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Regulation of developmental and environmental signaling by interaction between microtubules and membranes in plant cells

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Protein & Cell 2016年第2期7卷 81-88页

作者： Qun Zhang Wenhua Zhang College of Life Sciences State Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing AgriculturalUniversity Nanjing 210095 China

Cell division and expansion require the ordered arrangement of microtubules, which are subject to spatial and temporal modifications by developmental and environmental factors. Understanding how signals translate to changes in cortical microtubule organization is of fundamental importance. A defining feature of the cortical microtubule array is its association with the plasma membrane; modules of the plasma membrane are thought to play important roles in the mediation of microtubule organization. In this review, we highlight advances in research on the regulation of cortical microtubule organization by membrane-associated and membrane-tethered proteins and lipids in response to phytohormones and stress. The transmembrane kinase receptor Rho-like guanosine triphosphatase, phospholipase D, phosphatidic acid, and phosphoinositides are discussed with a focus on their roles in microtubule organization.

关键词： abiotic stresses cortical microtubule,lipids plasma membrane

B-box proteins:Pivotal players in light-mediated development in plants^FA

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Journal of Integrative Plant Biology 2020年第9期62卷 1293-1309页

作者： Zhaoqing Song Yeting Bian Jiujie Liu Yuting Sun Dongqing Xu state key laboratory of crop genetics and germplasm enhancement College of AgricultureNanjing Agricultural UniversityNanjing 210095China

Light signals mediate a number of physio-logical and developmental processes in plants, such as flowering, photomorphogenesis, and pigment accu-mulation. Emerging evidence has revealed that a group of B-box proteins (BBXs) function as central players in these light-mediated developmental processes. B-box proteins are a class of zinc-coordinated transcription factors or regulators that not only directly mediate the transcription of target genes but also interact with various other factors to create a complex regulatory network involved in the precise control of plant growth and development. This review summarizes and highlights the recent findings concerning the critical regulatory functions of BBXs in photoperiodic flow-ering, light signal transduction and light-induced pigment accumulation and their molecular modes of action at the transcriptional and post-translational levels in plants.

关键词： light precise concerning

Optimizing the Bacillus thuringiensis(Bt)protein concentration in cotton:Coordinated application of exogenous amino acids and EDTA to reduce spatiotemporal variability in boll and leaf toxins

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Journal of Integrative Agriculture 2024年第10期23卷 3419-3436页

作者： Zhenyu Liu Shu Dong Yuting Liu Hanjia Li Fuqin Zhou Junfeng Ding Zixu Zhao Yinglong Chen Xiang Zhang Yuan Chen Dehua Chen Jiangsu key laboratory of crop genetics and Physiology/Co-Innovation Center for Modern Production Technology of Graincrops Yangzhou UniversityYangzhou 225009China The UWA Institute of Agriculture School of Agriculture and EnvironmentThe University of Western AustraliaPerth 6009WAAustralia

During the boll formation stage,cotton bolls exhibit the lowest expression of Bacillus thuringiensis(Bt)insecticidal *** to insects varies notably among different organs,which poses challenges for controlling cotton ***,an experimental strategy was designed in the 2020-2021 cotton growing season to coordinate the enhancement of protein synthesis and the attenuation of *** Bt cultivars of Gossypium hirsutum,namely the hybrid Sikang 3 and the conventional Sikang 1,were used as test *** treatments were applied at the peak flowering period:CK(the control),T1(amino acids),and T2(amino acids and EDTA).The results show that,in comparison to the CK group,the Bt protein contents were significantly increased in both cotton bolls and their subtending leaves under the T1 and T2 *** maximum levels of increase observed were 67.5%in cotton bolls and 21.7%in ***,the disparity in Bt protein content between cotton bolls and their subtending leaves notably decreased by 31.2%.Correlation analysis suggested that the primary physiological mechanisms for augmenting Bt protein content involve increased protein synthesis and reduced protein catabolism,which are independent of Bt gene expression *** regression and path analysis revealed that elevating the soluble protein content and transaminase activity,while reducing the catabolic enzyme activities,are instrumental in enhancing the Bt protein ***,the coordinated application of amino acids and EDTA emerges as a strategy that can improve the overall resistance of Bt cotton and mitigate the spatiotemporal variations in Bt toxin concentrations in both cotton bolls and leaves.

关键词： Gossypium hirsutum Bt cotton insecticidal protein bolls and their subtending leaves nitrogen metabolism

Linkage graph analysis:A linkage-group-based QTL synthesis analysis approach

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Chinese Science Bulletin 2011年第11期56卷 1092-1099页

作者： KUANG FengLei WANG Xia ZHOU Ling ZHANG YuanMing state key laboratory of crop genetics and germplasm enhancement Nanjing Agricultural University Nanjing 210095 China

Consensus quantitative trait loci (QTL) in meta-analysis of multiple independent QTL mapping experiments provides a strong foundation for marker-assisted selection and gene ***,meta-analysis suffers from the lack of available genomic information and the results vary when different reference linkage maps are ***,to overcome these limitations,we propose a linkage-group-based QTL synthesis analysis approach that we have named linkage graph ***,a graph model is constructed from derived linkage ***,an unsupervised classification approach is used to obtain marker intervals with co-segregating patterns among multiple ***,a frequent itemset mining technique is used to identify the markers (or intervals) closely linked to the *** proposed method was validated by one Monte Carlo simulation study and by real data analysis of cotton *** major advantages of the new method are:(i) A reference linkage group is not required;(ii) the effect of the initial QTL is reduced because false QTLs can be detected and excluded from the *** ability to reliably identify the markers associated with a true QTL is valuable in crop breeding.

关键词： QTL效应综合分析方法连锁群连锁图基础分子标记辅助选择基因组信息数量性状位点

Telomere-to-telomere genome of the allotetraploid legume Sesbania cannabina reveals transposon-driven subgenome divergence and mechanisms of alkaline stress tolerance

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Science China(Life Sciences) 2024年第1期67卷 149-160页

作者： Haofei Luo Xiaofei Wang Changqing You Xuedan Wu Duofeng Pan Zhiyao Lv Tong Li Dongmei Zhang Zhongbao Shen Xiaodong Zhang Guodao Liu Kaixuan He Qingtong Ye Yajun Jia Qinghua Zhao Xian Deng Xiaofeng Cao Xianwei Song Gai Huang state key laboratory of Plant Genomics and National Center for Plant Gene Research Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China Hainan Yazhou Bay Seed Lab Sanya 572025China University of Chinese Academy of Sciences Beijing 100049China Institute of Forage and Grassland Sciences Heilongjiang Academy of Agricultural SciencesHarbin 150086China Shandong Academy of Agricultural Sciences Jinan 250100China National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land Dongying 257345China Shandong Green Manure Ecological Technology Co. LtdDongying 257345China state key laboratory of Tropical crop Breeding Chinese Academy of Tropical Agricultural SciencesHaikou 571101China

Alkaline soils pose an increasing problem for agriculture worldwide,but using stress-tolerant plants as green manure can improve marginal ***,we show that the legume Sesbania cannabina is very tolerant to alkaline conditions and,when used as a green manure,substantially improves alkaline *** understand genome evolution and the mechanisms of stress tolerance in this allotetraploid legume,we generated the first telomere-to-telomere genome assembly of *** spanning~2,087 *** assembly included all centromeric regions,which contain centromeric satellite repeats,and complete chromosome ends with telomeric *** genome analysis distinguished A and B subgenomes,which diverged approximately 7.9 million years *** genomic analysis revealed that the chromosome homoeologs underwent large-scale inversion events(>10 Mb)and a significant,transposon-driven size expansion of the chromosome 5A *** further identified four specific alkali-induced phosphate transporter genes in ***;these may function in alkali tolerance by relieving the deficiency in available phosphorus in alkaline *** work highlights the significance of *** as a green tool to improve marginal lands and sheds light on subgenome evolution and adaptation to alkaline soils.

关键词： Sesbania cannabina alkaline stress telomere-to-telomere genome legume green manure phosphate transporter allotetraploid

Molecular Cloning,Expression Analysis and Localization of Exo70A1 Related to Self Incompatibility in Non-Heading Chinese Cabbage(Brassica campestris ***)

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Journal of Integrative Agriculture 2013年第12期12卷 2149-2156页

作者： WANG Li GE Ting-ting PENG Hai-tao WANG Cheng LIU Tong-kun HOU Xi-lin LI Ying Horticultural Department Nanjing Agricultural University state key laboratory of crop genetics and germplasm enhancement key laboratory of Southern Vegetable crop Genetic Improvement Ministry of Agriculture

The exocyst is a conserved protein complex,and required for vesicles tethering,fusion and polarized ***70A1,the exocyst subunit,is essential for assembly of the exocyst *** better understand potential roles of Exo70A1 in non-heading Chinese cabbage（Brassica campestris ***）,we obtained the full-length cDNA of Exo70A1 gene,which consisted of 1 917 bp and encoded a protein of 638 amino *** showed BcExo70A1 shared 94.9% identity with Brassica oleracea ***（AEI26267.1）,and clustered into a same group with other homologues in *** *** and Brassica *** localization analysis showed BcExo70A1 was localized to punctate structures in cytosol of onion epithelial *** showed that BcExo70A1 was widely presented in stamens,young stems,petals,unpollinated pistils,roots and leaves of self compatible and incompatible *** transcripts of BcExo70A1 in non- heading Chinese cabbage declined during initial 1.5 h after incompatible pollination,while an opposite trend was presented after compatible *** study reveals that BcExo70A1 could play essential roles in plant growth and development,and is related to the rejection of self pollen in non-heading Chinese cabbage.

关键词： Exo70A1 gene expression subcellular localization self-incompatibility non-heading Chinese cabbage

Plant regeneration in the new era:from molecular mechanisms to biotechnology applications

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Science China(Life Sciences) 2024年第7期67卷 1338-1367页

作者： Chunli Chen Yuxin Hu Momoko Ikeuchi Yuling Jiao Kalika Prasad Ying Hua Su Jun Xiao Lin Xu Weibing Yang Zhong Zhao Wenkun Zhou Yun Zhou Jian Gao Jia-Wei Wang National key laboratory for germplasm Innovation&Utilization of Horticultural crops Hubei Hongshan LaboratoryHuazhong Agricultural UniversityWuhan 430070China College of Life Science and Technology Huazhong Agricultural UniversityWuhan 430070China key laboratory of Plant Molecular Physiology Institute of BotanyChinese Academy of Sciences(CAS)China National Botanical GardenBeijing 100093China Division of Biological Sciences Graduate School of Science and TechnologyNara Institute of Science and TechnologyNara 630-0192Japan state key laboratory of Protein and Plant Gene Research School of Life SciencesPeking UniversityBeijing 100871China Peking-Tsinghua Center for Life Sciences Center for Quantitative BiologyAcademy for Advanced Interdisciplinary StudiesPeking UniversityBeijing 100871China Indian Institute of Science Education and Research Pune 411008IndiaThiruvananthapuram 695551India state key laboratory of Wheat Improvement College of Life SciencesShandong Agricultural UniversityTai’an 271018China Sino-German Joint Research Center on Agricultural Biology Shandong Agricultural UniversityTai’an 271018China key laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental Biology(IGDB)CASBeijing 100101China CAS-JIC Centre of Excellence for Plant and Microbial Science(CEPAMS) IGDBCASBeijing 100101China National key laboratory of Plant Molecular genetics CEMPSInstitute of Plant Physiology and Ecology(SIPPE)CASShanghai 200032China CEPAMS SIPPECASShanghai 200032China Hefei National laboratory for Physical Sciences at the Microscale CEMPSSchool of Life SciencesDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230027China state key laboratory of Plant Environmental Resilience College of Biological SciencesChina Agricultural UniversityBeijing 100193China Department of Botany and Plant Pathology and Center for Plant Biology Purdue UniversityWest Lafayette 47907USA School of Life Science and Technology Shang

Plants or tissues can be regenerated through various *** animal regeneration,cell totipotency and pluripotency are the molecular basis of plant *** systematic studies on Arabidopsis thaliana gradually unravel the fundamental mechanisms and principles underlying plant ***,plant hormones,cell division,epigenetic remodeling,and transcription factors play crucial roles in reprogramming somatic cells and reestablishing meristematic *** research on basal non-vascular plants and monocot crops has revealed that plant regeneration differs among species,with various plant species using distinct mechanisms and displaying significant differences in regenerative *** multi-omics studies at the single-cell level,tracking plant regeneration processes in real-time,and deciphering the natural variation in regenerative capacity will ultimately help understand the essence of plant regeneration,improve crop regeneration efficiency,and contribute to future crop design.

关键词： apical meristem model species regeneration phytohormone plant cell reprogramming somatic embryogenesis

Functional Analysis of Autophagy Genes via Agrobacterium-Mediated Transformation in the Vascular Wilt Fungus Verticillium dahliae

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Journal of genetics and Genomics 2013年第8期40卷 421-431页

作者： Lei Zhou Jun Zhao Wangzhen Guo Tianzhen Zhang National key laboratory of crop genetics and germplasm enhancement Cotton Research InstituteNanjing Agricultural University

Autophagy is a widely conserved intracellular process for degradation and recycling of proteins, organelles and cytoplasm in eukaryotic organisms and is now emerging as an important process in tbliar infection by many plant pathogenic fungi. However, the role of autophagy in soil-borne fungal physiology and infection biology is poorly understood. Here, we report the establishment of an Agro- bacterium tumefaciens-mediated transformation （ATMT） system and its application to investigate two autophagy genes, VdATG8 and VdATG12, by means of targeted gene replacement and complementation. Transformation of a cotton-infecting Verticillium dahliae strain Vd8 with a novel binary vector pCOM led to the production of 384 geneticin-resistant translbnnants per 1 × 10^4 conidia. V. dahliae mutants lacking either VdATG8 or VdATGI2 exhibited reduced conidiation and impaired aerial hyphae production. Disease development on Arabidopsis plants was slightly delayed when inoculated with VdATG8 or VdATG12 gene deletion mutants, compared with the wild- type and gene complemented strains. Surprisingly, in vitro inoculation with unimpaired roots revealed that the abilities of root invasion were not affected in gene deletion mutants. These results indicate that autophagy is necessary for aerial hyphae development and plant colonization but not for root infection in E dahliae.

关键词： Verticillium dahliae Autophagy: ATMT: Targeted gene replacement Pathogenicity

Sequence Divergence of Microsatellites and Phylogeny Analysis in Tetraploid Cotton Species and Their Putative Diploid Ancestors

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Journal of Integrative Plant Biology 2005年第12期47卷 1418-1430页

作者： Wang-Zhen GUO Dong FANG Wen-Duo YU Tian-Zhen ZHANG state key laboratory of crop genetics and germplasm enhancement Cotton Research Institute Nanjing Agricultural University Nanjing 210095 China

To determine the level of microsatellite sequence differences and to use the information to construct a phylogenetic relationship for cultivated tetraploid cotton （Gossypium spp.） species and their putative diploid ancestors, 10 genome-derived microsatellite primer pairs were used to amplify eight species, including two tetraploid and six diploid species, in Gossypium. A total of 92 unique amplicons were resolved using polyacrylamide gel electrophoresis. Each amplicon was cloned, sequenced, and analyzed using standard phylogenetic software. Allelic diversities were caused mostly by changes in the number of simple sequence repeat （SSR） motif repeats and only a small proportion resulted from interruption of the SSR motif within the locus for the same genome. The frequency of base substitutions was 0.5%-1.0% in different genomes, with only few indels found. Based on the combined 10 SSR flanking sequence data, the homology of A-genome diploid species averaged 98.9%, even though most of the amplicons were of the same size, and the sequence homology between G. gossypioides （Ulbr.） Standl. and three other D-genome species （G. raimondii Ulbr., G. davidsonii Kell., and G. thurberi Tod.） was 98.5%, 98.6%, and 98.5%, respectively. Phylogenetic trees of the two allotetraploid species and their putative diploid progenitors showed that homoelogous sequences from the A- and D-subgenome were still present in the polyploid subgenomes and they evolved independently. Meanwhile, homoelogous sequence interaction that duplicated loci in the polyploid subgenomes became phylogenetic sisters was also found in the evolutionary history of tetraploid cotton species. The results of the present study suggest that evaluation of SSR variation at the sequence level can be effective in exploring the evolutionary relationships among Gossypuim species.

关键词： diploid Gossypium microsatellite phylogenetic tree simple sequence repeat (SSR) tetraploid.