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Mechanical Properties of Breast Cancer Cell Membrane Studied with Optical Tweezers

Chinese Physics Letters 2004年第12期21卷 2543-2546页

作者：郭红莲刘春香段建发隆雨强韩学海李兆霖程丙英张道中 OpticalPhysicsLaboratory InstituteofPhysicsChineseAcademyofSciencesBeijing100080 statekeylaboratoryofbiomacromolecles InstituteofBiophysicsChineseAcademyofSciencesBeijing100101

Membrane tethers are extracted from breast cancer cells using a force generated by an optical trap. It is experimentally obtained that the radius of tether is about 0.1μm and the static tether force is about 8.5 pN. Calculations based on the experimental measurements give a bending modulus for the tether of 1.35×10-19Nm and a surface membrane tension of 6.76×10-6N/m in the breast cancer cell. The treatment with cytochalasin D results in the decreasing bending modulus and decreasing apparent surface tension. When the membrane protein caveolin is over-expressed, similar cases occur in bending modulus and apparent surface tension. In addition, the viscous resistance coefficient of the membrane is calculated to be 1.15pN. s/μm according to the dynamic tether forces obtained under different pulling velocities.

关键词： Surface tension

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Displacement and Force Measurements with Quadrant Photodetector in Optical Tweezers

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Chinese Physics Letters 2003年第6期20卷 950-952页

作者： GUOHong-Lian LIUChun-Xiang LIZhao-Lin DUANJian-Fa HANXue-Hai CHENGBing-Ying ZHANGDao-Zhong OpticalPhysicsLaboratory InstituteofPhysicsandCenterforCondensedMatterPhysicsChineseAcademyofSciencesBeijing100080 statekeylaboratoryofbiomacromolecles InstituteofBiophysicsChineseAcademyofSciencesBeijing100101

A technique of displacement and force measurements with a photodiode quadrant detector in an optical tweezers system is presented. The stiffness of optical trap is calibrated and the leukemia cell membrane tension is measured. The results show that the optical tweezers combined with the quadrant detector is a very useful tool for detecting the displacement and force with a millisecond-order response.

关键词： Optical tweezers

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Measurements of leucocyte membrane elasticity based on the optical tweezers

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Chinese Science Bulletin 2003年第5期48卷 503-508页

作者： GUOHonglian CAOQinhong RENDongtao LIUGuoqing DUANJianfa LIZhaolin ZHANGDaozhong HANXuehai OpticalPhysicsLaboratory InstituteofPhysicsandCenterforCondensedMatterPhysicsChineseAcademyofSciencesBeijing100080China StateKeyLoboratoryofPlantPhysiologyandBiochemistry CollegeofBiologySciencesChinaAgriculturalUniversityBeijing100094China statekeylaboratoryofbiomacromolecles InstitueofBiophysicsChineseAcademyofSciencesBeijing100101China statekeylaboratoryofbiomacromolecles InstituteofBiophysicsChineseAcademyofSciencesBeijing100101China

A 1-mm in diameter polystyrene bead coated with lectin is trapped by optical tweezers which is formed by a focused laser beam (740 nm). A leucocyte adhered to the bottom of sample cell is chosen to close to the trapped bead. When their stable combination is confirmed, the leucocyte is displaced by moving the sample stage. A tether is then formed between the trapped bead and the membrane. The force acting on the tether is measured by a detector equipped on the optical tweezers system. The deformation of the membrane is diverse in different contact conditions. When the contact area is small, the tether is very thin and the force on it is 7.4 pN (10-12 N), while the tether is much thicker un-der a larger contact area and its corresponding force is about 14 pN. It is presumed that the latter is related to the defor-mation of cytoskeleton.

关键词：白细胞细胞膜弹力测量光学镊子系统细胞骨架血液细胞

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