检索结果-南通市图书馆

Diverse Transcriptional Programs Associated with Environmental Stress and Hormones in the Arabidopsis Receptor-Like Kinase Gene Family

引用

Molecular Plant 2009年第1期2卷 84-107页

作者： Lee Chae Sylvia Sudat Sandrine Dudoit Tong Zhu Sheng Luan Department of Plant and Microbial Biology University of California Berkeley CA 94720 USA Division of Biostatistics School of Public Health University of California Berkeley CA 94720 USA syngenta biotechnology inc. Research Triangle Park NC 27709 USA

The genome ofArabidopsis thaliana encodes more than 600 receptor-like kinase （RLK） genes, by far the dominant class of receptors found in land plants. Although similar to the mammalian receptor tyrosine kinases, plant RLKs are serine/threonine kinases that represent a novel signaling innovation unique to plants and, consequently, an excellent opportunity to understand how extracellular signaling evolved and functions in plants as opposed to animals. RLKs are predicted to be major components of the signaling pathways that allow plants to respond to environmental and developmental conditions. However, breakthroughs in identifying these processes have been limited to only a handful of individual RLKs. Here, we used a syngenta custom Arabidopsis GeneChip array to compile a detailed profile of the tran- scriptional activity of 604 receptor-like kinase genes after exposure to a cross-section of known signaling factors in plants, inc.uding abiotic stresses, biotic stresses, and hormones. In the 68 experiments comprising the study, we found that 582 of the 604 RLK genes displayed a two-fold or greater change in expression to at least one of 12 types of treatments, thereby providing a large body of experimental evidence for targeted functional screens of individual RLK genes. We investigated whether particular subfamilies of RLK genes are responsive to specific types of signals and found that each subfamily displayed broad ranges of expression, as opposed to being targeted towards particular signal classes. Finally, by analyzing the divergence of sequence and gene expression among the RLK subfamilies, we present evidence as to the functional basis for the expansion of the RLKs and how this expansion may have affected conservation and divergences in their function. Taken as a whole, our study represents a preliminary, working model of processes and interactions in which the members of the RLK gene family may be involved, where such information has remained elusive for so many

关键词： Abiotic/environmental stress hormone biology receptors transcriptome analysis disease responses Arabidopsis.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A novel zebrafish vascular injury model for asseessing drug efficancy of Yangxue Qingnao granules

引用

中国药理学与毒理学杂志 2018年第4期32卷 322-323页

作者： Yi-qiao XU Sai-sai BAI Yu-qing FAN Chun-qi LI Hunter biotechnology inc. Hangzhou 310051 China

OBJECTIVE To assess the efficancy of Yangxue Qingnao granules on simvastatininduced vascular injury model in *** Sinc. statins can inhibit vascular development in zebrafish,in this study,we developed a novel animal model using 1 to 3 day post-fertilization larval zebrafish by optimizing the doses and duration of simvastatin *** pro-angiogenic drugs with a variety of mechanisms were tested to validate the newly developed zebrafish *** was treated with different concentration of Yangxue Qingnao granules(62.5,125 and 250 μg·mL-1) for 2 d then tested for the area of subintestinal vein *** Vascular regeneration promoting effect of five pro-angiogenic drugs(calycosin,astragaloside,chlorogenic acid,ferulic acid and Panax Notoginseng Saponins) were 8-48%,24-51%,35-58%,28-75% and 37-69%,*** 62.5,125 and 250 μg·mL-1 Yangxue Qingnao granules group,vascular regeneration promoting effect were 21%(P>0.05),84%(P<0.01) and 53%(P<0.01).CONCLUSION Our results demonstrate that the zebrafish vascular injury model validated in this study could be used for in vivo angiogenesis studies and drug screening and for assessing pro-angiogenic drugs with different *** Qingnao granules could promote the vascular regeneration in zebrafish.

关键词：他汀类药物血管生成药物治疗方法临床分析

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Ubiquitination-mediated protein degradation and modification:an emerging theme in plant-microbe interactions

引用

Cell Research 2006年第5期16卷 413-426页

作者： Li-Rong Zeng~1 Miguel E Vega-Sánchez~1 Tong Zhu~2 Guo-Liang Wang~(1,3)~1Department of Plant Pathology and Plant Molecular Biology and biotechnology Program,The Ohio State University,Columbus,OH 43210,USA:~2syngenta biotechnology inc.Research Triangle Park,NC 27709-2257.USA:~3Rice Genomics Laboratory,Human Agricultural University,Changsha,Human 410128,China Department of Plant Pathology and Plant Molecular Biology and biotechnology Program The Ohio State University Columbus USA syngenta biotechnology inc Research Triangle Park USA Rice Genomics Laboratory Hunan Agricultural University Changsha China

Post-translational modification is central to protein stability and to the naodulation of protein *** types ofprotein modification,such as phosphorylation,methylation,acetylation,myristoylation,glycosylation,and ubiquitina-tion,have been *** them,ubiquitination distinguishes itself from others in that most of the ubiquitinatedproteins are targeted to the 26S proteasome for *** ubiquitin/26S proteasome system constitutes the majorprotein degradation pathway in the *** recent years,the importance of the ubiquitination machinery in the controlof numerous eukaryotic cellular functions has been inc.easingly *** number of E3 ubiquitin ligasesand their substrates,inc.uding a variety of essential cellular regulators have been *** in the past severalyears have revealed that the ubiquitination system is important for a broad range of plant developmental processes andresponses to abiotic and biotic *** review discusses recent advances in the functional analysis of ubiquitina-tion-associated proteins from plants and pathogens that play important roles in plant-microbe interactions.

关键词： Ubiquitination defense response plant-microbe interactions U-box protein Sp111

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Changes in microorganisms and metabolites during long-term fermentation of commercially manufactured anchovy sauce

引用

Food Science and Human Wellness 2024年

作者： Daehyeon Kim Seong-Eun Park Eun-Ju Kim Seung-Ho Seo Kwang-Moon Cho Sun Jae Kwon Hong-Seok Son Department of biotechnology， College of Life Sciences and biotechnology， Korea University AccuGene inc.

This study was performed to investigate the changes in microbial communities and metabolites during the long-term fermentation of commercially manufactured anchovy sauce. Samples of commercial anchovy sauce were collected from large-scale fermentation tanks with fermentation periods of up to 7.87 years. The complex bacterial community was simplified to two genera， Tetragenococcus and Halanaerobium， after approximately 0.55 years of fermentation. Although genera， such as Saccharomyces， Cladosporium， Candida， and Aspergillus， were relatively dominant， no clear pattern was identified in fungal community analysis. The longitudinal metabolite profile demonstrated that approximately half (55.8%) of the metabolites present in anchovy sauce were produced within a year of fermentation due to rapid fermentation. Despite the static microbial community， the contents of several metabolites inc.uding amino acids and biogenic amines changed continuously during the long-term fermentation of anchovy sauce. This study provides novel insights into the changes in microbiota and metabolites in fish sauce produced without any starter inoculation.

关键词：

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

The effects of high temperature level on square Bt protein concen-tration of Bt cotton

引用

Journal of Integrative Agriculture 2015年第10期14卷 1971-1979页

作者： WANG Jun CHEN Yuan YAO Meng-hao LI Yuan WEN Yu-jin CHEN Yuan ZHANG Xiang CHEN De-hua Jiangsu Provinc.al Key Laboratory of Crops Genetics and Physiology Yangzhou University Yangzhou 225009 P.R. China Department of Soil and Crop Sciences Texas A&M University College Station TX77843 USA syngenta biotechnology inc. Carry NC 27519 USA

Higher boll worm survival rates were detected after high temperature presented during square period in Bt cotton. The objective of this study was to investigate the effects of high temperature level on the Bt efficacy of two different types of Bt cotton cultivars at squaring stage. During the 2011 to 2013 cotton growth seasons, high temperature treatments ranged from 34 to 44°C in climate chambers, and field experiments under high temperature weather with various temperature levels were conducted to investigate the effects of the high temperature level on square Bt protein concentration and nitrogen metabolism. The climate chamber experiments showed that the square insecticidal protein contents reduced after 24 h elevated temperature treatments for both cultivars, whereas significant declines of the square insecticidal protein contents were detected at temperature 〉38°C, and only slightly numerical reductions were observed when temperature below 38°C. Similar high temperature responses were also observed at the two field experimental sites in 2013. Correspondingly, high temperature below 38°C seems have little effect on the square amino acid concentrations, soluble protein contents, glutamic-pyruvic transaminase（GPT） and glutamic-oxalacetic transaminase（GOT） activities as well as protease and peptidase activities; however, when the temperature was above 38°C, reduced soluble protein contents, enhanced amino acid concentrations, decreased GPT and GOT activities, bolstered protease and peptidase activities in square were detected. In general, the higher the temperature is（〉38°C）, the larger the changes for the above compound contents and key enzymes activities of the square protein cycle. The findings indicated that the unstable insect resistance of the square was related to high temperature level during square stage.

关键词： Bt cotton square high temperature level stress Bt protein nitrogen metabolism

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Microarray-assisted Marker Development for Molecular Breeding

引用

分子植物育种 2007年第2期5卷 232-233页

作者： C. Chilcott M. Dunn L. Ambroso G. Dace L. Grivet R. Dietrich G. Gay I. Puddephat J. Arbuckle E. Legg J. Salmeron syngenta biotechnology Inc. 3054 Cornwallis Road Research Triangle Park NC 27709 USA

The advantage of being able to investigate the abundance and composition of millions of sequences in parallel has established microarrays as an attractive technology for genome wide transcription and sequence variatio... 详细信息

关键词：分子育种基因多态性标记辅助技术农产品

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Reconstruction of enzymatic activity from split genes encoding glyphosate-tolerant EPSPS protein of Psedomonas fluorescens G2 strain by intein mediated protein complementation

引用

Chinese Science Bulletin 2006年第13期51卷 1652-1654页

作者： DUN Baoqing LU Wei ZHANG Wei PING Shuzhen WANG Xujing CHEN Ming XU Yuquan JIN Dan WANG Jin ZHAO Zhonglin LIANG Aimin HOU Songna XU Ming-Qun LIN Min biotechnology Research Institute Chinese Academy of Agricultural Sciences Beijing 100081 China New England Biolabs Inc. Beverly Massachusetts 01915 USA

A mutagenesis library was constructed using GPS-LS system to insert a random 5 aa into the 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) encoded by aroA gene. Active EPSPS pro- teins were identified by the ability to rescue growth of aroA-deleted mutant ER2799 on M9 minimal media. 12 unique sites, which can tolerate a 5-aa insertion, were identified. In all of the 12 sites, only F295/T296 site was found to split the G2-EPSPS properly by co-transformation of plasmids into E. coli ER2799. The G2-EPSPS gene was then divided into N-termi- nal and C-terminal from F295/T296 site which were fused to the N-terminal and C-terminal of *** intein, respectively, creating two plasmids pMEPS- N295IN and pKEPSc296Ic. Co-transformation of plasmids, pMEPSN295IN and pKEPSc296Ic, rescu- ed growth of ER2799 in M9 minimal media, indicating that the intein splicing domains were bringing the EPSPS fragments together to generate activity. Re- consituted activity of splitted G2-EPSPS enzyme was 4.48 U/mg.

关键词：断裂基因蛋白质再生转基因 EPSPS

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A gain-of-function mutation in Msl10 triggers cell death and wound-induced hyperaccumulation of jasmonic acid in Arabidopsis

引用

Journal of Integrative Plant Biology 2016年第6期58卷 600-609页

作者： Yan Zou Satya Chintamanani Ping He Hirotada Fukushige Liping Yu Meiyu Shao Lihuang Zhu David F.Hildebrand Xiaoyan Tang Jian-Min Zhou School of Life Science and Technology Shanghai Tech University syngenta biotechnology Inc.Slater Department of Biochemistry and Biophysics Institute for Plant Genomics and Biotechnology Texas A&M University Department of Agronomy Agricultural Sciences Center-Noth University of Kentucky State Key Laboratory of Plant Genomics Institute of Genetics and Developmental Biology Chinese Academy of Sciences School of Life Sciences Capital Normal University

Jasmonates（JAs） are rapidly induced after wounding and act as key regulators for wound induced signaling pathway. However, what perceives the wound signal and how that triggers JA biosynthesis remains poorly understood. To identify components involved in Arabidopsis wound and JA signaling pathway, we screened for mutants with abnormal expression of a luciferase reporter, which is under the control of a wound-responsive promoter of an ethylene response factor（ERF） transcription factor gene, RAP2.6（Related to APetala 2.6）.The rea1（RAP2.6 expresser in shoot apex） mutant constitutively expressed the RAP2.6-LUC reporter gene in young *** with the typical JA phenotypes inc.uding shorter petioles,loss of apical dominance, accumulation of anthocyanin pigments and constitutive expression of JA response gene, rea1 plants also displayed cell death and accumulated high levels of JA in response to wounding. The phenotype of rea1 mutant is caused by a gain-of-function mutation in the C-terminus of a mechanosensitive ion channel MscS-like 10（MSL10）. MSL10 is localized in the plasma membrane and is expressed predominantly in root tip, shoot apex and vascular tissues. These results suggest that MSL10 is involved in the wound-triggered early signal transduction pathway and possibly in regulating the positive feedback synthesis of JA.

关键词： Arabidopsis cell death jasmonates mechanosensitive ion channel wound

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Design of ionic liquids as liquid desiccant for an air conditioning system

引用

Green Energy & Environment 2019年第2期4卷 139-145页

作者： Haruki Watanabe Takuro Komura Ryo Matsumoto Kenta Ito Hiroshi Nakayama Toshiki Nokami Toshiyuki Itoh Department of Chemistry and biotechnology Graduate School of Engineering Tottori University Chubu Electric Power Company Inc. Research and Development Division Energy Application Research and Development Center Center for Research on Green Sustainable Chemistry Tottori University

Suitable control of the humidity can contribute to electric energy savings. However, the present dehumidification system has many weak points. The liquid desiccant air-conditioning system has recently gained growing interest from the stand point of reducing energy consumption during dehumidification. In order to find the appropriate ionic liquids(ILs) as a desiccant for the liquid desiccant air-conditioner system, we conducted a systematic evaluation of the humidification capability of 16 types of ILs. Among the tested ILs, tributyl(methyl)phosphonium dimethyl phosphate([P4441][DMPO_4]) exhibited the best dehumidification capacity and had a less corrosive effect on four types of metals as possible piping materials. It should be noted that this [P_(4441)][DMPO_4] has a very stable nature and produced no odor while conducting the experiment and storing for over 1 year at room temperature under ambient conditions. Furthermore, it was revealed that a 77%(w/w) aqueous solution of [P4441][DMPO_4] worked as an efficient desiccant liquid for the liquid desiccant air-conditioner system.

关键词： Dehumidification Liquid desiccant Ionic liquids Air-conditioner system Phosphonium ILs

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Dose-response effects of in-feed antibiotics on growth performance and nutrient utilization in weaned pigs fed diets supplemented with yeast-based nucleotides

引用

Animal Nutrition 2015年第3期1卷 166-169页

作者： Samuel M.Waititu Jung M.Heo Rob Patterson Charles M.Nyachoti Department of Animal Science University of Manitoba Department of Animal Science and biotechnology Chungnam National University Canadian Bio-systems inc.

Dietary nucleotides are bioactive compounds with the potential to mitigate weaning-associated challenges in piglets. An experiment was conducted to determine the interaction effect of antimicrobial growth promoters(AGP) and a nucleotide-rich yeast extract(NRYE) on growth performance and apparent total tract digestibility(ATTD) of dry matter(DM), crude protein(CP) and gross energy(GE),and to establish whether NRYE supplementation may completely or partially replace AGP in diets for weaned pigs. In phase 1 and 2, corn, wheat, canola meal and soybean meal based diets, which were formulated to contain 0.0 or 0.1% NRYE with 0, 25, 50, 75 or 100% of the recommended AGP dosage, were fed to 108 twenty-one day old piglets(initial body weight 7.11 ± 0.9 kg; mean ±SD) from d 1 to 14 and15 to 28, respectively. Overall, inc.easing AGP level in NRYE supplemented diets linearly decreased average daily gain(ADG)(P = 0.002) and gain-to-feed ratio(G:F)(P = 0.007); and quadratically decreased ATTD of DM(P = 0.001), CP(P =0.003) and G:F(P = 0.017) during phase 2. Compared with control and pigs fed NRYE with 100% of recommended AGP dosage, pigs fed 0.1% NRYE without AGP had greater(P inc.easing dietary AGP dosage.

关键词： Antimicrobial growth promoters Digestibility Growth performance Piglet Nucleotide-rich yeast extract

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：