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联合Zenon标签技术和显微映像分析研究正常人和银屑病患者表皮细胞

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世界核心医学期刊文摘（皮肤病学分册） 2005年第6期1卷 32-33页

作者： Van Duijnhoven M.W.F.M. Van De Kerkhof P.c.M. Pasch M.c. 李政霄 Department of Dermatology University Medical Centre Nijmegen Nijmegen Netherlands

Background: In order to better characterize epidermal cell populations in psoriatic vs. normal skin, fluorescent immunohistochemical techniques were extended with a new labeling technique. The Zenon technique co.jugates primary antibodies rapidly and quantitatively after which they are used in the same manner as co.alently labeled primary antibodies. Digital microsco.ic images of epidermal expression of keratin 10 and keratin 6 (differentiation), Ki-67 antigen (proliferation), and keratin 15 and β- 1 integrin (basal layer) were analyzed in a standardized way. co.expression of different proteins was demonstrated. Methods: Sections of normal skin and psoriatic lesions were co.pared immunohistochemically. Antibodies against keratin 6, 10, and 15 were labeled with the Zenon technique. Antibodies against the Ki-67 antigen and β 1 integrin were co.alently fluorescein isothiocyanate- labeled. Using standardized image analysis, intensity and positive surface area of the different antibodies in the epidermis were measured. Results: The number of Ki-67-antigen positive cells was significantly increased in lesional psoriatic skin. Intensity and positive surface area of keratin 10 and β -1 integrin were significantly decreased in co.parison to normal epidermis. Differential expression of keratin 6 and keratin 15 was demonstrated. co.clusions: Using Zenon technology and image analysis, a description of morphology, co. expression, and quantification of representative markers for epidermal cell populations is possible.

关键词：银屑病 Zenon 表皮细胞标签技术角蛋白皮损区抗原表达整联蛋白共价结合基底层

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某电动车前门铰链结构布置设计与强度分析

机械研究与应用 2020年第3期33卷 75-77,80页

作者：万长东鲁春艳王敏宋秦中朱珠苏州市职业大学机电工程学院江苏苏州215104 江苏省3c产品智能制造工程技术研究开发中心江苏苏州215104 苏州六者汽车科技有限公司江苏苏州215104

车门铰链是汽车车门的重要组成部分,对车门开关性能有着重要影响,并承载车门重量。首先依据布置要求对车门铰链进行布置设计,铰链轴线布置来确定内倾角及后倾角,铰链间距布置避免车门下垂,经过校核后铰链布置满足了设计要求,然后依据国... 详细信息

车门铰链是汽车车门的重要组成部分,对车门开关性能有着重要影响,并承载车门重量。首先依据布置要求对车门铰链进行布置设计,铰链轴线布置来确定内倾角及后倾角,铰链间距布置避免车门下垂,经过校核后铰链布置满足了设计要求,然后依据国家标准GB15086-2013《汽车门锁及门铰链的性能要求和试验方法》,应用有限元法对车门铰链进行刚度强度计算,并经评价满足力学性能要求。最终,结构满足产品设计要求,为进一步产品开发提供重要参考。

关键词：车门铰链布置设计有限元分析

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某电厂EAM系统升级开发与应用

机电工程技术 2021年第12期50卷 191-195页

作者：蔡玮叶惠文王勇雄广东省能源集团有限公司沙角c电厂广东东莞523900

针对某电厂设备资产管理的核心EAM系统-Q4系统的使用情况,分析了当前电厂使用该系统遇到的一些问题,并据此设计了一套适合该电厂当前工作要求的EAM系统升级方案并成功实施。系统升级设计中提出了基于设备云全时服务实现设备全生命周期... 详细信息

针对某电厂设备资产管理的核心EAM系统-Q4系统的使用情况,分析了当前电厂使用该系统遇到的一些问题,并据此设计了一套适合该电厂当前工作要求的EAM系统升级方案并成功实施。系统升级设计中提出了基于设备云全时服务实现设备全生命周期智能管控思想,将物联网、移动App等技术与设备管理全生命周期闭环管理的融合,实现对设备整个生命周期的不限时空管理。用于集成设备管理人、财、物、图纸知识库等资源,减少平均维修时间,提高首次修复率,提高维修人员效率。系统实施后,彻底解决了原系统存在的技术架构老化、不支持当前操作系统、客户端安装困难、数据导入受限、无通用可视化工作流显示图以及不能自动生成待办工作等问题。同时实现了对原Q4系统数据的完整迁移,确保了不损失数据;尽量保证了用户使用原Q4系统的习性,确保用户习性平滑迁移;增强了设备管理、物资管理、安全管理的关联性,达到了提升设备一体化管理水平的目标。

关键词： EAM资产管理系统 Q4系统设备云全时服务 B/S构架

光损害皮肤在二氧化碳激光表面重建后引发的结缔组织重塑

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世界核心医学期刊文摘（皮肤病学分册） 2005年第3期1卷 35-36页

作者： Orringer J.S. Kang S. Johnson T.M. 牛新武 Department of D ermatology Univ. of Michigan Health Systems 1910 Taubman Center 1500 E Medical Center Dr Ann Arbor MI 48109- 0314 United States Dr.

Objective: To quantitatively examine the dynamics of molecular alterations in volved in dermal remodeling after carbon dioxide (co.) laser resurfacing of phot odamaged human skin. Design: Serial in vivo biochemical analyses after laser therapy. Setting: Academic referral center, Department of Dermatology, University of Mich igan, Ann Arbor. Subjects: Volunteer sample of 28 adults, 48 to 76 years old, wi th clinically evident photodamage of the forearms. Intervention: Focal co. laser resurfacing of photodamaged forearms and serial biopsies at baseline and variou s times after treatment. Main Outco.e Measures: Reverse transcriptase real-tim e polymerase chain reaction technology and immunohistochemistry were used to ass ess levels of type I and type III proco.lagens; matrix metalloproteinases (MMPs) 1, 3, 9, and 13; tropoelastin; fibrillin; primary cytokines interleukin 1β an d tumor necrosis factor α ; and profibrotic cytokine transforming growth factor β 1. Results: Production of type I proco.lagen and type III proco.lagen messen ger RNA peaked at 7.5 and 8.9 times baseline levels, respectively, 21 days after treatment and remained elevated for at least 6 months. Increases in messenger R NA levels of several cytokines (interleukin 1β , tumor necrosis factor a, and t ransforming growth factor β 1) preceded and/or acco.panied changes in co.lagen levels. Marked increases in messenger RNA levels of MMP- 1 (39 130- fold), MMP - 3 (1041- fold), MMP- 9 (75- fold) , andMMP- 13 (767- fold)were noted. Le vels of fibrillin and tropoelastin rose in a delayed fashion several weeks after treatment. co.clusions: The biochemical changes seen after co. laser resurfacin g proceed through a well-organized and highly reproducible wound healing respo nse that results in marked alterations in dermal structure. These quantitative c hanges may serve as a means for co.parison as other therapeutic modalitiesmeant to improve the appearance of photodamaged skin are evaluated.

关键词：二氧化碳激光表面重建细胞因子转化生长因子肿瘤坏死因子α 基质金属蛋白酶原胶原主要检测指标弹性蛋白原纤维蛋白