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GOLDEN 2-LIKE transcription factors regulate chlorophyll biosynthesis and flavonoid accumulation in response to UV-B in tea plants

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Horticultural Plant Journal 2023年第5期9卷 1055-1066页

作者： Xuyang Liu Xin Cheng Jingjie Cao Wenfeng Zhu Xiaochun Wan Linlin Liu state key laboratory of tea Plant biology and utilization Key Laboratory of Tea Biology and Tea Processing of Ministry of AgricultureAnhui Provincial Laboratory of Tea Plant Biology and UtilizationAnhui Agricultural UniversityHefeiAnhui 230036China

Flavonoids are critical secondary metabolites that determine the health benefits and flavor of tea,while chlorophylls are important contributors to the appearance of ***,transcription factors(TFs)that can integrate both chlorophyll biosynthesis and flavonoid accumulation in response to specific light signals are rarely *** this study,we report that the GOLDEN 2-LIKE TF pair,CsGLK1 and CsGLK2,orchestrate UV-B-induced responses in the chlorophyll biosynthesis and flavonoid accumulation of tea *** absence of solar UV-B reduced the transcriptional expression of CsGLKs in the tea leaves and was highly correlated with a decrease in flavonoid levels(especially flavonol glycosides)and the expression of genes and TFs involved in chlorophyll biosynthesis and flavonoid *** vivo and in vitro molecular analyses showed that CsGLKs could be regulated by the UV-B signal mediator CsHY5,and could directly bind to the promoters of gene and TF involved in light-harvesting(CsLhcb),chlorophyll biosynthesis(CsCHLH,CsHEMA1,and CsPORA),and flavonoid accumulation(CsMYB12,CsFLSa,CsDFRa,and CsLARa),eventually leading to UV-B-induced responses in the chlorophylls and flavonoids of tea ***,UV-B exposure increased the levels of total flavonoids,CsGLK1 protein,and expression of CsGLKs and target genes in the tea *** results indicate that CsGLKs may modulate tea leaf characteristics by regulating chlorophyll biosynthesis and flavonoid accumulation in response to solar *** the first report on UV-B-induced changes in flavonoid and chlorophyll regulation mediated by CsGLKs,this study improves our understanding of the environmental regulations regarding tea quality and sheds new light on UV-B-induced flavonoid responses in higher plants.

关键词： Camellia sinensis GLK Flavonoid Chlorophyll UV-B

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JA-mediated MYC2/LOX/AOS feedback loop regulates osmotic stress response in tea plant

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Horticultural Plant Journal 2024年第3期10卷 931-946页

作者： Junyan Zhu Hongrong Chen Lu Liu Xiaobo Xia Xiaomei Yan Xiaozeng Mi Shengrui Liu Chaoling Wei state key laboratory of tea Plant biology and utilization/key laboratory of tea biology and tea Processing of Ministry of Agriculture/Anhui Provincial laboratory of tea Plant biology and utilization Anhui Agricultural UniversityHefeiAnhui 230036China

Osmotic stress caused by low-temperature,drought and salinity was a prevalent abiotic stress in plant that severely inhibited plant development and agricultural yield,particularly in tea *** acid(JA)is an important phytohormone involving in plant ***,underlying molecular mechanisms of JA modulated osmotic stress response remains *** this study,high concentration of mannitol induced JA accumulation and increase of peroxidase activity in tea *** transcriptome mined a JA signaling master,MYC2 transcription factor is shown as a hub regulator that induced by mannitol,expression of which positively correlated with JA biosynthetic genes(LOX and AOS)and peroxidase genes(PER).CsMYC2 was determined as a nuclei-localized transcription activator,furthermore,ProteinDNA interaction analysis indicated that CsMYC2 was positive regulator that activated the transcription of CsLOX7,CsAOS2,CsPER1 and CsPER3via bound with their promoters,*** of CsMYC2 expression resulted in a reduced JA content and peroxidase activity and osmotic stress tolerance of tea *** of CsMYC2 in Arabidopsis improved JA content,peroxidase activity and plants tolerance against mannitol ***,we proposed a positive feedback loop mediated by CsMYC2,CsLOX7 and CsAOS2 which constituted to increase the tolerance of osmotic stress through fine-tuning the accumulation of JA levels and increase of POD activity in tea plant.

关键词： Camellia sinensis Jasmonic acid(JA) MYC2 transcription factor Lipoxygenase(LOX) Osmotic stress Peroxidase(POD) Reactive oxygen species(ROS)

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Core collection construction of tea plant germplasm in Anhui Province based on genetic diversity analysis using simple sequence repeat markers

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Journal of Integrative Agriculture 2023年第9期22卷 2719-2728页

作者： TAO Ling-ling TING Yu-jie CHEN Hong-rong WEN Hui-lin XIE Hui LUO Ling-yao HUANG Ke-lin ZHU Jun-yan LIU Sheng-rui WEI Chao-ling state key laboratory of tea Plant biology and utilization/key laboratory of tea biology and tea Processing of Ministry of Agriculture and Rural Affairs/Anhui Provincial laboratory of tea Plant biology and utilization Anhui Agricultural UniversityHefei 230036P.R.China

The tea plant[Camellia sinensis(L.)***]is an industrial crop in *** Anhui Province has a long history of tea cultivation and has a large resource of tea germplasm with abundant genetic *** reduce the cost of conservation and utilization of germplasm resources,a core collection needs to be *** this end,573 representative tea accessions were collected from six major tea-producing areas in Anhui *** on 60 pairs of simple sequence repeat(SSR)markers,phylogenetic relationships,population structure and principal coordinate analysis(PCoA)were *** analysis indicated that the 573 tea individuals clustered into five groups were related to geographical location and were consistent with the results of the ***,we constructed a core collection consisting of 115 tea individuals,accounting for 20%of the whole *** 115 core collections were considered to have a 90.9%retention rate for the observed number of alleles(Na),and Shannon’s information index(I)of the core and whole collections were highly *** these,39 individuals were preserved in the Huangshan area,accounting for 33.9%of the core collection,while only 10 individuals were reserved in the Jinzhai County,accounting for 8.9%of the core *** of the accessions in the tea plant core collection exhibited a pattern nearly identical to that of the accessions in the entire collection,further supporting the broad representation of the core germplasm in Anhui *** results demonstrated that the core collection could represent the genetic diversity of the original *** present work is valuable for the high-efficiency conservation and utilization of tea plant germplasms in Anhui Province.

关键词： tea plant core collection genetic diversity SSR markers

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CsGDH2.1 negatively regulates theanine accumulation in late-spring tea plants (Camellia sinensis var. sinensis)

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Horticulture Research 2023年第1期10卷 240-248页

作者： Tingting Chen Jingzhen Ma Huiping Li Shijia Lin Chunxia Dong Yunxia Xie Xiaomei Yan Shupei Zhang Tianyuan Yang Xiaochun Wan Zhaoliang Zhang state key laboratory of tea Plant biology and utilization Anhui Agricultural UniversityHefei 230036China

Theanine,a unique and the most abundant non-proteinogenic amino acid in tea plants,endows tea infusion with the umami taste and anti-stress *** content in tea correlates highly with green tea *** content in new shoots of tea plants is high in mid-spring and greatly decreases in late ***,how the decrease is regulated is largely *** a genetic screening,we observed that a yeast mutant,glutamate dehydrolase 2(gdh2),was hypersensitive to 40 mM theanine and accumulated more *** result implied a role of CsGDH2s in theanine accumulation in tea ***,we identified the two homologs of GDH2,CsGDH2.1 and CsGDH2.2,in tea *** complementation assay showed that the expression of CsGDH2.1 in yeast gdh2 mutant rescued the theanine hypersensitivity and hyperaccumulation of this *** localization and tissue-specific expression showed CsGDH2.1 localized in the mitochondria and highly expressed in young ***,CsGDH2.1 expression was low in early spring,and increased significantly in late spring,in the new shoots of tea *** results all support the idea that CsGDH2.1 regulates theanine accumulation in the new ***,the in vitro enzyme assay showed that CsGDH2.1 had glutamate catabolic activity,and knockdown of CsGDH2.1 expression increased glutamate and theanine accumulation in the new shoots of tea *** findings suggested that CsGDH2.1-mediated glutamate catabolism negatively regulates theanine accumulation in the new shoots in late spring,and provides a functional gene for improving late-spring green tea quality.

关键词： spring infusion finding

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Suppression of Cs FAD3 in a JA-dependent manner,but not through the SA pathway,impairs drought stress tolerance in tea

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Journal of Integrative Agriculture 2024年第11期23卷 3737-3750页

作者： Na Chang Xiaotian Pi Ziwen Zhou Yeyun Li Xianchen Zhang state key laboratory of tea Plant biology and utilization Anhui Agricultural UniversityHefei 230036China

The growth and yield of tea plants are seriously limited by drought *** acid desaturases(FADs)contribute to the mediation of membrane fluidity in response to different stresses,although the role ofω-3 FAD(Omega-3fatty acid desaturase)-mediated damage induced by drought stress in tea plants is poorly *** this study,drought stress significantly promoted the synthesis of C18:3(linolenic acid)and the expression level of *** experiments further demonstrated that CsFAD3 can convert C18:2 to C18:3,and that the 35S:GFP-CsFAD3fusion protein was localized in the endoplasmic reticulum of Nicotiana benthamiana ***3-silenced tea leaves exhibited poor drought tolerance,with a lower F_(v)/F_(m)and a higher malondialdehyde(MDA)content than the control ***,transgenic 35S:CsFAD3 Arabidopsis plants showed the opposite *** addition,the jasmonic acid(JA)content and the expression levels of CsLOX2,CsLOX4,CsAOS,CsAOC3 and CsOPR2 were significantly reduced in CsFAD3-silenced leaves under drought ***,no substantial difference in the salicylic acid(SA)content was detected under normal or drought *** analysis of Atcoi1(JA receptor)or Atnpr1(SA receptor)mutant Arabidopsis plants in 35S:CsFAD3 backgrounds further revealed that knockout of Atcoi1impaired the drought-tolerant phenotypes of CsFAD3 overexpression ***,this study demonstrated that CsFAD3 plays a crucial role in drought tolerance by mediating JA pathways.

关键词： tea plants CsFAD3 drought stress jasmonic acid

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Functional diversity of subgroup 5 R2R3-MYBs promoting proanthocyanidin biosynthesis and their key residues and motifs in tea plant

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Horticulture Research 2023年第8期10卷 216-230页

作者： Tianming Jiao Yipeng Huang Ying-Ling Wu Ting Jiang Tongtong Li Yanzhuo Liu Yvchen Liu Yunyun Han Yajun Liu Xiaolan Jiang Liping Gao Tao Xia state key laboratory of tea Plant biology and utilization/key laboratory of tea biology and tea Processing of Ministry of Agriculture/Anhui Provincial laboratory of tea Plant biology and utilization Anhui Agricultural UniversityWest 130 Changjiang RoadHefei 230036 AnhuiChina School of Life Science Anhui Agricultural UniversityHefei 230036AnhuiChina

The tea plant(Camellia sinensis)is rich in polyphenolic ***,flavan-3-ols and proanthocyanidins(PAs)are essential for the flavor and disease-resistance property of tea *** fifth subgroup of R2R3-MYB transcription factors comprises the primary activators of PA *** study showed that subgroup 5 R2R3-MYBs in tea plants contained at least nine genes belonging to the TT2,MYB5,and MYBPA ***-rich plants showed an expansion in the number of subgroup 5 R2R3-MYB genes compared with other dicotyledonous and monocot *** MYBPA-type genes of tea plant were slightly ***–PCR analysis and GUS staining analysis of promoter activity under a series of treatments revealed the differential responses of CsMYB5s to biotic and abiotic *** particular,CsMYB5a,CsMYB5b,and CsMYB5e responded to high-intensity light,high temperature,MeJA,and mechanical wounding,whereas CsMYB5f and CsMYB5g were only induced by *** genetic transformation systems(***,Nicotiana tabacum,and Arabidopsis thaliana)were used to verify the biological function of *** results show that CsMYB5a,CsMYB5b,and CsMYB5e could promote the gene expression of CsLAR and ***,CsMYB5f and CsMYB5g could only upregulate the gene expression of CsLAR but not CsANR.A series of site-directed mutation and domain-swapping experiments were used to verify functional domains and key amino acids of CsMYB5s responsible for the regulation of PA *** study aimed to provide insight into the induced expression and functional diversity model of PA biosynthesis regulation in tea plants.

关键词： plant wound residues

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Development of synthetic volatile attractant for male Ectropis obliqua moths

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Journal of Integrative Agriculture 2016年第7期15卷 1532-1539页

作者： SUN Xiao-ling LI Xi-wang XIN Zhao-jun HAN Juan-juan RAN Wei LEI Shu tea Research Institute Chinese Academy of Agricultural Sciences key laboratory of tea biology and Resources utilization Ministry of Agriculture

The tea geometridEctropis obliquais one of the most serious leaf-feeding insect pests in tea （Camelia sinensis） in East Asia. Although several volatile chemicals emitted from tea plants have been reported to be attractive toE. obliqua moths, no synthetic attractants for E. obliqua moths have been developed. By measuring the behavioral responses of the moth to a series of chemicals in the lab, we found that a blend containing a ternary mixture containing （Z）-3-hexenal, （Z）-3-hexenyl hexanoate and benzyl alcohol clearly attracted toE. obliqua moths of both sex and that （Z）-3-hexenyl acetate could enhance the attractiveness of the ternary blend. Moreover, we found that the volatiles emitted from the plant-E. obliqua larva com-plex have the same attractiveness as： 1） the blend of volatiles containing the ternary mixture and 2） the blend containing （Z）-3-hexenyl acetate plus the ternary mixture to both male and female moths. In a ifeld bioassay, more male moths were observed on traps that were baited with the blend containing （Z）-3-hexenyl acetate plus the ternary mixture than on control traps. Our study raises the tantalizing possibility that synthetic blends could be deployed as attractants for pests in the ifeld.

关键词： attractant synthetic volatile blends Ectropis obliqua moth tea plantation

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Maintenance of mesophyll potassium and regulation of plasma membrane H^+-ATPase are associated with physiological responses of tea plants to drought and subsequent rehydration

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The Crop Journal 2018年第6期6卷 611-620页

作者： Xianchen Zhang Honghong Wu Linmu Chen Linlin Liu Xiaochun Wan state key laboratory of tea Plant biology and utilization Anhui Agricultural University Department of Botany and Plant Sciences University of California

Drought stress is one of the main factors limiting yield in tea plants. The plant cell's ability to preserve K^+homeostasis is an important strategy for coping with drought stress. Plasma membrane H^+-ATPase in the mesophyll cell is important for maintaining membrane potential to regulate K^+transmembrane transport. However, no research to date has investigated the possible relationship between plasma membrane H^+-ATPase and mesophyll K^+retention in tea plants under drought and subsequent rehydration conditions. In our experiment, drought stress inhibited plasma membrane H^+-ATPase activities and induced net H^+influx, leading to membrane potential depolarization and inducing a massive K^+efflux in tea plant mesophyll cells. Subsequent rehydration increased plasma membrane H^+-ATPase activity and induced net H^+efflux, leading to membrane potential hyperpolarization and thus lowering K^+loss. A first downregulated and then upregulated plasma membrane H^+-ATPase protein expression level was also observed under drought and subsequent rehydration treatment, a finding in agreement with the change of measured plasma membrane H^+-ATPase activities. Taken together, our results suggest that maintenance of mesophyll K^+in tea plants under drought and rehydration is associated with regulation of plasma membrane H^+-ATPase activity.

关键词： Drought stress H^+-ATPase Membrane potential Potassium Rehydration

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Efficient iron plaque formation on tea(Camellia sinensis) roots contributes to acidic stress tolerance

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Journal of Integrative Plant biology 2019年第2期61卷 155-167页

作者： Xianchen Zhang Honghong Wu Lingmu Chen Yeyun Li Xiaochun Wan state key laboratory of tea Plant biology and utilization Anhui Agricultural University Department of Botany and Plant Sciences University of California

tea plants grow in acidic soil, but to date, their intrinsic mechanisms of acidic stress tolerance have not been elucidated. Here, we assessed the tea plant response to growth on NHt4 nutrient media having different p H and iron levels. When grown in standard NHt4 nutrient solution(iron insufficient, 0.35 mg Là1 Fe2t), tea roots exhibited significantly lower nitrogen accumulation, plasma membrane Ht-ATPase activity, and protein levels; net Htefflux was lower at pH 4.0 and 5.0 than at pH 6.0. Addition of30 mg Là1 Fe2t(iron sufficient, mimicking normal soil Fe2tconcentrations) to the NHt4 nutrient solution led to more efficient iron plaque formation on roots and increased root plasma membrane Ht-ATPase levels and activities at p H 4.0 eland 5.0, compared to the p H 6.0 condition. Furthermore,plants grown at pH 4.0 and 5.0, with sufficient iron,exhibited significantly higher nitrogen accumulation than those grown at pH 6.0. Together, these results support the hypothesis that efficient iron plaque formation, on tea roots, is important for acidic stress tolerance. Furthermore,our findings establish that efficient iron plaque formation is linked to increased levels and activities of the tea root plasma membrane Ht-ATPase, under low pH conditions.

关键词： ATPase PM Efficient iron plaque formation on tea Figure Fe

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Camellia sinensis CsMYB4a participates in regulation of stamen growth by interaction with auxin signaling transduction repressor CsAUX/IAA4

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The Crop Journal 2024年第1期12卷 188-201页

作者： Guoliang Ma Mingzhuo Li Yingling Wu Changjuan Jiang Yifan Chen Dawei Xing Yue Zhao Yajun Liu Xiaolan Jiang Tao Xia Liping Gao School of Life Science Anhui Agricultural UniversityHefei 230036AnhuiChina Plant for Human Health Institute Department of Plant and Microbial BiologyNorth Carolina State UniversityKannapolisNC 28081USA state key laboratory of tea Plant biology and utilization/key laboratory of tea biology and tea Processing of Ministry of Agriculture/Anhui Provincial laboratory of tea Plant biology and utilization Anhui Agricultural UniversityHefei 230036AnhuiChina

Subgroup 4(Sg4)members of the R2R3-MYB are generally known as negative regulators of the phenylpropanoid pathway in *** previous research showed that a R2R3-MYB Sg4 member from Camellia sinensis(CsMYB4a)inhibits expression of some genes in the phenylpropanoid pathway,but its physiological function in the tea plant remained ***,CsMYB4a was found to be highly expressed in anther and filaments,and participated in regulating filament *** analysis and exogenous auxin treatment showed that the target of CsMYB4a might be the auxin signal ***/indole-3-acetic acid 4(AUX/IAA4),a repressor in auxin signal transduction,was detected from a yeast two-hybrid screen using CsMYB4a as *** silencing assays showed that both CsIAA4 and CsMYB4a regulate filament *** plants overexpressing CsIAA4 were insensitive to exogenous a-NAA,consistent with overexpression of ***-protein interaction experiments revealed that CsMYB4a interacts with N-terminal of CsIAA4 to prevent CsIAA4 *** out of the endogenous NtIAA4 gene,a CsIAA4 homolog,in tobacco alleviated filament growth inhibition and a-NAA insensitivity in plants overexpressing *** results strongly suggest that CsMYB4a works synergistically with CsIAA4 and participates in regulation of the auxin pathway in stamen.

关键词： AUX/IAA4 Auxin signaling CsMYB4a Subgroup 4 R2R3-MYB

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