arm 6 BS of IWGSC Chinese Spring reference genome Ref Seq *** GBS-SNPs in the candidate interval were converted into Kompetitive allele specific polymerase chain reaction(KASP)*** of them,KASP-6 B112698 and KASP-6 B7901215,were validated in a *** wheat ***-6 B112698 was nearly diagnostic,thus can be used to screen ***-6 BS and pyramid it with other resistance genes in breeding programs.

关键词： Wheat Hessian fly resistance Mayetiola destructor KASP GBS

Effects of imidacloprid and thiamethoxam as seed treatments on the early seedling characteristics and aphid-resistance of oilseed rape

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative agriculture 2015年第12期14卷 2581-2589页

作者： HUANG Liang ZHAO Chun-lin HUANG Fang BAI Run-e Lü Yao-bin YAN Feng-ming HAO Zhong-ping School of agricultural and Food Science Zhejiang A & F University Institute of Plant protection and Microbiology Zhejiang Academy of Agricultural Sciences College of Plant protection Henan Agricultural University Key Laboratory of crop Quality Improvement of Anhui Province Crop Research Institute Anhui Academy of Agricultural Sciences

Seed treatments with the neonicotinoid insecticides imidacloprid and thiamethoxam were evaluated to determine whether the chemicals at effective concentrations for aphid control would influence the germination and early growth of oilseed rape,Brassica *** with imidacloprid or thiamethoxam did not affect the cumulative germination rate,but significantly inhibited establishment potential by suppressing root system development in the cotyledon ***,these alterations in seedling development in the thiamethoxam-treated seeds appeared not to be detrimental as leaves developed;in contrast,for the seedlings with imidacloprid as seed treatment agent,a significantly decreased shoot/root ratio was still evident at the late two-leaf *** two leaves developed,chlorophyll content per leaf in the thiamethoxam treatment was significantly higher than that of the control,while chlorophyll content per leaf in the imidacloprid treatment remained close to that in the *** other parameters,i.e.,height,leaf area,weight of stem,leaf or root,and other growth indexes,between the treatments and the control showed no significant ***,it was found that storage time of the treated seeds had a significant effect on cumulative germination *** 30 d before planting significantly reduced germination relative to that of the *** of the plants treated with neonicotinoids were shown to have significant anti-aphid characteristics that persisted until the end of the trial.

关键词： oilseed rape seed coating agent imidacloprid thiamethoxam seedling growth germination aphid

Genetic Analysis and Molecular Mapping of a Stripe Rust Resistance Gene YrH9014 in Wheat Line H9014-14-4-6-1

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative agriculture 2013年第4期12卷 638-645页

作者： MA Dong-fang HOU Lu TANG Ming-shuang WANG Hai-ge LI Qiang JING Jin-xue State Key Laboratory of crop Stress Biology for Arid Areas College of Plant Protection Northwest A&F University

Stripe rust, caused by Puccinia striiformis f. sp. tritici （Pst）, is one of the most widespread and destructive wheat diseases in many wheat-growing regions of the world. The winter wheat translocation line H9014-14-4-6-1 has all stage resistance. To identify stripe rust resistance genes, the segregating populations were developed from the cross between H9014-14-4-6-1 and Mingxian 169 （a wheat cultivar susceptible to all Pst races identified in China）. The seedlings of the parents and F1 plants, Fz, F3 and BC1 generations were tested with Pst races under controlled greenhouse conditions. Two genes for resistance to stripe rust were identified, one dominant gene conferred resistance to SUN11-4, temporarily designated YrH9014 and the other recessive gene conferred resistance to CYR33. The bulked segregant analysis and simple sequence repeat （SSR） markers were used to identify polymorphic markers associated with YrH9014. Seven polymorphic SSR markers were used to genotype the F2 population inoculated with SUN11-4. A linkage map was constructed according to the genotypes of seven SSR markers and resistance gene. The molecular map spanned 24.3 cM, and the genetic distance of the two closest markers Xbarc13 and Xbarc55 to gene locus was 1.4 and 3.6 cM, respectively. Based on the position of SSR marker, the resistance gene YrH9014 was located on chromosome arm 2BS. Amplification of a set of nulli-tetrasomic Chinese Spring lines with SSR marker Xbarc13 indicated that YrH9014 was located on chromosome 2B. Based on chromosomal location, the reaction patterns and pedigree analysis, YrH9014 should be a novel resistance gene to stripe rust. This new gene and flanking markers got from this study should be useful for marker-assisted selection （MAS） in breeding programs for stripe rust.

关键词： wheat cultivar Puccinia striiformis f. sp. tritici resistance genes molecular mapping Psathyrostachyshuashanica Keng