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Effects of Transgenic Tobacco Plants Expressing ACA Gene from Amaranthus caudatus on the Population Development of Myzus persicae

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Acta Botanica Sinica 2004年第9期46卷 1100-1105页

作者： GUOHong-Nian JIAYan-Tao ZHOUYong-Gang ZHANGZhen-Shan OUYANGQing JIANGYing TIANYing-Chuan statekeylaboratoryofplantgenomics InstituteofMicrobiologyTheChineseAcademyofSciencesBeijing100080China

To investigate the possible function of the agglutinin from Amaranthus caudatus L. (ACA) in plant defending against insect pests, ACA cDNA was cloned by RT-PCR and the 5' and 3' sequences were confirmed by rapid amplification of cDNA ends (RACE). The phloem-specific expression vector of ACA gene, pBCACAc, was constructed based on the plant binary vector pBC438 and transfered into tobacco plants via Agrobacterium-mediated transformation method. Results from PCR and Southern blotting analysis showed that AOA gene was integrated into the genomes of transformed plants and the transgene integration varied from one to four estimated copies per genome. Western blotting analysis indicated that ACA gene was transcribed and translated in the transgenic plants. The bioassay of Myzus persicae Sulzer on detached leaves demonstrated that the 78% transgenic tobacco plants displayed an average aphid-resistant rate of more than 75%. Some apterous progeny of M. persicae were found dead on the resistant plants. These results indicate that ACA gene should be an effective aphid-resistant gene and could be valuable for application in crop breeding for aphid resistance.

关键词：转基因烟草植株表达苋属 ACA基因蚜虫抗性

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Contribution of Microbial Inter-kingdom Balance to Plant Health

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Molecular Plant 2019年第2期12卷 148-149页

作者： Chunlei Zhang Youming Zhang Zhaojun Ding Yang Bai StateKeyLaboratoryofMicrobialTechnology ShandongUniversityQingdaoShandong266237China statekeylaboratoryofplantgenomics InstituteofGeneticsandDevelopmentalBiologyChineseAcademyofScienceBeijing100101China CAS-JICCentreofExcellenceforPlantandMicrobialScience InstituteofGeneticsandDevelopmentalBiologyChineseAcademyofSciencesBeijing100101China “UniversityofChineseAcademyofSciences CollegeofAdvaneedAgriculturalSciencesBeijing100039China TheKeyLaboratoryofPlantDevelopmentandEnvironmentalAdaptationBiology MinistryofEducationCollegeofLifeSciencesShandongUniversityQingdaoShandong266237China

Soil-borne fungal and oomycetal pathogens cause devastating agricultural losses every year. The abundance and diversity of filamentous eukaryotes in the roots of healthy plants are in flue need by root-associated bact... 详细信息

关键词： Microbial Inter-kingdom Balance Plant Health filamentous eukaryotes

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