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Comparison of the signaling pathways of wing dimorphism regulated by biotic and abiotic stress in the brown planthopper

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Insect Science 2023年第4期30卷 1046-1062页

作者： Jing-Xiang Chen Wan-Xue Li Qin Su Jun Lyu Yi-Bing Zhang Wen-Qing Zha State Key Laboratory of Biocontrol and School of Life Sciences Sun Yat-sen UniversityGuangzhouChina

Wing polymorphism is an evolutionary trait that is widely present in various insects and provides a model system for studying the evolutionary significance of insect dispersal.The brown planthopper(BPH,Nilaparvata lugens)can alter its wing morphs un-der biotic and abiotic stress.However,whether differential signaling pathways are induced by the 2 types of stress remain largely unknown.Here,we screened a number of candidate genes through weighted gene co-expression network analysis(WGCNA)and found that ornithine decarboxylase(NIODC),a key enzyme in the synthesis of polyamines,was as-sociated with wing differentiation in BPH and mainly responded to abiotic stress stimuli.We analyzed the Kyoto Encyclopedia of Genes and Genomes enrichment pathways of dif-ferentially expressed genes under the 2 stresses by transcriptomic comparison,and found that biotic stress mainly influenced insulin-related signaling pathways while abiotic stress mainly influenced hormone-related pathways.Moreover,we found that insulin receptor 1(NllnRI)may regulate wing differentiation of BPH by responding to both biotic and abiotic stress,but NllnR2 only responded to biotic stress.Similarly,the juvenile hormone epoxide hydrolase associated with juvenile hormone degradation and NIODC may regu-late wing differentiation mainly through abiotic stress.A model based on the genes and stresses to modulate the wing dimorphism of BPH was proposed.These findings present a comprehensive molecular mechanism for wing polymorphism in BPH induced by biotic and abiotic stress.

关键词： insulin juvenile hormone Nilaparvata lugens ornithine decarboxylase stress wing dimorphism

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两种尺度的生命之树:从物种到细胞

Science Bulletin 2023年第21期68卷 2515-2518页

作者：邓善俊贺雄雷 state key laboratory of biocontrol School of Life SciencesSun Yat-sen UniversityGuangzhou 510275China

All species on the Earth can trace their origins back to a common ancestor,and their evolutionary history can be illustrated as a tree-like structure.The root of the tree represents the shared ancestor,while the bifur... 详细信息

关键词： structure illustrated tree

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Functional characterization of piggyBac-like elements from Nilaparvata lugens(St?l)(Hemiptera:Delphacidae)

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2022年第6期23卷 515-527页

作者： Jun LYU Qin SU Jinhui LIU Lin CHEN Jiawei SUN Wenqing ZHANG state key laboratory of biocontrol School of Life SciencesSun Yat-sen UniversityGuangzhou 510275China

Piggy Bac is a transposable DNA element originally discovered in the cabbage looper moth(Trichoplusia ni).The T.ni piggyBac transposon can introduce exogenous fragments into a genome,constructing a transgenic organism.Nevertheless,the comprehensive analysis of endogenous piggyBac-like elements(PLEs)is important before using piggyBac,because they may influence the genetic stability of transgenic lines.Herein,we conducted a genome-wide analysis of PLEs in the brown planthopper(BPH)Nilaparvata lugens(St?l)(Hemiptera:Delphacidae),and identified a total of 28 PLE sequences.All N.lugens piggy Bac-like elements(NlPLEs)were present as multiple copies in the genome of BPH.Among the identified NlPLEs,NlPLE25 had the highest copy number and it was distributed on five chromosomes.The full length of NlPLE25 consisted of terminal inverted repeats and sub-terminal inverted repeats at both terminals,as well as a single open reading frame transposase encoding546 amino acids.Furthermore,NlPLE25 transposase caused precise excision and transposition in cultured insect cells and also restored the original TTAA target sequence after excision.A cross-recognition between the NlPLE25 transposon and the piggy Bac transposon was also revealed in this study.These findings provide useful information for the construction of transgenic insect lines.

关键词： Nilaparvata lugens piggyBac-like elements Transposon NlPLE25

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Decoupling gene functions from knockout effects by evolutionary analyses

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National Science Review 2020年第7期7卷 1169-1180页

作者： Li Liu Mengdi Liu Di Zhang Shanjun Deng Piaopiao Chen Jing Yang Yunhan Xie Xionglei He state key laboratory of biocontrol School of Life Sciences Sun Yat-sen University

Genic functions have long been confounded by pleiotropic mutational effects. To understand such genetic effects, we examine HAP4, a well-studied transcription factor in Saccharomyces cerevisiae that functions by forming a tetramer with HAP2, HAP3 and HAP5. Deletion of HAP4 results in highly pleiotropic gene expression responses, some of which are clustered in related cellular processes(clustered effects) while most are distributed randomly across diverse cellular processes(distributed effects). Strikingly, the distributed effects that account for much of HAP4 pleiotropy tend to be non-heritable in a population,suggesting they have few evolutionary consequences. Indeed, these effects are poorly conserved in closely related yeasts. We further show substantial overlaps of clustered effects, but not distributed effects, among the four genes encoding the HAP2/3/4/5 tetramer. This pattern holds for other biochemically characterized yeast protein complexes or metabolic pathways. Examination of a set of cell morphological traits of the deletion lines yields consistent results. Hence, only some deletion effects of a gene support related biochemical understandings with the rest being often pleiotropic and evolutionarily decoupled from the gene’s normal functions. This study suggests a new framework for reverse genetic analysis.

关键词： pleiotropy mutational effects heritability reverse genetics gene function

Heightened protein-translation activities in mammalian cells and the disease/treatment implications

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National Science Review 2020年第12期7卷 1851-1855页

作者： Chung-I Wu Haijun Wen state key laboratory of biocontrol School of Life SciencesSun Yat-sen University

After cells divide,the new cells have to synthesize all the necessary cellular components in time for the next division.There is a lower bound of time required for cells to double their contents.For mammalian cells,th... 详细信息

关键词： MYELOID-LEUKEMIA OMACETAXINE MEPESUCCINATE HOMOHARRINGTONINE INHIBITION CANCER HARRINGTONINE HETEROGENEITY EVOLUTION

Development of a high-fidelity Cas9-dependent adenine base editor (ABE) system for genome editing with high-fidelity Cas9 variants

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Genes & Diseases 2023年第3期10卷 705-707页

作者： Ruisha Hong Xionglei He state key laboratory of biocontrol School of Life SciencesSun Yat-Sen UniversityGuangzhouGuangdong 510275China

One of the main reasons that hinders the application of base editors in the clinic is the trade-off between editing efficiency and editing fidelity.The off-target base editing includes Cas9-independent and Cas9-dependent manners.1 The Cas9-independent off-target results from the intrinsic deamination activity of the deaminase domain of a base editor,which affects random RNA or DNA molecules.

关键词： base fidelity adenine

Two decades of suspect evidence for adaptive molecular evolution——negative selection confounding positive-selection signals

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National Science Review 2022年第5期9卷 129-141页

作者： Qipian Chen Hao Yang Xiao Feng Qingjian Chen Suhua Shi Chung-Ⅰ Wu Ziwen He state key laboratory of biocontrol Guangdong Key Laboratory of Plant Resources School of Life SciencesSouthern Marine Science and Engineering Guangdong Laboratory(Zhuhai) Sun Yat-sen University

There has been a large literature in the last two decades affirming adaptive DNA sequence evolution between species.The main lines of evidence are from(i) the McDonald-Kreitman(MK) test,which compares divergence and polymorphism data,and(ii) the phylogenetic analysis by maximum likelihood(PAML) test,which analyzes multispecies divergence data.Here,we apply these two tests concurrently to genomic data of Drosophila and Arabidopsis.To our surprise,the> 100 genes identified by the two tests do not overlap beyond random expectation.Because the non-concordance could be due to low powers leading to high false negatives,we merge every 20-30 genes into a ’supergene’.At the supergene level,the power of detection is large but the calls still do not overlap.We rule out methodological reasons for the non-concordance.In particular,extensive simulations fail to find scenarios whereby positive selection can only be detected by either MK or PAML,but not both.Since molecular evolution is governed by positive and negative selection concurrently,a fundamental assumption for estimating one of these(say,positive selection) is that the other is constant.However,in a broad survey of primates,birds,Drosophila and Arabidopsis,we found that negative selection rarely stays constant for long in evolution.As a consequence,the variation in negative selection is often misconstrued as a signal of positive selection.In conclusion,MK,PAML and any method that examines genomic sequence evolution has to explicitly address the variation in negative selection before estimating positive selection.In a companion study,we propose a possible path forward in two stages—first,by mapping out the changes in negative selection and then using this map to estimate positive selection.For now,the large literature on positive selection between species has to await reassessment.

关键词： molecular evolution neutral theory natural selection

Screening of Brown Planthopper Resistant miRNAs in Rice and Their Roles in Regulation of Brown Planthopper Fecundity

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Rice science 2022年第6期29卷 559-568,I0031-I0033页

作者： LÜJun LIU Jinhui CHEN Lin SUN Jiawei SU Qin LI Shihui YANG Jianhua ZHANG Wenqing state key laboratory of biocontrol School of Life SciencesSun Yat-sen UniversityGuangzhou 510275China

MicroRNAs(miRNAs)can participate in plant-insect interactions,which regulate plant defense networks.In this study,we analyzed the miRNA expression profiles of six rice varieties before and after brown planthopper(BPH)-feeding.We identified 45 differentially expressed miRNAs between BPH-susceptible and BPH-resistant rice varieties and 144 miRNAs that responded to BPH-feeding.Thus,miRNAs may be involved in multiple pathways regulating rice defense response against BPH.In addition,we found that the genetic history of rice varieties determined the regulation mode of the miRNA and affected the amounts,types,changing trends and response periods of miRNAs in response to BPH-feeding.To conclude,we scanned seven potential cross-kingdom miRNAs,of which miR5795 may target the vitellogenin gene in BPH,causing a 16.07%reduction in BPH oviposition.The results provide new miRNA information of rice-BPH interactions and BPH-resistant rice variety breeding.

关键词： Nilaparvata lugens insect-resistant rice miR5795 fecundity

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On the epicenter of COVID-19 and the origin of the pandemic strain

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National Science Review 2023年第4期10卷 21-23页

作者： Yongsen Ruan Haijun Wen Mei Hou Weiwei Zhai Shuhua Xu Xuemei Lu state key laboratory of biocontrol School of Life Sciences Sun Yat-sen University Institute of Zoology Chinese Academy of Sciences school of life sciences Fudan University state key laboratory of Genetic Resources and Evolution Yunnan key laboratory of Biodiversity Information Kunming Institute of Zoology Chinese Academy of Sciences

Ruan et al.(2022) published in National Science Review(NSR) an analysis that concludes two early centers in the spread of COVID-19 [1]. Since the strain(referred to as DG1111) that spread globally to cause the pandemi... 详细信息

关键词： SARS-COV-2