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Microencapsulated chlorpyrifos: Degradation in soil and influence on soil microbial community structures

Journal of Environmental Sciences 2014年第11期26卷 2322-2330页

作者： Liezhong Chen Yanli Li Ting Wang Yali Jiang Kai Li Yunlong Yu College of Agriculture and Biotechnology Zhejiang University state key laboratory breeding base for Zhejiang Sustainable Pest and Disease Control Institute of Plant Protection and MicrobiologyZhejiang Academy of Agricultural Sciences

Degradation kinetics of microencapsulated chlorpyrifos （CPF-MC） in soil and its influence on soil microbial community structures were investigated by comparing with emulsifiable concentration of chlorpyrifos （CPF-EC） in laboratory. The residual periods of CPF-MC with fortification levels of 5 and 20 mg/kg reached 120 days in soil, both of the degradation curves did not fit the first-order model, and out-capsule residues of chlorpyrifos in soil were maintained at 1.76 （±0.33） and 5.92 （±1.20） mg/kg in the period between 15 and 60 days, respectively. The degradation kinetics of CPF-EC fit the first-order model, and the residual periods of 5 and 20 mg/kg treatments were 60 days. Bacterial community structures in soil treated with two concentrations of CPF-MC showed similarity to those of the control during the test period, as seen in the band number and relative intensities of the individual band on DGGE gels （p 〉 0.05）. Fungal community structures were slightly affected in the 5 mg/kg treatments and returned to the control levels after 30 days, but initially differed significantly from control in the 20 mg/kg treatments （p 〈 0.05） and did not recover to control levels until 90 days later. The CPF-EC significantly altered microbial community structures （p 〈 0.05） and effects did not disappear until 240 days later. The results indicated that the microcapsule technology prolonged the residue periods of chlorpyrifos in soil whereas it decreased its side-effects on soil microbes as compared with the emulsifiable concentration formulation.

关键词： pesticide Controlled-release formulation Non-controlled-release formulation Side effects Soil microbiology

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Polycephalomycetaceae,a new family of clavicipitoid fungi segregates from Ophiocordycipitaceae

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Fungal Diversity 2023年第3期120卷 1-76页

作者： Yuan‑Pin Xiao Yuan Bing Wang Kevin D.Hyde Gentekaki Eleni Jing‑zu Sun Yu Yang Juan Meng Hong Yu Ting‑Chi Wen state key laboratory breeding base of green pesticide and agricultural bioengineering Key Laboratory of Green Pesticide and Agricultural BioengineeringMinistry of EducationGuizhou UniversityGuiyang 550025China The Engineering Research Center of Southwest Bio‑Pharmaceutical Resources Ministry of EducationGuizhou UniversityGuiyang 550025GuizhouChina Center of Excellence in Fungal Research Mae Fah Luang UniversityChiang Rai 57100Thailand School of Science Mae Fah Luang UniversityChiang Rai 57100Thailand CAS key laboratory for Plant Diversity and Biogeography of East Asia Kunming Institute of BotanyChinese Academy of SciencesKunming 650201China Yunnan key laboratory for Fungal Diversity and green Development Kunming Institute of BotanyChinese Academy of SciencesKunming 650201China Yunnan Herbal laboratory School of Ecology and Environmental ScienceYunnan UniversityKunming 650091China Innovative Institute of Plant Health Zhongkai University of Agriculture and EngineeringHaizhu DistrictGuangzhou 510225China state key laboratory of Mycology Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China The Mushroom Research Centre Guizhou UniversityGuiyang 550025China School of Pharmaceutical Engineering Guizhou Institute of TechnologyGuiyang 550003GuizhouChina

Clavicipitoid fungi comprise three families,namely Clavicipitaceae,Cordycipitaceae,and *** are found worldwide and are specialized pathogens of invertebrate,plant and fungal *** the last decade,morphology-and phylogeny-based studies on clavicipitoid fungi have *** latter have revealed that Polycephalomyces,Perennicordyceps and Pleurocordyceps consistently cluster *** genera are currently considered as members of ***,information with regard to their diversity and ecology remains *** fill this gap,we collected 29 fresh specimens from insect and fungal substrates from tropical and subtropical evergreen forests in Thailand and southwestern *** performed detailed morphological analyses and constructed photoplates for all isolated *** used extensive taxon sampling and a dataset comprising internal transcribed spacer gene region(ITS),small subunit ribosomal RNA gene region(SSU),large subunit rRNA gene region(LSU),translation elongation factor 1-alpha gene region(TEF-1α),RNA polymerase II largest subunit gene region(RPB1)and RNA polymerase II second largest subunit(RPB2)to infer order-,family and genus-level phylogenetic *** on these biphasic analyses,we segregate Polycephalomyces,Perennicordyceps,and Pleurocordyceps from Ophiocordycipitaceae and introduce the new family Polycephalomycetaceae to accomodate these three *** majority of species in this family have a vast range of insect and fungal *** sexual morph of Polycephalomycetaceae has stromatic ascomata,long stipes,thick peridium,and cylindrical secondary *** asexual morph is characterized by colonies on the host surface or synnemata with stipes on the host,one or two types of phialides,and cylindrical to fusiform *** expand the number of taxa in the new family by introducing seven new species(Polycephalomyces albiramus,Perennicordyceps lutea,Pleurocordyceps parvicapitata,Pleurocordyceps lan

关键词： 8 new taxa Ophiocordyceps Polycephalomyces Hypocreales Clavicipitoid fungi Taxonomy New family Morphology Phylogeny

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Recent advances in formaldehyde-responsive fluorescent probes

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Chinese Chemical Letters 2017年第10期28卷 1935-1942页

作者： Zhiqiang Xu Jianhua Chen Lin-Li Hu Ying Tan Sheng-Hua Liu Jun Yin College of Chemistry Environmental Engineering Yangtze University fingzhou 434023 China The state key laboratory breeding base-Shenzhen key laboratory of Chemical Biology Graduate School at Shenzhen Tsinghua University Shenzhen 518055 China key laboratory of pesticide and Chemical Biology Ministry of Education College of Chemistry Central China Normal University Wuhan 430079 China

Formaldehyde, as one of the simplest reactive carbonyl species（RCS）, is regarded as a potential carcinogen and a sick house syndrome gas. Recent studies have shown that abnormally high levels of formaldehyde may result in cognitive decline and spatial memory deficits, asthmatic symptoms,Alzheimer＇s disease, and cancer. Due to the harmfulness of high levels of formaldehyde in nature and humans, it is of great significance to further elucidate the roles and functions of formaldehyde by a noninvasive detection approach. Fluorescence imaging has become a powerful and popular tool in monitoring bio-species owing to their high sensitivity and selectivity, excellent spatiotemporal resolution and non-invasion nature. Therefore, fluorescent probes are widely applied to track and detect formaldehyde in vitro and in vivo which have attracted more and more interest recently. This review focuses on various strategies to design the fluorescent probes for detecting formaldehyde based on different recognition groups.

关键词： Formaldehyde Fluorescent probes Bioimaging application Sensing mechanism

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green Plant Protection Innovation: Challenges and Perspectives

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Engineering 2020年第5期6卷 483-484页

作者： Baoan Song James NSeiber Stephen ODuke Qing XLi state key laboratory breeding base of green pesticide and agricultural bioengineering Key Laboratory of Green Pesticide and Agricultural BioengineeringMinistry of EducationGuizhou UniversityGuiyang 550025China Department of Environmental Toxicology University of California at DavisDavisCA 95616USA National Center for Natural Products Research Thad Cochran Research CenterSchool of PharmacyUniversity of MississippiOxfordMS 38655USA Department of Molecular Biosciences and bioengineering University of Hawaii at ManoaHonoluluHI 96822USA

This issue of Engineering was compiled from papers presented at the First International Conference on green Plant Protection Innovation(1st ICGPPI),held in Haikou in May 2018,and the Second International Conference on green Plant Protection Innovation(2nd ICGPPI),held in Guiyang in October *** conferences were sponsored by the Chinese Academy of Engineering,and the Academic Committee of the Forum of the ICGPPI,chaired by Professor Baoan Song,organized the *** 1st ICGPPI was attended by more than 800 people from seven countries,while the 2nd ICGPPI had more than 200 attendees from nine *** wide range of topics related to more environmentally sound crop protection was of great interest to all,and the meetings provided provided an opportunity for scientists from many disciplines of crop protection to exchange and share ideas and advances.

关键词： Plant green PPI

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Taxonomic studies of some often over-looked Diaporthomycetidae and Sordariomycetidae

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Fungal Diversity 2021年第6期111卷 443-572页

作者： Shi-Ke Huang Kevin D.Hyde Ausana Mapook Sajeewa S.N.Maharachchikumbura Jayarama D.Bhat Eric H.C.McKenzie Rajesh Jeewon Ting-Chi Wen state key laboratory breeding base of green pesticide and agricultural bioengineering Key Laboratory of Green Pesticide and Agricultural BioengineeringMinistry of EducationGuizhou UniversityGuiyang 550025China The Engineering Research Center of Southwest Bio-Pharmaceutical Resources Ministry of EducationGuizhou UniversityGuiyang 550025Guizhou ProvinceChina Center of Excellence in Fungal Research Mae Fah Luang UniversityChiang Rai 57100Thailand School of Science Mae Fah Luang UniversityChiang Rai 57100Thailand The Mushroom Research Centre Guizhou UniversityGuiyang 550025China Institute of Plant Health Zhongkai University of Agriculture and EngineeringHaizhu DistrictGuangzhou 510225China School of Life Science and Technology University of Electronic Science and Technology of ChinaChengdu 611731China Department of Botany Goa UniversityGoaIndia Present Address:Goa Velha India Manaaki Whenua-Landcare Research Private Mail Bag 92170AucklandNew Zealand Department of Health Sciences Faculty of Medicine and Health SciencesUniversity of MauritiusReduitMauritius

Sordariomycetes is an earlier-introduced and one of the widely distributed class of *** class was initially clas-sified based on morphology in having inoperculate and unitunicate *** the development of DNA based phylogenetic analysis,several undetermined or polyphyletic members of Sordariomycetes were ***,not all species belonging to this class have been sequenced and *** are a number of species,especially those old and poorly studied ones which have never been sequenced before and not even recollected again for further taxonomic *** of the main objective in this study is to revise and update the taxonomy of several well-known early and poorly studied spe-cies whose classification are still ***,we re-examined the type materials and/or authentic specimens together to explore 74 relatively poorly-studied genera,which mainly belong to Boliniales,Calosphaeriales,Chaetosphaeriales,Jobellisiales,and Sordariales classified under Diaporthomycetidae and *** provide descriptions,notes,figures and/or drawings and discussed their phylogenetic *** a result,the monotypic Jobellisiales is transferred from Hypocreomycetidae to *** on phylogenetic analysis,the polyphyletic Lasiosphaeriaceae is divided into five families,Bombardiaceae(Apodospora,Bombardia,Bombardioidea,Fimetariella and Ramophialophora),Lasiosphaeriaceae(Anopodium,Bellojisia,Corylomyces,Lasiosphaeria,Mammaria and Zopfiella),Lasiosphaeridaceae(Lasiosphaeris),Strattoniaceae(Strattonia)and Zygospermellaceae(Episternus and Zygospermella).In addition,a new family Neoschizotheciaceae is established based on *** of the type species of Boothiella,Stel-latospora,Sulcatistroma and Tengiomyces placed them in Sordariaceae,Chaetomiaceae,Hypocreales and Coronophorales,*** classify the genera lacking molecular data based on their morphology and expect them to be recollected;that is

关键词： 43 new taxa Boliniales Calosphaeriales Helminthosphaeriaceae Jobellisiales Sordariales Sordariomycetes

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Enhanced production of laccase by Coriolus hirsutus using molasses distillery wastewater

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Frontiers of Environmental Science & Engineering 2013年第2期7卷 200-210页

作者： Wei SUN Meiying XU ChunyuXIA Anhua LI Guoping SUN Guangdong Provincial key laboratory of Microbial Culture Collection and Application Guangdong Institute of Microbiology Guangzhou 510070 China Guangdong Open laboratory of Applied Microbiology Guangzhou 510070 China state key laboratory of Applied Microbiology (Ministry--Guangdong Province Jointly breeding base) South China Guangzhou 510070 China School of Bioscience and bioengineering South China University of Technology Guangzhou 510006 China

The effect of physical parameters of cultivation （load volume, temperature, pH, agitation, inoculum size, and incubation period） in the production of laccase by wood-rotting basidiomycete Coriolus hirsutus were stu- died using diluted molasses distillery wastewater （MDW） as a major composition. Using fractional factorial design, our study first identified load volume, agitation, and inoculum size as statistically significant factors. Optimal preferences and mutual interactions of the factors were then determined by the response surface method, which is based on the center composite design. A quadratic model was used to fit the experimental data. The optimized operational parameters for laccase production were determined to be the following： culture temperature of 25~C, pH 4, load volume of 40 mL diluted MDW in 150 mL flask, agitation rate of 183 r-m in-~, inoculation of 11.5% v/v, and cultivation time of 6 d. The experimental validation under these conditions （the maximum laccase production of ***-1 was within the confidence interval） subsequently verified the accuracy of the constructed model. Moreover, the removal of chemical oxygen demand and nitrogen of MDW reached 62.85% and 48.00% respectively, and the decolorization ratio under the optimal condition was 41.85%. The enhanced production of laccase by C. hirsutus is a new recovery strategy for MDW.

关键词： laccase Coriolus hirsutus fractional factorialdesign response surface method molasses distillery wastewater

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Degradation of Chlorpyrifos and Fipronil in Rice from Farm to Fork and Risk Assessment

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agricultural Sciences in China 2010年第5期9卷 754-763页

作者： ZHANG Cun-zheng ZHANG Xin-ming TIAN Zi-hua HE Dan-jun LIU Xian-jin Institute of Food Safety Jiangsu Academy of Agricultural Sciences/Key Laboratory of Food Safety Monitoring and Management Ministry of Agriculture/Key Lab of Food Quality and Safety of Jiangsu Province - State Key Laboratory Breeding Base Nanjing 210014 P.R. China Nanjing Centre of Inspection and Testing for agricultural Products Ministry of Agriculture Nanjing 210036 P.R.China Nanjing Exit-Entry Inspection and Quarantine Bureau Nanjing 210003 P.R.China Taicang Centre of Inspection and Testing for agricultural Products Taicang 215412 P.R.China

Degradation of pesticide residues （chlorpyrifos and fipronil） in rice from farm to fork and risk assessment for human health were studied to reveal the magnitude of risks faced by different populations of interest, so that appropriate measures can be taken to control the risks, and to refine and update the human health risk assessment data while helping to determine the maximum residue level （MRL） value and harvest interval. Different dosages and treatments were used in field trials for the harvest residue test. Residue levels of postharvest-applied chlorpyrifos and fipronil during storage, exposure to sunlight, washing and boiling processes （boiled rice） were investigated for brown rice. The dietary exposure evaluation model （DEEM） was employed to estimate acute and chronic risks faced by different populations of interest. Percent of reference dose （POR） and margin of exposure （MOE） were calculated. A positive correlation between pesticide residues and the dosage and application frequency of pesticide was found in the field trials. Risk quotients indicate that multiple applications and double dosages of chlorpyrifos increase the risks to the entire population and prolong exposures to toxic concentrations. The concentration of pesticide residues decreased as a function of time, after sunlight exposure, storage, washing, and boiling processes. 91.6 and 96.16% degradations were achieved at the end of the experimental period for fipronil and chlorpyrifos, respectively. The boiling process played an important role in the degradation of these pesticides. The result of risk assessment to human health showed that harvest residues of chlorpyrifos in rice and acute dietary risks of chlopyrifos were of concern. The acute dietary （food only） risk estimated for chlorpyrifos as percent of acute population adjusted dose （aPAD） was frequently over 100%. The risk faced by boys under the age of 14 was higher than that for girls of the same age. For the subpopulation above age 14, th

关键词： rice risk assessment pesticide residue chlorpyrifos fipronil

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Biosorption and biotransformation of crystal violet by Aeromonas hydrophila DN322p

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Frontiers of Environmental Science & Engineering 2013年第2期7卷 185-190页

作者： Tao PAN Suizhou REN Jun GUO Meiying XU Guoping SUN School of Bioscience and bioengineering South China University of Technology Guangzhou 510006 China Guangdong Institute of Microbiology Guangzhou 510070 China Guangdong Provincial key laboratory of Microbial Culture Collection and Application Guangdong Institute of Microbiology Guangzhou 510070 China Guangdong Open laboratory of Applied Microbiology Guangzhou 510070 China state key laboratory of Applied Microbiology (Ministry-Guangdong Province Jointly breeding base) South China Guangzhou 510070 China

DN322p, an offspring of Aeromonas hydro- phila DN322, has the capacity to adsorb and decolorize triphenylmethane dyes in wastewater simultaneously. As a common triphenylmethane dye, crystal violet （CV） was chosen to test the decolorization characteristics of DN322p. Within 0.5h, the strain DN322p adsorbed a large amount of CV, producing a deep-colored cell pellet and colorless supernatant. The colors of the cell pellet and supematant lightened over time. The supernatant and dichloromethane extract of the cell pellet both showed conspicuous CV and leuco CV （LCV） characteristic absorbance peaks at 590 nm and 260 nm, respectively, in the UV-vis spectral analysis. This finding indicated that the DN322p cells can adsorb the two dyes. A 99% （w/w） decolorization rate was achieved within 2.5 h with shaking at 30℃ for 50mg CV.L-1. High Performance Liquid Chromatography （HPLC） analysis of the dichloromethane extract of the supernatant and cell pellet confirmed that CV was mainly converted into its leuco form. Dead cells had a similar adsorption capacity with living cells. About 90% of CV in the dye solution （50mg-L-1） was removed by autoclaved cells with an optical delnsity at 600 nm （OD600） above 1.0.

关键词： crystal violet decolorization biosorption,biotransformation Aeromonas hydrophila DN322p

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Desymmetrization of Cyclic 1,3-Diketones under N-Heterocyclic Carbene Organocatalysis:Access to Organofluorines with Multiple Stereogenic Centers

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Research 2021年第1期2021卷 1495-1507页

作者： Guanjie Wang Min Zhang Yezhi Guan Ye Zhang Xianfang Hong Chenlong Wei Pengcheng Zheng Donghui Wei Zhenqian Fu Yonggui Robin Chi Wei Huang key laboratory of Flexible Electronics(KLOFE)&Institute of Advanced Materials(IAM)Nanjing Tech University(NanjingTech) 30 South Puzhu RoadNanjing 211816China College of Chemistry and green Catalysis Center Zhengzhou UniversityZhengzhouHenan 450001China School of Chemistry and Molecular Engineering Nanjing Tech University(NanjingTech)30 South Puzhu RoadNanjing 211816China laboratory breeding base of green pesticide and agricultural bioengineering Key Laboratory of Green Pesticide and Agricultural BioengineeringMinistry of EducationGuizhou UniversityHuaxi DistrictGuiyang 550025China Division of Chemistry&Biological Chemistry School of Physical&Mathematical SciencesNanyang Technological UniversitySingaporeSingapore 637371 Frontiers Science Center for Flexible Electronics(FSCFE)&Shaanxi Institute of Flexible Electronics(SIFE) Northwestern Polytechnical University(NPU)127 West Youyi RoadXi’an 710072China

Symmetric 1,3-diketones with fluorine or fluorinated substituents on the prochiral carbon remain to be ***,we have developed a novel prochiral fluorinated oxindanyl 1,3-diketone and successfully applied these substrates in carbene-catalyzed asymmetric ***,a versatile strategy for asymmetric generation of organofluorines with fluorine or fluorinated methyl groups has been *** stereogenic centers were selectively constructed with satisfactory *** diverse enantioenriched organofluorines were generated with excellent results in terms of yields,diastereoselectivities,and ***,exchanging fluorinated methyl groups to fluorine for this prochiral 1,3-diketones leads to switchable *** aspects and origin of stereoselectivity were studied by DFT ***,some of the prepared organofluorines demonstrated competitive antibacterial activities.

关键词： fluorine chiral asymmetric

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Modified pretreatment method for total microbial DNA extraction from contaminated river sediment

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Frontiers of Environmental Science & Engineering 2015年第3期9卷 444-452页

作者： Yun FANG Meiying XU Xingjuan CHEN Guoping SUN Jun GUO Weimin WU Xueduan LIU Guangdong Institute of Microbiology Guangzhou 510070 China School of Minerals Processing and bioengineering Central South University Changsha 410083 China key laboratory of Biometallurgy of Minislry of Education Changsha 410083 China state key laboratory of Applied Microbiology (Ministry-Guangdong Province Jointly breeding base) Guangzhou 510070 China Department of Civil & Environmental Engineering Center for Sustainable Development & Global Competitiveness Stanford University Stanford CA94305 USA

Extraction of high-quality microbial DNA from contaminated environmental samples is an essential step in microbial ecological study. based on previously published methods for soil and sediment samples, a modified pretreatrnent method was developed for extracting microbial DNA from heavily contaminated river sediment samples via selection of optimal pretreatment parameters （i.e., reagent solution, reaction duration, and temperature）. The pretreatment procedure involves wash ing the river sediment sample for three times with a solution containing 0.1 mol.L-1 ethylene diamine tetra- acetic acid （EDTA）, 0.1 mol- L-1 Tris （pH 8.0）, 1.5 mol. L1 NaC1, 0.1 mol. L-1 NaH2PO4, and Na2HPO4 at 65~C with ***-1 for 15min to remove humic materials and heavy metals prior to the employment of standard DNA extraction procedures. We compared the results of standard procedure DNA extraction following pretreatrnent, without pretreatment, and with using a commercial PowerSoilTM DNA Isolation Kit. The results indicated that the pretreatment significantly improved the DNA quality based on DNA yield, DNA fragment length, and determination of prokaryotic diversity. Prokaryotic diversity exhibited in the DNA with the pretreatment was also considerably higher than that extracted with the Power- SoilTM DNA Isolation Kit only. The pretreatment method worked well even with a small amount of sediment sample （0.25 g or even lower）. The method provides a novel, simple, cost-effective tool for DNA extraction for microbial community analysis in environmental monitoring and remediation processes.

关键词： river sediment DNA extraction contaminant,pretreatment microbial community

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