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Effects of thymol and carvacrol supplementation on intestinal integrity and immune responses of broiler chickens challenged with Clostridium perfringens

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Journal of animal science and Biotechnology 2016年第4期7卷 522-531页

作者： Encun Du Weiwei Wang Liping Gan Zhui Li Shuangshuang Guo Yuming Guo state key laboratory of animal nutrition College of Animal Science and TechnologyChina Agricultural University

Background： Necrotic enteritis caused by Clostfidium perffingens infection leads to serious economic losses in the global poultry production. In the present study, we investigated the protective effects of essential oils （EO, which contained 25 % thymol and 25 % carvacrol as active components） supplementation on growth performance, gut lesions, intestinal morphology, and immune responses of the broiler chickens infected with C. perfringens. A total of 448 1-day-old male broiler chicks were allocated into eight treatment groups following a 4 x 2 factorial arrangement with four dietary EO dosages （0, 60, 120, or 240 mg/kg） and two infection status （with or without C. perfringens challenge from d 14 to 20）. Results： The challenge did not impair the growth performance of birds, but induced gut lesions and increased crypt depth in the ileum （P ≤ 0.05）. It also down-regulated the claudin-1 and occludin mRNA expression （P ≤0.05）, up-regulated the mRNA expression of interleukin-113 （P≤ 0.05）, tended to increase the toll-like receptor （TLR） 2 mRNA expression （P 〈 0.10） in the ileum, and enhanced the mucosal secretory IgA production （P 〈 0.05）. In the challenged birds, dietary EO supplementation linearly alleviated the gut lesions and improved the ratio of villus height to crypt depth （P ≤0.05）, and the supplementation of 120 and 240 mg/kg EO increased the serum antibody titers against Newcastle disease virus （P≤ 0.05）. Regardless of challenge, the EO supplementation showed a tendency to linearly elevate the feed conversion efficiency between 14 and 28 d of age as well as the occludin mRNA expression （P〈 0.10）, and linearly inhibited the mRNA expression of TLR2 and tumor necrotic factor-o in the ileum （P≤ 0.05）. Conclusions： The dietary supplementation of EO could alleviate the intestinal injury by improving intestinal integrity and modulating immune responses in the C. perffingens-challenged broiler chickens.

关键词： Broiler chicken Clostridium peffringens Essential oils Immune response Intestinal integrity

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Effect of lactic acid bacteria inoculants on alfalfa(Medicago sativa L.) silage quality:assessment of degradation(in situ) and gas production(in vitro)

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Journal of Integrative Agriculture 2016年第12期15卷 2834-2841页

作者： LIU Ce LAI Yu-jiao LU Xiao-nan GUO Ping-ting LUO Hai-ling state key laboratory for animal nutrition College of Animal Science and TechnologyChina Agricultural University

Alfalfa （Medicago sativa） is difficult to ensile successfully because of the low content of moisture and water-soluble carbohydrates （WSC） in fresh alfalfa and the high buffering capacity in fresh alfalfa. Here, we conducted a study to evaluate the effects of three lactic acid bacteria （LAB） inoculants （Lactobacillus case/, lactobacillus plantarum, and Pediococcus pentosaceus） on silage quality, in sitE/ruminal degradability, and in vitro fermentation of alfalfa silage. The first cut of alfalfa was wilted, chopped, and randomly divided into four groups： the control （CON） and control mixed with three separate LAB inoculants （106 cfu g-1）. Simmental steers with a body weight of （452±18） kg and with installed rumen fistulas were prepared for in situ degradation and for in vitro gas production. LAB inoculants had a lower （P〈0.05） content of butyric acid than the CON group. Among them, the L. casei inoculated silage had a higher （P〈0.05） content of water-soluble carbohydrate （WSC） and a lower （P〈0.05） NH3-H content. The effective degradation （ED） of crude protein in LAB inoculation decreased （P〈0.05）, while the ED of acid detergent fiber increased （P〈0.05） in situ fermentation. The alfalfa silage with LAB inoculants produced more carbon dioxide （P〈0.05）. The NH3-H content of mixed incubation fluid in L. casei inoculated silage was lower （P〈0.05） compared with other groups. Therefore, this study showed that LAB inoculants could improve both ensiling quality and degradation. In particular, the L. casei inoculations exhibited better performance by limiting proteolysis during ensiling.

关键词： alfalfa silage LAB inoculants nylon-bag degradation gas production fermentation

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In vitro antibacterial activity of thymol and carvacrol and their effects on broiler chickens challenged with Clostridium perfringens

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Journal of animal science and Biotechnology 2016年第1期7卷 95-106页

作者： Encun Du Liping Gan Zhui Li Weiwei Wang Dan Liu Yuming Guo state key laboratory of animal nutrition College of Animal Science and TechnologyChina Agricultural University

Background： In the post-antibiotic era, essential oils （EO） are promising alternatives to growth-promoting antibiotics. The aim of the present study was to investigate the antibacterial activities of an EO product and its components thymol and carvacrol in vitro, and the efficacy of EO to control Clostridium perffingens challenge in broiler chickens. Results： The in vitro minimum inhibitory concentration assay showed strong antibacterial activity of the EO product, thymol, and carvacrol against pathogenic Escherichia col~, C. perffingens, and Salmonella strains, and weak activity towards beneficial Lactobacillus strains. Besides, an additive effect was observed between thymol and carvacrol. The in vivo study was carried out with 448 male broiler chicks following a 4 x 2 factorial arrangement to test the effects of EO supplementation （0, 60, 120, or 240 mg/kg EO in wheat-based diet）, pathogen challenge （with or without oral gavage of C. perfr/ngens from day 14 to day 20） and their interactions. Each treatment consisted of eight replicate pens （seven birds/pen）. The challenge led to macroscopic gut lesions, and resulted in a significant increase in ileal populations of C. perffingens and Eschefichia subgroup （P ≤ 0.05） on day 21. Dietary EO supplementation did not influence C. perfringens numbers, but linearly alleviated intestinal lesions on day 21 and 28 （P= 0.010 and 0.036, respectively）, and decreased Eschefichia populations in ileum with increased EO dosages （P = 0.027 and 0.071 for day 21 and 28, respectively）. For caecum, EO quadratically influenced Lactobacillus populations on day 21 （P = 0.002）, and linearly decreased the numbers of total bacteria and Eschefichio on day 28 （P = 0.026 and 0.060, respectively）. Mean thymol and carvacrol concentrations in the small intestine were 0.21 and 0.20 μg/g in intestinal digesta （wet weight）, respectively, for birds fed 60 mg/kg EO, and 0.80 and 0.71 μg/g, respectively, for birds fed 240 mg/kg EO. Conclusions： These resu

关键词： Thymol Carvacrol Essential oils Clostfidium perfringens Eschefichia coil Broiler chickens

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Effects of dietary tryptophan and stocking density on the performance,meat quality,and metabolic status of broilers

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Journal of animal science and Biotechnology 2015年第1期6卷 90-96页

作者： Bo Wang Zhizhi Min Jianmin Yuan Bingkun Zhang Yuming Guo state key laboratory of animal nutrition College of Animal Science and TechnologyChina Agricultural University

Background： Highly automated cage-rearing systems are becoming increasingly popular in China. However, a high stocking density can cause oxidative stress and decrease broiler performance. The tryptophan （TRP） derivative 5-hydroxytryptophan （5-HT） has been shown to preserve membrane fluidity in birds suffering from oxidative stress Therefore, this experiment was conducted to determine the effects of dietan/TRP supplementation on performance, breast meat quality and oxidative stress in broilers reared in cages with a high or low stocking density. Methods： Female Arbor Acres broilers （25-d-old, n = 144） were randomly allocated to 1 of 4 treatments. The birds were fed a diet based on corn, soybean meal, cottonseed meal and corn gluten meal containing either 0.18 or 0.27% TRP and were housed with stocking densities of 11 or 15.4 birds/m2 in a 2 x 2 factorial experiment. Broiler performance was evaluated from d 25 to 42. Eight birds from each treatment were slaughtered on d 42 and plasma and breast muscle samples were collected to measure biochemical indices. Results： A higher stocking density tended to be associated with reduced weight gain （P 〈 0.10）, and significantly increased plasma glutamic-pyruvic transaminase （GPT） activity （P 〈 0.001）. Increased dietary TRP significantly reduced the activities of lactic dehydrogenase and GPT while increasing total cholesterol in the plasma （P 〈 0.01）, reducing drip loss of breast muscle （P 〈 0.10） and improving feed efficiency （P 〈 0.10）. Conclusions： An increase in dietary TRP, ].S-fold higher than the standard supplementation level, can alleviate oxidative stress as well as improve welfare and feed efficiency in broilers reared in cages with a high stocking density.

关键词： Broiler Meat quality Performance Stocking density Tryptophan

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Effects of Lactobacillus acidophilus on the growth performance and intestinal health of broilers challenged with Clostridium perfringens

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Journal of animal science and Biotechnology 2018年第3期9卷 689-698页

作者： Zhui Li Weiwei Wang Dan Liu Yuming Guo state key laboratory of animal nutrition College of Animal Science and Technology China Agricultural University

Background: Clostridium perfringens is the main etiological agent of necrotic enteritis. Lactobacil i show beneficial effects on intestinal health in infectious disease, but the protective functions of lactobacil i in C. perfringens-infected chickens are scarcely described. This study examined the effects of Lactobacil us acidophilus(L. acidophilus) on the growth performance and intestinal health of broiler chickens chal enged with Clostridium perfringens(C. perfringens) over a 28-day period. Using a 2 × 2 factorial arrangement of treatments, a total of 308 1-day-old male Arbor Acres broiler chicks were included to investigate the effects of Lactobacil us acidophilus(L. acidophilus) on the growth performance and intestinal health of broiler chickens chal enged with Clostridium perfringens(C. perfringens) during a 28-day ***: During infection(d 14–21), C. perfringens challenge decreased the average daily gain(P < 0.05), and increased feed conversion ratio and the mortality rate(P < 0.05). However, dietary supplementation with L. acidophilus increased the body weight of C. perfringens-infected broilers on d 21(P < 0.05), and tended to decrease the mortality(P = 0.061).C. perfringens challenge decreased the villus height(P < 0.05), the ratio of villus height to crypt depth(P < 0.05) and OCLN(occludin) m RNA expression(P < 0.05), and increased the pro-inflammatory cytokine expression in the spleen and jejunum, the intestinal populations of C. perfringens and Escherichia(P < 0.05), and the serum content of endotoxin(P < 0.05), regardless of L. acidophilus supplementation. In contrast, dietary L. acidophilus reducedthe intestinal lesion score of challenged broilers(P < 0.05), the m RNA expression of pro-inflammatory cytokines, ileal populations of Escherichia and serum endotoxin content(P < 0.05), but increased the intestinal Lactobacillus populations(P < 0.05),irrespective of C. perfringens ***: Dietary addition of L. acidophilus could improve

关键词： Broiler Clostridium perfringens Intestine Lactobacillus acidophilus Necrotic enteritis

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Impact of dietary supplementation with β-alanine on the rumen microbial crude protein supply, nutrient digestibility and nitrogen retention in beef steers elucidated through sequencing the rumen bacterial community

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animal nutrition 2024年第2期17卷 418-427页

作者： Jinming Hu Shuo Zhang Mengmeng Li Guangyong Zhao state key laboratory of animal nutrition College of Animal Science and Technology China Agricultural University

This study investigated the effects of β-alanine(β-Ala) on rumen fermentation, nutrient digestibility,nitrogen(N) metabolism, plasma biochemical parameters, and rumen bacterial communities in beef steers. Six steers with initial liveweight of 252.8 ± 5.2 kg and 3 treatments of supplementing with 0, 30,or 60 g β-Ala per day to basal diet were allocated in a replicated 3 × 3 Latin square design. Each experimental period was 20 d, of which the first 15 d were for adaptation and the subsequent 5 d were for sampling. The results showed that β-Ala linearly increased the ruminal concentration of microbial crude protein(MCP)(P = 0.005), but it did not affect the ruminal concentrations of ammonia N and total volatile fatty acids(P > 0.10). β-Ala also linearly increased the dry matter(DM)(P = 0.009), organic matter(OM)(P = 0.017) and crude protein(CP)(P = 0.043) digestibility, tended to decrease the acid detergent fiber digestibility(P = 0.077), but it did not affect the neutral detergent fiber digestibility(P = 0.641). β-Ala quadratically increased the relative abundance of ruminal Bacteroidota(P = 0.021) at the phylum level, and increased Prevotella(P = 0.028) and Prevotellaceae＿UCG-003(P = 0.014), and decreased the relative abundance of NK4A214＿group(P = 0.009) at the genus level. Feeding steers with β-Ala linearly increased the urinary N(P = 0.006), urea excretions(P = 0.002) and the N retention(P = 0.004), but it did not affect the N utilization efficiency(P = 0.120). β-Ala quadratically increased the plasma concentration of the total antioxidant capacity(P = 0.011) and linearly increased the plasma concentration of insulin-like growth factor-1(P < 0.001). In summary, dietary supplementation with β-Ala improved the rumen MCP supply and increased the digestibility of DM, OM, CP and the N *** research is necessary to verify the ruminal degradability of β-Ala and to investigate the mechanism of the impact of absorbed β-Ala on the anti-oxidative ability in stee

关键词： β-Alanine Cattle Nitrogen metabolism Rumen bacterial community Rumen fermentation

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Ascorbic acid synthesis and transportation capacity in old laying hens and the effects of dietary supplementation with ascorbic acid

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Journal of animal science and Biotechnology 2019年第1期10卷 128-139页

作者： Liping Gan Hao Fan Wei Nie Yuming Guo The state key laboratory of animal nutrition College of Animal Science and TechnologyChina Agricultural University

Background: Laying hens over 75 weeks of age commonly show great declines in immunity and production *** is unclear whether these declines can be relieved by supplementing with ascorbic acid(AA) in *** trials were conducted to investigate the synthesis and metabolism of AA in layers of different ages and the effects of dietary supplemental AA on the performance and the immune and antioxidant statuses of 78 weeks old ***: In Exp.1,equal numbers(24 hens) of 35 weeks old(Young) and 75 weeks old(Old) layers were fed the same diet without AA supplementation for 4 *** Exp.2,360 healthy 78 weeks old laying hens were randomly assigned to 4 treatments(basal diet supplemented with 0,0.25,0.5,or 1 g AA/kg diet) in an 8-week feeding ***: The old hens tended to have decreased L-gulonolactone oxidase(GLO) synthase activity in the kidney and liver than that of the young hens(P = 0.07 and P = 0.05,respectively).Compared with the young hens,the old hens had lower hepatic antioxidant capacity allowing for the lower thioredoxin(TXN),thioredoxin reductase(TXNR) and cytochrome b5 reductase(CYB5 R) gene expression(P < 0.05),whereas increased sodium-dependent vitamin C transporter(SVCT) 1 expression levels in the ileum and kidney and enhanced splenic and hepatic AA concentrations(P < 0.05).Dietary supplementation with AA significantly decreased GLO enzyme activity but increased splenic AA concentration and anti-bovine serum albumin IgG levels(P < 0.05) and tended to increase CD4+T lymphocyte numbers(P = 0.06) in *** of 0.25 g AA/kg diet significantly increased hepatic total antioxidant capacity(T-AOC,P < 0.05) relative to the control ***: Laying hens could synthesize AA in both the kidney and the liver,though the GLO enzyme activities were 100 times greater in kidneys than in *** old laying hens had greater absorption and reabsorption capacity and higher AA retention in some tissues that did the young ***

关键词： Ascorbic acid Antioxidant capacity Immunity L-gulonolactone oxidase Old laying hens

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Effect of reduced energy density of close-up diets on dry matter intake,lactation performance and energy balance in multiparous Holstein cows

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Journal of animal science and Biotechnology 2015年第1期6卷 47-54页

作者： Wenming Huang Yujia Tian Yajing Wang Aminamu Simayi Amingguli Yasheng Zhaohai Wu Shengli Li Zhijun Cao state key laboratory of animal nutrition College of Animal Science and TechnologyChina Agricultural University

Energy intake prepartum is critically important to health, milk performance, and profitability of dairy cows. The objective of this study was to determine the effect of reduced energy density of dose-up diets on dry matter intake （DMI）, lactation performance and energy balance （EB） in multiparous Holstein cows which were housed in a free-stall barn and fed for ad libitum intake. Thirty-nine dry cows were blocked and assigned randomly to three groups fed a high energy density diet [HD, n = 13; 6.8 MJ of net energy for lactation （NEL）/kg; 14.0% crude protein （CP） ], or a middle energy density diet （MD, n = 13; 6.2 MJ NEh/kg; 14.0% CP）, or a low energy density diet （LD, n = 13; 5.4 MJ NEh/kg; 14.0% CP） from d 21 before expected day of calving. After parturition, all cows were fed the same lactation diet to d 70 in milk （DIM）. The DMI and NEE intake prepartum were decreased by the reduced energy density diets （P 〈 0.05）. The LD group consumed 1.3 last 24 h before calving. The milk yield and the postpartum kg/d （DM） more diet compared with HD group in the DMI were increased by the reduced energy density diet prepartum （P 〈 0.05）. The changes in BCS and BW prepartum and postpartum were not affected by prepartum diets HD group had higher milk fat content and lower lactose content compared with LD group during the first 3 wk of lactation （P 〈 0.05）. The energy consumption for HD, MD and LD groups were 149.8%, 126.2% and 101.1 % of their calculated energy requirements prepartum （P 〈 0.05）, and 72.7%, 73.1% and 7.5.2% during the first 4 wk postpartum, respectively. In conclusion, the low energy density prepartum diet was effective in controlling NF_L intake prepartum, and was beneficial in increasing DMI and milk yield, and alleviating negative EB postpartum.

关键词： Dietary energy density Dry matter intake Energy balance Lactation performance Transition cow

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Novel metabolic and physiological functions of branched chain amino acids: a review

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Journal of animal science and Biotechnology 2017年第3期8卷 501-512页

作者： Shihai Zhang Xiangfang Zeng Man Ren Xiangbing Mao Shiyan Qiao state key laboratory of animal nutrition College of Animal Science and Technology China Agricultural University college of animal science South China Agricultural University college of animal science Anhui Science & Technology University animal nutrition Institute Key Laboratory of Animal Disease-Resistance NutritionMinistry of Education Sichuan Agricultural University

It is widely known that branched chain amino acids（BCAA） are not only elementary components for building muscle tissue but also participate in increasing protein synthesis in animals and humans. BCAA（isoleucine, leucine and valine） regulate many key signaling pathways, the most classic of which is the activation of the m TOR signaling pathway. This signaling pathway connects many diverse physiological and metabolic roles. Recent years have witnessed many striking developments in determining the novel functions of BCAA including：（1） Insufficient or excessive levels of BCAA in the diet enhances lipolysis.（2） BCAA, especially isoleucine, play a major role in enhancing glucose consumption and utilization by up-regulating intestinal and muscular glucose transporters.（3）Supplementation of leucine in the diet enhances meat quality in finishing pigs.（4） BCAA are beneficial for mammary health, milk quality and embryo growth.（5） BCAA enhance intestinal development, intestinal amino acid transportation and mucin production.（6） BCAA participate in up-regulating innate and adaptive immune *** addition, abnormally elevated BCAA levels in the blood（decreased BCAA catabolism） are a good biomarker for the early detection of obesity, diabetes and other metabolic diseases. This review will provide some insights into these novel metabolic and physiological functions of BCAA.

关键词： Amino acid transporters Glucose transporters Gut health Immunity Lipolysis Mammary health Meat quality Milk production

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Effects of dietary phosphorous supplementation on laying performance,egg quality,bone health and immune responses of laying hens challenged with Escherichia coli lipopolysaccharide

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Journal of animal science and Biotechnology 2018年第4期9卷 980-990页

作者： Wei Nie Bo Wang Jing Gao Yuming Guo Zhong Wang state key laboratory of animal nutrition College of Animal Science and Technology China Agricultural University Department of animal science Washington State University

Background: Phosphorus is an essential nutrient to maintain poultry health and performance. The objective of this study was to evaluate the effect of dietary phosphorus levels on egg production, egg quality, bone health, immune responses of laying hens challenged with Escherichia coli ***: Three hundred laying hens at 28 wk were randomly divided into 2 dietary treatments with 10 replicates of 15 birds. The wheat-soybean based diets contained either 0.12% or 0.4% non-phytate phosphorus(NPP). At 32 wk of age, all the birds of each dietary treatment were injected into the abdomen with 1.5 mg/kg body weight(BW) of either LPS or saline once a day at 24-h intervals for continuous 9 d. The performance of laying hens was evaluated for 9 d. The eggs after the fifth injection were collected to value the egg quality. Three hours after the first injection, blood was collected to measure serum metabolite and immune response associated *** hours after the fifth injection, the hens were euthanized to obtain tibia, cecal tonsils and ***: Compared with saline-injected hens, LPS-injected hens had lower feed intake and egg production(P < 0.05).Eggshell thickness, strength, albumin height and Haugh unit were significantly increased in LPS-injected hens compared with saline-injected hens(P < 0.05). Furthermore, laying hens challenged with LPS had lower villious height/crypt depth ration than those received saline. Serum calcium, phosphorus and SOD activities significantly decreased in the LPS-injected hens compared with the control(P < 0.05). LPS up-regulated expression of IL-1β, IL-6 and IL-10 in cecum, and serum concentration of MDA, IL-1β and IL-6(P < 0.05), whereas 0.40% dietary non-phytate phosphorus supplementation significantly increased(P < 0.05) villi height/crypt depth ratio, decreased(P < 0.05) serum MDA and IFN-γ concentration compared with the 0.12% non-phytate phosphorus ***: In summary, this study demonstrates t

关键词： Egg quality Immune response Laying hens Lipopolysaccharide Phosphorus

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