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Sorption and desorption of pymetrozine on six Chinese soils

Frontiers of Environmental Science & Engineering 2016年第1期10卷 1-10页

作者： Mingxing GAO Yingying LI Hong YANG Yucheng GU Jiangsu Key Laboratory of Pesticide Science College of Sciences Nanjing Agricultural University Nanjing 210095 China syngenta Nantong crop protection Co. Ltd. Nantong Economic & Technological Development Area Nantong 226009 China syngenta Jealott's Hill International Research Centre Bracknell Berkshire RG42 6EY UK

Pymetrozine is a selective insecticide with a unique chemical structure and mode to control hemipteran and homopteran. While pymetrozine has brought great benefits to crop production by killing insects, its residues in soil may have a detrimental effect on environment. Therefore, it is of great importance to investigate its behaviors in soil. In this study, the sorption and desorption of pymetrozine on six Chinese soils were investigated using a batch equilibrium approach to understand its mobile behavior in the soils. Both sorption and desorption isotherms ofpymetrozine were in good agreement with the Freundlich model. The sorption coefficient KF varied between 3.37 and 58.32 mL. g-1 and the sorption isotherms were nonlinear, with 1/n ranging from 0.57 to 0.91. A regression equation was proposed to predict the solption of pymetrozine on six different soil samples： log KF = 4.3708 - 4.5709 × log （pH in 0.01mol·L-1 CaC12）＋ 0.4700 × log OC% ＋ 0.0057 × sand （%）＋ 0.0022 × CEC（clay）, with R2 = 0.9982. The organic carbon content of soil positively affected the sorption ofpymetrozine, but soil pH had a negative effect on the sorption. Additionally, effects of CaC12 concentration, soil to solution ratio and pesticide form were investigated. The sorption was promoted with an increase in soil to solution ratio and a decrease in CaC12 concentration. The possible variation of the five formulated products of pymetrozine was also investigated.

关键词： pymetrozine sorption desorption soil

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Variation in cis-regulation of a NAC transcription factor contributes to drought tolerance in wheat

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Molecular Plant 2022年第2期15卷 276-292页

作者： Hude Mao Shumin Li Bin Chen Chao Jian Fangming Mei Yifang Zhang Fangfang Li Nan Chen Tian Li Linying Du Li Ding Zhongxue Wang Xinxiu Cheng Xiaojing Wang Zhensheng Kang State Key Laboratory of crop Stress Biology for Arid Areas and College of Plant protection Northwest A&F UniversityYanglingShaanxi 712100China Pioneering Innovation Center for Wheat Stress Tolerance Improvement State Key Laboratory of Crop Stress Biology for Arid AreasYanglingShaanxi 712100China Key Laboratory of crop Gene Resources and Germplasm Enhancement Ministry of AgricultureInstitute of Crop ScienceChinese Academy of Agricultural SciencesBeijing 100081China State Key Laboratory of crop Stress Biology for Arid Areas and College of Life Science Northwest A&F UniversityYanglingShaanxi 712100China Yangling Seed Industry Innovation Center YanglingShaanxi 712100China

Drought is a major environmental factor limiting wheat production worldwide,and developing drought-tolerant cultivars is a central challenge for wheat breeders ***,it is important to identify genetic components determining drought tolerance in *** this study,we identified a wheat NAC gene(TaNAC071-A)that is tightly associated with drought tolerance by a genome-wide association *** of TaNAC071-A in wheat attenuated plant drought tolerance,whereas its overexpression significantly enhanced drought tolerance through improved water-use efficiency and increased expression of stress-responsive *** heightened water-saving mechanism mitigated the yield loss caused by water *** candidate gene association analysis showed that a 108-bp insertion in the promoter of TaNAC071-A alters its expression level and contributes to variation in drought tolerance among wheat *** insertion contains two MYB cis-regulatory elements(CREs)that can be directly bound by the MYB transcription activator,TaMYBL1,thereby leading to increased TaNAC071-A expression and plant drought ***,introgression of this 108-bp insertion allele,TaNAC071-AIn-693,into drought-sensitive cultivars could improve their drought tolerance,demonstrating that it is a valuable genetic resource for wheat *** together,our findings highlight a major breakthrough in determining the genetic basis underlying phenotypic variation in wheat drought tolerance and showcase the potential of exploiting CRE-containing indels for improving important agronomical traits.

关键词： wheat(Triticum aestivum) drought tolerance GWAS TaNAC071-A cis-regulatory element transcrip-tional regulation

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Two NIS1-like proteins from apple canker pathogen(Valsa mali)play distinct roles in plant recognition and pathogen virulence

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Stress Biology 2022年第1期2卷 606-620页

作者： Jiajun Nie Wenjing Zhou Yonghui Lin Zhaoyang Liu Zhiyuan Yin Lili Huang State Key Laboratory of crop Stress Biology for Arid Areas College of Plant ProtectionNorthwest A&F University3 Taicheng RoadYangling 712100ShaanxiChina College of Plant protection China Agricultural UniversityBeijing 100193China

Conserved effectors produced by phytopathogens play critical roles in plant-microbe ***1-like proteins represent a newly identified family of effectors distributed in multiple fungal ***,their biological functions in a majority of pathogenic fungi remain largely elusive and require further *** this study,we characterized two NIS1-like proteins VmNIS1 and VmNIS2 from Valsa mali,the causal agent of apple Valsa *** of these two proteins were predicted to be *** agroinfiltration,we found that VmNIS1 induced intense cell death,whereas VmNIS2 suppressed INF1 elicitin-triggered cell death in Nicotiana *** of *** with VmNIS1 recombinant protein produced by Escherichia coli activated a series of immune responses and enhanced plant disease resistance against Phytophthora *** contrast,VmNIS2 suppressed plant immune responses and promoted *** infection when transiently expressed in *** VmNIS1 and VmNIS2 were shown to be highly induced at late stage of *** *** individually knocking out of these two genes in ***,however,only VmNIS2 was shown to be required for pathogen virulence as well as tolerance to oxidative ***,we further showed that C-terminal extension of VmNIS1 was essential for plant recognition and VmNIS2 may escape plant detection via sequence *** data collectively indicate that VmNIS1 and VmNIS2 play distinct roles in plant recognition and pathogen virulence,which provided new insights into the function of NIS1-like proteins in plant-microbe interactions.

关键词： Valsa mali Effector protein Cell death Plant immunity Virulence

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Effects of nitrogen fertilizer on root-shoot characteristics,grain yield and nitrogen use efficiency of different maize varieties in North China Plain

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International Journal of agricultural and Biological Engineering 2022年第5期15卷 132-140页

作者： Xiangling Li Liu Pan Rui Wang Yiping Zuo Jian Wang Jinling Han Yuechen Zhang Baozhong Yin College of agronomy and Biotechnology Hebei Normal University of Science&Technology/Hebei Key Laboratory of Crop Stress BiologyQinhuangdao 066004HebeiChina College of agronomy Hebei Agricultural University/State Key Laboratory of North China Crop Improvement and RegulationBaoding 071001HebeiChina College of Plant protection Hebei Agricultural University/State Key Laboratory of North China Crop Improvement and RegulationBaoding 071001HebeiChina

Excessive nitrogen(N)fertilizer application in crop production has resulted in a series of environmental issues in North China Plain(NCP).Breeding N-efficient maize varieties is one of the effective strategies to improve the N use efficiency of maize and reduce environmental *** study designed an experiment for two maize varieties(‘Xian-Yu 335’[XY335]and‘Hua-Nong 138’[HN138])with three N-level treatments(0 kg/hm^(2),180 kg/hm^(2),300 kg/hm^(2),i.e.N0,N180,N300).The aim of the study was to determine the response characteristics of maize canopy structure,root morphology,and anatomical structure,as well as N uptake and utilization of N *** showed that grain yield,N use efficiency,and N harvest index of XY335 were higher 5.20%-13.68%,11.86%-19.11%,and 6.07%-3.33%than those of HN138,*** with HN138,XY335 had a higher leaf area index and photosynthetic nitrogen-use *** root length,root length density,root absorption area,root absorption area,and root active absorption area of XY335 in each period were higher than that of *** proportion of RCA of XY335 was significantly higher than that of HN138,which was 14.64%higher on *** the future breeding process,the high N and low N double-high-efficiency varieties should pay more attention to the improvement of root cortex aeration tissue.

关键词： maize root nitrogen accumulation nitrogen use efficiency yield

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Diagnosis and characterization of the ribosomal DNA-ITS of potato rot nematode(Ditylenchus destructor) populations from Chinese medicinal herbs

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Journal of Integrative agriculture 2023年第6期22卷 1763-1781页

作者： NI Chun-hui HAN Bian LIU Yong-gang Maria MUNAWAR LIU Shi-ming LI Wen-hao SHI Ming-ming LI Hui-xia PENG De-liang College of Plant protection Gansu Agricultural University/Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu ProvinceLanzhou 730070P.R.China Institute of Plant protection Gansu Academy of Agricultural SciencesLanzhou 730070P.R.China Department of Biological Sciences University of LethbridgeLethbridge 4401Canada State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China

The potato rot nematode(Ditylenchus destructor) is a very economically important nematode in agronomic and horticultural plants worldwide. In this study, 43 populations of D. destructor were collected from different hosts across China, including 37 populations from Chinese herbal medicine plants. Obtained sequences of ITS-rDNA and D2–D3of 28S-rDNA genes of D. destructor were compared and analyzed. Nine types of significant length variations in ITS sequences were observed among all populations. The differences in ITS1 length were mainly caused by the presence of repetitive elements with substantial base substitutions. Reconstructions of ITS1 secondary structures showed that the minisatellites formed a stem structure. Ten haplotypes were observed in all populations based on mutations and variations of helix H9. Among them, 3 known haplotypes(A–C) were found in 7 populations isolated from potato,sweet potato, and Codonopsis pilosula, and 7 unique haplotypes were found in other 36 populations collected from C. pilosula and Angelica sinensis compared with 7 haplotypes(A–G) according to Subbotin' system. These unique haplotypes were different from haplotypes A–G, and we named them as haplotypes H–N. The present results showed that a total of 14 haplotypes(A–N) of ITS-rDNA have been found in D. destructor. Phylogenetic analyses of ITSrDNA and D2–D3 showed that all populations of D. destructor were clustered into two major clades: one clade only containing haplotype A from sweet potato and the other containing haplotypes B–N from other plants. For further verification, PCR-ITS-RFLP profiles were conducted on 7 new haplotypes. Collectively, our study suggests that D. destructor populations on Chinese medicinal materials are very different from those on other hosts and this work provides a paradigm for relevant researches.

关键词： Ditylenchus destructor minisatellites ITS-RFLP phylogeny RNA secondary structure

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THE ROLE OF PHOSPHORUS IN crop protection:EXPERIMENTAL AND COMMERCIAL DISEASE AND WEED CONTROL agENTS

THE ROLE OF PHOSPHORUS IN CROP PROTECTION:EXPERIMENTAL AND C...

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第17届国际磷化学大会

作者： Roger G.Hall syngenta crop protection Switzerland

＜正＞Although the importance of Organophosphate insecticides is steadily declining, Phosphorus still plays an important role in crop protection. Glyphosate continues to dominate the herbicide market,

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In vitro and in silico studies of salicylic acid on systemic induced resistance against bacterial leaf blight disease and enhancement of crop yield

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Journal of Integrative agriculture 2023年第1期22卷 170-184页

作者： Wannaporn THEPBANDIT Narendra Kumar PAPATHOTI Jayasimha Rayulu DADDAM Nguyen Huy HOANG Toan LE THANH Chanon SAENGCHAN Kumrai BUENSANTEAI School of crop Production Technology Suranaree University of TechnologyNakhon Ratchasima 30000Thailand Department of Animal Science Agriculture Research OrganizationRishon Lezion 7505101Israel Department of Plant protection Can Tho UniversityCan Tho 900000Vietnam

Salicylic acid(SA)is an effective elicitor to promote plant defenses and *** study aimed to investigate rice(Oryza sativa L.)*** Dawk Mali 105 treated with salicylic acid(SA)-Ricemate as an enhanced plant protection mechanism against bacterial leaf blight(BLB)disease caused by Xanthomonas oryzae ***(Xoo).Results indicated that the use of SA-Ricemate as a foliar spray at concentrations of more than 100 mg L^(-1)can reduce the severity of BLB disease by 71%.SA-Ricemate treatment also increased the hydrogen peroxide(H_(2)O_(2))content of rice leaf tissues over untreated samples by 39–61%.Malondialdehyde(MDA)in rice leaves treated with SA-Ricemate also showed an increase of 50–65%when comparing to non-treated *** differential development of these defense compounds was faster and distinct when the SA-Ricemate-treated rice was infected with Xoo,indicating plant-induced ***,SA-Ricemate elicitor at a concentration of 50–250 mg L^(-1)was correlated with a substantial increase in the accumulation of total chlorophyll content at 2.53–2.73 mg g^(-1)of fresh weight which suggests that plant growth is activated by *** catalase-and aldehyde dehydrogenase-binding sites were searched for using the CASTp server,and the findings were compared to the *** was used to design and optimize SA,which was then docked to the catalase and aldehyde dehydrogenase-binding domains of the enzymes using the GOLD 3.0.1 *** is shown in several docked conformations with the enzymes catalase and aldehyde *** three catalase amino acids(GLN7,VAL27,and GLU38)were discovered to be involved in the creation of a strong hydrogen bond with SA when SA was *** this mechanism,the aldehyde dehydrogenase amino acids LYS5,HIS6,and ASP2 were all implicated,and these amino acids created strong hydrogen bonds with *** field conditions,SA-Ricemate significantly reduced disease severity by 78%and the total grain yield was si

关键词： bacterial leaf blight crop yield in vitro in silico rice defense salicylic acid

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Puccinia triticina effector protein Pt_21 interacts with wheat thaumatin-like protein TaTLP1 to inhibit its antifungal activity and suppress wheat apoplast immunity

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作物学报:英文版 2023年第5期11卷 1431-1440页

作者： Fei Wang Songsong Shen Zhongchi Cui Shitao Yuan Ping Qu Hui Jia Linshuo Meng Xiaoyu Hao Daqun Liu Lisong Ma Haiyan Wang College of Plant protection Hebei Agricultural University/Technological Innovation Center for Biological Control of Crop Diseases and Insect Pests of Hebei ProvinceBaoding 071001HebeiChina Center of agriculture Through Science and Education Hebei Agricultural UniversityBaoding 071001HebeiChina The State Key Laboratory of North China crop Improvement and Regulation Hebei Agricultural UniversityBaoding 071001HebeiChina

Puccinia triticina(Pt), as the causal agent of wheat leaf rust, employs a plethora of effector proteins to modulate wheat immunity for successful colonization. Understanding the molecular mechanisms underlying Pt effector-mediated wheat susceptibility remains largely unexplored. In this study, an effector Pt_21 was identified to interact with the apoplast-localized wheat thaumatin-like protein TaTLP1 using a yeast two-hybrid assay and the Pt_21-TaTLP1 interaction was characterized. The interaction between Pt_21 and TaTLP1 was validated by in vivo co-immunoprecipitation assay. A TaTLP1 variant,TaTLP1C71A, that was identified by the site-directed mutagenesis failed to interact with Pt_21. Pt_21was able to suppress Bax-mediated cell death in leaves of Nicotiana benthamiana and inhibit TaTLP1-mediated antifungal activity. Furthermore, infiltration of recombinant protein Pt_21 into leaves of transgenic wheat line overexpressing TaTLP1 enhanced the disease development of leaf rust compared to that in wild-type leaves. These findings demonstrate that Pt_21 suppresses host defense response by directly targeting wheat TaTLP1 and inhibiting its antifungal activity, which broadens our understanding of the molecular mechanisms underlying Pt effector-mediated susceptibility in wheat.

关键词： Wheat Puccinia triticina Effector Thaumatin-like protein Anitifungal activity

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Weed genomics:yielding insights into the genetics of weedy traits for crop improvement

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aBIOTECH 2023年第1期4卷 20-30页

作者： Yujie Huang Dongya Wu Zhaofeng Huang Xiangyu Li Aldo Merotto Jr Lianyang Bai Longjiang Fan Institute of crop Science and Institute of Bioinformatics Zhejiang UniversityHangzhou 310058China Institute of Plant protection Chinese Academy of Agricultural SciencesBeijing 100193China Department of crop Sciences Agricultural School Federal University of Rio Grande do SulPorto Alegre 91540-000Brazil Hunan Weed Science Key Laboratory Hunan Academy of Agriculture SciencesChangshang 410125China

Weeds cause tremendous economic and ecological damage *** number of genomes established for weed species has sharply increased during the recent decade,with some 26 weed species having been sequenced and de novo genomes *** genomes range from 270 Mb(Barbarea vulgaris)to almost 4.4 Gb(Aegilops tauschii).Importantly,chromosome-level assemblies are now available for 17 of these 26 species,and genomic investigations on weed populations have been conducted in at least 12 *** resulting genomic data have greatly facilitated studies of weed management and biology,especially origin and *** weed genomes have indeed revealed valuable weed-derived genetic materials for crop *** this review,we summarize the recent progress made in weed genomics and provide a perspective for further exploitation in this emerging field.

关键词： Weeds Genome sequencing Population genomics Adaptive traits Evolution

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Plant virology in the 21st century in China:Recent advances and future directions

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Journal of Integrative Plant Biology 2024年第3期66卷 579-622页

作者： Jianguo Wu Yongliang Zhang Fangfang Li Xiaoming Zhang Jian Ye Taiyun Wei Zhenghe Li Xiaorong Tao Feng Cui Xianbing Wang Lili Zhang Fei Yan Shifang Li Yule Liu Dawei Li Xueping Zhou Yi Li State Key Laboratory for Ecological Pest Control of Fujian and Taiwan crops Vector-borne Virus Research CenterCollege of Plant ProtectionFujian Agriculture and Forestry UniversityFuzhou 350002China State Key Laboratory of Plant Environmental Resilience and Ministry of agriculture Key Laboratory of Soil Microbiology College of Biological SciencesChina Agricultural UniversityBeijing 100193China State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193China State Key Laboratory of Integrated Management of Pest Insects and Rodents Institute of ZoologyChinese Academy of SciencesBeijing 100101China CAS Center for Excellence in Biotic Interactions University of Chinese Academy of SciencesBeijing 100049China State Key Laboratory of Plant Genomics Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China State Key Laboratory of Rice Biology Institute of BiotechnologyZhejiang UniversityHangzhou 310058China Department of Plant Pathology The Key Laboratory of Plant ImmunityNanjing Agricultural UniversityNanjing 210095China State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of agro-products Institute of Plant VirologyNingbo UniversityNingbo 315211China MOE Key Laboratory of Bioinformatics Center for Plant BiologySchool of Life SciencesTsinghua UniversityBeijing 100084China State Key Laboratory of Protein and Plant Gene Research School of Life SciencesPeking UniversityBeijing 100871China

Plant viruses are a group of intracellular pathogens that persistently threaten global food *** advances in plant virology have been achieved by Chinese scientists over the last 20 years,including basic research and technologies for preventing and controlling plant viral ***,we review these milestones and advances,including the identification of new crop-infecting viruses,dissection of pathogenic mechanisms of multiple viruses,examination of multilayered interactions among viruses,their host plants,and virus-transmitting arthropod vectors,and in-depth interrogation of plantencoded resistance and susceptibility ***,various plant virus-based vectors have also been successfully developed for gene function studies and target gene expression in *** also recommend future plant virology studies in China.

关键词： China emerging plant viruses plant virology plant resistance/susceptibility to viral infection plant-virus transmission vector interactions plant virus-based vectors viral pathogenesis

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