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Engineered spidroin-derived high-performance fibers for diverse applications

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Nano Research 2024年第2期17卷 492-502页

作者： Dawen Qin Jingjing Li Huanrong Li Hongjie Zhang Kai Liu National-Local Joint Engineering laboratory for Energy conservation in Chemical Process Integration and Resources utilization Tianjin Key Laboratory of Chemical Process SafetySchool of Chemical Engineering and TechnologyHebei University of TechnologyTianjin 300130China state key laboratory of Rare Earth Resource utilization Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun 130022China Engineering Research Center of Advanced Rare Earth Materials Ministry of EducationDepartment of ChemistryTsinghua UniversityBeijing 100084China University of Science and Technology of China Hefei 230026China

Spider silks are well known for their exceptional mechanical properties that are tougher than Kevlar and steel.However,the restricted production amounts from their native sources limit applications of spider silks.Over the decades,there have been significant interests in fabricating man-made silk fibers with comparable performance to natural silks,inspiring many efforts both for biosynthesizing recombinant spider silk proteins(spidroins)in amenable heterologous hosts and biomimetic spinning of artificial spider silks.These strategies provide promising routes to produce high-performance and functionally optimized fibers with diverse applications.Herein,we summarize the hosts that have been applied to produce recombinant spidroins.In addition,the fabrication and mechanical properties of recombinant spidroin fibers and their composite fibers are also introduced.Furthermore,we demonstrate the applications of recombinant spidroin-based fibers.Finally,facing the challenges in biosynthesis,scalable production,and hierarchical assembly of high-performance recombinant spidroins,we give a summary and perspective on future development.

关键词： spider silk recombinant spidroin heterologous expression fiber fabrication mechanical property

Integrative multi-omics approaches reveal that Asian cultivated rice domestication influences its symbiotic relationship with arbuscular mycorrhizal fungi

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Pedosphere 2024年第2期34卷 315-327页

作者： Lei TIAN Jilin WANG Hongping CHEN Weiqiang LI Lam-Son Phan TRAN Chunjie TIAN key laboratory of Mollisols agroecology State Key Laboratory of Black Soils Conservation and UtilizationNortheast Institute of Geography and AgroecologyChinese Academy of SciencesChangchun 130102China Rice Research Institute Jiangxi Academy of Agricultural SciencesNanchang 330200China Institute of Genomics for Crop Abiotic Stress Tolerance Department of Plant and Soil ScienceTexas Tech UniversityLubbock TX 79409USA

Potential changes in the symbiotic relationship between rice(Oryza sativa)and microorganisms have occurred during the domestication of Asian cultivated rice(O.sativa)from common wild rice(Oryza rufipogon)and in response to global climate change,along with evolving adaptations to the environment.The potential genes may express differently or dominate the symbiotic relationships between arbuscular mycorrhizal fungi(AMF)and plants,which may be beneficial to rice breeding.To date,research on this important topic has been limited.In this study,we aimed to examine the symbiotic relationships of Asian common wild and cultivated rice species with AMF.By conducting a comparative metagenomic analysis of the rhizospheres of wild and cultivated rice species,we identified differences in Rhizophagus intraradices-related genes associated with wild and cultivated rice,as well as functional genes of AMF.Furthermore,we obtained root-related genes associated with AMF from transcriptome data of rice roots.Our results collectively suggest that R.intraradices-related genes in the rhizosphere of wild rice may be more conducive to its colonization.Additionally,bacteria from the Nitrosomonadaceae and Nitrospiraceae families identified in the rhizosphere of wild rice exhibited positive correlations with R.intraradices-related genes with protein identifiers 1480749 and1871253,which may indicate that nitrobacteria can enhance the functions of R.intraradices in association with wild rice.Next,in a case study using comparative transcriptome analysis of root samples obtained from R.intraradices-inoculated wild and cultivated rice plants,we found significantly higher expression levels of the strigolactone pathway-related genes DWARF3(D3)and DWARF14(D14)in R.intraradices-inoculated common wild rice than in R.intraradices-inoculated cultivated rice.This study provides a theoretical basis for identifying the effects of domestication on mycorrhizal symbiosis-related genes,which could be promoted in wild rice in the future.

关键词： Rhizophagus intraradices root-related gene symbiotic effect strigolactone wild rice

Surface pollen and modern vegetation in Southern Xinjiang,China

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Science China Earth Sciences 2024年第2期67卷 510-530页

作者： Yun ZHANG Lixin CHEN Yaoyao XI Zhaochen KONG Xianguo QIAO state key laboratory of Black Soils conservation and utilization Northeast Institute of Geography and AgroecologyChinese Academy of SciencesChangchun130102China Institute of Botany Chinese Academy of SciencesBeijing100093China key laboratory of Cenozoic Geology and Environment Institute of Geology and GeophysicsChinese Academy of SciencesBeijing100029China University of Chinese Academy of Sciences Beijing100049China School of Geosciences and Resources China University of Geosciences(Beijing)Beijing100083China

The study of modern pollen,and vegetation can improve the accuracy of paleoenvironmental reconstruction based on fossil pollen data.A total of 188 pollen surface samples were collected from Southern Xinjiang(34°00’–43°00’N,74°00’–94°00’E)in China,with an elevation range of 888–4530 m.Surface pollen and vegetation were studied in the northern region of the Kunlun Mountains and the southern region of the Tianshan Mountains.The results showed that the surface pollen assemblages on the southern slope of the Tianshan Mountains(3530–1124 m)were divided into three pollen zones,namely,montane steppe,montane desert steppe,and montane desert.These zones were dominated by Artemisia,Chenopodiaceae,and Ephedra pollen.The surface pollen assemblages in the eastern Pamir Plateau(4530–1287 m)could be divided into alpine meadow,montane desert steppe,and montane desert zones,which were predominantly characterized by Artemisia,Chenopodiaceae,and Asteraceae pollen.However,the Southern Tarim Basin(3548–888 m)comprised montane desert steppe and montane desert zones,which was dominated by Artemisia and Chenopodiaceae pollen.The geographic distribution of the surface pollen sites obtained using ArcGIS,the R-value,and Pearson correlation analyses indicated that the over-represented Picea and Pinus pollen were of external origin and were transported by wind,water(rivers),and ice(glacier movement).Over-representation of Ephedra and Nitraria pollen was mainly distributed in the montane desert and desert steppe zones,respectively.Pollen from Chenopodiaceae and Artemisia were over-represented and broadly distributed across Southern Xinjiang.Juglans pollen was concentrated in areas with walnut trees,strongly indicating the presence of local parent plants.According to the Hybrid Singleparticle Lagrangian Integrated Trajectory model,more Pinus pollen was transported to the Pamir region by external airflows during spring and summer,resulting in its higher surface pollen content.Research elucidating the relationship between modern pollen and vegeta

关键词： Surface pollen Vegetation Distribution Picea Southern Xinjiang

Electrochemical and colorimetric dual-signal detection of Staphylococcus aureus enterotoxin B based on AuPt bimetallic nanoparticles loaded Fe-N-C single atom nanocomposite

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Food Science and Human Wellness 2024年第4期13卷 2025-2035页

作者： Huan Liang Hongcheng Liu Haojian Lin Guobao Ning Xiaokang Lu Siying Ma Fei Liu Hui Zhao Canpeng Li School of Chemical Science and Technology Yunnan UniversityKey Laboratory of Medicinal Chemistry for Natural ResourceMinistry of EducationKunming 650091China Institute of Quality Standard and Testing Technology Yunnan Academy of Agricultural ScienceSupervision and Testing Center for Farm Product QualityMinistry of AgricultureKunming 650223China state key laboratory of Optoelectronic Materials and Technologies Guangdong Province Key Laboratory of Display Material and TechnologySchool of Electronics and Information TechnologySun Yat-sen UniversityGuangzhou 510275China laboratory for conservation and utilization of Bio-resource Yunnan UniversityKunming 650091China

Sensitive detection of Staphylococcus aureus enterotoxin B(SEB)is of importance for preventing food poisoning from threatening human health.In this work,an electrochemical and colorimetric dual-signal detection assay of SEB was developed.The probe(Ab2/AuPt@Fe-N-C)was bound to SEB captured by Ab1,where the Ab2/AuPt@Fe-N-C triggered methylene blue degradation and resulted in the decrease of electrochemical signal.Furthermore,the probe catalyzed the oxidation of 3,3’,5,5’-tetramethyl biphenyl to generate a colorimetric absorbance at 652 nm.Once the target was captured and formed a sandwich-like complex,the color changed from colorless to blue.SEB detection by colorimetric and electrochemical methods showed a linear relationship in the concentration ranges of 0.0002-10.0000 and 0.0005-10.0000 ng/mL,with limits of detection of 0.0667 and 0.1670 pg/mL,respectively.The dual-signal biosensor was successfully used to detect SEB in milk and water samples,which has great potential in toxin detection in food and the environment.

关键词： Staphylococcus aureus enterotoxin Electrochemical immunosensor Colorimetric assay MOF@borophene composite Dual-functional Fe-N-C signal atom catalyst

Risk assessment of agricultural green water security in Northeast China under climate change

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Science China Earth Sciences 2024年第7期67卷 2178-2194页

作者： Jingxuan SUN Guangxin ZHANG Yanfeng WU Liwen CHEN Peng QI Boting HU Yijun XU Northeast Institute of Geography and agroecology.Chinese Academy of Sciences Changchun 130102China University of Chinese Academy of Sciences Beijing 100049China state key laboratory of Black Soils conservation and utilization Northeast Institute of Geography and AgroecologyChinese Academy of SciencesChangchun 130102China School of Renewable Natural Resources Louisiana State University Agricultural Center227 Highland RoadBaton RougeLA 70803USA

Northeast China is an important base for grain production,dominated by rain-fed agriculture that relies on green water.However,in the context of global climate change,rising regional temperatures,changing precipitation patterns,and increasing drought frequency pose threats and challenges to agricultural green water security.This study provides a detailed assessment of the spatiotemporal characteristics and development trends of green water security risks in the Northeast region under the base period(2001-2020)and the future(2031-2090)climate change scenarios(SSP245 and SSP585)using the green water scarcity(GWS)index based on raster-scale crop spatial distribution data,Delta downscaling bias-corrected ERA5 data,and CMIP6 multimodal data.During the base period,the green water risk-free zone for dry crops is mainly distributed in the center and east of the Northeast region(72.4% of the total area),the low-risk zone is primarily located in the center(14.0%),and the medium-risk(8.3%)and high-risk(5.3%)zones are mostly in the west.Under SSP245 and SSP585 future climate change scenarios,the green water security risk shows an overall expansion from the west to the center and east,with the low-risk zone increasing to 21.6% and 23.8%,the medium-risk zone increasing to 16.0% and 17.9%,and the high-risk zone increasing to 6.9% and 6.8%,respectively.Considering dry crops with GWS greater than 0.1 as in need of irrigation,the irrigated area increases from 27.6%(base period)to 44.5%(SSP245)and 48.6%(SSP585),with corresponding increases in irrigation water requirement(IWR)of 4.64 and 5.92 billion m~3,respectively,which further exacerbates conflicts between supply and demand of agricultural water resources.In response to agricultural green water security risks,coping strategies such as evapotranspiration(ET)-based water resource management for dry crops and deficit irrigation are proposed.The results of this study can provide scientific basis and decision support for the development of Northeast irrigated agriculture and the cons

关键词： Climate change Food security Green water security risk Irrigation water requirement Green water scarcity

Constituents from leaves of Macaranga hemsleyana

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Chinese Herbal Medicines 2024年第3期16卷 481-486页

作者： Joseph Sakah Kaunda Jia Liu Yaojun Xu Yuansi Chen Chenfang Yue Xingjie Zhang Ruihan Zhang Muhammad Amin Weilie Xiao Hongliang Li Xiaoli Li key laboratory of Medicinal Chemistry for Natural Resource Ministry of EducationYunnan Characteristic Plant Extraction LaboratoryYunnan Provincial Center for Research & Development of Natural ProductsState Key Laboratory for Conservation and Utilization of Bio-Resources in YunnanSchool of PharmacySchool of MedicineChemical Science and TechnologyYunnan University Yunnan key laboratory of Pharmacology for Natural Products Haiyuan CollegeKunming Medical University

Objective:To study constituents of the leaves of Macaranga hemsleyana,and evaluate their inhibitory effects against NOD-like receptor thermal protein domain associated protein 3(NLRP3) inflammasome activation,and antiproliferative activity.Methods:The constituents were isolated and purified by column chromatography on MCI gel CHP20P/P120,silica gel,Sephadex LH-20,and HPLC.The structures of compounds were determined by 1D,2D NMR,and HR-ESI-MS data.The inhibitory effect of compounds on inflammasome activation was determined by lactate dehydrogenase(LDH) procedure.The antiproliferative activity was evaluated using MTT assay.Results:The study led to the isolation of 23 compounds,including one new compound,identified as(2Z)-3-[4-(β-D-glucopyranosyloxy)-2'-hydroxy-5'-methoxyphenyl]-2-propenoic acid(1),together with 22known compounds recognized as 1,4-dihydro-4-oxo-3-pyridinecarbonitrile(2),methyl 4-methoxynicotinate(3),4-methoxynicotinonitrile(4),1-(3-O-β-D-glucopyranosyl-4,5-dihydroxyphenyl)-ethanone(5),neoisoastilbin(6),isoastilbin(7),aromadendrin(8),neoastilbin(9),astilbin(10),quercitrin(11),neoschaftoside(12),apigenin 6,8-bis-C-α-L-arabinoside(13),vitexin(14),bergenin(15),scopoletin(16),glucopyranoside salicyl(17),koaburside(18),benzyl β-D-glucoside(19),icariside B5(20),roseoside(21),loliolide(22),and adenosine(23).The tested compounds did not show LDH inhibition nor antiproliferative activity.Conclusion:Compound 1 was a new glycoside.Compounds 2 and 3 were obtained for the first time from natural source.The 22 known compounds constituted of alkaloids(2-4,23),phenolics(5,15,17,18),flavonoids(6-14),coumarin(16),benzyl glycoside(19),and norsesquiterpenes(20-22).All the compounds,1-23,were revealed from M.hemsleyana for the first time.This is the initial uncovering of molecules 1-10,12,13,17-19,and 23 from the genus Macaranga,The isolated compounds,11,14-16,and 20-22established taxonomic classification of M.hemsleyana in Euphorbiaceae family.Flavonoids were outstanding as chemosystematic markers of Macaranga genus.

关键词： antiproliferative activity chemotaxonomic significance flavonoids glycoside Macaranga hemsleyana Pax et Hoffm NLRP3 inflammasome

A human-specific insertion promotes cell proliferation and migration by enhancing TBC1D8B expression

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Science China(Life Sciences) 2024年第4期67卷 765-777页

作者： Hui Zhao Lin-Lin Liu Jian Sun Lian Jin Hai-Bing Xie Jian-Bo Li Hui Xu Dong-Dong Wu Xiao-Lin Zhuang Min-Sheng Peng Ya-Jun Guo Wei-Zhu Qian Newton OOtecko Wei-Jie Sun Liang-Hu Qu Jie He Zhao-Li Chen Rong Liu Ce-Shi Chen Ya-Ping Zhang state key laboratory for conservation and utilization of Bio-resource School of Life SciencesSchool of Ecology and Environmental ScienceYunnan UniversityKunming 650091China School of Forensic Medicine Kunming Medical UniversityKunming 650500China Academy of Biomedical Engineering Kunming Medical UniversityKunming 650500China The Third Affiliated Hospital Kunming Medical UniversityKunming 650118China key laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences and Yunnan Province Kunming Institute of ZoologyChinese Academy of SciencesKunming 650201China state key laboratory of Genetic Resources and Evolution Yunnan Laboratory of Molecular Biology of Domestic AnimalsKunming Institute of ZoologyChinese Academy of SciencesKunming 650201China The key laboratory of Gene Engineering of the Ministry of Education State Key Laboratory for BiocontrolSun Yat-sen UniversityGuangzhou 510275China National Engineering Research Center for Antibody Medicine and Shanghai key laboratory of Cell Engineering and Antibody Shanghai 201203China Department of Thoracic Surgery Cancer Institute and HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China

Human-specific insertions play important roles in human phenotypes and diseases.Here we reported a 446-bp insertion(Insert-446)in intron 11 of the TBC1D8B gene,located on chromosome X,and traced its origin to a portion of intron 6 of the EBF1 gene on chromosome 5.Interestingly,Insert-446 was present in the human Neanderthal and Denisovans genomes,and was fixed in humans after human-chimpanzee divergence.We have demonstrated that Insert-446 acts as an enhancer through binding transcript factors that promotes a higher expression of human TBC1D8B gene as compared with orthologs in macaques.In addition,over-expression TBC1D8B promoted cell proliferation and migration through“a dual finger”catalytic mechanism(Arg538 and Gln573)in the TBC domain in vitro and knockdown of TBC1D8B attenuated tumorigenesis in vivo.Knockout of Insert-446 prevented cell proliferation and migration in cancer and normal cells.Our results reveal that the human-specific Insert-446 promotes cell proliferation and migration by upregulating the expression of TBC1D8B gene.These findings provide a significant insight into the effects of human-specific insertions on evolution.

关键词： human-specific insertion enhancer gene expression cell proliferation and migration

One stone two birds:electrochemical and colorimetric dual-mode biosensor based on copper peroxide/covalent organic framework nanocomposite for ultrasensentive norovirus detection

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Food Science and Human Wellness 2024年第2期13卷 920-931页

作者： Guobao Ning Quanmei Duan Huan Liang Huifang Liu Min Zhou Chunlan Chen Chong Zhang Hui Zhao Canpeng Li School of Chemical Science and Technology Yunnan UniversityKunming 650091China laboratory for conservation and utilization of Bio-resource Yunnan UniversityKunming 650091China Kunming Customs Technology Center Kunming 650228China

Norovirus(NoV)is regarded as one of the most common causes of foodborne diarrhea in the world.It is urgent to identify the pathogenic microorganism of the diarrhea in short time.In this work,we developed an electrochemical and colorimetric dual-mode detection for NoV based on the excellent dual catalytic properties of copper peroxide/COF-NH_(2)nanocomposite(CuO_(2)@COF-NH_(2)).For the colorimetric detection,NoV can be directly detected by the naked eye based on CuO_(2)@COF-NH_(2)as a laccase-like nonazyme using“peptide-NoV-antibody”recognition mode.The colorimetric assay displayed a wide and quality linear detection range from 1 copy/mL to 5000 copies/mL of NoV with a low limit of detection(LOD)of 0.125 copy/mL.For the electrochemical detection of NoV,CuO_(2)@COF-NH_(2)showed an oxidation peak of copper ion from Cu^(+)to Cu^(2+)using“peptide-NoV-antibody”recognition mode.The electrochemical assay showed a linear detection range was 1-5000 copies/mL with a LOD of 0.152 copy/mL.It's worthy to note that this assay does not need other electrical signal molecule,which provide the stable and sensitive electrochemial detection for NoV.The electrochemical and colorimetric dual-mode detection was used to detect NoV in foods and faceal samples,which has the potential for improving food safety and diagnosing of NoV-infected diarrhea.

关键词： Norovirus Specific peptides Electrochemical and colorimetric assay Dual-mode Copper peroxide/covalent organic framework

The DNA damage repair complex MoMMS21-MoSMC5 is required for infection-related development and pathogenicity of Magnaporthe oryzae

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Journal of Integrative Agriculture 2024年第6期23卷 1956-1966页

作者： Yue Jiang Rong Wang Lili Du Xueyu Wang Xi Zhang Pengfei Qi Qianfei Wu Baoyi Peng Zonghua Wang Mo Wang Ya Li state key laboratory of Ecological Pest Control for Fujian and Taiwan Crops Fujian Agriculture and Forestry UniversityFuzhou 350002China state key laboratory for conservation and utilization of Bio-Resources in Yunnan Yunnan Agricultural UniversityKunming 650201China Fujian Universities key laboratory for Plant-Microbe Interaction Fujian Agriculture and Forestry UniversityFuzhou 350002China Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops Minjiang UniversityFuzhou 350108China Fujian key laboratory for Monitoring and Integrated Management of Crop Pests Fuzhou 350003China

The conserved DNA damage repair complex,MMS21-SMC5/6(Methyl methane sulfonate 21-Structural maintenance of chromosomes 5/6),has been extensively studied in yeast,animals,and plants.However,its role in phytopathogenic fungi,particularly in the highly destructive rice blast fungus Magnaporthe oryzae,remains unknown.In this study,we functionally characterized the homologues of this complex,MoMMS21 and MoSMC5,in M.oryzae.We first demonstrated the importance of DNA damage repair in M.oryzae by showing that the DNA damage inducer phleomycin inhibited vegetative growth,infection-related development and pathogenicity in this fungus.Additionally,we discovered that MoMMS21 and MoSMC5 interacted in the nuclei,suggesting that they also function as a complex in M.oryzae.Gene deletion experiments revealed that both MoMMS21 and MoSMC5 are required for infection-related development and pathogenicity in M.oryzae,while only MoMMS21 deletion affected growth and sensitivity to phleomycin,indicating its specific involvement in DNA damage repair.Overall,our results provide insights into the roles of MoMMS21 and MoSMC5 in M.oryzae,highlighting their functions beyond DNA damage repair.

关键词： Magnaporthe oryzae MMS21 SMC5 DNA damage repair pathogenicity