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Journal of Cotton Research 2021年第2期4卷 147-159页

作者： MEHARI Teame Gereziher XU Yanchao MAGWANGA Richard Odongo UMER Muhammad Jawad KIRUNGU Joy Nyangasi CAI Xiaoyan HOU Yuqing WANG Yuhong YU Shuxun WANG Kunbo ZHOU Zhongli LIU Fang state key laboratory of Cotton Biology/Institute of Cotton Research Chinese Academy of Agricultural Sciences(ICRCAAS)Anyang455000HenanChina Ethiopian Institute of Agricultural Research Mekhoni Agricultural Research CenterP.O.Box 47MekhoniTigrayEthiopia School of Biological and Physical Sciences(SBPS) Main CampusJaramogi Oginga Odinga University of Science and Technology(JOOUST)Main CampusP.O.Box 210-40601BondoKenya School of Agricultural Sciences Zhengzhou UniversityZhengzhouHenan450001China

Background: Cotton is an important commercial crop for being a valuable source of natural *** production has undergone a sharp decline because of abiotic stresses,*** is one of the major abiotic stress causing significant yield losses in ***,plants have evolved self-defense mechanisms to cope abiotic factors like drought,salt,cold,*** evolution of stress responsive transcription factors such as the trihelix,a nodule-inception-like protein(NLP),and the late embryogenesis abundant proteins have shown positive response in the resistance improvement to several abiotic ***: Genome wide identification and characterization of the effects of Light-Harvesting Chloro a/b binding(LHC)genes were carried out in cotton under drought stress conditions.A hundred and nine proteins encoded by the LHC genes were found in the cotton genome,with 55,27,and 27 genes found to be distributed in Gossypium hirsutum,***,and ***,*** proteins encoded by the genes were unevenly distributed on various *** Ka/Ks(Non-synonymous substitution rate/Synonymous substitution rate)values were less than one,an indication of negative selection of the gene *** expressions of genes showed that majority of the genes are being highly upregulated in the roots as compared with leaves and stem *** genes were found to be highly expressed in MR-85,a relative drought tolerant ***: The results provide proofs of the possible role of the LHC genes in improving drought stress tolerance,and can be explored by cotton breeders in releasing a more drought tolerant cotton varieties.

关键词： Cotton G hirsutum LHC genes Gene expression Drought tolerance

Chromosome-scale assembly reveals asymmetric paleo-subgenome evolution and targets for the acceleration of fungal resistance breeding in the nut crop, pecan

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Plant Communications 2021年第6期2卷 96-110页

作者： Lihong Xiao Mengjun Yu Ying Zhang Jie Hu Rui Zhang Jianhua Wang Haobing Guo He Zhang Xinyu Guo Tianquan Deng Saibin Lv Xuan Li Jianqin Huang Guangyi Fan state key laboratory of Subtropical Silviculture Zhejiang A&F UniversityNo.666 Wusu St.Lin’an DistrictHangzhou 311300China BGI-Qingdao BGI-ShenzhenNo.2 Hengyunshan Rd.Huangdao DistrictQingdao 266555China BGI Genomics BGI-ShenzhenShenzhen 518083China

Pecan(Carya illinoinensis)is a tree nut crop of worldwide economic importance that is rich in healthpromoting ***,pecan production and nut quality are greatly challenged by environmental stresses such as the outbreak of severe fungal ***,we report a high-quality,chromosome-scale genome assembly of the controlled-cross pecan cultivar‘Pawnee’constructed by integrating Nanopore sequencing and Hi-C *** and evolutionary analyses reveal two whole-genome duplication(WGD)events and two paleo-subgenomes in pecan and *** estimates suggest that the recent WGD event and considerable genome rearrangements in pecan and walnut account for expansions in genome size and chromosome number after the divergence from *** two paleo-subgenomes differ in size and protein-coding gene *** exhibit uneven ancient gene loss,asymmetrical distribution of transposable elements(especially LTR/Copia and LTR/Gypsy),and expansions in transcription factor families(such as the extreme pecan-specific expansion in the far-red impaired response 1 family),which are likely to reflect the long evolutionary history of species in the Juglandaceae.A whole-genome scan of resequencing data from 86 pecan scab-associated core accessions identified 47 chromosome regions containing 185 putative candidate *** changes were detected in the expression of candidate genes associated with the chitin response pathway under chitin treatment in the scab-resistant and scabsusceptible cultivars‘Excell’and‘Pawnee’.These findings enable us to identify key genes that may be important susceptibility factors for fungal diseases in *** high-quality sequences are valuable resources for pecan breeders and will provide a foundation for the production and quality improvement of tree nut crops.

关键词： pecan genome assembly paleo-subgenome pecan scab fungal disease population genetics

Identifying genomic regions controlling ratoon stunting disease resistance in sugarcane(Saccharum spp.) clonal F_(1) population

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The crop Journal 2021年第5期9卷 1070-1078页

作者： Qian You Sushma Sood Ziliang Luo Hongbo Liu Md.Sariful Islam Muqing Zhang Jianping Wang state key Lab for Conservation and Utilization of Subtropical Agric-Biological Resources Guangxi UniversityNanning 530005GuangxiChina Agronomy Department University of FloridaGainesvilleFL 32610USA USDA-ARS Sugarcane Field StationCanal PointFL 33438USA Sugarcane Research Institute Yunnan Academy of Agricultural SciencesKaiyuan 661699YunnanChina Genetics Institute&Plant Molecular and Cellular Biology Program University of FloridaGainesvilleFL 32610USA

The ratoon stunting disease (RSD) of sugarcane,caused by the bacterium Leifsonia xyli ***,is one of the major concerns to sugarcane production and breeding programs *** to no obvious external symptoms,RSD cannot be easily detected by the growers,hence has reduced the world’s sugarcane production *** study aimed to identify quantitative trait loci (QTL) associated with RSD resistance and to assist in the development of linked molecular markers for marker-assisted breeding to minimize the reduction in sugarcane yield by the RSD infection.A set of 146 individuals derived from a self-crossing of CP80-1827 were evaluated for RSD resistance in a mechanically duplicated inoculated field trial from 2014 to 2017 using tissue blot *** the genetic data and the four years phenotyping data of CP80-1827 selfing population,linkage map construction and QTL analysis were conducted based on clonal F_1 and F_2 mapping population types with GACD V.1.2 and Ici Mapping V.3.3,respectively.A total of 23 QTL associated with RSD resistance were identified,which explained 6%to13%of the phenotypic variation with the two types of software.A total of 82 disease resistance genes were identified by searching these 23 QTL regions on their corresponding regions on the Sorghum bicolor genome (44 genes),sugarcane R570 genome (20 genes),and *** genome (18 genes),*** with Ici Mapping V.3.3,GACD V.1.2 identified more major (6 vs.3) and stable QTL (2vs.0),and more disease resistance genes (51 vs.31),indicating GACD V.1.2 (clonal F_1 mapping type) is most likely to be more efficient than Ici Mapping (F_2 mapping type) for QTL analysis of a sefling population or clonal F_1 population in clonal *** identified QTL controlling RSD resistance along with the associated SNP markers will assist sugarcane molecular breeding programs in combating this disease.

关键词： Quantitative trait loci(QTL) GACD IciMapping SNP marker Sugarcaneratoon stunting disease

Lodging resistance and yield potential of winter wheat:effect of planting density and genotype

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Frontiers of Agricultural Science and Engineering 2015年第2期2卷 168-178页

作者： Yonggui XIAO Jianjun LIU Haosheng LI Xinyou CAO Xianchun XIA Zhonghu HE Institute of crop Science National Wheat Improvement CenterChinese Academy of Agricultural Sciences(CAAS)Beijing 100081China crop Research Institute Shandong Academy of Agricultural SciencesJinan 250100China International Maize and Wheat Improvement Center(CIMMYT)China Office c/o CAASBeijing 100081China

Improved lodging resistance is important for achieving high yield in irrigated *** study was conducted to determine genotypic variation in lodging resistance and related morphological traits among winter wheat cultivars planted at two densities,and to identify key traits associated with lodging *** performance of 28 genotypes,including 24 released cultivars and four advanced lines,was evaluated at 250 plants per square meter and 500 plants per square meter in Shandong province during the 2008–2009 and 2009–2010 crop *** the higher density,the average grain yield was 2.6%higher,even though lodging score rose by as much as 136%.The higher planting density increased lodging through increased leaf area index(LAI),plant height,center of gravity and length of basal internodes,and reduced grain weight per spike and diameter of the lower two stem ***,center of gravity and diameter of first internodes,as the important indicators for lodging resistance,were significantly correlated with lodging score,with R=0.62,0.59 and–0.52(Presistance was significantly associated with diameter and length of the first internodes(R=0.71–0.77,Presistance in irrigated *** carrying high plant density tolerance and high yield potential,such as Jimai 22 and Liangxing 66,were recommended as leading cultivars for production as well as elite crossing parents for further increasing yield potential in the Yellow and Huai Valleys Winter Wheat Zone in China.

关键词： common wheat yield potential lodging performance pushing resistance leaf area index

Fungal diversity notes 253-366:taxonomic and phylogenetic contributions to fungal taxa

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Fungal Diversity 2016年第3期78卷 1-237页

作者： Guo Jie Li Kevin D.Hyde Rui Lin Zhao Sinang Hongsanan Faten Awad Abdel-Aziz Mohamed A.Abdel-Wahab Pablo Alvarado Genivaldo Alves-Silva Joseph F.Ammirati Hiran A.Ariyawansa Abhishek Baghela Ali Hassan Bahkali Michael Beug D.Jayarama Bhat Dimitar Bojantchev Thitiya Boonpratuang Timur S.Bulgakov Erio Camporesi Marcela CBoro Oldriska Ceska Dyutiparna Chakraborty Jia Jia Chen K.W.Thilini Chethana Putarak Chomnunti Giovanni Consiglio Bao Kai Cui Dong Qin Dai Yu Cheng Dai Dinushani A.Daranagama Kanad Das Monika C.Dayarathne Eske De crop Rafael J.V.De Oliveira Carlos Alberto Fragoso de Souza JoséIde Souza Bryn T.M.Dentinger Asha J.Dissanayake Mingkwan Doilom E.Ricardo Drechsler-Santos Masoomeh Ghobad-Nejhad Sean P.Gilmore Aristóteles Góes-Neto MichałGorczak Charles H.Haitjema Kalani Kanchana Hapuarachchi Akira Hashimoto Mao Qiang He John K.Henske Kazuyuki Hirayama Maria J.Iribarren Subashini C.Jayasiri Ruvishika S.Jayawardena Sun Jeong Jeon Gustavo H.Jerônimo Ana L.Jesus E.B.Gareth Jones Ji Chuan Kang Samantha C.Karunarathna Paul M.Kirk Sirinapa Konta Eric Kuhnert Ewald Langer Haeng Sub Lee Hyang Burm Lee Wen Jing Li Xing Hong Li Kare Liimatainen Diogo Xavier Lima Chuan Gen Lin Jian Kui Liu Xings Zhong Liu Zuo Yi Liu J.Jennifer Luangsa-ard Robert Lücking H.Thorsten Lumbsch Saisamorn Lumyong Eduardo M.Leaño Agostina V.Marano Misato Matsumura Eric H.C.McKenzie Suchada Mongkolsamrit Peter E.Mortimer Thi Thuong Thuong Nguyen Tuula Niskanen Chada Norphanphoun Michelle A.O’Malley Sittiporn Parnmen Julia Pawłowska Rekhani H.Perera Rungtiwa Phookamsak Chayanard Phukhamsakda Carmen L.A.Pires-Zottarelli Olivier Raspé Mateus A.Reck Sarah C.O.Rocha AndréL.C.M.Ade Santiago Indunil C.Senanayake Ledo Setti Qiu Ju Shang Sanjay K.Singh Esteban B.Sir Kevin V.Solomon Jie Song Prasert Srikitikulchai Marc Stadler Satinee Suetrong Hayato Takahashi Takumasa Takahashi Kazuaki Tanaka Li Ping Tang Kasun M.Thambugala Donnaya Thanakitpipattana Michael K.Theodorou Benjarong Thongbai Tuksaporn Thummarukcharoen Qing Tian Saowaluck Tibpromma Annemieke state key laboratory of Mycology Institute of MicrobiologyChinese Academy of SciencesNo 31st West Beichen RoadChaoyang DistrictBeijing 100101People’s Republic of China Centre of Excellence in Fungal Research and School of Science Mae Fah Luang UniversityChiang Rai 57100Thailand key laboratory for Plant Diversity and Biogeography of East Asia Kunming Institute of BotanyChinese Academy of ScienceKunming 650201YunnanPeople’s Republic of China Department of Botany and Microbiology College of ScienceKing Saud UniversityP.O.Box:2455Riyadh 1145Saudi Arabia Department of Botany and Microbiology Faculty of ScienceSohag UniversitySohag 82524Egypt ALVALAB C/La Rochela nº47E-39012 SantanderSpain Micolab Programa de Pós-Graduação em Biologia de FungosAlgas e PlantasDepartamento de BotânicaUniversidade Federal de Santa CatarinaCampus Universitário TrindadeCEP:88040-900 FlorianópolisSanta CatarinaBrazil Department of Biology University of WashingtonBox 351800SeattleWA 98195-1800USA Guizhou key laboratory of Agricultural Biotechnology Guizhou Academy of Agricultural SciencesGuiyang 550006GuizhouPeople’s Republic of China National Fungal Culture Collection of India(NFCCI) Biodiversity and Palaeobiology GroupMACS’Agharkar Research InstituteG.G.Agarkar RoadPune 411004India PO Box 116 HusumWA 98623-0116USA Formerly Department of BotanyGoa UniversityGoaIndia No.128/1-J Azad Housing SocietyCurcaP.O.Goa Velha 403108India *** 345 Shipwatch LaneHerculesCA 94547USA Microbe Interaction laboratory(BMIT) BIOTECNational Science and Technology Development Agency(NSTDA)113 Thailand Science ParkThanon PhahonyothinTombon Khlong NuengAmphoeKhlong LuangPathum Thani 12120Thailand Academy of Biology and Biotechnology Southern Federal UniversityRostov-on-Don 344090Rostov regionRussia A.M.B.Gruppo Micologico Forlivese BAntonio“Cicognani” Via Roma Forlì Italy 18 A.M.B.Circolo Micologico“Giovanni Carini”C.P.314BresciaItaly Societàper gli Studi Naturalistici della Romagna C.P.144Bagnacavallo

Notes on 113 fungal taxa are compiled in this paper,including 11 new genera,89 new species,one new subspecies,three new combinations and seven reference *** geographic and taxonomic range of fungal taxa are *** the Ascomycota the new genera Angustospora(Testudinaceae),Camporesia(Xylariaceae),Clematidis,Crassiparies(Pleosporales genera incertae sedis),Farasanispora,Longiostiolum(Pleosporales genera incertae sedis),Multilocularia(Parabambusicolaceae),Neophaeocryptopus(Dothideaceae),Parameliola(Pleosporales genera incertae sedis),and Towyspora(Lentitheciaceae)are *** introduced species are Angustospora nilensis,Aniptodera aquibella,Annulohypoxylon albidiscum,Astrocystis thailandica,Camporesia sambuci,Clematidis italica,Colletotrichum menispermi,***,Comoclathris pimpinellae,Crassiparies quadrisporus,Cytospora salicicola,Diatrype thailandica,Dothiorella rhamni,Durotheca macrostroma,Farasanispora avicenniae,Halorosellinia rhizophorae,Humicola koreana,Hypoxylon lilloi,Kirschsteiniothelia tectonae,Lindgomyces okinawaensis,Longiostiolum tectonae,Lophiostoma pseudoarmatisporum,Moelleriella phukhiaoensis,***,Mucoharknessia anthoxanthi,Multilocularia bambusae,Multiseptospora thysanolaenae,Neophaeocryptopus cytisi,Ocellularia arachchigei,***,Ochronectria thailandica,Ophiocordyceps karstii,Parameliola acaciae,***,Parastagonospora cumpignensis,Pseudodidymosphaeria phlei,Polyplosphaeria thailandica,Pseudolachnella brevifusiformis,Psiloglonium macrosporum,Rhabdodiscus albodenticulatus,Rosellinia chiangmaiensis,Saccothecium rubi,Seimatosporium pseudocornii,***,Sigarispora ononidis and Towyspora *** combinations are provided for Eutiarosporella dactylidis(sexual morph described and illus trated)and Pseudocamarosporium ***,illustrations and/or reference specimens are designated for Aposphaeria corallinolutea,Cryptovalsa ampelina,Dothiorella vidmadera,Ophiocordyceps formosana,P

关键词： Ascomycota Basidiomycota Neocallimastigomycota Oomycota.Zygomycota Phylogeny Taxonomy New genus New species

Quantitative Disease resistance： Dissection and Adoption in Maize

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Molecular Plant 2017年第3期10卷 402-413页

作者： Qin Yang Peter Balint-Kurti Mingliang Xu Department of Entomology and Plant Pathology Noah Carolina State University Raleigh NC 27895 USA National Maize Improvement Centre of China China Agricultural University Beijing 100193 People's Republic of China USDA-ARS Plant Sciences Research Unit Department of Entomology and Plant Pathology North Carolina State University Raleigh NC 27695 USA

Maize is the world＇s most produced crop, providing food, feed, and biofuel. Maize production is constantly threatened by the presence of devastating pathogens worldwide. Characterization of the genetic compo- nents underlying disease resistance is a major research area in maize which is highly relevant for resistance breeding programs. Quantitative disease resistance （QDR） is the type of resistance most widely used by maize breeders. The past decade has witnessed significant progress in fine-mapping and cloning of genes controlling QDR. The molecular mechanisms underlying QDR remain poorly understood and exploited. In this review we discuss recent advances in maize QDR research and strategy for resistance breeding.

关键词： quantitative disease resistance maize gene

Robust image processing algorithm for computational resource limited smart apple sunburn sensing system

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Information Processing in Agriculture 2020年第2期7卷 212-222页

作者： Guobin Shi Rakesh Ranjan Lav R.Khot Heilongjiang Bayi Agricultural University Daqing 163319People’s Republic of China Department of Biological Systems Engineering Center for Precision and Automated Agricultural SystemsWashington State UniversityProsserWAUSA

Heat and light stress causes sunburn to the maturing apple fruits and results in crop production and quality ***,when the fruit surface temperature(FST)rises above critical limits for a prolonged duration,the fruit may suffer several physiological disorders including *** manage apple sunburn,monitoring FST is critical and our group at Washington state University is developing a noncontact smart sensing system that integrates thermal infrared and visible imaging sensors for real time FST *** system needs to perform in-field imagery data analysis onboard a single board computer with processing unit that has limited computational ***,key objective of this study was to develop a novel image processing algorithm optimized to use available resources of a single board *** logic flow includes color space transformation,k-means++classification and morphological operators prior to fruit segmentation and FST *** developed algorithm demonstrated the segmentation accuracy of 57.78%(missing error=12.09%and segmentation error=0.13%).This aided successful apple FST estimation that was 10–18C warmer than ambient air ***,algorithm reduced the imagery data processing time cost of the smart sensing systemfrom 87 s to 44 s using image compression approach.

关键词： apple sunburn Fruit surface temperature Thermal-RGB imaging On-board image processing Kmeans++clustering Smart sensing systems

The use of shared haplotype length information for pedigree reconstruction in asexually propagated outbreeding crops, demonstrated for apple and sweet cherry

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Horticulture Research 2021年第1期8卷 2785-2797页

作者： Nicholas P.Howard Cameron Peace Kevin A.T.Silverstein Ana Poets James J.Luby Stijn Vanderzande Charles-Eric Durel Helene Muranty Caroline Denance Eric van de Weg Institut fur Biologie und Umweltwissenschaften Carl von Ossietzky UniversityOldenburgGermany Department of Horticultural Science University of MinnesotaSt.PaulMNUSA Department of Horticulture and Landscape Architecture Washington State UniversityPullmanWashingtonWAUSA Minnesota Supercomputing Institute University of MinnesotaMinneapolisMNUSA Universited’Angers Institut AgroINRAEIRHSSFR 4207 QuaSaVBeaucouzeFrance Plant Breeding Wageningen University and ResearchWageningenThe Netherlands

Pedigree information is of fundamental importance in breeding programs and related genetics ***,many individuals have unknown *** methods to identify and confirm direct parent–offspring relationships are routine,those for other types of close relationships have yet to be effectively and widely implemented with plants,due to complications such as asexual propagation and extensive *** objective of this study was to develop and demonstrate methods that support complex pedigree reconstruction via the total length of identical by state haplotypes(referred to in this study as“summed potential lengths of shared haplotypes”,SPLoSH).A custom Python script,HapShared,was developed to generate SPLoSH data in apple and sweet *** was used to establish empirical distributions of SPLoSH data for known relationships in these *** distributions were then used to estimate previously unknown *** studies in each crop demonstrated various pedigree reconstruction scenarios using SPLoSH *** cherry,a full-sib relationship was deduced for‘Emperor Francis,and‘Schmidt’,a half-sib relationship for‘Van’and‘Windsor’,and the paternal grandparents of‘Stella’were *** apple,29 cultivars were found to share an unknown parent,the pedigree of the unknown parent of‘Cox’s Pomona’was reconstructed,and‘Fameuse’was deduced to be a likely grandparent of‘McIntosh’.key genetic resources that enabled this empirical study were large genome-wide SNP array datasets,integrated genetic maps,and previously identified pedigree *** with similar resources are also expected to benefit from using HapShared for empowering pedigree reconstruction.

关键词： Python breeding crops

利用Fhb1基因功能标记选择提高黄淮冬麦区小麦品种对赤霉病的抗性

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作物学报 2018年第4期44卷 505-511页

作者：张宏军宿振起柏贵华张旭马鸿翔李腾邓云买春艳于立强刘宏伟杨丽李洪杰周阳中国农业科学院作物科学研究所/农作物基因资源与基因改良国家重大科学工程北京100081 河北省农林科学院粮油作物研究所河北石家庄050035 Department of Agronomy Kansas State UniversityManhattanKS 66506USA USDA-ARS/Hard Winter Wheat Genetics Research Unit ManhattanKS 66506USA 江苏省农业科学院/江苏省农业生物学重点实验室江苏南京210014 福建省南平市农业科学研究所福建建阳354200 新乡矮败小麦育种技术创新中心河南新乡453731 石家庄市农林科学研究院赵县试验基地河北赵县051530

赤霉病已上升为黄淮冬麦区的主要病害,提高小麦品种对赤霉病的抗性成为该麦区主要的育种目标之一。宁麦9号、生选6号、建阳798、建阳84、苏麦3号和宁麦13均携带Fhb1基因,对赤霉病表现中抗水平以上。本研究以这6个品种(系)为供体,分别与... 详细信息

赤霉病已上升为黄淮冬麦区的主要病害,提高小麦品种对赤霉病的抗性成为该麦区主要的育种目标之一。宁麦9号、生选6号、建阳798、建阳84、苏麦3号和宁麦13均携带Fhb1基因,对赤霉病表现中抗水平以上。本研究以这6个品种(系)为供体,分别与高感赤霉病的周麦16矮败小麦近等基因系杂交和回交,构建6个回交群体。利用Fhb1基因的KASP标记在回交后代中进行基因型分析,分别选择携带和不携带Fhb1基因的可育株,对后代株系进行单花滴注接种鉴定和田间病圃自然鉴定。回交后代携带Fhb1家系整体抗性达到中感,比不携带Fhb1家系的平均病小穗数低4.2(P<0.01),平均病情指数低4.0,比轮回亲本周麦16的平均病小穗数和病情指数分别低8.1(P<0.01)和28.4(P<0.01)。不同供体品种(系)回交后代在赤霉病抗性上表现出明显差异,以生选6号为供体的回交后代家系抗性表现最好。本研究表明,利用Fhb1基因分子标记辅助选择技术能够有效地提高黄淮冬麦区小麦品种的赤霉病抗性水平。

关键词：小麦赤霉病抗性 Fhb1基因分子标记辅助选择