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OsADR3 increases drought stress tolerance by inducing antioxidant defense mechanisms and regulating OsGPX1 in rice(Oryza sativa L.)

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The Crop Journal 2021年第5期9卷 1003-1017页

作者： Jiaming Li Minghui Zhang Luomiao Yang Xinrui Mao Jinjie Li Lu Li Jingguo Wang Hualong Liu Hongliang Zheng Zichao Li Hongwei Zhao Xianwei Li Lei Lei Jian Sun Detang Zou Key laboratory of Germplasm Enhancement Physiology and Ecology of Food Crops in Cold RegionMinistry of EducationNortheast Agricultural UniversityHarbin 150030HeilongjiangChina College of Life Science Northeast Agricultural UniversityHarbin 150030HeilongjiangChina Key laboratory of Crop Heterosis and Utilization of Ministry of Education and Beijing Key lab of Crop Genetic Improvement China Agricultural UniversityBeijing 100094China

The C (Cys) 2H (His) 2-type transcription factor is one of the most important transcription factors in plants and plays a regulatory role in the physiological responses of rice to abiotic stresses.A novel rice C2H2-type zinc finger protein,abscisic acid (ABA)-drought-reactive oxygen species (ROS) 3 (OsADR3),was found to confer drought stress tolerance by enhancing antioxidant defense and regulating Os *** of OsADR3 in rice increased tolerance to drought stress by increasing ROS scavenging ability and ABA *** contrast,CRISPR/Cas9-mediated knockout of osadr3 increased the sensitivity of rice to drought and oxidative *** exogenous ROS-scavenging reagent restored the droughtstress tolerance of osadr3-CRISPR *** transcriptome analysis suggested that OsADR3 increased the expression of Os GPX1 under drought *** mobility shift,yeast one-hybrid,and dualluciferase reporter assays revealed that OsADR3 modified the expression of Os GPX1 by directly binding to its *** of Os GPX1 repressed ROS scavenging ability under drought stress in OsADR3-overexpression *** findings suggest that OsADR3 plays a positive regulatory role in droughtstress tolerance by inducing antioxidant defense and associated with the ABA signaling pathway in rice.

关键词： Drought stress Rice ROS scavenging Glutathione peroxidase Abscisic acid

Analysis of Short-Term Metabolic Alterations in Arabidopsis Following Changes in the Prevailing Environmental Conditions

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Molecular Plant 2014年第5期7卷 893-911页

作者： Alexandra Florian Zoran Nikoloski Ronan Sulpice Stefan Timm Wagner L. Araujo Takayuki Tohge Hermann Bauwe Alisdair R. Fernie Max-Planck-Institut for Molekulare Pflanzenphysiologie Am Muhlenberg 1 14476 Potsdam-Golm Germany NUIG Plant Systems Biology Lab Plant and AgriBiosciences Research Centre Botany and Plant Science Galway Ireland Department of Plant physiology University of Rostock D-18059 Rostock Germany Departamento de Biologia Vegetal Universidade Federal de Vicosa 36570-000 Vicosa Minas Gerais Brazil

Although a considerable increase in our knowledge concerning the importance of metabolic adjustments to unfavorable growth conditions has been recently provided, relatively little is known about the adjustments which occur in response to fluctuation in environmental factors. Evaluating the metabolic adjustments occurring under changing environmental conditions thus offers a good opportunity to increase our current understanding of the crosstalk between the major pathways which are affected by such conditions. To this end, plants growing under normal conditions were transferred to different light and temperature conditions which were anticipated to affect （amongst other processes） the rates of photosynthesis and photorespiration and characterized at the physiological, molecular, and metabolic levels following this transition. Our results revealed similar behavior in response to both treatments and imply a tight connec- tivity of photorespiration with the major pathways of plant metabolism. They further highlight that the majority of the regulation of these pathways is not mediated at the level of transcription but that leaf metabolism is rather pre-poised to adapt to changes in these input parameters.

关键词： central metabolism environmental perturbation gene expression metabolic regulation photorespiration photosynthesis ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO).

Progress in atherosclerotic plaque imaging

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World Journal of Radiology 2012年第8期4卷 353-371页

作者： Giulia Soloperto Sergio Casciaro Biomedical Engineering Science and Technology Division and Nanoimaging Ultrasound lab at National Research Council Institute of Clinical Physiology(CNR-IFC)73100 LecceItaly

Cardiovascular diseases are the primary cause of mortality in the industrialized world,and arterial obstruction, triggered by rupture-prone atherosclerotic plaques,lead to myocardial infarction and cerebral *** plaques do not necessarily occur with flow-limiting stenosis,thus conventional luminographic assessment of the pathology fails to identify unstable *** this review we discuss the currently available imaging modalities used to investigate morphological features and biological characteristics of the atherosclerotic plaque. The different imaging modalities such as ultrasound, magnetic resonance imaging,computed tomography, nuclear imaging and their intravascular applications are illustrated,highlighting their specific diagnostic *** available and upcoming methodologies are also reviewed along with the related challenges in their clinical translation,concerning the specific invasiveness, accuracy and cost-effectiveness of these methods.

关键词： Atherosclerosis Diagnostic imaging Plaque characterization

Functional analysis of helicase and three tandem HRDC domains of RecQ in Deinococcus radiodurans

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2006年第5期7卷 373-376页

作者： HUANG Li-fen HUA Xiao-ting LU Hui-ming GAO Guan-jun TIAN Bing SHEN Bing-hui HUA Yue-jin Institute of Nuclear-Agricultural Science Zhejiang University Hangzhou 310029 China Jiangsu Province Key lab of Crop Genetics and physiology Yangzhou University Yangzhou 225009 China Division of Molecular Biology City of Hope National Medical Center and Beckman Research Institute Duarte CA 91010-3000 USA

RecQ is a highly conserved helicase necessary for maintaining genome stability in all organisms. Genome comparison showed that a homologue of RecQ in Deinococcus radiodurans designated as DR1289 is a member of RecQ family with unusual domain arrangement: a helicase domain, an RecQ C-terminal domain, and surprisingly three HRDC domain repeats, whose func-tion, however, remains obscure currently. Using an insertion deletion, we discovered that the DRRecQ mutation causes an increase in gamma radiation, hydroxyurea and mitomycine C and UV sensitivity. Using the shuttle plasmid pRADK, we complemented various domains of the D. radiodurans RecQ (DRRecQ) to the mutant in vivo. Results suggested that both the helicase and helicase-and-RNase-D-C-terminal (HRDC) domains are essential for complementing several phenotypes. The complementation and biochemical function of DRRecQ variants with different domains truncated in vitro suggested that both the helicase and three HRDC domains are necessary for RecQ functions in D. radiodurans, while three HRDC domains have a synergistic effect on the whole function. Our finding leads to the hypothesis that the RecF recombination pathway is likely a primary path of double strand break repair in this well-known radioresistant organism.

关键词： RecQ Deinococcus radiodurans Helicase HRDC

施氮量和花后土壤含水量对优质强筋小麦产量和品质的影响

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生态学报 2008年第9期28卷 4396-4404页

作者：赵长星马东辉王月福林琪吴钢邵宏波 Cheruth Abdul JALEEL 青岛农业大学植物科技学院中国科学院生态环境研究中心城市与区域生态国家重点实验室青岛科技大学生命科学研究所 stress physiology lab Department of Botany Annamalai UniversityAnnamalainagar608002TamilnaduIndia

在防雨池栽条件下,研究了施氮量和花后土壤含水量对优质强筋小麦产量和品质的影响。结果表明,在同一施氮量条件下,表现为花后土壤含水量过高（80%～90%）或过低（40%～50%）导致穗粒数减少,千粒重降低,最终使产量降低。在同一土壤含水量... 详细信息

在防雨池栽条件下,研究了施氮量和花后土壤含水量对优质强筋小麦产量和品质的影响。结果表明,在同一施氮量条件下,表现为花后土壤含水量过高（80%～90%）或过低（40%～50%）导致穗粒数减少,千粒重降低,最终使产量降低。在同一土壤含水量下,表现为增加施氮量有利于提高穗数,但过多（300kg/hm^2）或过少（150kg/hm^2）施氮均不利于穗粒数和千粒重的提高,而导致减产。在同一土壤含水量下,总蛋白质、醇溶蛋白、麦谷蛋白含量及谷/醇比随着施氮量的增加而增加。在同一施氮量条件下,总蛋白质及各组分均随着土壤含水量的增加而降低,同时谷/醇比也降低。在同一施氮量下,花后土壤含水量过高（80%～90%）或过低（40%～50%）均不利于淀粉及其组分含量的提高。在同一土壤含水量下,过高（300kg/hm^2）或过低（150kg/hm^2）施用氮肥均不利于淀粉及其组分含量的提高。只有保持适宜的花后土壤含水量和施适宜的氮肥才有利于支/直比的提高。适量增施氮肥或花后土壤含水量适宜可提高小麦的加工品质。这说明在小麦生产中可以通过施用氮肥和控制花后土壤水分含量技术,调控小麦品质和产量的形成,从而实现优质高产。

关键词：施氮量土壤含水量小麦产量品质

Caffeine Increases Rate of Torque Development Without Affecting Maximal Torque

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Journal of Science in Sport and Exercise 2019年第3期1卷 248-256页

作者： B rent M.Peterson Lee E.Brown Daniel A.Judelson Samantha Gallo-Rebert Jared W.Coburn Department of Kinesiology School of ScienceTechnologyand HealthBiola UniversityLa MiradaCAUSA Exercise physiology lab and Center for Sport Performance Department of KinesiologyCalifornia State UniversityFullerton800 N.State CollegeBlvd.KHS-235CA 92834USA

Purpose The purpose of this study was to examine the effects of caffeine on peak torque(PT),rate of torque development(RTD),and muscle activation in college-age *** Fifteen men(23.3±2.2 years,177.6±8.7 cm,90.3±19.1 kg)volunteered to participate in this *** each of two test sessions,maximal leg extension PT and RTD were determined and electromyographic(EMG)and mechano-myographic(MMG)signals were collected to examine electrical and mechanical aspects of muscle activation,*** also performed the Wingate Anaerobic Test(WAnT)for the determination of peak power(PP),mean power(MP),and fatigue percentage(FP).For the first test session,participants were randomly assigned to ingest either a caffein-ated drink(6 mg/kg)or a placebo 1 h prior to *** second test session was identical to the first,but the drink not previously administered was ingested prior to *** The results indicated there were no significant effects of caffeine on PT,PP,MP,FP,or EMG and MMG ***,RTD was positively affected by caffeine(856.4±246.4 Nm/s)compared to the placebo condition(710.9±267.9 Nm/s).Conclusions These results suggest that caffeine can be an effective aid for sport activities requiring maximal rates of force or torque development during very short time periods.

关键词： Ergogenic aid Wingate anaerobic test Electromyography Mechanomyography

Cloning and characterization of vernalization-related gene (ver203F) cDNA 3'end

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Chinese Science Bulletin 1999年第14期44卷 1289-1294页

作者： YONG Weidong, CHONG Kang , LIANG Tiebing, XU Zhihong , TAN Kehui and ZHU Zhiqing Research Center for Molecular and Developmental Biology, Institute of Botany, Chinese Academy of Sciences , Beijing 100093, China Shanghai Institute of Plant physiology , Chinese Academy of Sciences , Shanghai 200032, China Corresponding authors Research Center for Molecular and Developmental Biology Institute of Botany Chinese Academy of Sciences Beijing China Shanghai Institute of Plant physiology Chinese Academy of Sciences Shanghai China

Based on the cDNA fragment sequence of vernalization-related gene verc203 cloned by differential screening in our lab, the 5′primer has been designed. The cDNA 3′end of ver203 gene (1 197 bp) has been cloned by the RACE method. And it is identified by Northern blotting that its expression is special in vernalization treatment. After comparing the sequence in the nucleotide sequence databases of Genbank, EMBL and DDBJ, the gene has homology with Hordeum vulgare jesmonate-induced protein gene. It is suggested that this gene might be related to the signal transduction mediated by jamonate.

关键词： wheat vernalization-related gene cDNA 3’ end RACE.

Fatty acid oxidation and autophagy promote endoxifen resistance and counter the effect of AKT inhibition in ER-positive breast cancer cells

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Journal of Molecular Cell Biology 2021年第6期13卷 433-444页

作者： Lei Duan Sarah Calhoun Daeun Shim Ricardo E.Perez Lothar A.Blatter Carl G.Maki Department of Cell and Molecular Medicine Rush University Medical CenterChicagoIL 60612USA Department of Molecular Biophysics and physiology Rush University Medical CenterChicagoIL 60612USA

Tamoxifen(TAM)is the first-line endocrine therapy for estrogen receptor-positive(ER+)breast cancer(BC).However,acquired resistance occurs in∼50%***,although the PI3K/AKT/mTOR pathway is a viable target for treatment of endocrine therapy-refractory patients,complex signaling feedback loops exist,which can counter the effectiveness of inhibitors of this ***,we analyzed signaling pathways and metabolism in ER+MCF7 BC cell line and their TAM-resistant derivatives that are co-resistant to endoxifen using immunoblotting,quantitative polymerase chain reaction,and the Agilent Seahorse XF *** found that activation of AKT and the energy-sensing kinase AMPK was increased in TAM and endoxifen-resistant ***,ERRα/PGC-1βand their target genes MCAD and CPT-1 were increased and regulated by AMPK,which coincided with increased fatty acid oxidation(FAO)and autophagy in TAM-resistant *** of AKT feedback-activates AMPK and ERRα/PGC-1β-MCAD/CPT-1 with a consequent increase in FAO and autophagy that counters the therapeutic effect of endoxifen and AKT ***,our results indicate increased activation of AKT and AMPK with metabolic reprogramming and increased autophagy in TAM-resistant *** inhibition of AKT and FAO/autophagy is necessary to fully sensitize resistant cells to endoxifen.

关键词： endoxifen fatty acid oxidation autophagy AMPK AKT

The role of nitric oxide in the dorsomedial periaqueductal gray(dmPAG)column in cardiovascular responses in urethane-anesthetized male rats

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Animal Models and Experimental Medicine 2022年第6期5卷 557-564页

作者： Mohammad Najaftomaraei Atiyeh Ghorbani Alireza Rahimi Reza Mohebbati Sogol Sherkat Mohammad Naser Shafei Department of physiology Faculty of MedicineMashhad University of Medical SciencesMashhadIran Material Science and Metallurgy Engineering Islamic Azad University-Karaj BranchKarajIran Department of physiology Faculty of MedicineGonabad University of Medical SciencesGonabadIran Applied Biomedical Research Center Mashhad University of Medical SciencesMashhadIran Department of physiology School of MedicineSabzevar University of Medical SciencesSabzevarIran Division of Neurocognitive Sciences Psychiatry and Behavioral Sciences Research CenterMashhad University of Medical SciencesMashhadIran

Background:The dorsomedial periaqueductal gray(dmPAG)is a mesencephalic area and has numerous functions including cardiovascular *** nitric oxide(NO)is present in the dmPAG,here we investigate,the probable cardiovascular effect of NO in the ***:Five groups(n=6 for each group)were used as follows:(1)control;(2)L-NAME(N^(G)-nitro-L-arginine methyl ester,a NO synthase inhibitor,90 nmol);(3)Larginine(L-Arg,a precursor for NO,60 nmol);(4)Sodium nitroprusside(SNP,a NO donor,27 nmol);and(5)L-Arg+*** cardiovascular parameters were recorded by a Power lab device after cannulation of the femoral *** were injected using a stereotaxic *** changes(Δ)in systolic blood pressure(SBP),mean arterial pressure(MAP),and heart rate(HR)were calculated at different times and compared to the control ***:Microinjection of L-NAME significantly increased ΔSBP,ΔMAP,and ΔHR more than saline(from p<0.05 to p<0.001).L-Arg only significantly increased ΔHR(p<0.05).In the L-Arg+L-NAME group,the above parameters also significantly increased(from p<0.01 to p<0.05)but not as significantly as with L-NAME *** of SNP significantly decreased ΔSBP and ΔMAP more than in the control and L-NAME groups(from p<0.01 to p<0.001),but ΔHR did not change ***:The results indicated that NO in dmPAG has an inhibitory effect on cardiovascular responses in anesthetized rats.

关键词： blood pressure dorsomedial periaqueductal gray L-arginine L-NAME nitric oxide